INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2015
·
IF 2.858
Chao Li,Qiang Huang,Xiong Fu,Xiu-Jie Yue,Rui Hai Liu,Li-Jun You
Water-soluble polysaccharides from Prunella vulgaris Linn ( P. vulgaris ) were fractionated using DEAE-Sepharose fast-flow column to obtain several eluents of water (PV-P1), 0.1 M NaCl (PV-P2) and 0.2 M NaCl (PV-P3). Structural analyses showed that PV-P1 had a higher molecular weight and degree of branching as compared to PV-P2 and PV-P3. Tertiary structure analyses indicated that PV-P1, PV-P2 and PV-P3 did not have triple-helical conformation. PV-P2 and PV-P3 showed stronger antioxidant activities than PV-P1, as measured radical scavenging capacities. PV-P1 showed stronger immunomodulatory activities than PV-P2 and PV-P3 in term of stimulation of the production of pro-inflammatory cytokines, including nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in murine macrophage RAW 264.7 cells. PV-P1, PV-P2 and PV-P3 did not exhibit cytotoxicities against RAW 264.7 at the concentrations tested. These results suggest that P. vulgaris polysaccharides could be explored as potential antioxidant and immunomodulatory agents for the complementary medicine or functional foods.
研究领域:
药理学
·
免疫学
·
植物学
✓ 已验证
DOI
PubMed
高影响因子
FITOTERAPIA
·
2025
·
IF 2.6
Taoli Sun,Wei Yuan,Xinru Li,BoHou Xia,Jianhua Huang,Meihong Piao,Wen Chen,Qinhui Tuo
Atherosclerosis (AS), a leading cause of cardiovascular diseases, remains a major challenge due to the lack of effective preventive and therapeutic strategies. This study investigated the anti-atherosclerotic effects and underlying mechanisms of Prunella vulgaris polyphenols (PVPs). PVPs were found to significantly reduce lipid deposition in the aorta and enhance plaque stability by increasing fibrous cap thickness and collagen content, while simultaneously reducing MMP-9 and CD68 protein expressions. Moreover, PVPs alleviated hepatic lipid accumulation by upregulating ABCA1 and downregulating SREBP1 protein expressions. The PI3K-Akt pathway was identified as a key mechanism underlying the therapeutic effects of PVPs, with STAT3 predicted as a critical target involved in lipid metabolism. These predictions were further validated experimentally, demonstrating that PVPs effectively inhibited STAT3 activation while enhancing the PI3K-Akt pathway. Taken together, these findings reveal that PVPs exert anti-atherosclerotic effects by modulating lipid metabolism through the inhibition of STAT3 and activation of the PI3K-Akt pathway.
研究领域:
药理学
·
生物化学
·
分子生物学
·
心血管医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FITOTERAPIA
·
2024
·
IF 2.5
He Sun,Jia Lou,Bo-Xue Chen,Jia-Qi Huang,Qi-Long Wang,Shao-Fei Song,Zi-Yue Jia,Rong Miao,Shi-Yu Wang,Xue Li,Wen-Zhi Yang
Prunella vulgaris (PV) is a medicine and food homologous plant, but its quality evaluation seldom relies on the polysaccharides (PVPs). In this work, we established the multi-level fingerprinting and in vitro anti-inflammatory evaluation approaches to characterize and compare the polysaccharides of P. vulgaris collected from the major production regions in China. PVPs prepared from 22 batches of samples gave the content variation of 5.76–24.524 mg/g, but displayed high similarity in the molecular weight distribution. Hydrolyzed oligosaccharides with degrees of polymerization 2–14 were characterized with different numbers of pentose and hexose by HILIC-MS. The tested 22 batches of oligosaccharides exhibited visible differences in peak abundance, which failed to corelate to their production regions. All the PVPs contained Gal, Xyl, and Ara, as the main monosaccharides . Eleven batches among the tested PVPs showed the significant inhibitory effects on NO production on LPS-induced RAW264.7 cells at 10 μg/mL, but the exerted efficacy did not exhibit correlation with the production regions. Conclusively, we, for the first time, investigated the chemical features of PVPs at three levels, and assessed the chemical and anti-inflammatory variations among the different regions of P. vulgaris samples.
研究领域:
生药学
·
分析化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2025
·
IF 5.4
Yuanle Song,Zhi Zhou,Xinke Li,Xiujuan Shi,Hang Ma,Hong Chen,Chao Wu,Chen Zhang,Zeper Abliz
Ethnopharmacological relevance Isodon rubescens (Hemsl.) Hara, a traditional Chinese medicinal herb, demonstrates tumor inhibiting properties and therapeutic effecacy in treating esophageal squamous cell carcinoma (ESCC). However, its mechanism of action and the differential effects on varying ESCC differentiation grades remain unclear, despite the direct correlation between pathological grade and patient prognosis. Aim of the study To elucidate the effect heterogeneity of I. rubescens aqueous extracts on well-differentiated and poorly differentiated ESCC cell lines at metabolic level. Materials and methods A fusion strategy of network pharmacology combining targeted metabolomics was developed, focusing on key metabolic pathways through a “chemical components – targets – ESCC” network. Then, a targeted analysis method utilizing ultra-high performance liquid chromatography-multiple reaction monitoring tandem mass spectrometry (UPLC-MRM MS/MS) technology was established for core metabolites. The metabolic regulatory patterns of I. rubescens on two ESCC cell lines were determined through cytological experiments and targeted metabolomics analysis, with regulatory target enzymes verified through ELISA experiments. Molecular docking technology identified effective components acting on the regulatory enzymes. Results The primary regulatory pathways of I. rubescens in ESCC treatment encompassed central carbon metabolism, pyruvate metabolism, choline metabolism and retinol metabolism. A targeted metabolomics method analyzing 74 core metabolites in these pathways was established that had high accuracy and precision. The study confirmed heterogeneous effects of I. rubescens on well-differentiated KYSE-30 and poorly differentiated KYSE-150 cell lines. Citrate synthase (CS) expression varied significantly different between the two cell lines, showing upregulation after I. rubescens administration, albeit with varying degree. Molecular docking analysis revealed strong binding affinity between CS and four compounds: oridonin, quercetin, isorhamnetin, and ponicidin. Conclusions I. rubescens exhibits heterogeneous metabolic regulation in ESCC at different differentiation levels. CS was identified and confirmed as a crucial regulatory target, with oridonin, quercetin, isorhamnetin, and ponicidin serving as the primary active components.
研究领域:
药理学
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肿瘤学
·
代谢组学
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生物信息学
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民族药理学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2014
·Vol. 240
·期 1
·49-60
·
IF 1.387
Li Chao,Fu Xiong,Huang Qiang,Luo Faxing,You Lijun
Response surface methodology was used to optimize the ultrasonic extraction of Prunella vulgaris polysaccharides (PVP). When the quality of P. vulgaris was 4.0 g, the optimal extraction conditions were ultrasonic power 210 W, time 50 min, and temperature 70 °C. Using this condition, the experimental yield was agreed closely with the predicted value. Compared with conventional extraction, ultrasonic extraction showed a higher polysaccharide yield. The molecular weights were determined by high-performance gel permeation chromatography. Gas chromatography analysis suggested that PVP comprised of rhamnose, arabinose, xylose, mannose, glucose, and galactose with molar percentages of 2.8, 28.2, 38.5, 11.0, 3.0, and 16.5 %, respectively. The glycosidic linkage analysis showed that PVP contained 1→ and 1→6 glycosidic linkages (48.0 %), 1→2 and 1→4 glycosidic linkages (8.1 %), and 1→3 glycosidic linkages (43.9 %). PVP showed good antioxidant activities by the methods of DPPH radical scavenging assay, oxygen radical absorbance capacity assay, and cellular antioxidant activity assay. The antiproliferative activities of PVP (2.0 mg/mL) on the growth of human hepatoma HepG2, gastric carcinoma SGC 901, and human breast cancer MCF-7 cells were 51.2, 35.2, and 31.8 %, respectively. PVP did not exhibit cytotoxicity against normal liver L02 cells within the tested concentrations. These results demonstrated that polysaccharides isolated from P. vulgaris may be helpful to develop potential antioxidant and antitumor agents for food and pharmaceutical industries.
研究领域:
药理学
·
生物化学
·
食品科学
·
医学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）