Frontiers in Microbiology
·
2024
·
IF 4
Yu Biao,Lu Zheng,Zhong Saiyi,Cheong Kit-Leong
Introduction: Research on the mechanism of marine polysaccharide utilization by Bacteroides thetaiotaomicron has drawn substantial attention in recent years. Derived from marine algae, the marine algae polysaccharides could serve as prebiotics to facilitate intestinal microecological balance and alleviate colonic diseases. Bacteroides thetaiotaomicron, considered the most efficient degrader of polysaccharides, relates to its capacity to degrade an extensive spectrum of complex polysaccharides. Polysaccharide utilization loci (PULs), a specialized organization of a collection of genes-encoded enzymes engaged in the breakdown and utilization of polysaccharides, make it possible for Bacteroides thetaiotaomicron to metabolize various polysaccharides. However, there is still a paucity of comprehensive studies on the procedure of polysaccharide degradation by Bacteroides thetaiotaomicron.Methods: In the current study, the degradation of four kinds of marine algae polysaccharides, including sodium alginate, fucoidan, laminarin, and Pyropia haitanensis polysaccharides, and the underlying mechanism by Bacteroides thetaiotaomicron G4 were investigated. Pure culture of Bacteroides thetaiotaomicron G4 in a substrate supplemented with these polysaccharides were performed. The change of OD600, total carbohydrate contents, and molecular weight during this fermentation were determined. Genomic sequencing and bioinformatic analysis were further performed to elucidate the mechanisms involved. Specifically, Gene Ontology (GO) annotation, Clusters of Orthologous Groups (COG) annotation, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment were utilized to identify potential target genes and pathways.Results: Underlying target genes and pathways were recognized by employing bioinformatic analysis. Several PULs were found that are anticipated to participate in the breakdown of these four polysaccharides. These findings may help to understand the interactions between these marine seaweed polysaccharides and gut microorganisms.Discussion: The elucidation of polysaccharide degradation mechanisms by Bacteroides thetaiotaomicron provides valuable insights into the utilization of marine polysaccharides as prebiotics and their potential impact on gut health. Further studies are warranted to explore the specific roles of individual PULs and their contributions to polysaccharide metabolism in the gut microbiota.
研究领域:
微生物学
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分子生物学
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基因组学
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生物信息学
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营养科学
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胃肠病学
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产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2021
·
IF 4.451
Xizhu Wang,Liyan Yuan,Zhijie Bao,Baoshang Fu,Pengfei Jiang,Tiecheng Ma,Songyi Lin
To screen the in vitro uric acid-lowering activity of the neutral polysaccharides (NCSP) and acid polysaccharides (ACSP) isolated from the corn silk were evaluated. Furthermore, preliminary structure-activity relationships for them were investigated. The molecular weight (Mw) of NCSP and ACSP were 74.1 and 109.4 kDa, respectively. NCSP consisted of mannose (Man), glucuronic acid (GlcA), glucose (Glc), galactose (Gal), and arabinose (Ara), in addition to these monosaccharides, the ACSP contains galactose acid (GalA). NMR spectroscopy showed that the NCSP main chain consisted of 1,4,6)- α -D-Glc p , 1,4)- α -D-Glc p , 1,3)- β -D-Gal p , and 1,3,5)- β -D-Man p . NCSP exhibited higher activity in anti-hyperuricemia (HUA). The results indicated that serum uric acid (UA) and liver xanthine oxidase (XO) activities levels decreased by 45.71% and 22.4% in the NCSP groups, respectively. In addition, NCSP could significantly improve the kidney damage and promote uric acid excretion. These findings provide evidence in support of NCSP as a potential dietary supplement for improving HUA.
研究领域:
药理学
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生物化学
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营养学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
·
IF 5.162
Yong-Gang Xia,Yong-Xin Huang,Jun Liang,Hai-Xue Kuang
Two pectin-polysaccharides, ASP-B 2 (M w , 5.32 kDa) and B 3 (M w , 30.51 kDa), were obtained from Acanthopanax senticosus leaves. An HILIC-UPLC-ESI − -HCD-MS/MS technique was introduced for structural elucidations of skeletal structure of pectin-polysaccharides in addition to empolying SEC-MALLS, GC–MS, UPLC-ESI + -MS, methylation analysis, FT-IR and NMR methods . Skeletal structure of ASP-B 2 was characterized by combining HG smooth region and RG hairy region with a 2:1 chain length ratio, wheres ASP-B 3 had a distinguishing 3:1 chain length ratio (HG:RG). HG were defined as repeated → 4 GalA 1 →, and RG were featured by repeated → 4 GalA 1 → 2 Rha p 1 → with branched points at C-3, C-4 and C-3,4 of rhamnosyl. Side chains mainly consisted of galactans , arabinans and arabinogalactans. Both showed a random coil conformation and no triple-helix conformation. ASP-B 2 and B 3 possess stronger DPPH and hydroxyl radical scavenging activities, with a relatively low reducing power. Thus, two polysaccharides can be further explored as novel natural antioxidant.
研究领域:
植物化学
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天然产物化学
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食品科学
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DOI
PubMed
高影响因子
Chinese Journal of Natural Medicines
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2017
·
IF 1.667
Xiao-Xiao LU,Yi-Fan JIANG,Hong LI,Ying-Ye OU,Zhi-De ZHANG,Hong-Ye DI,Dao-Feng CHEN,Yun-Yi ZHANG
Lipopolysaccharides (LPS) contamination in herbal crude polysaccharides is inevitable. The present study was performed to explore the effect of polymyxin B on abolishing the influence of LPS contamination in mononuclear cells. LPS was pretreated with polymyxin B sulfate (PB) at different concentrations for 1, 5 or 24 h, and then used to stimulate RAW264.7 and mouse peritoneal macrophages (MPMs). The nitric oxide (NO) and tumor necrosis factor-α (TNF-α) in cell culture supernatant, as the indications of cell response, were assayed. Bupleurum chinensis polysaccharides (BCPs) with trace amount contamination of LPS was treated with PB. 30 μg·mL −1 of PB, treating LPS (10 and 1 000 ng·mL −1 in stimulating RAW264.7 and MPMs respectively) at 37 °C for 24 h, successfully abolished the stimulating effect of LPS on the cells. When the cells were stimulated with LPS, BCPs further promoted NO production. However, pretreated with PB, BCPs showed a suppression of NO production in MPMs and no change in RAW264.7. In the in vitro experiments, LPS contamination in polysaccharide might bring a great interference in assessing the activity of drug. Pretreatment with PB (30 μg·mL −1 ) at 37 °C for 24 h was sufficient to abolish the effects of LPS contamination (10 and 1 000 ng·mL −1 ).
研究领域:
免疫学
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药理学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2018
·
IF 6.042
Huiying Ma,Qing Jiang,Diya Dai,Hongli Li,Wentao Bi,David Da Yong Chen
Polysaccharide characterization posts the most difficult challenge to available analytical technologies compared to other types of biomolecules. Plant polysaccharides are reported to have numerous medicinal values, but their effect can be different based on the types of plants, and even regions of productions and conditions of cultivation. However, the molecular basis of the differences of these polysaccharides is largely unknown. In this study, direct analysis in real time mass spectrometry (DART-MS) was used to generate polysaccharide fingerprints. Large saccharides can break down into characteristic small fragments in the DART source via pyrolysis, and the products are then detected by high resolution MS. Temperature was shown to be a crucial parameter for the decomposition of large polysaccharide. The general behavior of carbohydrates in DART-MS was also studied through the investigation of a number of mono- and oligosaccharide standards. The chemical formula and putative ionic forms of the fragments were proposed based on accurate mass with less than 10 ppm mass errors. Multivariate data analysis shows the clear differentiation of different plant species. Intensities of marker ions compared among samples also showed obvious differences. The combination of DART-MS analysis and mechanochemical extraction method used in this work demonstrates a simple, fast, and high throughput analytical protocol for the efficient evaluation of molecular features in plant polysaccharides.
研究领域:
分析化学
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植物学
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药理学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Yanyun Zhu,Yunyun Zheng,Bingge Liu,Kouadio Jean Eric-Parfait Kouame,Ebenezer O. Falade,Jianchu Chen,Xingqian Ye
Monosaccharide composition and glycosidic linkages analysis are essential for the structural characterization and biological activity research of polysaccharides. To simplify the analysis steps and improve detection efficiency, this study developed monosaccharide compositions and glycosidic linkages detection methods based on UPLC-QqQ-MS/MS, and established a plant polysaccharide glycosidic linkages library. Furthermore, the detailed analysis process of monosaccharide compositions and glycosidic linkages was presented through a plant polysaccharide (Chinese bayberry wine polysaccharide, CPW) example. The results showed that the monosaccharide analysis method could identify and quantify 20 monosaccharides within 13 min by 6 chromatograms. By this method, 7 monosaccharides were detected in CPW, which was consistent with previous results obtained using ion chromatography. Meanwhile, the UPLC-QqQ-MS/MS based method could analyze 112 glycosidic linkages of 9 plant monosaccharides within 30 min, and the results were distributed on 11 chromatograms. According to the approach, 44 different glycosidic linkages were identified in CPW. Based on Congo red assay and molecular weight as well as our findings of monosaccharide composition and glycosidic linkages, we proposed that CPW may consist of two distinct sugar chains: One composed of 1–2,4-Rha, 1–3,4-GalA, and 1-F-Ara, and the other composed of 1–3,4-Gal, 1–4-Gal, 1–3,4,6-Gal, and 1-F-Ara.
研究领域:
分析化学
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植物学
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生物化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Qianni Chen,Jiaqi Fan,Lianzhu Lin,Mouming Zhao
The combination of polysaccharides is an effective way to develop prebiotics with stable performance during processing and digestion for human wellness. However, there is little information on optimal screening and complementary regulation of compound polysaccharides. This study aimed to optimally select a combination of Lycium barbarum L. polysaccharide (LBP) and Laminaria japonica polysaccharide (LJP) as a highly efficient prebiotic to regulate the gut probiotics and their metabolites. Two LBPs characterized as rhamnogalacturonan I enriched pectins and two LJPs characterized as fucoidans were obtained by enzyme-assisted acid extraction at moderate and dramatic temperatures and combined in pairs to obtain 4 groups containing 4 proportional combinations. All combinations showed better prebiotic effects than individual LJP. The combination of LBP and LJP extracted at 50 °C at a ratio of 4:1 exhibited the strongest prebiotic effect. The optimal compound polysaccharide achieved superior effect and complementary function via LBP-targeted proliferation of Bifidobacterium , Lactobacillus , and Bacteroides and production of SCFAs and non-SCFA health-associated metabolites, LJP-targeted accumulation of butyrate-producing bacteria and corresponding metabolites, as well as synergistic effect of LJP and LBP at exact proportion. Our study provided theoretical and methodological guidance for optimal screening of compound polysaccharides as new prebiotics.
研究领域:
食品科学
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微生物学
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营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2014
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IF 4.28
Fei Zhang,Tu Luan,Ding Kang,Qiangwei Jin,Hongbin Zhang,Madhav P. Yadav
The effect of corn fiber gum (CFG) on the aqueous solutions of a series of widely-used commercial polysaccharides has been studied by rheological techniques using a shear stress synergism index I S to evaluate its viscosifying action. While CFG solution exhibited Newtonian fluid behavior with a very low viscosity even at a high concentration, the aqueous mixtures of CFG and some non-gelling polysaccharides (hyaluronan, guar gum , carboxymethylcellulose, hydroxyethylcellulose, konjac glucomannan , pectin and chitosan) showed a pseudoplastic fluid behavior. Furthermore, the addition of CFG showed a remarkable viscosifying action for the aqueous solutions of these non-gelling polysaccharides likely due to their interaction by hydrogen bonding. However, the viscosity behavior of CFG with some gelling polysaccharides such as methylcellulose, gellan gum , carrageenan , xanthan, may be based on a different mechanism. A proper model of intermolecular interaction between CFG and these gelling and non-gelling polysaccharides has been discussed and proposed.
研究领域:
食品科学
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化学工程
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高分子科学
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Yilong Liu,Congyu Wu,Yaqin Liu,Yuanjiang Pan,Yining Pan,Xiaoying Li,Junwei Chen,Xian Li,Xiaoyong Zhao
A novel ionic liquid/inorganic salt system, [C 14 mim][PF 6 ]/Na 2 CO 3 , was developed for the simultaneous extraction and purification of polysaccharides from medicinal loquat leaves in a single-step procedure. The system forms a homogeneous phase upon heating in water for the polysaccharide extraction and transitions to a solid–liquid two-phase system upon cooling centrifugation for the polysaccharide separation, enabling polysaccharides to partition into the supernatant while proteins and other impurities gather in the sediment. This innovative approach demonstrated superior deproteinization efficiency, achieving a protein removal rate exceeding 99%, significantly outperforming the traditional Sevag method. Crude polysaccharides were further separated into alkali-extracted and water-soluble fractions, followed by purification using DEAE cellulose and Sephadex G-100 chromatography, yielding two homologous polysaccharides, EJp1-1 and EJp2-1. Based on the complementary information, mainly the NMR data, of these two homologous polysaccharides, a new strategy for rapid structure analysis was established. Both EJp1-1 and EJp2-1 were rhamnogalacturonan-I (RG-I) type pectins. EJp1-1 was primarily composed of the RG-I backbone, while EJp2-1 was dominated by the side chains. The side chains of EJp1-1 and EJp2-1 consisted primarily of α-1,5-linked arabinan and β-1,4-linked galactan, which were alternately attached to the O -4-position of rhamnose residues of the repeat RG unit of the main chain. To sum up, this work integrates the efficient extraction, purification and rapid structure analysis of homologous polysaccharides, potentially promoting the sustainable utilization of plant-derived resources.
研究领域:
植物化学
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天然产物化学
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药用植物研究
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Baojie Zhu,Wenxia Zhang,Shaoping Li,Jing Zhao
The polysaccharides from L. barbarum L. fruits (LBPs) are a kind of complex polysaccharides, which mainly contribute to the bioactivity of L. barbarum L. fruits and riches in acidic polysaccharides fractions. And the pharmacological research showed that the immunomodulatory effect of LBPs was closely related to α -1,4-D-galactosiduronic linkages. Therefore, as one of the main active polysaccharide fractions in LBPs, acidic polysaccharides fraction should also be used for the quality control of L. barbarum L. fruits. In this study, a novel quantitative evaluation strategy of polysaccharides from L. barbarum L. fruits based on simultaneous quantification of total polysaccharides and polysaccharides with active structure characteristics was proposed to improve their performance in the medicinal and functional food area more comprehensively and effectively, and beneficial to improve the quality control of L. barbarum L. fruits. The content of total polysaccharides in L. barbarum L. fruits was determined by HPSEC-RID method combined with universal dn / dc (0.15 mL·g −1 ). Additionally, HPAEC-PAD method combined with pectinase hydrolysis and m-hydroxybiphenyl colorimetric method were developed for the determination of acidic polysaccharide components related to active structural characteristics in L. barbarum fruits. The results revealed that the trends of total polysaccharides and acidic polysaccharides in L. barbarum L. fruits did not entirely coincide, further emphasizing the necessity of simultaneously quantifying both fractions for quality control. Furthermore, the m-hydroxybiphenyl method can be used as an alternative method to determine the content of acidic polysaccharides by HPAEC-PAD combined with pectinase hydrolysis.
研究领域:
药学
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食品科学
·
植物化学
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产品关联
DOI
高影响因子
Marine Drugs
·
2024
·Vol. 22
·期 6
·265
·
IF 4.9
Lige Cui,Huifang Sun,Xiaolei Shang,Jing Wen,Pengfei Li,Shengtao Yang,Linxia Chen,Xiangyang Huang,Haoyang Li,Ronghua Yin,Jinhua Zhao
Three polysaccharides (SnNG, SnFS and SnFG) were purified from the body wall ofStichopus naso. The physicochemical properties, including monosaccharide composition, molecular weight, sulfate content, and optical rotation, were analyzed, confirming that SnFS and SnFG are sulfated polysaccharides commonly found in sea cucumbers. The highly regular structure {3)-L-Fuc2S-(α1,}nof SnFS was determined via a detailed NMR analysis of its oxidative degradation product. By employing β-elimination depolymerization of SnFG, tri-, penta-, octa-, hendeca-, tetradeca-, and heptadeca-saccharides were obtained from the low-molecular-weight product. Their well-defined structures confirmed that SnFG possessed the backbone of {D-GalNAc4S6S-β(1,4)-D-GlcA}, and each GlcA residue was branched with Fuc2S4S. SnFS and SnFG are both structurally the simplest version of natural fucan sulfate and fucosylated glycosaminoglycan, facilitating the application of low-value sea cucumbersS. naso. Bioactivity assays showed that SnFG and its derived oligosaccharides exhibited potent anticoagulation and intrinsic factor Xase (iXase) inhibition. Moreover, a comparative analysis with the series of oligosaccharides solely branched with Fuc3S4Sshowed that in oligosaccharides with lower degrees of polymerization, such as octasaccharides, Fuc2S4Sled to a greater increase in APTT prolongation and iXase inhibition. As the degree of polymerization increases, the influence from the sulfation pattern diminishes, until it is overshadowed by the effects of molecular weight.
研究领域:
海洋生物化学
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药理学
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结构生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Xiaofei Liang,Mengqiu Liu,Anni Yao,Wanchen Cui,Yan Wei,Sheng Guo,Jialun Duan,Hongjie Kang,Xueyi Zhou,Shulan Su,Heiying Jin,Fang Zhang,Jin-ao Duan
In this study, the microbial fermentation characteristics and effects of neutral and acidic Lycii fructus polysaccharides (LFPs) on gut microbial composition were investigated using pure cultures of selected probiotic strains and mixed cultures of human fecal microflora in vitro . The results revealed that LFPs could significantly promote the growth of probiotics, with a faster proliferation rate in the early stages of fermentation. When LFPs were degraded and utilized by the gut microbiota, neutral arabinogalactans were more easily fermented, while acidic pectin polysaccharides exhibited a higher degree of anti-fermentation. LFPs were selectively modulated by microbiota; acidic polysaccharides promoted the growth of potentially beneficial bacteria, such as Lachnospira and Bacteroides vulgatus , while neutral polysaccharides significantly improved the relative abundances of Bifidobacterium longum and Bifidobacterium pseudocatenulatum . In summary, the results revealed that different types of LFPs regulate the gut microbiota in a structure-dependent manner, with uronic acid play a key role in selective regulation. This study aimed to better understand the fermentation characteristics and saccharide preferences of gut microbes for LFP structures and provide a theoretical basis for the relationship between the chemical structural and probiotic effects of LFPs.
研究领域:
微生物学
·
营养学
·
食品科学
·
胃肠病学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）