CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Chao Hua,Fengbo Yang,Xiaoli Jia,Yichi Lu,Xiaoxiao Li,Peng Zhao,Malcolm Xing,Guozhong Lyu
Infection and vascular dysfunction are two major reasons for delayed wound healing, especially in the scenario of multidrug-resistant bacterial has been a challenge for antibiotics usage. Probiotics are effective in fighting pathogens, while avoiding the destruction of skin microbiome. However, the viability of probiotics is a major obstacle in clinical application. As a non-protein proangiogenic drug, deferoxamine (DFO) has significant advantages over traditional growth factors in terms of cost, storage and transportation conditions, and stability. However, there is still a problem of short half-life that requires reliable drug delivery carriers to maximize its effect. Here, we developed multiple-chambered microgels using electrospray and microfluidics. Lactobacillus fermentum (LF) and DFO, respectively, for the management of multidrug-resistant Pseudomonas aeruginosa (MPA) and promotion of wound healing. We first tested five probiotics isolated from healthy people and found LF inhibiting the growth of MPA by lowering local pH and antimicrobial substances. The multi-chambered microgels effectively inhibited MPA by delivering probiotics, they can also protect probiotics from antibiotics. DFO promoted the angiogenesis of human umbilical venous endothelial cells. The microgel system had good biocompatibility and hemocompatibility. In a MPA infected wound, the microgel system effectively alleviated wound infection by 87.44%, improved angiogenesis by 29.32% and accelerated wound healing. This work suggests that multi-compartmented microgels loaded with probiotics and deferoxamine have great potential in wounds with multidrug-resistant bacteria.
研究领域:
生物医学工程
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微生物学
·
药剂学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Changyue Xu,Zhuo Chen,Chaoxia Wang,Kunlin Chen
By designing and preparing multifunctional materials exhibiting self-healing ability, problems related to their durability outdoors can be solved. This study, inspired by the self-healing mechanism of natural creatures, successfully prepared a dual self-healing multifunctional coating using temperature stimuli-responsive multicompartment microcapsules. Phase change materials (PCMs) were employed to load multicompartment microcapsules that were produced through Pickering emulsion polymerization by applying hydrophobic materials encapsulated by titanium dioxide (TiO2) nanocapsules as Pickering emulsifiers. The multifunctional coating produced using microcapsules and self-healing waterborne polyurethane (WPU) exhibited thermal insulation and antireflection properties, which was attributed to the application of PCMs and TiO2, and it also achieved remarkable superhydrophobicity. Moreover, this coating exhibited the intrinsic and superficial dual self-healing ability, which was attributed to the release of hydrophobic materials from microcapsules and the self-healing ability of WPU. This study can be referenced to guide the fabrication of high-performance self-healing materials, and it can contribute to the long-term use of multifunctional coatings.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
PubMed
高影响因子
BIOMACROMOLECULES
·
2022
·
IF 6.978
Qingli Qu,Weixia Cheng,Xiaoli Zhang,Aying Zhou,Yankang Deng,Miaomiao Zhu,Tianjiao Chu,Bella B. Manshian,Ranhua Xiong,Stefaan J. Soenen,Kevin Braeckmans,Stefaan C. De Smedt,Chaobo Huang
Inspired by the structure of eukaryotic cells, multicompartmental microcapsules have gained increasing attention. However, challenges remain in the fabrication of “all-aqueous” (i.e., oil-free) microcapsules composed of accurately adjustable hierarchical compartments. This study reports on multicompartmental microcapsules with an innovative architecture. While multicompartmental cores of the microcapsules were fabricated through gas shearing, a shell was applied on the cores through surface gelation of alginate. Different from traditional multicompartmental microcapsules, thus obtained microcapsules have well-segregated compartments while the universal nature of the surface-gelation method allows us to finely tune the shell thicknesses of the microcapsules. The microcapsules are highly stable and cytocompatible and allow repeated enzymatic cascade reactions, which might make them of interest for complex biocatalysis or for mimicking physiological processes.
研究领域:
生物材料学
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生物化学
·
仿生学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Wing-Fu Lai,Andrei S. Susha,Andrey L. Rogach
Multidrug therapy may yield higher therapeutic effects as compared to monotherapy, yet its wide application has been hampered by the limitations of conventional drug delivery systems, in which not only incompatible drugs cannot be co-delivered but also the release rates of individual co-delivered drugs cannot be tuned separately. Regarding these limitations, we adopt the microfluidic electrospray technology to fabricate alginate-based multicompartment microgel beads. By using cadmium–telluride (CdTe) quantum dots (QDs) and a quenching agent as a model pair, the beads are shown to effectively separate incompatible drugs during co-delivery, and significantly prolong the time of observable fluorescence emission from QDs co-delivered with a quenching agent. Moreover, the drug release rates from different compartments can be tuned using the polymer blending technique to achieve a variety of drug release patterns. This study is one of the first to adopt the microfluidic electrospray technology to generate microgel beads with such versatility for co-delivery of multiple drugs. Our results provide evidence for the promising potential of our beads to be further developed as a carrier for multidrug therapy and other applications that require co-administration of multiple bioactive agents.
研究领域:
生物医学工程
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药剂学
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材料科学
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DOI
PubMed
高影响因子
Biomicrofluidics
·
2013
·Vol. 7
·期 4
·
IF 3.385
Liu Zhou,Shum Ho Cheung
In this work, we demonstrate a robust and reliable approach to fabricate multi-compartment particles for cell co-culture studies. By taking advantage of the laminar flow within our microfluidic nozzle, multiple parallel streams of liquids flow towards the nozzle without significant mixing. Afterwards, the multiple parallel streams merge into a single stream, which is sprayed into air, forming monodisperse droplets under an electric field with a high field strength. The resultant multi-compartment droplets are subsequently cross-linked in a calcium chloride solution to form calcium alginate micro-particles with multiple compartments. Each compartment of the particles can be used for encapsulating different types of cells or biological cell factors. These hydrogel particles with cross-linked alginate chains show similarity in the physical and mechanical environment as the extracellular matrix of biological cells. Thus, the multi-compartment particles provide a promising platform for cell studies and co-culture of different cells. In our study, cells are encapsulated in the multi-compartment particles and the viability of cells is quantified using a fluorescence microscope after the cells are stained for a live/dead assay. The high cell viability after encapsulation indicates the cytocompatibility and feasibility of our technique. Our multi-compartment particles have great potential as a platform for studying cell-cell interactions as well as interactions of cells with extracellular factors.
研究领域:
生物工程
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
Environmental Technology & Innovation
·
2024
·
IF 6.7
Cheng Chang,Zitao Li,Saeed Rad,Lei Gan,Junfeng Dai,Asfandyar Shahab
The high demand for water treatment and environmental restoration via advanced oxidation process (AOP) has estimated the increased production of TiO 2 -based materials. Low cost, high transmittance, durability, and thermal stability, make the recycling low-iron crushed glass an excellent candidate for nano TiO 2 film coating via sol-gel process. Besides support selection, the reactor design plays a vital role in the final product's applicability and efficiency. In this work, small pieces (3–6 mm) of recycled low iron crushed glass were deep coated with multiple layers of nano TiO 2 film for photocatalytic degradation of selected contaminants in a new design packed-bed reactor. A multi-chamber packed-bed photocatalytic reactor was set up for efficient BOD degradation under UV light exposure. SEM, XRD, and BET analysis characterized coating characteristics, morphology, phase, and surface area. For film adherence and coating durability, SEM characteristics and durability of TiO 2 photocatalytic activity tests were performed after the second reutilization. Results revealed that the new reactor can achieve 45.81 up to 79.50% BOD 5 reduction after 24 to 48 h of circulation with constant operation, respectively. low iron crushed glass appeared to be a perfect support for TiO 2 film coating with a BET of 0.0742 m 2 /g after 9 cycles of coating. XRD results showed 95–97% anatase phase crystals with an average particle size of 12.97 nm with different rounds of annealing at 550 °C in muffle. Results underpinned that the technique seems promising to be applied on a large scale for pretreated industrial or pharmaceutical wastewater.
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2021
·
IF 13.273
Qingli Qu,Xiaoli Zhang,Hossein Ravanbakhsh,Guosheng Tang,Jian Zhang,Yankang Deng,Kevin Braeckmans,Stefaan C. De Smedt,Ranhua Xiong,Chaobo Huang
Core-shell multicompartmental microparticles have gained increasing attention as their architecture resembles the multicompartmental composition of eukaryotic cells. However, challenges remain in the fabrication of biocompatible multicompartmental microparticles at a sufficiently high scale. Herein, all-aqueous ‘mangosteen-like’ core–shell multicompartmental microparticles were created by an oil-free gas-shearing fabrication approach. Both the shell thickness and core size of thus obtained mangosteen-like microparticles can be easily finetuned. Mangosteen-like particles have well segregated (up to eight) compartments which are attractive to precisely control enzymatic cascade reactions. To illustrate the application potential, insulin releasing mangosteen-like microparticles (‘artificial pancreatic beta cells’) were developed.
研究领域:
生物工程
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材料科学
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生物化学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Wenzhe Zhang,Ying Wan,Chaoyi Xue,Zhengxing Chen,Ren Wang,Wei Feng,Yanan Li,Tao Wang
Multicompartmented nanostructures, which mirror the complexity of living cells, are promising for bio-related applications. However, controlling the spatial arrangement within natural biopolymer assemblies, like protein nanoparticles, is challenging. In our study, we reveal a facile method for compartmentalizing the interiors of protein nanoparticles by co-assembling rice proteins (RPs) and soy protein isolates (SPIs) through simultaneous folding. The assembly of these two protein types results in nanoparticles with a hydrophobic core to accommodate hydrophobic substances, such as eugenol, leading to its internalization. The protein–ligand (SPI-eugenol) interaction then triggers SPI gelation within the nanoparticle cores, forming distinct physical barriers hindering the complete merging of eugenol droplets. Subsequent dialysis of the nanoparticles containing eugenol against water facilitated the removal of eugenol. This step was crucial for forming multicompartmented nanoparticles (MCNPs) with adjustable interior complexities, including varying numbers of compartments and different hollowing rates related to various SPI:RP mass ratios. This allows us to endow MCNPs with adjustable barrier effects tailoring controllable responses of the encapsulated payload, vitamin D 3 (VD 3 ), under different physicochemical conditions. Our study demonstrates a straightforward method for constructing biomacromolecular architectures with precise control over their compartmentalization.
研究领域:
生物材料学
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纳米生物学
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蛋白质工程
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产品关联
DOI
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-9
·
IF 14.7
Qi Cheng,Ma Xudong,Zeng Qi,Huang Zhangwei,Zhang Shanshan,Deng Xiaokang,Kong Tiantian,Liu Zhou
Hierarchical compartmentalization, a hallmark of both primitive and modern cells, enables the concentration and isolation of biomolecules, and facilitates spatial organization of biochemical reactions. Coacervate-based compartments can sequester and recruit a large variety of molecules, making it an attractive protocell model. In this work, we report the spontaneous formation of core-shell cell-sized coacervate-based compartments driven by spontaneous evaporation of a sessile droplet on a thin-oil-coated substrate. Our analysis reveals that such far-from-equilibrium architectures arise from multiple, coupled segregative and associative liquid-liquid phase separation, and are stabilized by stagnation points within the evaporating droplet. The formation of stagnation points results from convective capillary flows induced by the maximum evaporation rate at the liquid-liquid-air contact line. This work provides valuable insights into the spontaneous formation and maintenance of hierarchical compartments under non-equilibrium conditions, offering a glimpse into the real-life scenario.
研究领域:
化学
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生物物理学
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细胞生物学
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物理化学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 24
·14631-14639
·
IF 3.108
Na Li,Sijing Yi,Zhaohong Qian,Jiao Wang,Nana Lei,Xiao Chen
The unique and interesting aggregation behaviors of a novel surfactant-encapsulated complex (SEC), composed of a Keggin-type polyoxometalate (POM), phosphotungstic acid and a redox-responsive ferrocene-containing cationic surfactant, dimethyldioctadecylammonium bromide (BFDMA), have been investigated in N,N-dimethylformamide (DMF)/butanol mixed solvent. By carefully tuning the composition of the solvent, hollow spherical aggregates with multilayered shells were initially observed with a diameter of 55 ± 9 nm using transmission or scanning electron microscopes. Owing to the solvophobic effect and π–π stacking interaction between alkyl chains or ferrocene groups, these spherical aggregates were found to gradually fuse together into aggregates with flower-like and finally compartment-like morphologies with an average size of 120–150 nm. Based on X-ray diffraction measurements and the molecular configuration data of SEC, the multilayers in aggregates were composed of bilayers of SEC with the POM inside and three rearranged BFDMA molecules outside. The morphology of such aggregates was partially reversible under electrochemical modulation. Applying an anodic oxidation potential would induce the disassembling of compartment-like aggregates into irregular spheres with a size of about 200 nm, which could be turned back after applying a cathodic reduction potential. The obtained results here should shed light on the design of novel nanostructures from such inorganic/organic hybrid clusters.
研究领域:
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Fiber Materials
·
2021
·Vol. 3
·期 6
·383-393
·
IF 0
Lei Fan,Liang Minhua,Liu Yang,Huang Hanhao,Li Haofei,Dong Hua
Organ-on-a-chip (OOC) is now becoming a potential alternative to the classical preclinical animal models, which reconstitutes in vitro the basic function of specific human tissues/organs and dynamically simulates physiological or pathological activities in tissue and organ level. Despite of the much progress achieved so far, there is still an urgent need to explore new biomaterials to construct a reliable and efficient tissue–tissue interface and a general fabrication strategy to expand from single-organ OOC to multi-organ OOC in an easy manner. In this paper, we propose a novel strategy to prepare double-compartment organ-on-a-chip (DC-OOC) using electrospun poly( l -lactic acid)/collagen I (PLLA/Col I) nanofiber membrane as tissue–tissue interface. The unique features of PLLA/Col I nanofiber membrane like excellent biocompatibility, strong affinity to multiple cells, adjustable orientation, controllable thickness and porosity endow the tissue–tissue interface with excellent semi-permeability, appropriate mechanical support, inducible cell orientation, good cell adhesion and proliferation. The integration of 3D printing technology during the fabrication process enables precise size control of the tissue–tissue interface and stable bonding with microfluidic channels. More importantly, our fabrication strategy and OOC configuration makes it easy to extend from DC-OOC to multi-compartment organ-on-a-chip (MC-OOC). To show its possible application, in vitro jaundice disease model is established by constructing blood vessel/skin/liver/lung organ-on-a-chip via MC-OOC. The downward trends of the cell viability after perfusion of bilirubin, the variation in cell sensitivity to bilirubin for different type of cells and recovery of cell viability after blue light therapy prove the feasibility of this jaundice disease model. We believe this general strategy of constructing tissue–tissue interface and multi-organ OOC can be used for many other in vitro physiological and pathological models. Graphic Abstract a Schematic illustration on fabrication process of DC-OOC; b construction of blood vessel/skin/liver/lung organ-on-a-chip as in vitro jaundice disease model
研究领域:
生物医学工程
·
组织工程
·
药物测试
·
疾病建模
✓ 已验证
产品关联
DOI
Research
·
2023
·
IF 11
Chaoyu Yang,Yunru Yu,Yuanjin Zhao,Luoran Shang
Nonspherical particles have attracted increasing interest because of their shape anisotropy. However, the current methods to prepare anisotropic particles suffer from complex generation processes and limited shape diversity. Here, we develop a piezoelectric microfluidic system to generate complex flow configurations and fabricate jellyfish-like microparticles. In this delicate system, the piezoelectric vibration could evolve a jellyfish-like flow configuration in the microchannel and the in situ photopolymerization could instantly capture the flow architecture. The sizes and morphologies of the particles are precisely controlled by tuning the piezoelectric and microfluidic parameters. Furthermore, multi-compartmental microparticles with a dual-layer structure are achieved by modifying the injecting channel geometry. Moreover, such unique a shape endows the particles with flexible motion ability especially when stimuli-responsive materials are incorporated. On the basis of that, we demonstrate the capability of the jellyfish-like microparticles in highly efficient adsorption of organic pollutants under external control. Thus, it is believed that such jellyfish-like microparticles are highly versatile in potential applications and the piezoelectric-integrated microfluidic strategy could open an avenue for the creation of such anisotropic particles.
研究领域:
材料科学
·
微流控技术
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环境科学
·
化学工程
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）