Plant Reproduction
·
2021
·Vol. 34
·期 2
·91-101
·
IF 3.767
Jia Xin-Lei,Xue Jing-Shi,Zhang Fang,Yao Chi,Shen Shi-Yi,Sui Chang-Xu,Peng Yu-Jia,Xu Qin-Lin,Feng Yi-Feng,Hu Wen-Jing,Xu Ping,Yang Zhong-Nan
The pollen coat, which forms on the pollen surface, consists of a lipid–protein matrix. It protects pollen from desiccation and is involved in adhesion, pollen–stigma recognition, and pollen hydration during interactions with the stigma. The classical methods used for pollen coat observation are scanning and transmission electron microscopy. In this work, we screened a collection of fluorescence dyes and identified two fluorescent brighteners FB-52 and FB-184. When they were used together with the exine-specific dye, Basic fuchsin, the pollen coat and the exine structures could be clearly visualized in the pollen of Brassica napus . This co-staining method was applied successfully in staining pollen from Fraxinus chinensis , Calystegia hederacea , and Petunia hybrida . Using this method, small pollen coat-containing cavities were detected in the outer pollen wall layer of Oryza sativa and Zea mays . We further showed these dyes are compatible with fluorescent protein markers. In the Arabidopsis thaliana transgenic line of GFP-tagged pollen coat protein GRP19, GRP19-GFP was observed to form particles at the periphery of pollen coat . This simple staining method is expected to be widely used for the studies of the palynology as well as the pollen–stigma interaction.
研究领域:
植物科学
·
孢粉学
·
分子生物学
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DOI
PubMed
高影响因子
ChemNanoMat
·
2021
·Vol. 7
·期 4
·483-487
·
IF 3.154
Sihao Pan,Jiaoyu Ren,Enhui Ma,Ke Wang,Sicheng Yang,Hong Wang
Graphical Dual-propelled sporopollenin-exine-capsule (SEC) micromotors loaded with horseradish peroxidase (HRP) have been developed towards on-site degradation of organic pollutants. Near-infrared (NIR) light is employed to trigger the rupture of the magnetic polydopamine (PDA) outer coating and release the platinum-coated SECs loaded with HRP enzyme. Towards on-site degradation of organic pollutants, dual-propelled sporopollenin-exine-capsule (SEC) micromotors loaded with horseradish peroxidase (HRP) have been developed. The magnetic propulsion of SEC micromotors enables them to follow predesignated paths to arrive the pollution site, while the chemical propulsion on the site accelerates the degradation process taking advantage of the fluid mixing effect induced by their movement and bubble generation. Near-infrared (NIR) light is employed to trigger the rupture of the magnetic polydopamine (PDA) outer coating and release the platinum-coated SECs loaded with HRP enzyme. The dual propulsion system combined with the biocompatible nature of the components paves the way for on-site bioremediation of organic pollutants.
研究领域:
环境科学
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
MATERIALS & DESIGN
·
2021
·
IF 7.991
Yao Yang,Qianwen Zhang,Junwen Zhang,Anqi Chen,Yanxin Chen,Shengyu Li,Mingde Ye,Xuan Xuan,Xiaokun Li,Huacheng He,Jiang Wu
Herein, we discovered that the sunflower sporopollenin exine capsule (SEC) is an effective photothermal agent. In vitro study finds that the spikes on the surface of the sunflower SEC and its structural integrity are the two key factors to determine the photothermal effect. In vivo melanoma mouse model proves that the sunflower SEC can effectively inhibit tumor growth by inducing cell apoptosis and reducing cell proliferation through the near-infra red light triggered photothermal therapy. The in vivo biosafety of the SEC is also validated. In consideration of its safety, uniform size distribution and abundant availability, the sunflower SEC will be a promising photothermal agent candidate for clinical translational application.
研究领域:
生物医学工程
·
癌症研究
·
材料科学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Hang Jiang,Hengxing Yu,Xin Guan,Weijie Jiang,Yunxing Li,Wei Liu,Cheng Yang,Jianzhong Jiang,Bernard P. Binks,To Ngai
The preparation of monodisperse Pickering emulsions currently can only be achieved using microfluidics or membrane emulsification, limiting its widespread applications in many fields. Here, by simply introducing naturally occurring pollen grains during mechanical emulsification, it becomes extremely feasible to fabricate uniform Pickering emulsions. The process of shearing and turbulence leads to a constant reduction in the size of the droplets of the dispersed phase. The robust exine of the pollen grains in the continuous phase enables them to retain their structural integrity during emulsification, resulting in the formation of a single pollen-in-water-in-oil structure. Meanwhile, the intrinsic monodispersity of the pollen grains allows for the resulting emulsions to be similarly uniform, and they can be easily collected through centrifugation. As a result, this novel methodology achieves the effective formulation of monodisperse Pickering emulsions in an unprecedented way. Furthermore, the utilization of sporopollenin exine capsules (SECs) as a replacement for pollen, in conjunction with sol–gel interfacial engineering, enables the attainment of “yolk-shell” dual-shell microcapsules. This approach not only effectively addresses the issue of cargo leakage in SECs but also imparts exceptional uniformity and stability to the microcapsules.
研究领域:
材料科学
·
化学工程
·
胶体科学
·
生物技术
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产品关联
DOI
高影响因子
ACS Applied Bio Materials
·
2021
·
IF 0
Ziyu Deng,Shishuai Wang,Yaqiong Pei,Bin Zhou,Jing Li,Xinyao Hou,Bin Li,Hongshan Liang
There are multiple obstacles for the storage and digestion of orally administered bioactive macromolecules. This study developed a low-cost and sustained-release delivery system (sporopollenin exine capsules with zein/tannic acid modification) of proteins with excellent storage stability, and at the same time provided insights into the sustained-release mechanism through exploring the interaction between zein and tannic acid (TA). β-Galactosidase (β-Gal) was utilized as a model protein and loaded into sporopollenin exine capsules (SECs), which were then coated with the zein/TA system. Under the optimized zein/TA conditions, the zein/TA system showed better performance than the zein alone system in the sustained release of β-Gal, with the residual activity of about 70.26% after 24 h of simulated digestion. Evaluation of the storage stability demonstrated a β-Gal residual activity of nearly 90% for 28 days at 25 °C. Additionally, FTIR analysis demonstrated that the stability of the zein/TA system depends on both hydrogen bonding and certain covalent bonding through the Schiff-base reaction, and the sustained release is regulated by the bonding strength.
研究领域:
药学
·
材料科学
·
生物技术
·
化学
✓ 已验证
产品关联
DOI
PubMed
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