Natural Product Communications
·
2020
·
IF 0.468
Weida Zhang,Shaobo Cheng,Xiaona Zhai,Junshe Sun,Xuefang Hu,Haisheng Pei,Guogang Chen
Deep eutectic solvents (DESs) were proposed for the extraction of polysaccharides from Poria cocos (PCPs). Six types of DESs were prepared, and the DES composed of choline chloride and oxalic acid was proved to be suitable. Based on the results of single-factor test, the Box-Behnken experimental design with response surface methodology was carried out, giving the optimal extraction conditions including mole ratio of 1:2 (choline chloride:oxalic acid) and extraction 15 minutes at 100°C. Under the optimal extraction conditions, the extraction yield (46.24% ± 0.13%) was 8.6 times higher than that of hot water. The reusability of DES was demonstrated by a 6-run test, and an extraction yield of PCP was 38.40% ± 0.23% after reusing for 6 times without adding any additional chemicals. Moreover, molecular weight distributions of the resulting PCP were analyzed, and then mainly distributed in the range of 753 to 3578 g/mol. Therefore, DESs were proved to be an excellent extraction solvent alternative to the extraction of PCP.
研究领域:
植物化学
·
食品科学
✓ 已验证
DOI
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Jibao Deng,Hefei Wang,Haisheng Zhan,Chenxi Wu,Yi Huang,Bing Yang,Ahmed Mosa,Wanting Ling
The capability of laccase to oxidate a broad range of polyphenols and aromatic substrates in vitro offers a new technological option for the remediation of polycyclic aromatic hydrocarbon (PAH) pollution with high cytotoxicity. However, laccase application in the remediation of PAH-contaminated sites mainly suffers from a low oxidation rate and high cost because of the difficulty in its recovery. In this study, laccases were immobilized on magnetic Fe 3 O 4 particles coated with chitosan (Fe 3 O 4 @SiO 2 -chitosan) to improve the operational stability and reusability in the treatment of PAH pollution. The enzyme fixation capacity reached 158 mg g −1 , and 79.1% of free laccase activities were reserved under the optimum immobilized condition of 4% glutaraldehyde, 1.0 mg mL −1 laccase, 2 h covalent bonding time, and 6 h fixation time. The degradation efficiencies of anthracene (ANT) and benzo[a]pyrene (B(a)P) by Fe 3 O 4 @SiO 2 -chitosan immobilized laccase in 48 h were 81.9% and 69.2%, respectively. Furthermore, it is very easy to magnetically recover the immobilized laccase from reaction systems and reuse it in a new batch. The relative activities of immobilized laccase were over 50% for the degradation of ANT and B(a)P in three catalytic runs, reaching the goal of substantially reducing cost in practice. According to the results from quantum calculations and mass spectrum analyses, the degradation products of ANT and B(a)P by laccase were anthraquinone and B(a)P-dione, respectively. The findings from this study provide valuable insight in promoting the application of immobilized laccase technology in the remediation of PAH contamination.
研究领域:
环境科学
·
生物技术
·
化学工程
✓ 已验证
DOI
高影响因子
Chemical and Biological Technologies in Agriculture
·
2024
·Vol. 11
·期 1
·1-13
·
IF 5.2
Qu Yunhe,Zhang Pingping,Zhao Boya,Xu Jing,Shi Dongfang
The total polysaccharides extracted from Laetiporus sulphureus fruiting bodies by ultrasound-assisted complex enzyme method were separated by freeze–thaw combined with fehling reagent to prepare purified polysaccharide (LSPS1). The results of monosaccharide composition and molecular weight distribution demonstrated that LSPS1 contained galactose (51.83%), mannose (26.89%), fucose (16.13%) and glucose (5.15%), and the molecular weight was around 17.3 kDa. Methylation analysis indicated that the backbone of LSPS1 consisted of 1,6-Gal p residues, branched at O-2 of Gal p with t-Man p , t-Fuc p and t-Glc p residues as side chains. In addition, LSPS1 was used for the synthesis of SeNPs based on the redox system of sodium selenite and ascorbic acid. The L. sulphureus polysaccharide selenium nanoparticles (LSPS1–SeNPs) were characterized by UV, FT-IR, ICP-MS, EDX, DLS and SEM, inferring that LSPS1–SeNPs were stabilized with spherical and granular surface structure as well as a mean particle size of 96.72 ± 1.12 nm. Both of LSPS1 and LSPS1–SeNPs possessed strong antioxidant and hypoglycemic activities while the effects of LSPS1–SeNPs were superior to LSPS1. The findings provided valuable structural information for L. sulphureus polysaccharide, and will promote the application of SeNPs by L. sulphureus polysaccharide in potential antioxidant agent and hypoglycemic candidate. Graphical
研究领域:
生物化学
·
药理学
·
纳米医学
✓ 已验证
产品关联
DOI
高影响因子
ACS Omega
·
2021
·
IF 3.512
Zili Song,Ruilin Zhao,Hongjiao Zhang,Penglin Wei,Landa Qi,Guocan Chen,Wen-Bing Yin,Wei Li
Edible mushrooms are important nutraceutical sources of foods and drugs, which can produce various nutritional ingredients including all essential amino acids. The method of rapid screening for the strains producing specific functional components is very indispensable. Homocitrate synthase is one of the key enzymes in the α-aminoadipate pathway for lysine biosynthesis and has preferable sequence conservation in Agaricales. Based on the blast of homocitrate synthase homologous genes of strains of Agaricales, we achieved combinations of degenerate primers as molecular markers to rapidly screen the lysine-producing edible mushrooms. The experimental results revealed that the consistency between PCR amplification and HPLC analysis attained 82 and 75% in strains of Agaricales and Polyporales, respectively. The finding showed that the molecular marker has higher universality for screening edible mushroom resources of Agaricales. This PCR-based approach shows excellent potential in evaluating and discriminating edible wild-grown mushrooms with high lysine content in Agaricales.
研究领域:
真菌学
·
分子生物学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Cheng Han,Yinping Zhang,Marc Redmile-Gordon,Huan Deng,Zhenggui Gu,Qiguo Zhao,Fang Wang
This study conducted the sorption and biodegradation of benzo[ a ]pyrene (BaP) by microbial biofilm communities developed on proxies for materials typically found in soils. The half-life of BaP was 4.7 and 2.3 weeks for biofilms on the inorganic carrier (BC INOR , montmorillonite) and on the organic carrier (BC OR , humic acid), respectively. In contrast, the half-life was 7.0 weeks for specialized planktonic cultures (PK). The exposure to BaP caused the development of lipid inclusion bodies inside the bacteria of the PK systems and biofilms of the BC INOR , but not on the biofilms of the BC OR system. Interestingly, the BC OR displayed not only the greatest BaP sorption capacity but also the greatest bacterial density and membrane integrity and the shortest bacteria-to-bacteria distances, which were consistent with the increased production of cell surface extracellular polymeric substances on the BC OR . Both carriers caused a noticeable shift in the bacterial genera during the biodegradation of the BaP. The BC INOR selected for Rhodococcus, Brucella, Chitinophaga , and Labrys , whereas the BC OR favored Rhodococcus and Dokdonella . It indicated that ultra-structure and BaP degradation within the organic carrier-attached biofilms differed from the inorganic ones, and suggested that the microstructural heterogeneity and microbial biodiversity from biofilms on the organic carrier promoted biodegradation.
研究领域:
环境微生物学
·
土壤学
·
污染物降解
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Mengyu Li,Yahan Bai,Wei Zhuang,Jinle Liu,Zhi Wang,Yuan Rao,Mengran Li,Hanjie Ying,Pingkai Ouyang
A novel magnetic 2D/2D heterogeneous structure MXene@NiFe-LDH@Fe 3 O 4 was prepared for immobilization of laccase. In this work, two-dimensional MXene nanosheets with abundant surface functional groups were heterogeneously assembled with layered double hydroxide (LDH) by in situ co-precipitation method, and magnetic nanoparticle Fe 3 O 4 with excellent biocompatibility and rapid separation of materials and substrates was introduced subsequently, and then silane coupling agent was coated on the surface of MXene@NiFe-LDH@Fe 3 O 4 . The functionalized MXene@NiFe-LDH@Fe 3 O 4 was employed as a carrier to immobilize laccase from Trametes-Versicolor . The enzyme loading of the nanocomposite material is as high as 167.9 mg/g. Compared with free enzymes, the immobilized laccase showed a notable improvement in stability in a wider range of pHs (2.0–8.0), temperatures (25–60 °C), and organic solvent concentration (1–5 M). The reusability study suggested that after 7 cycles of repeated catalysis, the degradation efficiency could reach 55.5% for 2,4-dichlorophenol, 92.1% for bisphenol A and70.9% for pyrocatechol. The results provide a new carrier preparation strategy for the efficient immobilization of laccase.
研究领域:
环境科学
·
纳米技术
·
生物催化
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
iMeta
·
2024
·
IF 23.7
Shi Zhong,Yu-Qing Sun,Jin-Xi Huo,Wen-Yi Xu,Ya-Nan Yang,Jun-Bo Yang,Wei-Jie Wu,Yong-Xin Liu,Chong-Ming Wu,You-Gui Li
Inflammatory bowel disease (IBD) is a significant global health concern. The gut microbiota plays an essential role in the onset and development of IBD. Sanghuangporus (SH), a traditional Chinese medicinal mushroom, has excellent anti-inflammatory effects and is effective at modulating the gut microbiota. Despite these attributes, the specific anticolitic effects of SH and the mechanisms through which the gut microbiota mediates its benefits remain unclear. Herein, we demonstrated that polyphenol-rich extract from SH effectively alleviated the pathological symptoms of dextran sodium sulfate (DSS)-induced colitis in mice by modulating the gut microbiota. Treatment with SH distinctly enriched Alistipes , especially Alistipes onderdonkii , and its metabolite 5-hydroxyindole-3-acetic acid (5HIAA). Oral gavage of live A. onderdonkii or 5HIAA potently mitigated DSS-induced colitis in mice. Moreover, both 5HIAA and SH significantly activated the aromatic hydrocarbon receptor (AhR), and the administration of an AhR antagonist abrogated their protective effects against colitis. These results underscore the potent efficacy of SH in diminishing DSS-induced colitis through the promotion of A. onderdonkii and 5HIAA, ultimately activating AhR signaling. This study unveils potential avenues for developing therapeutic strategies for colitis based on the interplay between SH and the gut microbiota. Graphical Sanghuangporus is a medical mushroom with potent anticolitic effects. It modulates the gut microbiota and specifically enriches Alistipes onderdonkii , which can produce 5-hydroxyindole-3-acetic acid, thus activating the aryl hydrocarbon receptor pathway and ameliorating colitis. Highlights Sanghuangporus (SH) polyphenol extract potently alleviated symptoms of colon colitis in dextran sodium sulfate-induced colitis mice. The gut microbiota especially Alistipes onderdonkii mediates the anticolitic effect of SH. The SH-enriched microbial metabolite 5-hydroxyindole-3-acetic acid exhibited an anticolitic effect via the activation of the aryl hydrocarbon receptor. The “gut microbiota-metabolites-signaling pathway” provides an effective perspective for the study of complex Chinese medicine systems.
研究领域:
微生物学
·
免疫学
·
药理学
·
胃肠病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2019
·
IF 5.108
Kai Sun,Shunyao Li,Jialin Yu,Rui Gong,Youbin Si,Xiaohong Liu,Gang Chu
Laccase-mediated humification processes (L-MHPs) can be used to polymerize and transform phenolic pollutants in water. However, the mechanism on Cu 2+ impacts the self-polymerization of multi-purpose antimicrobial agent triclosan during L-MHPs is less understood. Here, the influence of divalent metal ions (DMIs) on Trametes versicolor laccase activity was investigated. Particularly, the performance of Cu 2+ -assisted laccase in polymerizing and transforming triclosan was systematically characterized. Compared with DMI-free, the activity of laccase was obviously accelerated with Cu 2+ present due to copper is a vital component of laccase catalytic center. It was found that Cu 2+ -assisted laccase was effective in transforming triclosan, and the enzymatic reaction kinetic constants increased from 0.28 to 0.73 h −1 as the Cu 2+ concentration increased (0–3.0 mM). Identification of intermediate products revealed that laccase oxidation predominantly generated triclosan dimers, trimers , and tetramers. The presence of Cu 2+ reinforced self-polymerization of triclosan via forming more triclosan oligomers relative to the Cu 2+ -free, which likely attributed to the enhancement of laccase activity and stability with Cu 2+ present in L-MHPs. A possible transformation mechanism was proposed as follows: Laccase initially catalyzed the oxidation of triclosan to generate phenoxy radical intermediates, which self-coupled to each other subsequently by radical-mediated C C and C O C covalent binding, forming oligomers and polymers. The growth inhibitory assays of freshwater microalgae ( Chlamydomonas reinhardtii and Scenedesmus obliquus ) demonstrated that the self-polymerized triclosan by L-MHPs had lower toxicity than the parent compound. These findings implied that Cu 2+ -assisted laccase was an effective strategy for rapidly self-polyreaction and detoxication of triclosan from Cu 2+ -triclosan combined polluted wastewater.
研究领域:
环境科学
·
生物技术
·
化学工程
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）