JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2021
·Vol. 45
·期 10
·e15821
·
IF 2.19
Qun Huang,Hanliang Song,Yonggui Pan,Zhengke Zhang
Papaya fruit is easily affected by low temperature, causing chilling injury (CI). In this study, the exogenous arginine was applied in the storage of postharvest papaya fruit and it was found that 0.8 mM arginine treatment significantly alleviated CI of papaya fruit. Arginine treatment promoted the accumulation of polyamines (PAs), which might be ascribed to the increased activities of arginase, arginine decarboxylase (ADC), ornithine decarboxylase (ODC), and S-adenosylmethionine decarboxylase (SAMDC). A higher accumulation of proline was found in arginine-treated papaya fruit, which may be due to increased activity of ornithine-δ-aminotransferase (OAT) and Δ 1 -pyrroline-5-carboxylate acid synthase (P5CS) and inhibited activity of proline dehydrogenase (PDH). In addition, arginine treatment increased higher nitric oxide synthase (NOS) activity and promoted the generation of nitric oxide (NO). The results of this study indicate that exogenous arginine may improve the chilling tolerance of postharvest papaya fruit by regulating arginine and proline metabolism. Practical applications In recent years, chilling injury of tropical fruits is a significant problem, including papaya. Many studies on polyamine, proline, nitric oxide, and chilling injury of fruits, but arginine, as a common precursor of polyamine, proline, and nitric oxide, are rarely studied, especially in papaya fruit. Our findings demonstrated that the exogenous arginine can regulate endogenous arginine metabolism, induce polyamine, putrescine, and nitric oxide accumulation, and coordinate to alleviate chilling injury of papaya fruit.
研究领域:
植物生理学
·
园艺学
✓ 已验证
产品关联
DOI
高影响因子
Chinese Herbal Medicines
·
2025
·
IF 8.9
Weiwei Jiang,Xiaoyun Yan,Chenfei Lu,Xiaowei Xu,Enting Xu,Dandan Chen,Yuqiu Huang,Qingsong Shao,Aimin Lv
Objective Anoectochilus roxburghii is a valuable medicinal and ornamental plant. The aim of this study is to investigate the morphological and biochemical responses during the flower development stages of A. roxburghii , and to assess the effects of exogenous polyamines (PAs) on bud differentiation and metabolism, thereby providing a theoretical basis for understanding the flowering form and physiology of A. roxburghii . Methods In this study, morphological and biochemical responses in flower development stages of A. roxburghii were investigated using paraffin sections and stereomicroscope. A. roxburghii was divided into five periods, including vegetative growth period, flower bud period, flowering period, late flowering period and fruiting period. During the flowering phase, specific biochemical parameters were measured, including soluble sugar content, superoxide dismutase (SOD) activity, soluble protein content, peroxidase (POD) activity, and catalase (CAT) activity. These measurements were conducted to understand the biochemical changes occurring within A. roxburghii during its flowering process. Furthermore, the effects of PAs on bud differentiation were examined. Additionally, the activities of S-adenosylmethionine decarboxylase (SAMDC) and polyamine oxidase (PAO), as well as the content of polyphenols, polysaccharides, and flavonoids in A. roxburghii , were measured after PA treatment to evaluate the metabolic changes induced by exogenous PAs. Results During the flowering phase of A. roxburghii , soluble sugar content and SOD activity were steadily declining. Soluble protein content was initially increasing and then reducing, and POD and CAT activities showed opposite pattern. In addition, the effects of exogenous PAs on bud differentiation were investigated. Results showed that 3 mmol/L putrescine or 0.3 mmol/L spermidine significantly promoted the bud differentiation of A. roxburghii and advanced the flowering. The activities of SAMDC, PAO, and the content of polyphenols, polysaccharides and flavonoids in A. roxburghii significantly increased after PA treatment, demonstrating that exogenous PA can accelerate metabolism and improved the active ingredients content. Conclusion The flower development of A. roxburghii was divided into five stages, with significant changes in soluble sugar, protein, POD, SOD, CAT, MDA, and PRO levels. Exogenous putrescine and spermidine enhanced bud differentiation and accelerated flowering, increasing SAMDC and PAO activities, suggesting accelerated PA metabolism. PAs also improved active component content. These findings provide a theoretical basis for studying flower morphology and PA-induced flowering regulation of A. roxburghii .
研究领域:
植物学
·
药用植物学
·
生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2018
·
IF 2.718
Chen Geng,Tengfei Zhao,Chunxian Yang,Qiaozhuo Zhang,Feng Bai,Junlan Zeng,Fangyuan Zhang,Xiaoqiang Liu,Xiaozhong Lan,Min Chen,Zhihua Liao
N -methylputrescine is the precursor of nicotine and pharmaceutical tropane alkaloids such as hyoscyamine . Putrescine N -methyltransferase (PMT) catalyzes the N -methylation of putrescine to form N -methylputrescine. While the role of PMT in nicotine biosynthesis is clear, knowledge of PMT in the biosynthesis of tropane alkaloids (TAs) and the regulation of polyamines remains limited. We characterized a PMT gene from Hyoscyamus niger , designated HnPMT that was specifically expressed in roots, especially in the secondary roots and dramatically induced by methyl jasmonate (MeJA). The GUS gene was specifically expressed in Arabidopsis roots or in the vascular tissues , including pericycles and endodermis , of the H. niger hairy root cultures , when it was driven by the 5′-flanking promoter region of HnPMT . The recombinant HnPMT was purified for enzymatic assays. HnPMT converted putrescine to form N -methylputrescine, as confirmed by LC-MS. The kinetics analysis revealed that HnPMT had high affinity with putrescine but low catalytic activity, suggesting that it was a rate-limiting enzyme . When HnPMT was suppressed in the H. niger plants by using the VIGS approach, the contents of N -methylputrescine and hyoscyamine were markedly decreased, but the contents of putrescine, spermidine and a mixture of spermine and thermospermine were significantly increased; this suggested that HnPMT was involved in the biosynthesis of tropane alkaloids and played a competent role in regulating the biosynthesis of polyamines . Functional identification of HnPMT facilitated the understanding of TA biosynthesis and thus implied that the HnPMT-catalyzed step might be a target for metabolic engineering of the TA production in H. niger .
研究领域:
植物学
·
代谢工程
·
分子生物学
✓ 已验证
DOI
高影响因子
Toxics
·
2024
·Vol. 12
·期 8
·577
·
IF 3.9
Yi Kang,Cheng-Zhi Li,Abid Ullah,Qing Zhang,Xiao-Zhang Yu
Potential toxic element (PTE) pollution has emerged as a significant environmental and social concern in global agriculture. Chromium (Cr) occurs in different oxidation states naturally, among them Cr(VI), which is highly toxic. This study carried out biochemical and molecular tests to elucidate the accumulation of total soluble phenolics (TSPs) in rice plants exposed to Cr(VI) at 2.0, 8.0, and 16.0 mg Cr/L, emphasizing the interaction between polyamines (PAs) and abscisic acid (ABA). The results revealed significant Cr accumulation in different tissues of rice plants, which hindered their growth. Cr(VI) exposure increased the ABA concentration, with higher levels detected in the shoots than in the roots. The TSP concentration in rice tissues showed a positive relationship with the supplied concentrations of Cr(VI). The measured PAs, including spermine (Spm), putrescine (Put), and spermidine (Spd), exhibited varied responses to Cr(VI) stress, with only Spm concentration increasing with Cr(VI) concentrations. Real-time qRT-PCR showed PAs and ABA synthesis-associated genes such asOsADC1,OsAIH,OsCPA1, andOsCPA4were significantly up-regulated in shoot of rice plants treated with Cr(VI). These genes are associated with the second pathway of Put synthesis, originating from Arg. Almost all genes activated in the Met pathway were significantly up-regulated as well. Moreover, the genes involved in the interconversion among the three species of PAs exhibited completely different responses to Cr(VI) exposure. Overall, the biochemical analysis and gene expression data indicate that the interaction between ABA and Spm is likely to enhance the TSP levels in rice plants subjected to Cr(VI) toxicity.
研究领域:
植物学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Pei Yang,Ziqi Zhu,Minzhi Chen,Xiaoyan Zhou,Weimin Chen
A facile and straightforward microwave-assisted method was used to prepare polyamine-functionalized carbon dots (CDs) from a precursor comprising renewable xylan and branched polyethyleneimine (BPEI). The as-prepared BPEI-CDs were monodispersed sphere particles with an average diameter of about 8.62 nm, and exhibited excellent fluorescent property and high stability, as well as excitation-independent emission behavior. Furthermore, it is attractive that the BPEI-CDs can be used as novel fluorescent probes for detecting tannic acid (TA) sensitively and selectively. At the optimum condition, the TA detection system was established in water solution and ethanol solution with a dynamic range from 0.1 to 5 μM, and their detection limit of 36.8 nM and 44.9 nM were also determined, respectively. Most importantly, the BPEI-CDs-based sensors can be successfully applied to detect TA in real lake water and white wine samples, suggesting the low-cost and excellent BPEI-CDs are potential suitable for TA detection in practical application.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2022
·
IF 4.855
Cheng-yuan Feng,Hai-jun Chen,Meng-yao Yang,Zhe-sheng Feng,Yan Wang
An economical and efficient strategy for fabricating high-performance metallic copper layers on polyphenylene sulfide (PPS) for high-frequency microwave applications via mussel-inspired surface modification and electroless copper plating (ECP) is illustrated in this paper. Through UV-induced surface modification of catechol (CA)/polyamine (PA), the modified PPS substrate is capable of adsorbing silver particles, which provides activation sites for subsequent ECP. The impact of CA/PA modification on the relationship between PPS and the deposited copper layer can be determined by water contact angle measurements, X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and tape peel tests. The involvement of CA/PA has successfully introduced the functional groups for chemical bonding on the PPS surface, which greatly improves the adhesion between the PPS and the deposited copper layer. Moreover, after 40 min of ECP, the produced copper layer exhibits good adhesion at the 5B level (according to the ASTM D3559) and an excellent conductivity of 0.37 S/m (approx. 62% of bulk copper). Meanwhile, the deposited copper layer also has good resistance to acid, alkali, and salt corrosion (less than 25 g/m2 per unit area weight loss after 60 h of corrosion). This approach opens up an efficient path for the surface metallization of PPS, which has a broad potential for application in high-frequency copper-clad laminates (CCLs).
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-20
·
IF 14.7
Bi Guoshu,Liang Jiaqi,Bian Yunyi,Shan Guangyao,Huang Yiwei,Lu Tao,Zhang Huan,Jin Xing,Chen Zhencong,Zhao Mengnan,Fan Hong,Wang Qun,Gan Boyi,Zhan Cheng
Targeting ferroptosis, an iron-dependent form of regulated cell death triggered by the lethal overload of lipid peroxides, in cancer therapy is impeded by our limited understanding of the intersection of tumour’s metabolic feature and ferroptosis vulnerability. In the present study, arginine is identified as a ferroptotic promoter using a metabolites library. This effect is mainly achieved through arginine’s conversion to polyamines, which exerts their potent ferroptosis-promoting property in an H 2 O 2 -dependent manner. Notably, the expression of ornithine decarboxylase 1 (ODC1), the critical enzyme catalysing polyamine synthesis, is significantly activated by the ferroptosis signal——iron overload——through WNT/MYC signalling, as well as the subsequent elevated polyamine synthesis, thus forming a ferroptosis-iron overload-WNT/MYC-ODC1-polyamine-H 2 O 2 positive feedback loop that amplifies ferroptosis. Meanwhile, we notice that ferroptotic cells release enhanced polyamine-containing extracellular vesicles into the microenvironment, thereby further sensitizing neighbouring cells to ferroptosis and accelerating the “spread” of ferroptosis in the tumour region. Besides, polyamine supplementation also sensitizes cancer cells or xenograft tumours to radiotherapy or chemotherapy through inducing ferroptosis. Considering that cancer cells are often characterized by elevated intracellular polyamine pools, our results indicate that polyamine metabolism exposes a targetable vulnerability to ferroptosis and represents an exciting opportunity for therapeutic strategies for cancer.
研究领域:
肿瘤学
·
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Endocrinology
·
2025
·
IF 4.6
Cuiting Lin,Qiang Zheng,Haiyan Yu,Ting Wu,Lin Chen,Weihao Lin,Jianxin Pang,Yang Yang
Introduction: Maintaining polyamines homeostasis is essential for cardiovascular health, whereas elevated uric acid levels are recognized as a significant risk factor for the onset and progression of cardiovascular diseases. However, the interaction between uric acid and the regulation of polyamine homeostasis has not been extensively investigated. The objective of this study was to investigate the influence of uric acid on cardiac polyamines regulation and elucidate the role of polyamines in uric acid induced cardiomyocytic injury.Methods: The in vitro experiments utilized H9C2 cardiomyocytes, the hyperuricemic mouse model was established via potassium oxonate and hypoxanthine. Techniques included energy metabolomics, HPLC for polyamine quantification, qPCR, ELISA, immunofluorescence, and mitochondrial membrane potential assessment using JC-1 staining, MTT cell viability analysis. Results: Uric acid treatment can alter ornithine metabolism in cardiomyocytes, revealed a potential of shifting it from the traditional ornithine cycle towards the polyamine cycle. Both ODC1 and SAT1 protein levels were up-regulated in hyperuricemic mice indicated a dysorder of polyamines homostasis. A downregulation tendency of spermidine and spermine levels were observed in cardiomyocytes under uric acid treatment. Notably, exogenous supplementation with spermidine or spermine effectively mitigated the uric acid-induced decline in cardiomyocyte viability and mitochondrial membrane potential.Discussion: Uric acid disrupts polyamine homeostasis, leading to mitochondrial dysfunction and cardiomyocyte damage. Exogenous polyamine supplementation demonstrates therapeutic potential by preserving mitochondrial integrity. These findings unveil a potential mechanism underlying uric acid-induced cardiac injury and propose polyamine replenishment as a viable intervention strategy for hyperuricemia-related cardiovascular complications.
研究领域:
心血管生物学
·
代谢组学
·
分子医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Gut Microbes
·
2025
·
IF 11
Xinwei Li,Xia Xiao,Shengnan Wang,Biyu Wu,Yixuan Zhou,Pan Deng
Polyamines are important gut microbial metabolites known to affect host physiology, yet the mechanisms behind their microbial production remain incompletely understood. In this study, we developed a stable isotope-resolved metabolomic (SIRM) approach to track polyamine biosynthesis in the gut microbiome. Viable microbial cells were extracted from fresh human and mouse feces and incubated anaerobically with [U-13C]-labeled inulin (tracer). Liquid chromatography-high resolution mass spectrometry analysis revealed distinct 13C enrichment profiles for spermidine (SPD) and putrescine (PUT), indicating that the arginine-agmatine-SPD pathway contributes to SPD biosynthesis in addition to the well-known spermidine synthase pathway (PUT aminopropylation). Species differences were observed in the 13C enrichments of polyamines and related metabolites between the human and mouse microbiome. By analyzing the fecal metabolomics and metatranscriptomic data from an inflammatory bowel disease (IBD) cohort, we found significantly higher polyamine levels in IBD patients compared to healthy controls. Further investigations using single-strain SIRM and in silico analyses identified Bacteroides spp. as key contributors to polyamine biosynthesis, harboring essential genes for this process and potentially driving the upregulation of polyamines in IBD. Taken together, this study expands our understanding of polyamine biosynthesis in the gut microbiome and will facilitate the development of precision therapies to target polyamine-associated diseases.
研究领域:
微生物学
·
代谢组学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Zhongyang Wang,Muhammad Adnan Akram,Lihua Zhao,Zhiyu Liu,Q. Jason Niu
It is the goal of membrane science to continuously overcome the trade-off between permeability and selectivity of reverse osmosis (RO) membranes. In this study we propose a strategy based on the pore characteristics of cyclobutane-1,2,3,4-tetracarboxylic acid chloride (BTC) and trimesoyl chloride (TMC) crosslinked with cyclobutane-1,2,3,4-tetracarbohydrazide (CBTH) for constructing layered heterostructures of polyamide-hydrazide membranes. By using BTC and TMC successively, the porous BTC-CBTH polyamide was employed as the bottom layer, and the dense TMC-CBTH polyamide as the top layer, creating an RO membrane with a “sparse and dense” heterogeneous structure. This layered heterostructure ingeniously differentiates the structure and function of the selective layer of the RO membrane, enabling the loose bottom layer and the dense top layer to respectively perform the tasks of support and separation. The permeability coefficient of the prepared RO membrane reached 3.82 L⋅m −2 ⋅h −1 ⋅bar −1 , which was enhanced by 29% compared with that of the polyamide-hydrazide membrane prepared by single-step interfacial polymerization, while the rejection of NaCl was maintained at 99.13 %. The heterogeneous layered construction strategy effectively improves the permeability and selectivity of the RO membrane, and provides a universal solution for consistently overcoming the “trade-off” effect in RO membranes.
研究领域:
材料科学
·
环境工程
✓ 已验证
产品关联
DOI
高影响因子
FUEL
·
2017
·
IF 4.601
Wenbo Zhao,Qian Zhao,Zheng Zhang,Jiaju Liu,Rui Chen,Yuan Chen,Jian Chen
Acidic gas capture by traditional aqueous amine solution requires a plenty of energy for solvent regeneration. A phase-change capture system was considered to be a promising alternative because only the CO 2 -rich phase needs to be recovered and the CO 2 -lean phase can be reused directly without disposal, improving the energy utilization efficiency. Based on this principle, the phase-change absorption behavior of linear polyamines including ethylenediamine (EDA), diethylenetriamine (DETA), triethylenetetramine, tetraethylenepentamine, and cyclic diamines including piperazine (PZ) and triethylene diamine (DABCO) in organic solvents was investigated in the present work. The result indicated that polyamines could react with CO 2 to form carbamate precipitates except DABCO, which had no absorption effect. For the absorption of SO 2 , polyamines could react with SO 2 and water from air to form rare organic sulfites except DABCO, which merely absorbed two SO 2 molecules to form the electron transfer complex DABCO·(SO 2 ) 2 . The peculiar absorption behavior of DABCO for CO 2 and SO 2 implied that it is a highly selective phase-change desulfurizer for flue gas. The sequence of decomposition temperature for the CO 2 capture product was DETA-carbamate > EDA-carbamate > PZ-carbamate, while for the SO 2 capture product was [EDAH 2 ][SO 3 ] > [PZH 2 ][SO 3 ]·H 2 O > DABCO·(SO 2 ) 2 .
研究领域:
化学工程
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2022
·
IF 5.876
Yanyong Sun,Minmin Gou
Based on the one-dimensional tubular structure of halloysite nanotubes (HNTs), plant polyphenols and polyamines were used for in situ polymerization on the surface of HNTs to form amino-rich polymers, which were then removed as templates to synthesize polymer nanotubes. These nanotubes were then incorporated into a Pebax matrix to prepare mixed-matrix membranes (MMMs) for CO 2 /CH 4 gas separation. The removal of the HNT template increased the lumen diameter of the filler and significantly improved its gas permeability . And the amino groups on the inner surface of the polymer nanotubes were exposed. Further, the number of CO 2 reaction sites increased, which improved the reaction selectivity of CO 2 . In addition, the presence of amine groups on the inner and outer surfaces of the polymer nanotubes provided various transfer channels for CO 2 , which were conducive for CO 2 gas transfer in the membrane. When the CC-PEINT content was 8 wt%, the CO 2 permeability was 710 Barrer and CO 2 /CH 4 selectivity was 60. The CO 2 permeability and CO 2 /CH 4 selectivity of the Pebax/CC-PEINTs membranes increased by 163% and 132%, respectively, compared with those of the pure Pebax membrane.
研究领域:
材料科学
·
环境工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）