Food Analytical Methods
·
2022
·Vol. 15
·期 5
·1356-1367
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IF 3.498
Gu Yu-Xin,Yan Tian-Ci,Yue Zi-Xuan,Li Min-Hui,Zheng Hui,Wang Shu-Ling,Cao Jun
A novel dispersive micro-solid-phase extraction (DMSPE) method is established for the determination of seven acaricides (clofentezine, benzomate, fenpyroximate, diafenthiuron, pyridaben, doramectin, and ivermectin) in fruit juice and functional food by ultra-high-performance liquid chromatography. In this method, these analytes were extracted from apple juice and curcuma wenyujin using cucurbituril used as the adsorbent, water as the extraction solvent, and methanol as the elution solvent. The primary parameters affecting the extraction efficiency were optimized by experiments for concentration of adsorbent, oscillation time, desorption solvent, and pH value and by response surface methodology. Under the optimal conditions, all target analytes had a good linear relationship, with correlation coefficients ranging from 0.9900 to 0.9940, the limits of detection were 0.42–5.16 ng/mL, the limits of quantification were 1.39–17.18 ng/mL, and the spiked recoveries of seven target compounds in real samples ranged from 70.04 to 94.95%. The intra-day precision RSD variations ranged from 0.13 to 0.70%, and the inter-day precision RSDs were between 0.38 and 1.4%, which had good reproducibility and repeatability. In general, DMSPE is a convenient, efficient, and environmentally friendly method for sample preparation, which has the potential for microextraction.
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高影响因子
BIODEGRADATION
·
2020
·Vol. 31
·期 4
·275-288
·
IF 2.805
Wang Yuanshan,Gong Meihua,Wang Xianlin,Peng Xiaolun,Wang Yuwei,Guan Jiahui,Cheng Dongyuan,Weng Chunyue,Zheng Yuguo
Ivermectin (IVM) is a widely used antiparasitic agent and acaricide. Despite its high efficiency against nematodes and arthropods, IVM may pose a threat to the environment due to its ecotoxcity. In this study, degradation of IVM by a newly isolated bacterium Aeromonas taiwanensis ZJB-18,044 was investigated. Strain ZJB-18,044 can completely degrade 50 mg/L IVM in 5 d with a biodegradation ability of 0.42 mg/L/h. Meanwhile, it exhibited high tolerance (50 mg/L) to doramectin, emamectin, rifampicin, and spiramycin. It can also efficiently degrade doramectin, emamectin, and spiramycin. The IVM degradation of strain ZJB-18,044 can be inhibited by erythromycin, azithromycin, spiramycin or rifampicin. However, supplement of carbonyl cyanide m -chlorophenylhydrazone, an uncoupler of oxidative phosphorylation, can partially recover the IVM degradation. Moreover, strain ZJB-18,044 cells can pump out excess IVM to maintain a low intracellular IVM concentration. Therefore, the IVM tolerance of strain ZJB-18,044 may be due to the regulation of the intracellular IVM concentration by the activated macrolide efflux pump(s). With the high IVM degradation efficiency, A. taiwanensis ZJB-18,044 may serve as a bioremediation agent for IVM and other macrolides in the environment.
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PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2020
·Vol. 85
·期 6
·1681-1688
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IF 2.479
Guanqiong Na,Xiaofei Hu,Yaning Sun,Sharon Kwee,Guangxu Xing,Yunrui Xing,Gaiping Zhang
Valnemulin (VAL) and tiamulin (TIA) are pleuromutilin antibiotics used primarily for treating bacterial infections in swine or other food animals. Furthermore, VAL and TIA are also employed as feed additives to promote animal growth. However, the illegal use of VAL and TIA could cause a series of hazards to consumers. Here, VAL was designed to be conjugated with bovine serum protein to prepare immunogen. A highly sensitive monoclonal antibody that recognized both VAL and TIA has been successfully produced. Moreover, an immunochromatographic strip assay for rapidly screening VAL and TIA in porcine liver was established with visual detection limits (cutoff values) of 50 and 25 ng/g, respectively. The IC 50 values calculated from the equation of the standard curve were 6.06 and 3.45 ng/g and the limits of detection were 0.96 and 0.29 ng/g for VAL and TIA. According to the recovery experiment results, the test strip exhibited acceptable accuracy and precision. Generally, the proposed strip provided a practical tool for the detection of VAL and TIA. Practical Application We produced a highly sensitive monoclonal antibody and developed an immunoassay strip for simultaneously monitoring TIA and VAL. Additionally it was preliminarily confirmed that the rapid detection tool was suitable for screening TIA and VAL in porcine liver.
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高影响因子
Environmental Science-Processes & Impacts
·
2025
·
IF 3.9
Jiawen Ji,Siyue Zhu,Xinping Hu,Jianhui Zhu,Sen Pang,Yongqiang Ma,Xuefeng Li
Abamectin is one of the most widely used pesticides globally, known for its effectiveness in protecting crops and animal health. However, the residual risk of abamectin in agricultural products and the environment may be exacerbated by other pollutants, indicating greater potential hazards. One such emerging environmental pollutant is N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD), a common tire antioxidant found in various environments, including agricultural products and human urine. Our study first revealed the co-existence of 6PPD and abamectin in water and soil, and demonstrated that 6PPD significantly enhances the residual persistence of abamectin in environmental media and vegetables. Specifically, 6PPD extended the half-life of abamectin by 79% in pak choi and by 70% in cabbage. Additionally, 6PPD increased the photolysis half-life of abamectin in water by 191% and by 50% on soil surfaces. Furthermore, 6PPD also prolonged the photolysis half-life of four other macrolides in water. This study reveals the mechanism through which 6PPD extends the half-life of abamectin: by scavenging free radicals and inhibiting hydroxylation and oxidation, thus slowing its degradation. And this paper highlights that 6PPD significantly exacerbates the environmental risks and food safety issue associated with abamectin. Moreover, it provides valuable insights for studying the safe use of pesticides in complex environments with multiple contaminants.
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Mengfan Hu,Huan Xu,Yihong Li,Ziru Lian
Pectenotoxin2 (PTX2), a lipophilic marine biotoxin produced by several species of dinoflagellates, poses significant ecological risks by adversely affecting the growth and survival of fish and shellfish populations. In this study, a highly selective and sensitive ratiometric fluorescence nanoprobe was developed by molecular imprinting technology based on a photoinduced electron transfer recognition mechanism. Zinc oxide (ZnO) nanorods were employed as responsive fluorophore unites, and carbon dots (CDs) extracted from coffee as internal reference signals. Under optimum condition, increasing concentrations of PTX2 in the detection system led to a remarkable quenching of blue-emitting ZnO fluorescence signal, whereas the reference emission from green-emitting CDs remained unaffected. The constructed nanoprobe exhibited a dynamic linear response to PTX2 in the range of 44–1320 ng L −1 with a limit of detection of 13 ng L −1 . Spiked seawater samples yielded satisfactory recoveries from 95.45 % to 99.55 % with relative standard deviations below 9.30 %. Furthermore, semi-quantitative analysis was achieved by integrating a smartphone-based color recognition application, followed by naked-eye visualization under UV irradiation. The dual-emission imprinted fluorescence nanoprobe demonstrates excellent specificity, high sensitivity, and practical applicability for detection of PTX2 at trace levels in seawater with low cost and fast response.
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高影响因子
eLife
·
2025
·
IF 0
Yi Li,Long Gong,Jing Wu,Wesley Hung,Mei Zhen,Shangbang Gao
Resistance to anthelmintics, particularly the macrocyclic lactone ivermectin (IVM), presents a substantial global challenge for parasite control. We found that the functional loss of an evolutionarily conserved E3 ubiquitin ligase, UBR-1, leads to IVM resistance in Caenorhabditis elegans. Multiple IVM-inhibiting activities, including viability, body size, pharyngeal pumping, and locomotion, were significantly ameliorated in various ubr-1 mutants. Interestingly, exogenous application of glutamate induces IVM resistance in wild-type animals. The sensitivity of all IVM-affected phenotypes of ubr-1 is restored by eliminating proteins associated with glutamate metabolism or signaling: GOT-1, a transaminase that converts aspartate to glutamate, and EAT-4, a vesicular glutamate transporter. We demonstrated that IVM-targeted GluCls (glutamate-gated chloride channels) are downregulated and that the IVM-mediated inhibition of serotonin-activated pharynx Ca2+ activity is diminished in ubr-1. Additionally, enhancing glutamate uptake in ubr-1 mutants through ceftriaxone completely restored their IVM sensitivity. Therefore, UBR-1 deficiency-mediated aberrant glutamate signaling leads to ivermectin resistance in C. elegans.
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