CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yiming Zhang,Zhi Chen,Songrui Wei,Jing Wang,Yujun Zhang,Huiling Lin,Hai Fu,Yingxia Liu,Qi Gao,Han Zhang,Zhongjian Xie
Simultaneous detection of multiple microRNAs (miRNAs) is crucial for diagnosing major diseases. Conventional miRNA detection methods face limitations in multiplexing, detection cycle, sensitivity and specificity. Herein, we present a novel CRISPR-linked amplification-free miRNA photoelectrochemical (CLAMP) biosensor for the direct and simultaneous analysis of multiple miRNAs with high sensitivity and specificity. The biosensor employs multi-channel screen-printed electrodes with vertical reduced graphene oxide as the substrate for the working electrode. In particular, based on the surface plasmon resonance effect of Au nanoparticles, ZnO/Au heterojunctions with an excellent photoelectric response are constructed and modified on the surface of vertical graphene to generate photocurrent signals. The probes containing protospacer adjacent motif sites, immobilised on the Au nanoparticle surface, enable the dCas9/sgRNA complex to act as a clamp to specifically recognise and bind target sequences. This biosensor translates miRNA recognition into the effect of the dCas9/sgRNA complex on the ultrahigh photoelectric response of ZnO/Au heterojunction to “CLAMP” the target miRNAs, with no need for reverse transcription or amplification. The biosensor achieves multi-channel miRNA detection and an amol-level detection limit while demonstrating single-base recognition specificity as well as excellent stability and reproducibility during the direct detection of miRNA-10b, miRNA-155 and miRNA-21. Clinical tests revealed higher miRNA concentrations in malignant pleurisy than in non-malignant pleurisy. The CLAMP biosensor presents a substantial advancement in miRNA detection and is a promising tool for clinical diagnostics and the comprehensive analysis of multiple miRNA targets.
研究领域:
生物技术
·
医学诊断
·
纳米材料科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2023
·
IF 17.1
Senyang Liu,Xiaohu Liu,Xueyu Zhu,Jinhua Yin,Jie Bao
Large-capacity information encryption has attracted significant interest in the information age. The diversity and controllability of spectra have positioned them to be widely applied for information encryption. Current spectra-based information encryption methods commonly rely on either spectral alteration induced by external stimuli or the utilization of narrowband channels within spectra. However, these methods encounter a common challenge in attaining both high security and large capacity simultaneously. To address these issues, we propose a multiple-channel information encryption system based on quantum dot (QD) absorption spectra. The diversity of QD absorption spectra and their broadband features ensure that the encrypted spectra can hardly be decrypted without knowing the correct channel matrix. Meanwhile, the large capacity is realized through the combination of multiple QD spectral channels with a theoretical maximum capacity of 24.0 bits in a single spectrum. In order to optimize the performance of our proposed system, the selection principle of the channel matrix is established to achieve the rapid identification of the optimal channel matrix in several milliseconds. The additivity of QD spectral channels and the consistency of QD spectra are also explored to minimize the impact of errors on information decryption. Furthermore, two spectral encryption scenarios of spatial pattern and spectral pattern are applied to demonstrate the feasibility, showcasing their ability to achieve both a high level of security and large capacity. Owing to the advantages offered by QD spectra, the QD spectra-based information system exhibits excellent potential for broader applications in information storage, authentication, and computing.
研究领域:
光学
·
信息科学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 9
·1-9
·
IF 5.3
Song Yiwei,Jin Xiuyan,Zhao Yiou,Cheng Shuwen,Xu Sai,Bu Shengjun,Liu Liming,Zhou Chunyang,Pang Chunying
Single-level biomarker detection has the limitation of insufficient accuracy in cancer diagnosis. Therefore, the strategy of developing highly sensitive, multi-channel biosensors for high-throughput ctDNA determination is critical to improve the accuracy of early diagnosis of clinical tumors. Herein, in order to achieve efficient detection of up to ten targets for early diagnosis of ovarian cancer, a DNA-nanoswitch-based multi-channel (DNA-NSMC) biosensor was built based on the multi-module catalytic hairpin assembly-mediated signal amplification (CHA) and toehold-mediated DNA strand displacement (TDSD) reaction. Only two different fluorescence signals were used as outputs, combined with modular segmentation strategy of DNA-nanoswitch-based reaction platform; the multi-channel detection of up to ten targets was successfully achieved for the first time. The experimental results suggest that the proposed biosensor is a promising tool for simultaneously detecting multiple biomarkers for the early diagnosis of ovarian cancer, offering new strategies for the early screening, diagnosis, and treatment not only for ovarian cancer but also for other cancers. Graphical abstract
研究领域:
分子生物学
·
医学诊断
·
生物传感技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2021
·
IF 8.742
Jingyuan Guan,Xinda You,Benbing Shi,Yanan Liu,Jinqiu Yuan,Chao Yang,Xiao Pang,Hong Wu,Jianliang Shen,Chunyang Fan,Mengying Long,Runnan Zhang,Zhongyi Jiang
Graphene oxide (GO) membranes hold great promise in molecular separation owing to the ultrathin thickness as well as precisely controllable interlayer distance . However, the tortuous mass transport pathway along stacked nanosheets hinders the availability of high-flux GO membranes. Herein, we design multi-pathway GO membranes to afford fast water transport through in-plane and interlayer pathways. The in-plane pathway is introduced by the porous GO (pGO) nanosheets and the interlayer pathway is enlarged by intercalating hydrophilic halloysite nanotubes (HNTs) into adjacent pGO nanosheets , jointly contributing to the enhanced water permeance. Through manipulating the intercalator amount, the optimized membrane displays ultrahigh water permeance of 206.7 L m −2 h −1 bar −1 and dye rejections over 98.5%. Furthermore, the resultant membranes exhibit resistance against broad-spectrum oil-in-water emulsions. Our study can pave a facile and prospective way to fabricating high-performance two-dimensional laminated membranes.
研究领域:
材料科学
·
环境工程
✓ 已验证
DOI
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2018
·Vol. 24
·期 18
·4703-4709
·
IF 5.16
Li'na Qiao,Sihua Qian,Yuhui Wang,Shifeng Yan,Hengwei Lin
Graphical A “lab-on-a-nanoparticle” approach for the detection and discrimination of antibiotics has been developed. The sensing platform is designed on the basis of the different channel's fluorescence recoveries of full-color emissive carbon dots and metal ion ensembles with the addition of antibiotics. Moreover, the proposed method can be successfully employed for the quantitative identification of antibiotic residues in real food samples. Fluorescent carbon dots (CDs) have received considerable attention in recent years due to their superior optical properties. To take further advantages of these unique features, herein, a CDs-based “lab-on-a-nanoparticle” approach for the detection and discrimination of antibiotics is developed. The sensing platform was designed based on the different channel's fluorescence recoveries or further quenching of the full-color emissive CDs (F-CDs) and metal ion ensembles upon the addition of antibiotics. The F-CDs exhibited unusually comparable emission intensity nearly across the entire visible spectrum even as the excitation wavelength is shifted, making it very suitable for the construction of multi-channel sensing systems. The sensing platform was fabricated on the basis of the competing interaction of metal ions with the F-CDs and antibiotics. Three metal ions (i.e., Cu 2+ , Ce 3+ and Eu 3+ ) can efficiently quench the fluorescence of the F-CDs. Upon the addition of antibiotics, the fluorescent intensities either recovered at different emission wavelengths or were further quenched to various degrees. The fluorescence response patterns at different emission wavelength were characteristic for each antibiotic and can be quantitatively differentiated by standard statistical methods (e.g., hierarchical clustering analysis and principal component analysis). Moreover, as an example, the proposed method was applied for quantitative detection of oxytetracycline with a limit of detection to be 0.06 μ m . Finally, the sensing system was successfully employed for residual antibiotics detection and identification in real food samples.
研究领域:
分析化学
·
纳米技术
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Wen-Qi Cui,Xi-Ya Shan,Wan-Jun Cai,Xing-Hai Zhou,Yuan-Lin Yan,Min-Yu Li,Yong-Fang Qian,Yuan Gao,Li-Hua Lyu,Shang-Ru Zhai,Hong-Zhu Liu,Zhong-Gang Wang
An ingenious microstructure of electromagnetic microwave absorption materials is crucial to achieve strong absorption and a broad bandwidth. Herein, one-dimensional (1D) carbon fibers with implantation of zero-dimensional (0D) ZIF-8-derived carbon frameworks and construction of a three-dimensional (3D) microcosmic multichannel porous structure are fabricated by electro-blown spinning, solvent-thermal reaction, and high-temperature pyrolysis techniques. The 1D carbon fiber skeleton with a multichannel structure provides a direct axial conductive pathway for charge transport, which plays an important role in dielectric loss. The 0D surface carbon frameworks offer plenty of heterogeneous interfaces to trigger intensive interfacial polarization loss and act as dihedral angles for microwave scattering. The 3D microcosmic multichannel pores can not only generate multiple reflections as much as possible to dissipate electromagnetic microwave energy but also supply huge interior cavities to improve impedance matching. Thanks to the synergistic effect of a strong electrically conductive pathway for enhancing the conductive loss, a plenteous heterogeneous interface for triggering intensive interfacial polarization loss, microcosmic multichannel pores for generating multiple reflections and improving impedance matching, and N and O atom doping for inducing dipole polarization, the optimal sample with an ingenious microstructure delivers an excellent absorption performance of a minimum reflection loss of −35.5 dB at a thickness of 5.0 mm and an effective absorption bandwidth of 6.72 GHz (10.96–17.68 GHz) at a thickness of 2.0 mm. Such a well-designed multichannel porous carbon fiber may pave the way for the exploitation of high-performance microwave absorbing materials.
研究领域:
材料科学
·
电子工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Yuquan Xiao,Pei Cheng,Xiaohua Zhu,Maotian Xu,Meiling Liu,Haitao Li,Youyu Zhang,Shouzhuo Yao
Urinary tract infections (UTIs) have greatly affected human health in recent years. Accurate and rapid diagnosis of UTIs can enable a more effective treatment. Herein, we developed a multichannel sensor array for efficient identification of bacteria based on three antimicrobial agents (vancomycin, lysozyme, and bacitracin) functional gold nanoclusters (AuNCs). In this sensor, the fluorescence intensity of the three AuNCs was quenched to varying degrees by the bacterial species, providing a unique fingerprint for different bacteria. With this sensing platform, seven pathogenic bacteria, different concentrations of the same bacteria, and even bacterial mixtures were successfully differentiated. Furthermore, UTIs can be accurately identified with our sensors in ∼30 min with 100% classification accuracy. The proposed sensing systems offer a rapid, high-throughput, and reliable sensing platform for the diagnosis of UTIs.
研究领域:
生物医学工程
·
分析化学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2018
·Vol. 30
·期 6
·065502
·
IF 3.404
Feng Zhang,Xiaojie Wang,Hui Tang,Xu Jie,Xuemei Jiang,Weili Wei
Fatty acids (FAs) are important dietary sources of fuel for animals and structural components for cells. The number, position and configuration of olefins in the alkyl chains play important roles in the impacts of FAs on human health. Currently, structural profiling of FAs in edible oils and fats is an important issue in nutrition industries and food safety. Due to the lack of distinct functional groups, it is extremely difficult to discriminate FAs with structural differences by facile and in situ sensing methods. A few chemosensors have been developed for shape selective sensing of FAs, but their capability and performance were still limited. Herein, for the first time, we proposed a multichannel Au nanosensor for visual and pattern-generating inspection of FAs based on the highly selective binding ability of Ag+ to olefinic bonds and Ag+ regulable color variation of Au nanoparticles. As a result, the nanosensor showed good selectivity for five FAs with subtle structural difference as low as 5 nM. By further deriving three channel signals in respect of color and color depth, a signature-like signal pattern could be generated by principal component analysis for each FA and even different FA mixtures such as edible oils. Hence, structural variation of FAs in edible hot pot oils with heat treatment was successfully monitored by this Au nanosensor over time. This sensor holds great promise in point-of-care inspection of edible oils and fats.
研究领域:
分析化学
·
食品科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 51
·29455-29463
·
IF 2.936
Hai-Zhen Zhang,Zhen-Liang Xu,Jing-Ying Sun
Nanofiltration (NF) membranes with simultaneous high rejection of divalent cations and anions and high water permeation were designed and fabricated via interfacial polymerization (IP) on three-channel capillary ultrafiltration (UF) membranes. MWCNTs-COOH were modified with poly(amidoamine) (PAMAM) and the as-synthesized MWCNTs-PAMAM were embedded into the inner polyamide skin-layer of the NF membranes by incorporating them into a piperazine (PIP) aqueous solution, followed by IP with trimesoyl chloride (TMC). The rigid MWCNTs and the dendrimer PAMAM molecules endow the as-fabricated NF membranes with high porosity and good hydrophilicity. Additionally, the –NH2 groups of PAMAM introduce some positive sites into the polyamide layer. The as-prepared NF membranes with incorporated MWCNTs-PAMAM exhibit a pure water flux of 48.7 L m−2 h−1 and 92.6% and 88.5% rejection for Na2SO4 and MgCl2, respectively, at 4 bar. Moreover, the NF membranes display high rejection for sulfates and metal cations, including heavy metal ions. The practicability of the membranes for mine-wastewater treatment was tested, and the membranes showed above 80% rejection of heavy metals and solution flux of about 30 L m−2 h−1. In addition, their separation performance and stability were satisfactory during the long-term run. The high rejection of the membranes for metal cations is ascribed to the positive sites offered by MWCNTs-PAMAM and the narrow membrane pores since both electrostatic repulsion and size exclusion play a role during membrane filtration. The good separation performance of the membranes for multivalent anions and heavy metal cations illustrates their potential for applications in heavy metal wastewater treatment.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Yuanyuan He,Yongbo Wang,Gennian Mao,Chengyuan Liang,Min Fan
Owing to the high risk to human and environmental health , heavy metal pollution has become a global problem. Rapid, accurate and multiplexed determination of heavy metal ions is critical. In this work, we reported a promising approach to designing ratiometric fluorescent nanoprobes for multiplexed determination of Hg 2+ , Cu 2+ , and Ag + ions. The nanoprobes (CDs-QDx) were designed by mixing the CDs and multicolor CdTe QDs without the involvement of recognition elements. The CDs were insensitive to heavy metal ions while CdTe QDs showed the size-dependent fluorescence response to different heavy metal ions, thereby establishing a ratiometric detection scheme by measuring the fluorescence intensity ratios of CDs-QDx systems. By evaluating the detection performance, the CDs-QDx (x = 570, 650, and 702) were successfully used for differentiation and quantification of Hg 2+ , Cu 2+ , and Ag + ions. In addition, we also carried out the detection of heavy metal ions in actual samples with acceptable results. We believed that this work offers new insight into the design of ratiometric fluorescent nanoprobe for multiplexed determination of not only heavy metals but also some other analytes by combining the CDs with CdTe QDs with fine-tuned sizes.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2022
·
IF 24.319
Lili Guo,Jingqi Chi,Jiawei Zhu,Tong Cui,Jianping Lai,Lei Wang
Seawater electrolysis is an efficient method for producing carbon-neutral hydrogen; however, it is hindered by high energy cost and chlorine evolution reaction. In this study, we report Ru, P dual-doped NiMoO 4 multichannel nanorods in-situ grown on nickel foam (Ru/P-NiMoO 4 @NF), which can achieve chlorine-free hydrogen production by coupling seawater splitting with thermodynamically favorable urea oxidation . The Ru/P-NiMoO 4 @NF exhibits bifunctional activity with working potentials of 0.23 V to deliver 3000 mA cm −2 for HER and 1.46 V to deliver 1000 mA cm −2 for UOR. The overall urea splitting system in the two-electrode electrolyzer require low voltage of 1.73 V to drive 500 mA cm −2 , and demonstrate remarkable durability to keep above 100 mA cm −2 for 145 h. Density functional theory calculations reveal that dual-doping modulate the d -band center of catalyst, thus enhancing the adsorption of reactants and intermediates. This work provides information for designing catalysts for combing seawater splitting with urea purification.
研究领域:
材料科学
·
电催化
·
可再生能源
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2021
·
IF 15.881
Xin Ye,Jiafeng Ruan,Yuepeng Pang,Junhe Yang,Yongfeng Liu,Yizhong Huang,Shiyou Zheng
Room-temperature sodium–sulfur (RT Na–S) batteries are widely considered as one of the alternative energy-storage systems with low cost and high energy density. However, the both poor cycle stability and capacity are two critical issues arising from low conversion kinetics and sodium polysulfides (NaPSs) dissolution for sulfur cathodes during the charge/discharge process. Herein, we report a highly stable RT Na–S battery cathode via building heterostructures in multichannel carbon fibers. The TiN-TiO2@MCCFs, fabricated by electrospinning and nitriding techniques, are loaded with the active material S, forming S/TiN-TiO2@MCCFs as the cathode in a RT Na–S battery. At 0.1 A g–1, the cathode produces the capacity of more than 640 mAh g–1 within 100 cycles with a high Coulombic efficiency of nearly 100%. Even at 5 A g–1, the battery still exhibites a capacity of 257.1 mAh g–1 after 1000 cycles. Combining structural and electrochemical analyses with the first-principles calculations reveals that the incorporation of the highly electrocatalytic activity of TiN with the powerful chemisorption of TiO2 well stabilizes S and also alleviates the shuttle effects of polysulfides. This work with simple processes and low cost is expected to promote the further development and application of metal–S batteries.
研究领域:
材料科学
·
电化学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）