MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Xinyue Wang,Weiliang Zheng,Jiayi Liu,Yanyun Che,Ziqin Suo,Di Wu,Yunxian Zhou,Yingying Zeng,Xuemin Gao,Quancheng Chen,Qing Chen
Background Quantitative analysis of cholic acid (CA) is a vital component in the assessment of bile acids. Existing methods for the quantitative analysis of CA are often characterized by complicated operations or troublesome pretreatment procedures. To address these challenges, this study pioneers a novel approach by integrating molecular imprinting technology with electrochemical sensing technology. Results This innovative strategy has culminated in the development of a molecularly imprinted polymer (MIP)-based electrochemical sensor, which exhibits remarkable efficiency in detecting CA in complex matrices. The sensor demonstrates a limit of detection as low as 2.945 × 10 −3 μg/mL, along with a detection range spanning from 1 × 10 −2 μg/mL to 40 μg/mL. The sensor demonstrates a notable affinity for CA, exhibits high specificity in complex matrices, and consistently performs well in reproducibility experiments and stability tests. The sensor has also been shown to have the capability to detect CA accurately and quantitatively in real sample testing utilizing plasma as the matrix. The detection outcomes of TCM samples analyzed by the sensor exhibited minimal deviation from those of the conventional method, with no requirement for complex pretreatment and a straightforward operational procedure Significance In this study, molecular imprinting technology and electrochemical detection techniques were combined and applied to the detection of CA for the first time. This provided an efficient and economical method for detecting CA content in complex matrices. Furthermore, the sensor is straightforward to fabricate, convenient to carry, and rapid to measure in the field.
研究领域:
分析化学
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生物医学工程
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2015
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IF 4.169
Ruifen Jiang,Jianqiao Xu,Fang Zhu,Tiangang Luan,Feng Zeng,Yong Shen,Gangfeng Ouyang
Solid phase microextraction (SPME) has become a useful tool for in vivo monitoring the behavior of environmental organic pollutants in biological species due to its simplicity, relatively non-invasive, and cost-effective manner. However, the complex matrices in biological samples could significantly influence the extraction kinetic, and bias the quantification result. In this study, we investigated the effect of complex matrix on the extraction kinetic of SPME for biological sample analysis. Two sample matrices, phosphate-buffered saline (PBS) with bovine serum albumin (BSA) and agarose gel with BSA were used to simulate the biological fluid and tissue. Results showed that the addition of BSA significantly enhanced the mass transfer of organic compounds onto SPME fiber in both PBS buffer and gel sample. Enhancement factors ranging from 1.3 to 27, and 2.0 to 80 were found for all selected polyaromatic hydrocarbons (PAHs) in PBS buffer and agarose gel with BSA concentration of 0.1–5%, respectively. Then, an improved theoretical model was applied to quantify the observed enhancement effect, and the result showed that the predicted sampling time constant agreed well with the experimental one in complex matrix. Furthermore, a simplified equation was proposed for the real biological sample analysis.
研究领域:
分析化学
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环境科学
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生物医学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
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2024
·Vol. 191
·期 10
·1-13
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IF 5.3
Guo Liang,Zhou Shijin,Xue Jinyan,Liu Zenghui,Xu Shuqing,He Zhangxu,Yang Huaixia
A sandwich electrochemical sensor was fabricated based on multi-walled carbon nanotubes/ordered mesoporous carbon/AuNP (MWCNTs/CMK-3/AuNP) nanocomposites and porous core–shell nanoparticles Au@PdNPs to achieve rapid and sensitive detection of AFB 1 in complex matrices. MWCNTs/CMK-3/AuNP nanocomposite, which was prepared by self-assembly method, served as a substrate material to increase the aptamer loading and improve the conductivity and electrocatalytic activity of the electrode for the first signal amplification. Then, Au@PdNPs, which were synthesized by one-pot aqueous phase method, were applied as nanocarriers loaded with plenty of capture probe antibody (Ab) and signal molecule toluidine blue (Tb) to form the Au@PdNPs-Ab-Tb bioconjugates for secondary signal amplification. The sensing system could still significantly improve the signal output intensity even in the presence of ultra-low concentration target compound due to the dual signal amplification of MWCNTs/CMK-3/AuNP nanocomposites and Au@PdNPs-Ab-Tb. The method exhibited high selectivity, low detection limit (9.13 fg/mL), and strong stability to differentiate AFB 1 from other mycotoxins. Furthermore, the sensor has been successfully applied to the quantitative determination of AFB 1 in corn, malt, and six herbs, which has potential applications in food safety, quality control, and environmental monitoring. Graphical Abstract
研究领域:
分析化学
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食品安全
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环境监测
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纳米技术
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2020
·Vol. 413
·期 2
·389-401
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IF 3.637
Lu Yi Chen,Xiao Wei Wei,Wang Jun Yun,Xiong Xiao Hui
Because of its widespread distribution in the environment, bisphenol A (BPA) has become a global concern as an endocrine disruptor and a threat to human health through the food chain. Thus an efficient determination method is urgently needed for monitoring the levels of BPA. Herein, a novel electrochemical technique for the detection of BPA was performed by synchronous extraction and pre-concentration of BPA onto magnetic molecularly imprinted polymer (BMMIP), with subsequent readout on a magneto-actuated glassy carbon electrode (MGCE) by differential pulse voltammetry. Compared to the current methods of BPA determination, this BMMIP-based electrochemical sensor (BMMIPs@MGCE) not only simplifies the sample handling procedures substantially, without filtration, centrifugation, or other complex operations, but also can be easily renewed by a controllable magnetic field. As a sensor component, the core–shell BMMIPs exhibited excellent binding capacity (Q e = 82.5 mg g −1 ), short adsorption equilibrium time (30 s), and outstanding selectivity (k′ = 7.239) towards BPA, as well as stability and recyclability. Importantly, the BMMIPs@MGCE sensor was successfully applied for the on-site monitoring and rapid detection of BPA in complicated real-world specimens, with good recoveries (81.31–119.77%) and a low limit of detection (0.133 μmol L −1 ). Therefore, the stable and low-cost BMMIPs@MGCE sensor provides a new approach for the rapid determination of BPA in the field of environmental control and food safety.
Graphical abstract
研究领域:
环境科学
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分析化学
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食品安全
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产品关联
DOI
PubMed
高影响因子
TALANTA
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2020
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IF 5.339
Rui Gao,Dan Li,Siqing Zheng,Haixin Gu,Wei Deng
Accurate and selective in-field detection of metal ions in complex media has gained wide interests due to the complexed matrices and weak affinity towards sensing surface. Herein, we develop a first trimodal method for sensing of Zn 2+ in complex matrices by stimuli-responsive N-[6-piperazinyl-2-pyridinyl]-N-(2-pyridinylmethyl)-2-Pyridinemethanamine dithiocarbamates (DPY) modified gold nanorods (GNRs-DPY). The presence of Zn 2+ triggers the aggregation of GNRs-DPY, leading to increment of color and fluorescence intensity of the sensing system, which could be visually discerned with bare eye. Moreover, the intensive electromagnetic enhancement among “hot spots” of GNRs, generated during self-aggregation of the GNRs-DPY caused by Zn 2+ , lowers the detection limit of SERS assay to 6 × 10 −3 pM. It is noteworthy that GNRs-DPY based sensing platform not only enables distinguishing Zn 2+ from Cd 2+ , with simplicity and rapidity, but also demonstrates as trimodal nanoprobe for sensitive and selective quantitative determination of Zn 2+ in different matrices. Therefore, the GNRs-DPY provides a new strategy for accurate and credible on-spot determination of Zn 2+ in complicated specimens, as well as offering multiple applications in point-of-care monitoring.
研究领域:
分析化学
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纳米生物技术
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环境监测
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分子诊断
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金属离子传感
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2023
·
IF 4.8
Zheqi Fei,Yanyan Wang,Shengyu Cao,Dawei Lou,Xiyue Wang,Lili Lian
Efficient enrichment of trace organic phosphate ester (OPEs) pollutants from complex matrixes is essential for chromatographic analysis. Herein, oxygen-doped graphite carbon nitride (denoted as O-g-C 3 N 4 ) was fabricated though thermal polycondensation of urea using H 2 O 2 as an oxygen source. The resulting O-g-C 3 N 4 material was loose and had homogeneous pores structure, with a specific surface area of 73.74 m 2 /g. The O-g-C 3 N 4 was served as fiber coating material to create solid phase microextraction (SPME) device, with silica sol as adhesive for OPEs determination. Owing to the well-developed porosity of O-g-C 3 N 4 , which provides effective mass transfer channels for OPEs, the whole SPME procedure could be accomplished within 25 min. The O-g-C 3 N 4 fiber coating offers excellent mechanical strength and high stability and being workable after 180 sampling. The developed approach revealed a good linearity for OPEs in ultrapure water at the concentrations of 0.01–100 µg/L with the LODs between 0.001 µg/L and 0.003 µg/L. In addition, relative recoveries of 85.24% ∼99.73% were achieved in tap water and river water. These findings suggested that the oxygen-doped g-C 3 N 4 material has potential for headspace SPME of trace OPEs in various matrixes.
研究领域:
分析化学
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环境科学
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产品关联
DOI
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 13
·4895-4900
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IF 1.938
Jianqiao Xu,Shuming He,Ruifen Jiang,Fang Zhu,Jingwen Ruan,Hong Liu,Tiangang Luan,Gangfeng Ouyang
Recent developments of solid-phase microextraction (SPME) in biological and environmental analysis calls for robust and low-cost fibers that are prone to batch preparation with good reproducibility. However, the expensive commercial fibers and low reproducible home-made fibers cannot fully cater these requirements. In the present study, an extremely low-cost (less than one dollar) SPME fiber with good intra-fiber (RSDs% ≤ 1.6%, n = 6) and inter-fiber reproducibility (RSDs% ≤ 6.2%, n = 6) was prepared by mounting a piece of commercially available polydimethylsiloxane (PDMS) tubing on a stainless steel wire with epoxy glue. This configuration was stable for more than 100 extraction/thermal desorption cycles. In addition, compared with previously used thicker PDMS fiber, the capability of direct thermal desorption in gas chromatograph of the present fiber not only simplified the sample preparation process but also enhanced the analysis sensitivity. Excellent inter-fiber reproducibility and low cost even made the fiber disposable when used in complex matrices.
研究领域:
分析化学
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环境科学
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生物分析
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2017
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IF 7.78
Qian Qian Zhang,Bin Bin Chen,Hong Yan Zou,Yuan Fang Li,Cheng Zhi Huang
Hematin plays a crucial role in various physiological functions, and the determination of hematin in complex biological matrixes is a significant but difficult issue. Considering the unique photophysical/photochemical properties of carbon quantum dots (CQDs) prepared with p-aminobenzoic acid (PABA) and ethanol, a new strategy for the design of fluorescent probes for hematin has been achieved. The proposed sensor array is fabricated based on the inner filter effect (IFE) between hematin and CQDs with phenomenon of selective fluorescence quenching of CQDs which results from the strong absorption of the excitation and emission spectrum of CQDs by hematin. The fluorescence quenching of CQDs is closely related to the amount of hematin and there is a good linear relationship over the range of 0.5–10 μM with a detection limit of 0.25 μM. What's more, the fluorescence assay has been successfully applied for hematin sensing in healthy human red cells showing this sensing assay has a great potential prospect for detection of hematin in the complex matrixes .
研究领域:
分析化学
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生物医学工程
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光谱学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2016
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IF 7.476
Hui Yang,Yang Gao,Siqi Wang,You Qin,Lu Xu,Dan Jin,Fan Yang,Guo-Jun Zhang
In this study, we report an enzyme-free and conjugation-free electrochemical genosensor enabling an ultrasensitive readout of BRCA-1, a breast cancer susceptibility gene. The sensor employs a target-responsive hybridization chain reaction (HCR) to significantly amplify the detectable current signals. By means of a functional auxiliary probe pair and a versatile initiator sequence, a linear DNA concatemer structure can be formed via spontaneous and continuous polymerization of DNA oligomers in the presence of target sequence. Such a DNA nanoassembly endows the genosensor an ultrahigh sensitivity up to 1 aM, which is higher than that of the nanomaterials-based or enzyme mediated amplification approaches by several orders of magnitude. More importantly, the sensor’s responsive peak current exhibits a favorable linear correlation to the logarithm of the concentrations of target sequence ranging from 1 aM to 10 pM. In addition, the sensor is highly selective, and can discriminate a single mismatched sequence. This HCR-based genosensor is also capable of probing low-abundance BRCA-1 gene sequence directly in complex matrices , such as 50% human serum, with minimal interference. These advantages will make our tailor-engineered HCR-based electrochemical genosensor appealing to genetic analysis and clinical diagnostics.
研究领域:
遗传学
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生物医学工程
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分析化学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2024
·
IF 8
Yuqing Gan,Xi Long,Zan Gong,Panpan Yuan,Yalan Tang,Shian Zhong,Yanjing Yang
Given the significant threat to human health posed by Staphylococcus aureus , rapid and accurate quantification of S. aureus is essential to prevent and diagnose foodborne diseases and to ensure the safety of food consumption. Here, we developed a novel, simple，and label-free biosensor for quantitatively detecting S . aureus by integrating an allosteric aptamer probe and a rolling circle amplification technique. Pathogen S . aureus-specific binding could induce the structural change of the allosteric aptamer beacon to expose the primer for the padlock probe. Then, the primer could trigger the RCA reaction to produce a long-chain singles-tranded DNA harboring repeating Thioflavin T (ThT) aptamer, leading to powerful fluorescence signals. The assay demonstrates sensitivity, specificity, and accuracy, showing its potential as an alternative tool for pathogen detection in different fields, such as food safety monitoring.
研究领域:
微生物学
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生物技术
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食品科学
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分析化学
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DOI
高影响因子
Nature Protocols
·
2021
·Vol. 16
·期 9
·4201-4226
·
IF 13.491
Gao Pengcheng,Wang Dagui,Che Cheng,Ma Qun,Wu Xiaoqing,Chen Yajie,Xu Hongquan,Li Xinchun,Lin Yu,Ding Defang,Lou Xiaoding,Xia Fan
Solid-state nanochannels (SSNs) provide a promising approach for biosensing due to the confinement of molecules inside, their great mechanical strength and diversified surface chemical properties; however, until now, their sensitivity and specificity have not satisfied the practical requirements of sensing applications, especially in complex matrices, i.e., media of diverse constitutions. Here, we report a protocol to achieve explicit regional and functional division of functional elements at the outer surface (FE OS ) and inner wall (FE IW ) of SSNs, which offers a nanochannel-based sensing platform with enhanced specificity and sensitivity. The protocol starts with the fabrication and characterization of the distribution of FE OS and FE IW . Then, the evaluation of the contributions of FE OS and FE IW to ionic gating is described; the FE IW mainly regulate ionic gating, and the FE OS can produce a synergistic effect. Finally, hydrophobic or highly charged FE OS are applied to ward off interference molecules, non-target molecules that may affect the ionic signal of nanochannels, which decreases false signals and helps to achieve the highly specific ionic output in complex matrices. Compared with other methods currently available, this method will contribute to the fundamental understanding of substance transport in SSNs and provide high specificity and sensitivity in SSN-based analyses. The procedure takes 3–6 d to complete.
研究领域:
纳米技术
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生物传感器
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材料科学
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分析化学
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DOI
PubMed
高影响因子
Food Analytical Methods
·
2025
·
IF 3
Hu Jianan,Hong Dilong,Zhang Huan,Jiang Yuliang
Tryptophan, an essential amino acid for the human body, plays a crucial role in various physiological processes, including protein synthesis, fatty acid metabolism, enhancing energy levels, and being converted into neurotransmitters to regulate sleep and mood. It is also used in the prevention and treatment of pellagra. However, excessive intake of tryptophan can cause adverse effects, so detecting its concentration is of great significance. In this paper a silicon-based carbon quantum dot (N,Si-CDs) tryptophan probe was prepared. The structure and composition of N,Si-CDs were characterized by TEM, FT-IR, and XPS. Fluorescence studies revealed that N,Si-CDs exhibited high selectivity for tryptophan with minimal interference from other substances. There was a good Linear relationship between the fluorescence intensity of N,Si-CDs and the concentration of tryptophan in the range of 5–25 μM, and the detection Limit was as low as 19 nM. N,Si-CDs were successfully applied to detect tryptophan in complex Matrices such as honey, Yili milk powder, water-soluble vitamin C, sour plum soup, and lemon tea, with recovery rates ranging from 81.3% to 106.4%. Cell experiments demonstrated that N,Si-CDs had low toxicity to T24 cells and could be used for live-cell imaging. This N,Si-CDs shows great potential in fields such as food safety, environmental monitoring, and biomedical research.
研究领域:
分析化学
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纳米材料
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生物传感器
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食品安全
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生物医学研究
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环境监测
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）