Frontiers of Materials Science
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2024
·Vol. 18
·期 1
·1-11
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IF 2.5
Lan Weiwei,Wang Mingbo,Lv Zhenjun,Li Jun,Chen Fuying,Liang Ziwei,Huang Di,Wei Xiaochun,Chen Weiyi
In the field of bone defect repair, critical requirements for favorable cytocompatibility and optimal mechanical properties have propelled research efforts towards the development of composite materials. In this study, carbon nanotubes/polylactic acid/hydroxyapatite (CNTs/PLA/HA) scaffolds with different contents (0.5, 1, 1.5 and 2 wt.%) of CNTs were prepared by the thermally induced phase separation (TIPS) method. The results revealed that the composite scaffolds had uniform pores with high porosities over 68% and high through performances. The addition of CNTs significantly enhanced the mechanical properties of resulted PLA/HA, in which the 1.5 wt.% CNTs/PLA/HA composite scaffold demonstrated the optimum mechanical behaviors with the bending elastic modulus of (868.5 ± 12.34) MPa, the tensile elastic modulus of (209.51 ± 12.73) MPa, and the tensile strength of (3.26 ± 0.61) MPa. Furthermore, L929 cells on the 1.5 wt.% CNTs/PLA/HA scaffold displayed good spreading performance and favorable cytocompatibility. Therefore, it is expected that the 1.5 wt.% CNTs/PLA/HA scaffold has potential applications in bone tissue engineering.
研究领域:
生物材料学
·
医学工程
✓ 已验证
DOI
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 64
·39241-39250
·
IF 3.119
Shuifeng Liu,Da Huang,Yang Hu,Jiancheng Zhang,Bairui Chen,Hongwu Zhang,Xianming Dong,Rongbiao Tong,Yiheng Li,Wuyi Zhou
Biocompatible porous scaffolds with adjustable pore structures, appropriate mechanical properties and drug loading properties are important components of bone tissue engineering. In this work, biocompatible sodium alginate (SA)/collagen (Col) multiscale porous scaffolds containing poly(ε-caprolactone) microspheres (Ms-PCL) have been facilely fabricated based on 3D extrusion printing of the pre-crosslinked composite hydrogels. The prepared composite hydrogels can be 3D extrusion printed into porous scaffolds with different designed shapes and adjustable pore structures. The hydroxyapatite (HAP) nanoparticles have been added into the SA/Col hydrogels to achieve stress dispersion and form double crosslinking networks. SA-Ca2+ crosslinking networks and Col–genipin (GP) crosslinking networks have been constructed to improve the mechanical properties of the scaffolds (about 2557 kPa of compressive stress at 70% strain), and reduce the swelling rate and degradation rate of SA/Col scaffolds. Moreover, the SA/Col hydrogels contain hydrophobic antibacterial drug enrofloxacin loaded Ms-PCL, and in vitro drug release research shows a sustained-release function of porous scaffolds, indicating the potential application of SA/Col porous scaffolds as drug carriers. In addition, the antibacterial experiments show that the composite scaffolds display a distinguished and long-term antibacterial activity against Escherichia coli and Staphylococcus aureus. Furthermore, mouse bone mesenchymal stem cells (mBMSCs) are seeded on the SA/Col composite scaffolds, and an in vitro biocompatibility experiment shows that the mBMSCs can adhere well on the composite scaffolds, which indicate that the fabricated composite scaffolds are biocompatible. In short, all of the above results suggest that the biocompatible SA/Col composite porous scaffolds have enormous application and potential in bone tissue engineering.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
DOI
高影响因子
Journal of Orthopaedic Translation
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2017
·
IF 0
Ling Li,Jing Long,Long Li,Huijuan Cao,Tingting Tang,Xinghua Xi,Ling Qin,Yuxiao Lai,Xinluan Wang
Summary Background/Objective A novel porous scaffold poly (lactide- co -glycolide) and tricalcium phosphate (PLGA/TCP) was developed by three-dimensional printing technology for bone defect repair. As a Class 2 solvent with less severe toxicity, content of residual 1,4-dioxane in this newly developed scaffold should be rigorously controlled when it is translated to clinical use. In this study, a headspace gas chromatography-mass spectrometric (HS-GC-MS) method and related testing protocol were developed for quantitative determination of 1,4-dioxane in the PLGA/TCP composite scaffolds. Methods Matrix effect analysis was used to optimise the pretreatment method of the scaffolds. Then, the procedure for testing 1,4-dioxane using HS-GC-MS was set up. The accuracy, precision, and robustness of this newly developed quantitative method were also validated before quantification of 1,4-dioxane in the scaffolds with different drying procedures. Results Dimethyl formamide (DMF) was the optimal solvent for dissolving scaffolds for GC-MS with proper sensitivity and without matrix effect. Then, the optimised procedure was determined as: the scaffolds were dissolved in DMF and kept at 90°C for 40 minutes, separated on a HP-5MS column, and detected by mass spectroscopy. Recovery experiments gave 97.9–100.7% recovery for 1,4-dioxane. The linear range for 1,4-dioxane was determined as 1–40 ppm with linear correlation coefficient ≥ 0.9999. Intraday and interday precision was determined as being within relative standard deviation of below 0.68%. The passable drying procedure was related to lyophilising (−50°C, 50 Pa) the scaffolds for 2 days and drying in vacuum (50 Pa) for 7 days. Conclusion This is the first quantitative method established to test 1,4-dixoane in a novel scaffold. This method was validated with good accuracy and reproducibility, and met the methodological requirements of the Guideline 9101 documented in the Chinese Pharmacopoeia 2015 Edition. The translational potential of this article This quantitative method for determination of residual 1,4-dioxane in the novel scaffolds is a key technical method during its translation into clinical use because this method is an important and indispensable file in the enterprise standard when the porous scaffold is registered as a Class III implanted medical device for bone defect repair, which is used to guarantee the safety of the scaffolds. It is also applied to optimise the drying process of scaffolds and to monitor the quality of scaffolds in the industrialisation process. Further, this method provides references for other solvents quantitative determination in porous scaffolds or materials.
研究领域:
生物材料学
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分析化学
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医学工程
✓ 已验证
DOI
COLLOIDS AND SURFACES B-BIOINTERFACES
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2013
·
IF 3.554
Youjia Zhu,Ying Wan,Jun Zhang,Dengke Yin,Wenze Cheng
Chitosan-polycaprolactone (CH-PCL) copolymers with various PCL percentages less than 45 wt% were synthesized. Different CH-PCLs were respectively blended with Type-II collagen at prescribed ratios to fabricate a type of layered porous scaffolds with some biomimetic features while using sodium tripolyphosphate as a crosslinker. The compositions of different layers inside scaffolds were designed in a way so that from the top layer to the bottom layer collagen content changed in a degressive trend contrary to that of chitosan. A combinatorial processing technique involving adjustable temperature gradients, collimated photothermal heating and freeze-drying was used to construct desired microstructures of scaffolds. The resultant scaffolds had highly interconnected porous layers with a layer thickness of around 1 mm and porous interface zones without visual clefts. Results obtained from SEM observations and measurements of pore parameters and swelling properties as well as mechanical examinations confirmed that graded average pore-size and porosity, gradient swelling index and oriented compressive modulus for certain scaffolds were synchronously achieved. In addition, certain evaluations of cell-scaffold constructs indicated that the achieved scaffolds were able to well support the growth of seeded chondrocytes. The optimized collagen/CH-PCL scaffolds are partially similar to articular cartilage extracellular matrix in composition, porous microarchitecture, water content and compressive mechanical properties, suggesting that they have promising potential for applications in articular cartilage repair.
研究领域:
生物材料学
·
组织工程学
·
再生医学
✓ 已验证
DOI
高影响因子
Journal of Macromolecular Science Part B-Physics
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2020
·
IF 0.911
Ping Chen,Zhihua Zhou,Wenjuan Liu,Yanmin Zhao,Tianlong Huang,Xiaofei Li,Jianglong Duan,Jianjun Fang
The technique of thermally induced phase separation (TIPS) is favorable for the fabrication of a porous scaffold due to a number of advantages. In this work the poly(L-lactide-co-glycolide-co-ε-caprolactone) (PLLGC) terpolymer was synthesized by melt copolymerization and porous scaffolds thereof from its solution in 1,4-dioxane were fabricated by using the TIPS method. The effects of fabrication parameters, including polymer concentration and freezing temperature, on the morphology, pore size and mechanical properties were studied. The results showed that the average pore size of the PLLGC porous scaffold increased with a decrease in PLLGC concentration and the pore size resulting from freezing at 4 °C (about 20–100 μm) was significantly larger than for other samples (20–50 μm) frozen at lower temperatures. The porosity of the scaffolds decreased with increasing PLLGC concentration or decreasing freezing temperature. On the other hand, the compressive strength of the scaffolds increased with the increase of PLLGC concentration or the decrease of freezing temperature, as would be expected. The present results can be applied in design to control the processing parameters of TIPS for a scaffold with desired pore morphology.
研究领域:
生物材料
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组织工程
·
高分子科学
·
材料表征
✓ 已验证
DOI
高影响因子
Journal of Energy Storage
·
2022
·
IF 8.907
Liping Zhen,Genping Meng,Yongjie Yang,Mo Zhang,Bo Zhou,Huazhi Wang
Tuning the pore structure of scaffold materials is an effective avenue to preclude the leakage issue of phase change materials (PCMs) and optimize the thermal energy storage capacity of composites for practical applications. Herein, we precisely tuned the pore configuration of nano-aluminosilicate aggerates (n-ASA) by desilication-rearrangement mechanism to systematically investigate the influence of pore size on the energy-storage performance of polyethylene glycol (PEG)-based composites. n-ASA scaffolds with the mean pore size from 11.2 to 27.0, 51.7 and 111.0 nm were synthesized respectively, and the corresponding PCM composites n-ASA*n@PEG were prepared successfully. Among them, n-ASA*1@PEG exhibited the highest heat storage performance on account of the moderate pore size of 27.0 nm and the highest pore volume in all the prepared scaffolds. The load of PEG in the composite reached 81.0 wt%, and the corresponding melting and solidifying enthalpies were 139.8 J/g and 123.8 J/g, respectively. Furthermore, the mechanism of desilication-rearrangement is ideally suited for tailoring the pore size of silica matrixes, and the modulation of pore characteristics would be a feasible protocol to construct efficient energy-storage systems.
研究领域:
材料科学
·
能源化学
·
纳米材料
·
热物理
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功能材料
·
储能技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）