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Foods · 2023 ·Vol. 12 ·期 18 ·3414 · IF 5.2

Metabolome and Microbiome Analysis to Study the Flavor of Summer Black Tea Improved by Stuck Fermentation

Lianghua Wen,Lingli Sun,Ruohong Chen,Qiuhua Li,Xingfei Lai,Junxi Cao,Zhaoxiang Lai,Zhenbiao Zhang,Qian Li,Guang Song,Shili Sun,Fanrong Cao

Tea is the most popular and widely consumed beverage worldwide, especially black tea. Summer tea has a bitter and astringent taste and low aroma compared to spring tea due to the higher content of polyphenols and lower content of amino acids. Microbial fermentation is routinely used to improve the flavor of various foods. This study analyzed the relationship between the quality of black tea, metabolic characteristics, and microbial communities after microbial stuck fermentation in summer black tea. Stuck fermentation decreased the bitterness, astringency sourness, and freshness, and increased the sweetness, mellowness, and smoothness of summer black tea. The aroma also changed from sweet and floral to fungal, with a significant improvement in overall quality. Metabolomics analysis revealed significant changes in 551 non-volatile and 345 volatile metabolites after fermentation. The contents of compounds with bitter and astringent taste were decreased. Sweet flavor saccharides and aromatic lipids, and acetophenone and isophorone that impart fungal aroma showed a marked increase. These changes are the result of microbial activities, especially the secretion of extracellular enzymes.Aspergillus,Pullululanibacillus, andBacilluscontribute to the reduction of bitterness and astringency in summer black teas after stuck fermentation, and Paenibacillus andBasidiomycota_gen_Incertae_sediscontribute positively to sweetness. In addition, Aspergillus was associated with the formation of fungal aroma. In summary, our research will provide a suitable method for the improvement of tea quality and utilization of summer tea, as well as provide a reference for innovation and improvement in the food industry.Keywords:summer black tea;tea quality;metabolic profile;microbial community

研究领域: 食品科学 · 微生物学 · 化学生态学 · 农学
✓ 已验证 DOI PubMed 高影响因子
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Sustainability · 2021 ·Vol. 13 ·期 23 ·13244 · IF 3.251

Purification Effects on β-HCH Removal and Bacterial Community Differences of Vertical-Flow Constructed Wetlands with Different Vegetation Plantations

Qing Chen,Honghu Zeng,Yanpeng Liang,Litang Qin,Guangsheng Peng,Liangliang Huang,Xiaohong Song

This study aimed to investigate the removal of β-hexachlorocyclohexane (β-HCH) at realistic concentration levels (10 µg/L) in different plant species in constructed wetlands (Acorus calamus,Canna indica,Thalia dealbata, andPontederia cordata) and the structure of the rhizosphere microbial community response of each group during summer and winter. Results showed that all groups of constructed wetlands had very good decontamination efficiency against β-HCH in water (90.86–98.17%). The species that most efficiently purified β-HCH in water wasA. calamusin summer (98.17%) andC. indicain winter (96.64%). Substrate sorption was found to be the major pathway for β-HCH removal from water in the constructed wetlands. The ability of the wetland plants to absorb and purify β-HCH was limited, andC. indicahad the strongest absorptive capacity among the four plant species. The mean β-HCH removal from the matrix of the planted plants increased by 5.8% compared with that of the control treatment (unplanted plants). The average β-HCH content in the plant rhizosphere substrate was 4.15 µg/kg lower than that in the non-rhizosphere substrate. High-throughput sequencing analysis revealed significant differences (P< 0.05) in the Chao1 and ACE indices of microbes in the substrate of four wetlands during summer and winter. At the genus level, the constructed wetlands with vegetation plantations showed higher microbial abundance than the constructed wetlands without vegetation plantations. In winter, the bacterial community structure of each constructed wetland was quite different, but no dominant flora in the bacterial community structure obviously changed. In summer, the bacterial community structure at the same stage was relatively small. The abundance ofActinobacteriaandSphingomonasremarkably increased over time in summer.Keywords:β-HCH;purification effects;vertical-flow constructed wetlands;bacterial community

CAS / MDL: 75-09-2 141-78-6
研究领域: 环境科学 · 生态学 · 微生物学 · 水处理
✓ 已验证 产品关联 DOI 高影响因子
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FOOD CHEMISTRY · 2022 · IF 9.231

Improving the flavor of summer green tea (Camellia sinensis L.) using the yellowing process

Yuming Wei,Xuchao Yin,Huiting Wu,Mengjie Zhao,Junlan Huang,Jixin Zhang,Tiehan Li,Jingming Ning

Summer green tea (SGT) has poor flavor due to its high levels of bitterness and astringency. The present study aimed to improve the flavor of SGT using the yellowing process. The results showed that after the yellowing process, the sweetness and overall acceptability increased, and the content of gallated catechins and flavonol glycosides decreased by 30.2% and 27.4%, respectively, as did the bitterness and astringency of SGT. Yellowing caused a decrease in the concentration of some aroma compounds, such as (z)-3-hexen-1-ol, 1-hexanol, pentanal, heptanal and 1-octanol, which caused grassy, floral and fruity aromas. In contrast, the concentrations of 1-octen-3-ol, benzene acetaldehyde and β-ionone increased, which have mushroom and sweet aromas. Meanwhile, the sweetness and umami of SGT were enhanced by the addition of selected aroma compounds (1-octen-3-ol, benzene acetaldehyde and β-ionone), demonstrating that the yellowing process improves the flavor of SGT through odor–taste interactions.

CAS / MDL: 110-38-3
研究领域: 食品科学 · 茶叶加工 · 风味化学
✓ 已验证 产品关联 DOI PubMed 高影响因子
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CHEMICAL PAPERS · 2024 ·Vol. 78 ·期 5 ·3367-3380 · IF 2.1

Controllable preparation of PDMS-based copolymers with long-lasting high hydrophobicity for coating protection of summer cloth surfaces

Dong Yalin

Cool linen is used to produce summer clothing to be known as summer cloth. Nevertheless, ramie-fiber surfaces bear hydrophilic hydroxyls to result in rainwater wetting and stain adhering. To achieve outdoor application of summer cloth, here, light all-organic hydrophobic copolymer coatings were formed on surfaces of two various summer clothes. First, atom transfer radical polymerization was utilized to synthesize polydimethylsiloxane-block-polymethacryloxypropyl trimethoxysilane. Then, neat copolymer films and copolymer coating/cloth complexes were prepared. Coating/cloth complexes were characterized. Finally, hydrophobicity-induced antifouling properties of complexes were confirmed, followed by justifying good resistance to surface abrasion for coating/compact summer cloth complex with superhydrophobicity. As a result, the optimal coating/compact summer cloth complex can fight against ink pollution and exhibit a water contact angle of 150.3 degrees after 200 abrasion cycles, in which neat copolymer film has a light transmittance of ~ 90% based on 30 min of pre-hydrolysis. Superhydrophobicity is ascribed to silicon surface enriching-induced low surface energy as well as surface micro-/nano-binary composite rough structure. This work has realized a controllable preparation of silicon-bearing copolymers with long-term hydrophobicity for protecting various summer clothes.

研究领域: 材料科学 · 高分子化学 · 表面工程 · 纺织科学 · 涂层技术
✓ 已验证 DOI 高影响因子
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Antioxidants · 2025 ·Vol. 14 ·期 6 ·630 · IF 6.6

Melatonin Improves Semen Quality by Modulating Oxidative Stress, Endocrine Hormones, and Tryptophan Metabolism of Hu Rams Under Summer Heat Stress and the Non-Reproductive Season

Qian-Qiu Liu,Xiong Li,Jia-Hao Li,Yang Zhou,Ming-Kai Lei,Wei-Qi Yin,You-She Ren,Chun-He Yang,Chun-Xiang Zhang

The concurrent occurrence of summer heat stress and the non-breeding season has significantly impaired the semen quality of rams. Currently, there exists no straightforward and efficient method to address this issue. In this study, we demonstrate that two consecutive administrations of melatonin implants significantly mitigate the adverse effects of summer heat stress and the non-reproductive season on rams. Our findings indicate that implantation of exogenous melatonin enhances semen quality by improving sperm DNA integrity, mitochondrial integrity, and decreasing the proportion of abnormal sperm, as compared to control rams. This improvement is ascribed to the alleviation of oxidative stress and the optimized regulation of endocrine hormone levels in both serum and seminal plasma. Further exploration of the regulatory mechanism reveals that melatonin can also influence the tryptophan metabolism pathway. Additionally, our study revealed that certain indices and metabolites are strongly correlated with semen quality and can potentially serve as indicators for research aimed at improving semen quality. Notably, this is the first time that differences in tryptophan metabolites between serum and seminal plasma have been elucidated. All the above information suggests that melatonin implantation can protect sperm from heat stress by optimizing the blood and semen microenvironment.

研究领域: 动物繁殖学 · 内分泌学 · 生殖生物学 · 生物化学 · 应激生理学
✓ 已验证 DOI PubMed 高影响因子
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Animal Nutrition · 2024 · IF 6.1

Maternal resveratrol improves the intestinal health and weight gain of suckling piglets under high summer temperatures: The involvement of exosome-derived microRNAs and immunoglobin in colostrum

Changming Hong,Yujian Huang,Guan Yang,Xiaolu Wen,Li Wang,Xuefen Yang,Kaiguo Gao,Zongyong Jiang,Hao Xiao

Previous studies have shown that maternal resveratrol improved growth performance and altered the microbial composition of suckling piglets under hot summer conditions. However, it remains unclear how maternal resveratrol improves growth performance of suckling piglets under high summer temperatures. A total of 20 sows (landrace × large white; three parity) were randomly assigned to 2 groups (with or without 300 mg/kg resveratrol) from d 75 of gestation to d 21 of lactation in a high temperature environment (from 27 to 30 °C). The results showed that maternal resveratrol supplementation increased total daily weight gain of piglets under hot summer conditions, which is consistent with previous studies. Furthermore, we found that maternal resveratrol improved the intestinal morphology and intestinal epithelial proliferation in suckling piglets. Dietary resveratrol supplementation affected the characteristics of exosome-derived microRNAs (miRNAs) in sow colostrum, as well as the genes targeted by differentially produced miRNAs. MiRNAs are concentrated in the tight junction pathway. As a result, the expression of intestinal tight junction proteins was increased in suckling piglets ( P < 0.05). Notably, maternal resveratrol increased the intestinal secretory immunoglobulin A (sIgA) levels of suckling piglets via colostrum immunoglobins ( P < 0.05), which could increase the abundance of beneficial microbiota to further increase the concentration of short chain fatty acids (SCFA) in suckling piglets' intestine ( P < 0.05). Finally, our correlation analysis further demonstrated the positive associations between significantly differential intestinal microbiota, intestinal sIgA production and SCFA concentrations, as well as the positive relation between total daily weight gain and intestinal health of suckling piglets. Taken together, our findings suggested that maternal resveratrol could promote intestinal health to improve piglet growth under high summer temperatures, which might be associated with the immunoglobin and exosome-derived miRNAs in sows’ colostrum.

CAS / MDL: 501-36-0
研究领域: 动物科学 · 营养学 · 微生物学 · 分子生物学
✓ 已验证 产品关联 DOI PubMed 高影响因子
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Food Chemistry-X · 2024 · IF 6.5

Sensory-directed flavor analysis reveals the improvement in aroma quality of summer green tea by osmanthus scenting

Yujie Wang,Guojian Deng,Lunfang Huang,Jingming Ning

Flower scenting is an effective way to enhance the aroma of green tea (GT), including those osmanthus scented green tea (OSGT). However, the mechanism of aroma enhancement by scenting is still unclear. Here, the volatiles of GT, OSGT, and osmanthus were detected by GC–MS. The total volatile content of OSGT was significantly increased compared to GT, with the flowery and coconut aromas enhanced. Furthermore, 17 of 139 volatiles were responsible for the enhancement by GC–olfactometry and their absolute odor activity values (OAVs). Aroma recombination, omission and addition experiments showed that dihydro- β -ionone, (E) - β -ionone, (E, E) -2,4-heptadienal, geraniol, linalool, α -ionone, and γ -decalactone were the key aroma volatiles with flowery or coconut aromas. Additionally, the dynamics of the key volatiles (OAVs >1) from different scenting durations were analyzed, proving that the optimal duration was 6–12 h. This study provides new insight into the mechanism of aroma formation during OSGT production.

研究领域: 食品科学 · 农业化学 · 分析化学
✓ 已验证 产品关联 DOI PubMed 高影响因子
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