Frontiers in Microbiology
·
2024
·
IF 4
Xu Chong,Pan Danyang,Zhang Dexiang,Lin Lin,Chen Yiti,Liang Shuangcheng,He Jingyu
Introduction: Microorganisms as a component of scalp ecosystem play a vital role in human scalp health. Soapberry pericarp is employed in improving scalp health, and its content of active ingredients could be enhanced resulted in fermentation. This study aims to investigate the effect of the fermentation filtrate from soapberry pericarp on the diversity of scalp microorganisms.Methods: The components in fermentation filtrate from soapberry pericarp were analyzed by HPLC-Q-Orbitrap HRMS, and 16S and ITS sequences of 198 samples from three different used stages (Day 0, Day 7, Day 28) were sequenced using the Illumina Novaseq platform. Microbial diversity was assessed using alpha diversity (Chao1 and Shannon indexes) and beta diversity (weighted unifrac and unweighted unifrac). Principal coordinate analysis (PCoA) and linear discriminant analysis (LDA) effect size analysis (LEfSe) were used to visualize microbial variation among different stages.Results: There were 22 components were identified in the fermentation filtrate from soapberry pericarp by HPLC-Q-Orbitrap HRMS. The alpha-diversity and beta-diversity analyses showed that scalp microbial diversity and composition were influenced by the fermentation filtrate of soapberry pericarp. Based on functional analysis, this study found an enrichment of healthy scalp-related bacterial pathways, such as amino acid, nucleoside, and nucleotide biosynthesis, while a decrease in fungal pathogenesis pathways, specifically saprotroph and symbiotroph pathways, was observed.Discussion: The study described about the complex community dynamics of human scalp microorganisms during the stages of using the fermentation filtrate from soapberry pericarp. This result will help rationally utilize the fermentation filtrate from soapberry pericarp to keep or improve human scalp health.
研究领域:
微生物学
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药学
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生物技术
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皮肤科
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DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2022
·Vol. 105
·期 6
·4512-4520
·
IF 4.186
Shuaichen Si,Xiaoxuan Guo,Simin Gu,Lin Huang,Chong-Geng Ma,Bingfu Lei,Uygun V. Valiev,Jing Wang
Laser sources have been considered to be better light sources compared to traditional lamps or light-emitting diodes used in projectors, which enables the projector to create images with higher color saturation, brightness, and energy efficiency. However, the speckle noise caused by the coherent nature of the laser is a major technology obstacle for laser projection, which severely affects the image quality. To suppress laser speckle, a TiO 2 -sapphire composite (TSC) with rough and high reflectivity surface and high thermal conductivity substrate is designed, which reduces the laser speckle by creating spatial diversity. Moreover, combined with a micro motor, the design and fabrication of the TSC rotating wheel are realized, which further reduces the laser speckle by creating time diversity. The experimental results show that the laser speckle contrast can be reduced from 9.0% to 2.2% when placing the TSC rotating wheel in the light path, which is below the speckle perception limit of the human eye (<4%). This new type of laser speckle suppression device is simple to use, low in cost, high in energy utilization, high in thermal conductivity, easy to mass production, and has great application potential in high-power laser projection.
研究领域:
光学
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光子学
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材料科学
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激光技术
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投影系统
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DOI
高影响因子
PHYTOPATHOLOGY
·
2014
·Vol. 104
·期 10
·
IF 2.746
Amara R. Dunn,Stephen R. Bruening,Niklaus J. Grünwald,Christine D. Smart
Populations of the vegetable pathogen Phytophthora capsici are often highly diverse, with limited gene flow between fields. To investigate the structure of a newly established, experimental population, an uninfested research field was inoculated with two single-zoospore isolates of P. capsici in September 2008. From 2009 through 2012, ≈50 isolates of P. capsici were collected from the field each year and genotyped using five microsatellite loci. The same two isolates were also crossed in the lab. High levels of diversity were detected in the research field, with 26 to 37 unique multilocus genotypes detected each year. Through 2012, genotypic diversity did not decline and no evidence of genetic drift was observed. However, during the 2011 and 2012 growing seasons, four new alleles not present in either parental isolate were observed in the field. Selfing (but not apomixis) was observed at low frequency among in vitro progeny. In addition, evidence for loss of heterozygosity was observed in half of the in vitro progeny. These results suggest that recombination, mutation, and loss of heterozygosity can affect the genetic structure observed in P. capsici populations.
研究领域:
植物病理学
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遗传学
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DOI
高影响因子
Processes
·
2019
·Vol. 7
·期 11
·827
·
IF 1.963
Yong Li,Siyuan Zhao,Jiejie Zhang,Yang He,Jianqiang Zhang,Rong Ge
The aerobic denitrifying phosphate accumulating bacteria (ADPB) use NO3−as an electron acceptor and remove nitrate by denitrification and concomitant uptake of excessive phosphorus in aerobic conditions. Activated sludge was collected from the A2O aerobic biological pool of the sewage treatment plant at Hezuo Town, Chengdu City. The candidate ADPB strains were obtained by cultivation in the enriched denitrification media, followed by repeated isolation and purification on bromothymol blue (BTB) solid plates. The obtained candidates were further screened for ADPB strains by phosphorus uptake experiment, nitrate reduction test, metachromatic granules staining, and poly-β-hydroxybutyrate (PHB) staining. The 16 sedimentation ribosome deoxyribonucleic acid (16 S rDNA) molecular technique was used to determine their taxonomy. Further, the denitrification and dephosphorization capacities of ADPB strains were ascertained through their growth characteristics in nitrogen-phosphorus-rich liquid media. The results revealed a total of 25 ADPB strains screened from the activated sludge of the A2O aerobic biological pool of the sewage treatment plant at Hezuo Town. These strains belonged to two classes, four orders, and five genera. Among them, the strain SW18NP2 was a potentially new species in theAcinetobactergenus, while the strain SW18NP24 was a potential new species in thePseudomonasgenus at the time of their characterization. TheAcinetobacterwas the dominant genus. The obtained ADPB strains demonstrated a rich diversity. The ADPB strains had significant variations in denitrification and dephosphorization capacities. Twenty-three strains exhibited a total phosphorus removal rate of above 50%, and 19 strains exhibited a total nitrogen removal rate of above 50%. The strain SW18NP2 showed the best denitrifying phosphorus removal (DPR) capacity, with a dephosphorization rate of 82.32% and a denitrification rate of 73.73%. The ADPB in the A2O aerobic biological pool of the sewage treatment plant at Hezuo Town demonstrated a rich diversity and a strong DPR capacity.Keywords:aerobic denitrifying phosphate accumulating bacteria (ADPB);diversity;denitrifying phosphorus removal
研究领域:
环境微生物学
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污水处理
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
·
IF 6.2
Haichun Zeng,Beilin Meng,Jiatong Zeng,Li Rao
Sesquiterpenes, a structurally diverse class of natural products, play pivotal roles in the pharmaceutical, fragrance, and flavor industries. In this study, we first identified and functionally characterized a multifunctional sesquiterpene synthase, ChTPS6, from the plant-pathogenic fungus Cochliobolus heterostrophus. When incubated with FPP, ChTPS6 primarily catalyzed the formation of cubebol as the main product, which is widely used as a sustained cooling and refreshing agent in the food industry. Remarkably, ChTPS6 exhibited substrate promiscuity, efficiently utilizing GPP and GGPP as alternative substrates. Moreover, through protein modeling and site-directed mutagenesis, we pinpointed key amino acid residues essential for the catalytic activity of ChTPS6, and successfully re-engineered the enzyme to alter its product specificity. These findings provide novel insights into the catalytic mechanism and functional versatility of ChTPS6 while broadening its potential applications in the biosynthesis of valuable sesquiterpenes.
研究领域:
生物化学
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分子生物学
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真菌学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CATALYSIS
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2021
·
IF 8.271
Yan-Cheng Hu,Ying Li,Ding-Wei Ji,Heng Liu,Hao Zheng,Gong Zhang,Qing-An Chen
C2 prenylated indoles are widespread in a variety of bioactive natural alkaloids. Therefore, the selective installation of prenyl group at C2 position of NH indoles is of great significance. However, the known protocols generally require a multi-step procedure and stoichiometric promoters. Herein we develop a one-step C2 prenylation of NH indole with cheap tert- prenyl alcohol enabled by acid catalysis. Salient features include good regioselectivity, step- and atom-economy, broad substrate scope, and simple catalytic system. The mechanistic investigations demonstrate that both C2 prenylation and C3 prenylation/migration pathways are engaged in the reaction. Notably, this practical strategy can be applied to the late-stage diversification of tryptophan-based peptides and concise synthesis of tryprostatin B.
研究领域:
有机化学
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药物化学
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合成化学
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2022
·
IF 10.753
Bingfeng Chen,Yizhou Pan,Yiling Chen,Zhenyan Zhang,Zhihan Yang,Meng Zheng,Tao Lu,Liying Jiang,Haifeng Qian
Titanium dioxide nanoparticle (n-TiO 2 ) is a widely used nanomaterial, which is inevitably released as a residue into aquatic ecosystems during material production and usage. However, the effects of n-TiO 2 on aquatic microbial communities have not been completely elucidated. This study examined the toxic effects of n-TiO 2 on eukaryotic and prokaryotic microbial communities in freshwater environments. We determined that n-TiO 2 had a greater inhibitory effect on the growth of eukaryotic algae than cyanobacteria in monocultures. A similar phenomenon was observed in a microcosm experiment, revealing that n-TiO 2 slightly reduced the content of chlorophyll-a but evidently increased the phycocyanin content. Moreover, the alpha diversity of the eukaryotic community was not affected, whereas its beta diversity increased with exposure to n-TiO 2 . Although n-TiO 2 altered the composition of freshwater microbial communities, it did not change the functions of the prokaryotic community, which might be attributed to the functional redundancy of microbiota. Co-occurrence network analysis indicated that n-TiO 2 destabilized the freshwater community, especially the eukaryotic community, and potentially disturbed the aquatic ecosystem. Our study revealed that the ecological risk of n-TiO 2 on aquatic microbial communities is complex; hence, rational utilization of n-TiO 2 should be emphasized.
研究领域:
环境科学
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微生物学
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生态学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL ENGINEERING
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2023
·Vol. 150
·期 2
·04023101
·
IF 2.2
Lei Feng,Chenxi Liu,Jianan Song
In order to investigate the effect of compound antibiotics on anaerobic digestion of chicken manure, the inhibitory effect of three different combinations of oxytetracycline (OTC), sulfamethoxazole (SMZ), and enrofloxacin (ENR) on anaerobic digestion of chicken manure was studied by the sequencing batch method. Under the condition of adding three different combinations of antibiotics, the experiment was run for 50 days. The concentrations of soluble chemical oxygen demand (SCOD), total ammonia nitrogen (TAN), VFAs, and methane production in the reactor were measured, and the microbial diversity affecting the stability of the system was analyzed. The results showed that OTC and SMZ inhibited methane production, while SMZ and ENR promoted methane production, and the cumulative methane production was R4>R3>R1>R2. After the 25th day, the SCOD concentration in the R2 and R4 groups decreased, which was due to the existence of SMZ and ENR promoting the degradation of SCOD. TAN concentrations in the four groups were kept between 1,190 and 2,100 mg/L. The results of 16S rRNA sequencing showed that the mixed antibiotics had different effects on the bacterial community, which was mainly composed of Firmicutes, Bacteroidota, unidentified bacteria, Cloacimonadota, Proteobacteria, and Spirochaetota. Bacteroidota was superior in the whole reaction process under the influence of OTC and SMZ because it stimulated the microbial flora and made its activity higher. The study provides relevant data for the proper management of livestock manure.
研究领域:
环境科学
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微生物学
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DOI
高影响因子
CHINESE JOURNAL OF CHEMISTRY
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2015
·Vol. 33
·期 9
·1084-1088
·
IF 1.578
Yafang Tan,Xiaohui Sun,Fuyue Dong,Haiyan Tian,Renwang Jiang
To enhance the structural diversity of tanshinones and provide more derivatives for the biological studies, a one-pot combinatorial modification strategy was performed on total tanshinones. Six new quinoxlinetanshinones 1 – 6 were subsequently isolated from the combinatorial modified semi-synthetic mixture. The structures were elucidated by spectroscopic analysis in combination with single-crystal X-ray diffraction. These quinoxlinetanshinones demonstrated strong DNA binding properties. Rapid synthesis of new quinoxlinetanshinones with significant biological activity highlights the great potential of one-pot combinatorial modification for the diversification of natural products.
研究领域:
药理学
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有机化学
·
天然产物研究
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Tao Li,Yuhua Li,Xike Zhang,Jiawei Yuan,Junkang Guo,Pan Wang,Gehong Wei,Chun Chen
The widespread application of metal-based nanoparticle (MNPs)/reduced graphene oxide (rGO) composites inevitably leads to their release into soils. However, we lack a detailed understanding of the bacterial community response to MNPs-rGO exposure in farmland soils. Here, we conducted a soil microcosm experiment to analyze the potential impact of MNPs-rGO on bacterial communities in two field soils via high-throughput sequencing. The change in alpha diversity of bacterial communities was more susceptible to Ag-rGO and ZnO-rGO treatments than CuO-rGO. In both soils, MNPs-rGO significantly changed the bacterial community structure even at a low dose (1 mg kg −1 ). The bacterial community structure was most strongly affected by Ag-rGO at 30 days, but the greatest changes occurred in ZnO-rGO at 60 days. The differences in soil properties could shape bacterial communities to MNPs-rGO exposure. Distance-based redundancy analysis and functional annotation of prokaryotic taxa showed that some bacterial species associated with nitrogen cycling were greatly influenced by Ag-rGO and ZnO-rGO exposure. In sum, Ag-rGO and ZnO-rGO may potentially affect bacterial communities and nitrogen turnover under long-term realistic field exposure. These findings present a perspective on the response of bacterial communities to MNPs-rGO and provide a fundamental basis for estimating the ecological behavior of MNPs-rGO.
研究领域:
环境科学
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微生物学
·
土壤学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
·
2022
·Vol. 76
·期 1
·51-62
·
IF 4.286
Huaxiang Li,Yanhe Zhang,Xinhai Yuan,Siqi Liu,Luyao Fan,Xiangfeng Zheng,Shuo Wang,Lei Yuan,Xinan Jiao
This study aimed to reveal microbial populations in Chinese raw milk using both 16S rRNA sequencing and high-throughput sequence (HTS) methods. Psychrotrophic isolates were clustered into 23 genera and 57 species, and the abundant genera in raw milk were Pseudomonas , Acinetobacter and Lactococcus . Genetic diversity and biofilm-forming capacity of Pseudomonas isolates were analysed using random amplified polymorphic DNA (RAPD) and crystal violet staining assay, respectively. Heterogeneous biofilm-forming ability of Pseudomonas isolates with the same RAPD patterns was observed. HTS analysis revealed a 33-fold higher diversity at the genus level, and a different bacterial population was predominated by Flavobacterium , Pseudomonas and Acinetobacter . The results provide insight into the microbial contamination of raw milk.
研究领域:
微生物学
·
食品科学
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Guorong Fan,Xiaodan Ning,Shangxing Chen,Liang Zhong,Chunce Guo,Yuling Yang,Juan Liu,Ming Tang,Guoxiang Liao,Xuefang Wang,Zongde Wang,Jiawei Wang
Litsea cubeba (Lour.) Pers. is an important industrial tree in southern China and Southeast Asia. We surveyed and sampled wild L. cubeba in 32 provenances spread across nine provinces of China. We used nested variance analysis, multiple comparisons analysis, hierarchical clustering analysis, Pearson’s correlation analysis, path analysis with multiple stepwise regression, detrended correspondence analysis, redundancy analysis, and variance partitioning analysis to evaluate the phenotypic diversity of fresh fruit yield (FFY) and essential oil (EO) characteristics of L. cubeba , and to explore the relationship between the main habitat factors and phenotypic diversity. We detected wide phenotypic diversity in FFY, EO content (EOC), citral yield (CitrY), and the proportions of the seven main components of EOs (citral, D -limonene, 3,7-dimethyl-3,6-octadienal, 4-methyl-3-pentenal, linalool , citronellal , and 1-(cyclopanecarbonyl)piperidin-4-one). The variation was mainly derived from among provenances ( P ≤ 0.01) rather than within provenances, and the variation in FFY and CitrY was greater than that of EOC and the seven dominant components among provenances. We also detected several region-specific components of EOs in fresh fruit, including α-terpineol, geraniol , eucalyptol , and 7-methyl-3-methyleneoct-6-enal. Most of the provenances with higher FFY were distributed in southwestern China (lower longitude and latitude, and higher elevation), while most of those with higher EOC were distributed in southeastern China (higher longitude and latitude, and lower elevation). The three provenances with excellent FFY were YN-XC, GZ-ZY, and SC-KJ; the two with higher EOC were SC-KJ and HN-DX; and CitrP was higher in GZ-ZF than in other provenances. Longitude, total potassium content (TotalKC), and bulk density (BD) were the most important factors that influenced FFY diversity, while latitude, elevation, annual average relative humidity (AARH), and organic carbon content (OCC) most strongly influenced EOC diversity, and annual average temperature and annual average maximum temperature (AAMaxT) influenced CitrP diversity. The six main habitat factors together explaining the largest proportions of phenotypic diversity were longitude, AARH, AAMaxT, elevation, latitude, and soil total phosphorus content (TotalPC). Higher soil TotalKC, BD, and TotalPC were suitable conditions for cultivation of L. cubeba to achieve higher FFY, while lower AARH and OCC were favorable for accumulation of EOs, and only lower temperature was more likely conducive to citral synthesis. The wide diversity of FFY and EO characteristics should be taken into account when selecting germplasms for protection and utilization, and the identification of the most important environmental factors affecting phenotypic diversity provides a theoretical basis for the optimization of L. cubeba cultivation.
研究领域:
植物学
·
生态学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）