Frontiers in Bioengineering and Biotechnology
·
2024
·
IF 4.3
Yuting Li,Jingjing Guo,Shangde Sun
Recently, enzymatic method has been used to prepare biodiesel using various oils. But the high acid value of the biodiesel product using enzyme as a catalyst has been one issue. In this work, an attempt to reduce the acid value of fatty acid ethyl ester (FAEE) product to satisfy the specified requirement (AV ≤ 0.5 mgKOH/g), a complex enzyme-catalyzed method was used for the ethanolysis of Semen Abutili seed oil (SASO) (AV = 5.5 ± 0.3 mgKOH/g). The effects of various variables (constituents of complex enzyme, type and addition of water removal agent, time, temperature, enzyme addition load, substrate ratio) on the enzymatic reaction were investigated. The optimal reaction conditions were: 1% addition of liquid lipase Eversa ® Transform 2.0% and 0.8% of enzyme dry powder CALB, reaction temperature 35°C, alcohol-oil ratio 9:1 (mol/mol), 0.8 g/g of 4A-MS and reaction time 24 h. Under the optimal reaction conditions, the FAEE yield was 90.8% ± 1.5% and its acid value was decreased from 12.0 ± 0.2 mgKOH/g to 0.39 ± 0.10 mgKOH/g. In further evaluating the feasibility of preparing FAEE from SASO, the FAEE products obtained under the optimal reaction conditions were purified and evaluated with reference to the ASTM D6751 standard for the main physicochemical indexes. The results obtained were in accordance with the requirements except for the oxidative stability.
研究领域:
生物化学
·
可再生能源
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Le Wang,Pengxue Sun,Mingna Li,Yan Shen,Yangyong Lv,Hailong Tian,Dapeng Wu,Ying Liang,Qipeng Yuan,Jinshui Wang
A novel biological detoxification polymer (BDP) was in-situ synthesis by the co-immobilization of the compound enzymes (laccase and Aspergillus niger crude enzyme) and the aminated mesoporous silica nanoparticles (MSN-NH 2 ) with zeolitic imidazolate framework-8 (ZIF-8) for the detoxification of aflatoxin B 1 (AFB 1 ). The ZIF-8 shell of BDP acted as the AFB 1 adsorbent around the compound enzymes inside the BDP. The detoxification rate of BDP at 35 °C for 24 h was 96.7%. The detoxification of AFB 1 with the added mass ratio of BDP 1% to moldy corn was 65.8%. After the BDP was stored at 4 °C for 30 d, the enzyme activity remained 70.9%. Moreover, the enzyme activity and detoxification rate with BDP remained 80.7% and 80.0%, respectively, after the fourth repeated detoxification. The compound enzymes acted on the C–O bond and methoxyl group in the side chain of AFB 1 . Three degradation products (C 16 H 16 O 5 , C 16 H 14 O 4 , C 15 H 14 O 2 ) with different structures were detected in the degradation of AFB 1 with the BDP. Application of the in-situ BDP represents a promising way to improve the detoxification of AFB 1 .
研究领域:
食品科学
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生物技术
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环境科学
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产品关联
DOI
高影响因子
npj Parkinsons Disease
·
2025
·Vol. 11
·期 1
·1-10
·
IF 8.2
Lei Li,Chen Xiuli,Long Xincheng,Tu Qiuxia,Zhao Liping,Huang Dan,Song Lingli,Wang Jiaojiao,Zhai Suzhen,Chen Xiaozhong,Zhang Chunlin
Inspired by natural enzymes, artificial enzymes have garnered significant interest due to their simplicity of production, robustness under harsh conditions, and enhanced stability. This study introduces, for the first time, a novel Z/Ce@hemin composite enzyme, constructed by anchoring hemin onto zeolitic imidazolate framework-8 (ZIF-8)-encapsulated ceria (CeO 2 ) nanoparticles. This innovative design overcomes the challenges of hemin dimerization, enhances substrate affinity, and accelerates mass transport, leading to significantly improved peroxidase-like activity. The enzyme also demonstrates remarkable stability and resistance to environmental interference. Utilizing this Z/Ce@hemin composite, a sensitive and selective colorimetric detection system was developed for rapid and accurate quantification of hydrogen peroxide (H 2 O 2 ), a key oxidative stress marker. This approach was successfully applied in cellular models and Caenorhabditis elegans models of Parkinson’s disease, enabling precise monitoring of H 2 O 2 . The study provides a groundbreaking platform for oxidative stress modeling and expands possibilities in neurodegenerative disease research and therapeutic screening.
研究领域:
生物化学
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医学
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神经科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Shiqi Liu,Li Zhang,Mengshi Wang,Weiwei Liu,Ruiguo Cui,Bin Du,Lijun Song
Herein, Korla fragrant pear (KFP) was subjected to ultrasonication (UA), microwave (MA), high-pressure humid heat (HPHH), and composite enzyme hydrolysis (CEH) pretreatments before the extraction of soluble dietary fiber. The yield, structural characteristics, and functional properties of SDF obtained after the different pretreatments were compared. All the aforementioned pretreatment methods increased the yield of SDF and decreased that of insoluble dietary fiber. The highest yield of SDF was obtained after the HPHH pretreatment (10.11 %). The SDF obtained after pretreatment exhibited loose, cracked, and porous structures, decreased crystallinity and molecular weight (M w ), and improved physicochemical and functional properties. The highest water solubility (72.35 %), cation exchange capacity (0.92 mmoL/g), glucose adsorption capacity (7.80 mmoL/g), and cholesterol adsorption capacity (11.95 and 16.59 mg/g at pH 2 and 7, respectively), in vitro antioxidant activity, and α-glucosidase inhibitory activity were achieved following the CEH pretreatment. Multivariate analysis revealed that most of the functional properties were negatively correlated with the crystallinity and M w of SDF but positively correlated with the antioxidant activities (e.g., DPPH and ABTS) and α -glucosidase inhibitory activity. These findings provide a theoretical basis for the extraction, modification, and application of SDF extracted from KFP.
研究领域:
食品科学
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营养学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Yuying Hu,Zhuo Li,Xiaoqian Cao,Jiaqi Duan,Xin Shi,Can Wu,Bing Cui,Bin Zhou
This study aimed to examine the impact of composite enzymatic treatment on the physicochemical properties of oat milk, which would provide an effective strategy to improve the stability of plant-based milk. Oat milks treated with individual α-amylase or in combination with the protein glutaminase were produced. The result indicated that composite enzyme treatment significantly changed the physicochemical properties and significantly improved the stability of oat milk. Compared to original oat milk, a significant reduction in droplet size was observed in oat milk treated by α-amylase and composite enzymatic hydrolysis, with mean droplet size decreasing from 12.611 to 9.638 and 9.533 μm, respectively. And the microstructure of well-distributed droplets suggested that the aggregation in oat milk was effectively inhibited by composite enzymatic treatment of α-amylase (1.5 %) and protein glutaminase (0.3 %). Oat milk was characterized as a pseudoplastic fluid and dominated by elastic structure. Moreover, the rheological properties of oat milk were pronouncedly enhanced by enzymatic treatments, with a higher apparent viscosity and moduli presented. Physical stability revealed that within enzymatic treatment, especially for the composite enzymes, the long-term stabilization of oat milk was improved, presenting a lower TSI profile. Furthermore, the environmental stresses of oat milk subjected to composite enzymatic hydrolysis were evaluated, and suggested that this oat milk was relatively stable in neutral and alkaline conditions, also being stable in moderate temperature and ionic strength. Hence, this study demonstrated that the stabilization of oat milk was significantly improved by the composite enzymatic treatment, and exhibited good stability in environmental stresses, which was potentially useful in the application of plant-based milk. This study would provide valuable insights into improving the stability of oat milk in the further industry.
研究领域:
食品科学
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生物技术
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乳品科学
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植物基饮料
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产品关联
DOI
PubMed
高影响因子
ChemNanoMat
·
2023
·Vol. 9
·期 5
·e202200569
·
IF 3.8
Yanyu Li,Xiaoyue Zhang,Jinglin Shen,Wei Qi
Graphical A new type of nanocomposite enzymes has been developed, composed of Pd−Pt nanoparticles, hemin-rGO and CNTs-COOH. The nanocomposites show a high peroxidase-like activity and a linear response toward H 2 O 2 in the range of 0.05–500 μM with a determination limit of 0.01 μM. Based on this, a paper sensor was constructed for H 2 O 2 detection in real samples. Nanocomposite enzymes are attracting intensive attention worldwide. In the present study, a novel colorimetric H 2 O 2 paper sensor was developed based on a new type of nanocomposites composed of Pd−Pt nanoparticles, hemin functionalized reduced graphene oxide (hemin-rGO) and carboxylated carbon nanotubes (CNTs-COOH). The proposed nanocomposites show a high peroxidase-like activity. It provides a sensitive platform showing a linear response toward H 2 O 2 in the range of 0.05–500 μM with a determination limit of 0.01 μM. Based on this, the composites were applied to construct a paper sensor for H 2 O 2 detection in real samples. The cost-effectiveness, simplicity and efficiency of this paper sensor could be developed for “point-of-care” analysis and other related areas.
研究领域:
生物化学
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分析化学
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材料科学
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产品关联
DOI
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Zhiwei Zhang,Xinyi Yang,Zhenhong Gao,Meiyue Zhang,Shuaixue Mu,Yuying Cheng,Kunsheng Qu
Cucumbers produce by-products such as cucumber pomace during processing and most of them are discarded without being utilized. To effectively utilize the waste, cucumber pomace is used to extract both insoluble and soluble dietary fibers (DFs) using compound enzyme method (ME), High pressure processing assisted ME (HPP-ME), and dynamic high-pressure microfluidization-assisted ME (DHPM-ME). The results showed that DHPM-ME improved the extraction rate of soluble DFs most effectively, increasing it from 1.74 % to 4.08 %. The modified DFs exhibited enhanced hydration properties and functional properties after HPP-ME- and DHPM-ME-mediated auxiliary treatment. Additionally, the modified DFs exhibited improved thermal stability, increased absorption peaks in the infrared spectra, decreased crystallinity, improved glucose and cholesterol adsorption ability, and delayed glucose adsorption. The cucumber pomace-derived modified DFs can be used as a functional food additive in bakery, meat, dairy products, and beverages, and their effective use can further enhance the economic benefits.
研究领域:
食品科学
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生物技术
·
农业工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Science & Nutrition
·
2021
·Vol. 9
·期 6
·3078-3090
·
IF 2.863
Wang Wen-qiong,Zhou Ji-yang,Yu Qian,Li Jianju
In this study, enzymatic cross-linked whey protein coupling ultrafiltration was used to reduce membrane fouling and increase whey protein recovery rate. The filtration efficiency and protein interaction with the membrane surface were investigated. The results showed that the protein recovery rate and relative flux of transglutaminase catalysis protein followed by tyrosinase each increased by approximately 30% during ultrafiltration. The total membrane resistance was reduced by approximately 20%. The shape of the transglutaminase and tyrosinase cross-linked protein had somewhat spherical and cylindrical structure similar to an elongated shape based on fluorescence microscopy imaging, which indicated membrane resistance reduction. Fluorescence excitation–emission matrix spectroscopy (EEM) showed that the permeation peak intensities of transglutaminase followed by tyrosinase catalysis protein decreased sharply in the tryptophan and aromatic-like protein fields, indicating that most protein was rejected after ultrafiltration. The repulsive interaction energy was increased between the cross-linked proteins and membrane based on extended Derjaguin–Landau–Verwey–Overbeek (XDLVO) analysis.
研究领域:
生物技术
·
食品科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）