Foods
·
2025
·Vol. 14
·期 24
·
IF 5.1
Tailong Wei,Xiao Chen,Chong Cai,Yuanzhen Guo,Mengjun Zhou,Qiannan Gao,Qinghua He
Mimotope-based immunoassays offer an eco-friendly alternative to chemically synthesized antigens for the quantitative analysis of small molecules, but their use for practical on-site and high-throughput residue monitoring remains limited. Herein, we report the selection, production, and application of a phage display–derived mimotope targeting an anti-uniconazole monoclonal antibody (UCZ-mAb), with the aim of developing two complementary immunoassays that enable sensitive, eco-friendly detection of UCZ residues in agricultural samples. A 12-mer phage-displayed peptide library was screened to identify UCZ-specific mimotopes, and a selected sequence was genetically fused to SpyTag and expressed inEscherichia colito generate a SpyTagged mimotope. Leveraging the SpyCatcher/SpyTag self-assembly system, the SpyTagged mimotope was directionally conjugated onto SpyCatcher-functionalized time-resolved fluorescence beads (TRFBs) and subsequently used as a signal-labeled competitive antigen in a lateral flow immunoassay (LFIA) designed for rapid on-site screening. In parallel, a wash-free magnetic separation immunoassay (MSIA) suitable for green, high-throughput screening in routine laboratories was established using self-assembled mimotope-TRFB probes. The LFIA and MSIA exhibited half-maximal inhibitory concentrations (IC50) of 3.70–6.72 μg/kg and 16.4–18.3 μg/kg, respectively, in real samples. Spiked-sample recoveries ranged from 91.1 to 107.8% for LFIA and 92.6–115.7% for MSIA, demonstrating acceptable accuracy and precision. These results indicate that the SpyTagged mimotope–based LFIA and MSIA provide complementary, reliable, and sensitive platforms for on-site screening and high-throughput monitoring of UCZ residues in agricultural samples, while avoiding the drawbacks associated with traditional chemical antigen synthesis.
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高影响因子
Separation Science Plus
·
2026
·Vol. 9
·期 4
·e70218
·
IF 2.7
Xiaowen Hou,Shengnan Jiang,XueLian Qin,Yuhao Xiong,Zhengyi Chen
Paclobutrazol (PBZ) is a widely used triazole plant growth regulator whose residues in fruits, traditional Chinese medicinal materials, and biological matrices raise increasing concerns for food safety and pharmacopoeial quality control. Routine monitoring is dominated by liquid chromatography–tandem mass spectrometry (LC-MS/MS), whereas high-performance LC with ultraviolet detection (HPLC-UV) is rarely used because PBZ absorbs in the deep ultraviolet region near 220 nm, where co-extracted matrix components often cause severe interference. Here, a simple salting-out assisted liquid–liquid extraction procedure based on acetonitrile and sodium sulfate was developed to provide simultaneous clean-up and preconcentration prior to HPLC-UV. Under the optimized conditions, PBZ showed good linearity over 0.5–5.0 µg/mL, with a limit of detection of 0.10 µg/mL. The method was successfully applied to representative complex matrices, including Ophiopogon japonicus , passion fruit, citrus, urine, and plasma, giving recoveries of 83.6%–107% with relative standard deviations ≤4.0%. These results demonstrate that an optimized salting–out–assisted liquid–liquid extraction step can substantially improve the feasibility of deep-ultraviolet detection of PBZ using widely available instrumentation, providing a rapid and cost-effective option for routine screening and quality control.
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yan Zhou,Jinlong Wang,Wangni Meng,Lian Xia,Yue Tang,Jianli Zhou,Yuangen Wu,Pengcheng Yan
Acetamiprid (ACE), a neonicotinoid pesticide broadly used in agricultural production, can accumulate in the food chain due to excessive or incorrect use, thereby posing a threat to human health. In this manuscript , a colorimetric aptasensor assisted by a smartphone was established, relying on the S-18 aptamer-mediated amplification of the laccase-mimic activity of spherical Ag 2 O nanoparticles (NPs). The S-18 aptamer strengthens the interaction and facilitates electron transfer between the Ag 2 O NPs and oxidative substrates, which significantly improves the laccase-mimic activity of the Ag 2 O NPs. When acetamiprid is present, the S-18 aptamer preferentially binds to the target molecule, forming a steady aptamer-acetamiprid complex. This binding interaction prevents the aptamer from promoting the catalytic behavior of the Ag 2 O NPs, producing a distinct and quantifiable color variation in the reaction system. Monitoring the concentration-dependent colorimetric response enables the quick and accurate identification of acetamiprid. The colorimetric aptasensor achieved a limit of detection (LOD) of 8.69 μg∙L −1 markedly lower than the maximum residue limits defined by the national standard (GB 2763–2019) for acetamiprid in fruits and vegetables. Furthermore, the aptasensor demonstrates satisfactory recovery rates in actual samples analyses of cabbage, apple, and tomato, confirming its practical potential for on-site food safety oversight.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Rui Cheng,Yiqun Wan,Xuejin Mao,Hao Wan,Fan Liu,Lan Guo,Bingzhen Zhang
This work presents an efficient sorbent for plant growth regulators (PGRs) by regulating the defects of a metal–organic framework MIL-101(Cr). Using the regulated MIL-101(Cr), we developed a simple and effective method for the simultaneous determination of eleven PGRs in fresh fruit juice. The extraction conditions were optimized by an orthogonal array design. Under optimal conditions, the method showed a satisfactory limit of detection (0.1–1.2 ng/g), recovery rates (83.4–110.2 %), and precision (2.9–18.0 % for intra-day and 2.7–10.8 % for inter-day), as well as a greatly suppressed matrix effect. Notably, regulating the defects significantly enhanced the desorption of PGRs on MIL-101(Cr). The sorbent didn’t need to be destroyed to release the adsorbed PGRs and could be reused at least 6 times. Furthermore, the defects of MIL-101(Cr) and interactions between the sorbent and PGRs were studied by TGA, ATR-IR, XPS, NH 3 -TPD and UV–Vis DRS.
研究领域:
分析化学
·
食品科学
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Analytical Methods
·
2024
·
IF 2.7
Zhihui Guo,Mingya Deng,Wenwen Fang,Kang Zheng,Min Liao,Yulong Wang,Qingkui Fang
Prothioconazole and its metabolite are considered a potential threat to human health and environmental safety. Thus, the development of a sensitive and rapid detection method for prothioconazole is crucial to ensure the safety of agricultural products. In this study, a new hapten of prothioconazole was designed and synthesized, and a selective polyclonal antibody with high affinity against prothioconazole was produced, which was obtained from immunized New Zealand white rabbits. Based on the polyclonal antibody, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and indirect competitive chemiluminescence enzyme immunoassay (ic-CLEIA) were developed for detecting prothioconazole pesticides. Under optimized experimental conditions, the limit of quantification (LOQ) values for ic-CLEIA and ic-ELISA were 1.8 and 10.7 ng mL−1, respectively. The results demonstrated that the sensitivity (LOQ) achieved by ic-CLEIA was more than five times higher compared to that obtained with ic-ELISA. In addition, the recoveries obtained by adding standard prothioconazole to wheat grain, soybean, and pond water samples were in the range of 81.9 to 104.7% for ic-ELISA and 89.0 to 118.0% for ic-CLEIA.
研究领域:
农业化学
·
环境科学
·
分析化学
·
毒理学
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高影响因子
DEVELOPMENTAL CELL
·
2024
·
IF 10.7
Peng He,Liping Zhu,Xin Zhou,Xuan Fu,Yu Zhang,Peng Zhao,Bin Jiang,Huiqin Wang,Guanghui Xiao
The agricultural green revolution spectacularly enhanced crop yield through modification of gibberellin (GA) signaling. However, in cotton, the GA signaling cascades remain elusive, limiting our potential to cultivate new cotton varieties and improve yield and quality. Here, we identified that GA prominently stimulated fiber elongation through the degradation of DELLA protein GhSLR1, thereby disabling GhSLR1's physical interaction with two transcription factors, GhZFP8 and GhBLH1. Subsequently, the resultant free GhBLH1 binds to GhKCS12 promoter and activates its expression to enhance VLCFAs biosynthesis. With a similar mechanism, the free GhZFP8 binds to GhSDCP1 promoter and activates its expression. As a result, GhSDCP1 upregulates the expression of GhPIF3 gene associated with plant cell elongation. Ultimately, the two parallel signaling cascades synergistically promote cotton fiber elongation. Our findings outline the mechanistic framework that translates the GA signal into fiber cell elongation, thereby offering a roadmap to improve cotton fiber quality and yield.
研究领域:
植物学
·
遗传学
·
植物生理学
·
分子生物学
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高影响因子
Frontiers in Pharmacology
·
2021
·
IF 5.811
Yu Kunzi,Liu Wei,Zhang Nanping,Cheng Xianlong,Zhou Shiyu,Zuo Tiantian,Kang Shuai,Wei Feng,Ma Shuangcheng
Maidong, the root tuber of Ophiopogon japonicus (Thunb.) Ker Gawl., is a commonly used herbal medicine in China. There are three quality grades of Maidong according to traditional opinion and modern research studies: superior quality (Zhe-Maidong), medium quality (Chuan-Maidong), and poorest quality (Chuan-Maidong with paclobutrazol, which is a kind of plant growth regulator). However, no efficient way to distinguish the three quality grades of Maidong exists; thus, the herbal markets and botanical pharmacies are flooded with Chuan-Maidong with paclobutrazol. To ensure the safety and quality of Maidong, a comparative microscopic study was performed on three quality grades of Maidong. The result was to establish a microscopic quantification method based on the area ratio between xylem and pith to distinguish the three quality grades of Maidong. Subsequently, Maidong from regional markets was evaluated by this method. In this study, we developed a novel quantification method to identify the three quality grades of Maidong, which could in turn make efforts on the quality improvement of Maidong. Our study is the first to demonstrate that microscopic technology could be used to distinguish different quality grades of a specific herbal medicine.
研究领域:
中药学
·
植物显微学
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FOOD CHEMISTRY
·
2019
·
IF 5.399
Jing Zhao,Zhaorui Meng,Zexin Zhao,Longshan Zhao
A green and highly efficient ultrasound-assisted deep eutectic solvent extraction combined with functionalized magnetic multi-walled carbon nanotubes solid-phase extraction method for determination of seven pesticide residues in food products was developed. Various types of deep eutectic solvents (DESs) were screened for high extraction efficiencies and DES composing of proline and propylene glycol at 1:3 M ratio was selected as it exhibited highest yields. Extraction conditions were statistically optimized through response surface methodology using a Box-Behnken design. The established method was linear, precise, and accurate over the range of 0.1–50 μg mL −1 . Limit of detection and limit of quantification were in the range of 0.02–0.05 μg mL −1 and 0.05–0.10 μg mL −1 , respectively. The mean recoveries for pesticides ranged from 76.09 to 97.96% with relative standard deviation of 0.13–10.05%. The proposed method was successfully applied to analysis of the pesticides in real samples, which is a potentially promising technique for food matrix.
研究领域:
分析化学
·
食品安全
·
环境科学
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Yulong Wang,Junli Xu,Yulou Qiu,Pan Li,Beibei Liu,Lifei Yang,Bogdan Barnych,Bruce D. Hammock,Cunzheng Zhang
A monoclonal antibody (mAb) was raised against tebuconazole (TEB) using a hapten where the p-chloro substituent of the TEB molecule was replaced with a long-chain carboxylic acid. The resulting mAb showed high sensitivity and specificity against TEB characterized by ELISA with a half-maximal inhibitory concentration (IC50) of 0.19 ng mL–1 and with cross-reactivity (CR) values below 0.01% to several analogues of triazole fungicides. On the basis of the mAb produced, a quantum dot beads-based fluorescence immunochromatographic test strip assay (QBs–FITSA) was developed for rapid and sensitive detection of TEB in agricultural product samples. The QBs–FITSA exhibited a linear detection range from 0.02 to 1.25 ng mL–1 with a limit of detection (LOD) of 0.02 ng mL–1. Furthermore, using produced mAb, multiple high-throughput rapid immunoassay formats could be achieved as a convenient monitoring tool for evaluation of human and environmental exposure to TEB.
研究领域:
分析化学
·
食品科学
·
环境科学
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Analytical Methods
·
2021
·Vol. 13
·期 29
·3282-3291
·
IF 2.896
Jiaxin Zhang,Tingting Feng,Jiayu Zhang,Ning Liang,Longshan Zhao
A simple, quick, effective turn-on fluorescence assay for the determination of fipronil (FIP) was built based on the yellow fluorescence of FAM-aptamer and excellent quenching capability of the oxidized single-walled carbon nanohorns (The oxidized SWCNHs). Oxidized SWCNHs with the great advantage of good dispersibility in solution were generated by link to carboxyl group and were added to a specific FAM-aptamer at an optimal concentration to form an “on–off” oxidized SWCNH/FAM-aptamer fluorescent sensor. The structures of the oxidized SWCNHs were verified, and the comprehensive properties were evaluated by characterization techniques. This paper has exploited oxidized SWCNHs as a quenching agent to detect fipronil for the first time. Under the optimized conditions, the limit of detection (LOD) for fipronil was 3 nM, and the recovery of fipronil varied from 88.6% to 112.7% in different real samples with relative standard deviations (RSDs) not more than 5%. The developed method could be successfully applied for the determination of fipronil in tap water, honey and corn samples.
研究领域:
分析化学
·
环境科学
·
食品安全
·
纳米技术
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高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Qingqing Zhang,Xuejin Mao,Changrong Yuan,Jiexue Zhao,Hui Hu,Aiping Yan,Yuanxing Wang,Weiming Xiao
A facile, efficient and reliable method was designed and established to analyze the plant growth regulators (PGRs) in citrus fruit, based on a simplified dispersive solid-phase extraction (d-SPE) using a shaped zirconium-based metal–organic framework (UiO-66-PDMS bead) as the sorbent. In this method, UiO-66-PDMS beads directly extracted the targets from the homogenized and could be easily separated with a tweezer. It avoided the centrifugation or filtration operation required in normal d-SPE, greatly simplifying the d-SPE process. Moreover, the matrix substances were efficiently removed by this d-SPE process. The method showed good linearities (R 2 ≥ 0.9995) and limits of detection (0.09–0.17 ng/g). The recoveries were in the range of 80.7–97.5 %. The intra-day and inter-day precisions were 1.5–6.3 % and 4.6–11.7 %, respectively. Additionally, the adsorption interactions between UiO-66-PDMS bead and PGRs were studied by ATR-FTIR and UV–vis DRS. Furthermore, the method was employed to screen the PGRs in ten different citrus fruits.
研究领域:
分析化学
·
食品科学
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高影响因子
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