Huiqiao Liu,Jiakun Zhang,Fan Zhang,Jiale Yang,Yuyuan Lu,Jun-Tao Cao,Kangzhe Cao
Accurate detection of theophylline (THP) is often hindered by interference from structurally similar compounds and limitations in sensitivity. To address these challenges, this work introduces a novel voltage-assisted molecularly imprinted surface-enhanced Raman scattering (MIP-SERS) platform by integrating in-situ electrokinetic preconcentration with specific molecular recognition. The key innovation lies in applying an optimized potential during incubation to actively drive and accelerate THP binding kinetics at imprinted sites, thereby overcoming the intrinsic diffusion limitations of passive MIP sensors and improving the SERS intensity. As a result, the sensor achieves an exceptional detection limit of 0.52 nM, and a linear range of 1.0 × 10 −9 -1.0 × 10 −5 M, while demonstrating robust quantification in complex tea matrices. This platform has significant potential for precise therapeutic drug monitoring and food safety control, establishing a new paradigm for sensitive detection in complex samples.
Yanmin Shen,Wenju Liu,Chen Sun,TianTian Yao,Zehua Bao
The solid-liquid phase equilibrium solubility of Doxofylline was measured and discussed in ten pure organic solvents and co-solvent mixtures of acetonitrile + ethanol (+or water) at temperature range from 278.15 to 323.15 K. Results indicated that experimental mole fraction ( x ) of Doxofylline in ten neat solvents followed this order: acetonitrile > tetrahydrofuran > benzene > acetone > ethyl acetate > methanol > 1-propanol ≈ 2-propanol > ethanol ≈ water, and solubility increased with temperature increasing. Moreover, experimental solubility of Doxofylline firstly increased and then declined with the mass fraction increasing of acetonitrile in acetonitrile + ethanol (+or water) mixture solvents at each temperature. Hansen solubility parameter and solvent effect were used for explaining and discussing solubility rule of Doxofylline in selected solvents. Model correlation indicated the NRTL model was the most suitable than other models to predict solubility of Doxofylline in pure solvents and Sun model was better than other models in mixture solvents with accompanied by temperature and composition . Furthermore, thermodynamic properties of standard molar enthalpy, molar Gibbs free energy and molar entropy in solution was calculated and discussed.
Asthma is a chronic airway inflammation that is considered a serious public health concern worldwide. Nobiletin (5,6,7,8,3′,4′-hexamethyl flavonoid), an important compound isolated from several traditional Chinese medicines, especially Citri Reticulatae Pericarpium, is widely used for a number of indications, including cancer, allergic diseases, and chronic inflammation. However, the mechanism by which nobiletin exerts its anti-asthmatic effect remains unclear. In this research, we comprehensively demonstrated the anti-asthmatic effects of nobiletin in an animal model of asthma. It was found that nobiletin significantly reduced the levels of inflammatory cells and cytokines in mice and alleviated airway hyperresponsiveness. To explore the target of nobiletin, we identified PDE4B as the target of nobiletin through pharmacophore modeling, molecular docking, molecular dynamics simulation, SPR, and enzyme activity assays. Subsequently, it was found that nobiletin could activate the cAMP-PKA-CREB signaling pathway downstream of PDE4B in mouse lung tissues. Additionally, we studied the anti-inflammatory and anti-airway remodeling effects of nobiletin in LPS-induced RAW264.7 cells and TGF-β1-induced ASM cells, confirming the activation of the cAMP-PKA-CREB signaling pathway by nobiletin. Further validation in PDE4B-deficient RAW264.7 cells confirmed that the increase in cAMP levels induced by nobiletin depended on the inhibition of PDE4B. In conclusion, nobiletin exerts anti-asthmatic activity by targeting PDE4B and activating the cAMP-PKA-CREB signaling pathway.
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