Pharmaceuticals
·
2020
·Vol. 13
·期 4
·55
·
IF 4.286
Gui-Lin Chen,Fredrick Munyao Mutie,Yong-Bing Xu,Flora Didii Saleri,Guang-Wan Hu,Ming-Quan Guo
Rhamnus prinoidesL’Herit (R. prinoides) has long been widely consumed as folk medicine in Kenya and other Africa countries. Previous studies indicated that polyphenols were abundant in genusRhamnusand exhibited outstanding antioxidant and anti-inflammatory activities. However, there are very few studies on such pharmacological activities and the polyphenol profile of this plant up to now. In the present study, the antioxidant activities of the crudeR. prinoidesextracts (CRE) and the semi-purifiedR. prinoidesextracts (SPRE) of polyphenol enriched fractions were evaluated to show the strong radical scavenging effects against 1,1-diphenyl-2- picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) (0.510 ± 0.046 and 0.204 ± 0.005, mg/mL), and 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) (0.596 ± 0.005 and 0.096 ± 0.004, mg/mL), respectively. Later, the SPRE with higher contents of polyphenols and flavonoids displayed obvious anti-inflammatory activities through reducing the NO production at the dosage of 11.11 − 100 μg/mL, and the COX-2 inhibitory activity with an IC50value at 20.61 ± 0.13 μg/mL. Meanwhile, the HPLC-UV/ESI-MS/MS analysis of polyphenol profile ofR. prinoidesrevealed that flavonoids and their glycosides were the major ingredients, and potentially responsible for its strong antioxidant and anti-inflammatory activities. For the first time, the present study comprehensively demonstrated the chemical profile ofR. prinoides, as well as noteworthy antioxidant and anti-inflammatory activities, which confirmed thatR. prinoidesis a good natural source of polyphenols and flavonoids, and provided valuable information on this medicinal plant as folk medicine and with good potential for future healthcare practice.Keywords:Rhamnus prinoides;polyphenols;antioxidant;anti-inflammatory;chemical profile
研究领域:
药理学
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植物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2013
·Vol. 15
·期 6
·1-11
·
IF 2.175
Jiang Xinde,Sun Daohua,Zhang Genlei,He Ning,Liu Hongyu,Huang Jiale,Odoom-Wubah Tareque,Li Qingbiao
The effects of different biomolecules in Artocarpus heterophyllus Lam leaf extract on the morphology of obtained gold nanoparticles were investigated in this study. The results indicated that reducing sugars, flavones, and polyphenols consisting of about 79.8 % dry weight of the leaf extract were mainly involved in providing the dual function of reduction and the size/shape control during the biosynthesis. The gold nanoparticles present included 64 ± 10 nm nanospheres, 131 ± 18 nm nanoflowers, and 347 ± 136 nm (edge length) nanoplates and they were synthesized using the main content of reducing sugars, flavones, and polyphenols, respectively, after they were desorbed by the AB-8 macroporous adsorption resin column. Particularly, flower-like and triangular/hexagonal gold nanoparticles with a yield more than 80 % were obtained. Possible shape-directed agents for the nucleation and growth were characterized by FTIR, it can be seen that ketones were bound on the surface of the spherical and flower-like GNPs, while both the ketones and carbonyls bound on the Au {111} plane this may have favored the formation of the twin defects, which are very essential for nanoplates’ formation.
研究领域:
植物学
·
纳米生物学
·
材料科学
✓ 已验证
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2016
·
IF 3.449
Lu Zhang,Zong-cai Tu,Xing Xie,Yu Lu,Zhen-xing Wang,Hui Wang,Xiao-mei Sha
This research aimed to compare the antihyperglycemic and antioxidant abilities, and to characterize the bioactive constituents of two Acer palmatum cultivars, Acer palmatum ‘ Atropurpureum ’ (APA) and Acer palmatum Thunb (APT). Dry materials were respectively extracted with 70% and 90% methanol, followed by sequentially fractionation with ethyl acetate and n -butanol. The phytochemical profile of selected fraction was determined by using UPLC-QTOF-MS/MS. APT is a better source of natural antioxidants and α- glucosidase inhibitors than APA due to its higher total phenolic content (TPC), and stronger antioxidant and α- glucosidase inhibitory activities. The highest total phenolic and total flavonoid content were found in the ethyl acetate fraction (EAF) of 90% methanol extract of APT (468.49 mg GAE/g Extract) and 70% methanol extract of APA (142.60 mg QuE/g Extract), respectively. The EAF of 70% methanol extract of APT gave the strongest DPPH· scavenging ability, ABTS + · scavenging ability and α- glucosidase inhibitory, with the IC 50 values of 7.06, 4.18 and 1.99 μg/mL, respectively. The latter two were much higher than that of individual positive control. HPLC analysis revealed similar phytochemical profiles of all EAFs. Twenty-five compounds, including 3 organic acids, 5 phenolic acids, 8 tannins, 7 flavonoids and 2 proanthocyanidins, were identified or tentatively identified from APT, with tannins and flavonoid glucosides as the major active compounds. The results indicated a strong potential of Acer palmatum as a natural source of excellent antioxidants and antihyperglycemic compounds in the prevention and treatment of type II diabetes and its complication.
研究领域:
植物学
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药理学
·
代谢疾病研究
✓ 已验证
产品关联
DOI
高影响因子
Biomed Research International
·
2021
·
IF 3.411
Wengkun Li,BingMin Wu,Yange Wang,Ying Lin,Lin An,Guifang Zhang
Stahlianthus involucratus (S. involucratus) has anti-inflammatory, antinociceptive, and antipyretic activities; however, there are no literature reports on its antioxidant capacity. This study presents a comparative assessment of the polyphenols contents, flavonoids contents, and antioxidant activity of the aqueous and methanol extracts of S. involucratus (ASI and MSI). Moreover, the expression of oxidative stress-related genes in H2O2-induced H9c2 cells pretreated with the MSI was measured by RT-qPCR, and furthermore, MSI were characterized by UHPLC-Q-Orbitrap-MS/MS. The results indicated that the MSI had higher antioxidant contents and antioxidant capacity, and MSI could inhibit H2O2-induced oxidative stress in H9c2 cells by activating the Nrf2/HO-1 pathway. UHPLC-Q-Orbitrap-MS/MS characterized 15 phenolic compounds from the MSI. In conclusion, S. involucratus has the potential antioxidant capacity.
研究领域:
植物学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2022
·
IF 5.318
Yi-Long Ma,Yue Wang,Zheng-Fang Wu,Jie Mei,Wen-Qing Zhang,Ya-Fang Shang,Kiran Thakur,Zhao-Jun Wei
To assess the effect of the digestive system on the phenolics and antioxidant capacity of Lycium barbarum fruit, its extract was digested and analyzed in vitro . The results revealed that the phenolics/flavonoids contents and antioxidant capacity in the pH-treated group and digested group generally decreased by 4.39%–21.22% in the oral and intestinal digestions but increased by 1.26%–16.14% in the gastric digestion. The phenolic content and antioxidant capacity were highly correlated in digested groups ( r > 0.899, p < 0.01). Five main phenolics including rutin , p -coumaric acid and its hexoside derivatives were identified and quantified, and these phenolics displayed higher stability during the digestion, implying that they may be the potential functional phenolics of L. barbarum fruit.
研究领域:
食品科学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
·
2025
·
IF 2
Xuelin Chen,Hanlei Wang,Kun Zhang,Jing Lu,Yumei Zhang
For the phytochemical studies of the fruits of Kochia scoparia (L.) Schrad. (Kochiae Fructus, KF), we isolated chemical components from KF. Twenty compounds, including six alkaloids ( 1 – 6 ), nine polyphenols ( 7 – 15 ), three flavonoids ( 16 – 18 ), and two sterols ( 19 – 20 ) were isolated and identified from the ethanol extract of KF by chromatographic and spectroscopic techniques. Compounds 2 , 3 , and 5 – 16 were isolated from KF and Kochia genus for the first time. Furthermore, by discussing the taxonomic significance of these secondary metabolites, we found that Araliaceae, Compositae, Ranunculaceae, and Leguminosae might have a close evolutionary relationship. In conclusion, these results provide new insights into the chemical diversity and chemotaxonomic significance of K. scoparia in the Kochia genus and the Chenopodiaceae family.
✓ 已验证
产品关联
DOI
高影响因子
Antioxidants
·
2023
·Vol. 12
·期 7
·1475
·
IF 7
Ding-Tao Wu,Wen Deng,Jie Li,Jin-Lei Geng,Yi-Chen Hu,Liang Zou,Yi Liu,Hong-Yan Liu,Ren-You Gan
Fruit thinning is a common practice employed to enhance the quality and yield of kiwifruits during the growing period, and about 30–50% of unripe kiwifruits will be thinned and discarded. In fact, these unripe kiwifruits are rich in nutrients and bioactive compounds. Nevertheless, the applications of thinned young kiwifruits and related bioactive compounds in the food and functional food industry are still limited. Therefore, to promote the potential applications of thinned young kiwifruits as value-added health products, the extraction, characterization, and evaluation of beneficial effects of phenolic compounds from thinned young fruits of red-fleshedActinidia chinensiscv ‘HY’ were examined in the present study. A green and efficient ultrasound-assisted deep eutectic solvent extraction (UADE) method for extracting phenolic compounds from thinned young kiwifruits was established. A maximum yield (105.37 ± 1.2 mg GAE/g DW) of total phenolics extracted from thinned young kiwifruits by UADE was obtained, which was significantly higher than those of conventional organic solvent extraction (CSE, about 14.51 ± 0.26 mg GAE/g DW) and ultrasound-assisted ethanol extraction (UAEE, about 43.85 ± 1.17 mg GAE/g DW). In addition, 29 compounds, e.g., gallic acid, chlorogenic acid, neochlorogenic acid, catechin, epicatechin, procyanidin B1, procyanidin B2, quercetin-3-rhamnoside, and quercetin-3-O-glucoside, were identified in the kiwifruit extract by UPLC-MS/MS. Furthermore, the contents of major phenolic compounds in different kiwifruit extracts prepared by conventional organic solvent extraction (EE), ultrasound-assisted ethanol extraction (UEE), and ultrasound-assisted deep eutectic solvent extraction (UDE) were compared by HPLC analysis. Results revealed that the content of major phenolics in UDE (about 15.067 mg/g DW) was significantly higher than that in EE (about 2.218 mg/g DW) and UEE (about 6.122 mg/g DW), suggesting that the UADE method was more efficient for extracting polyphenolics from thinned young kiwifruits. In addition, compared with EE and UEE, UDE exhibited much higher antioxidant and anti-inflammatory effects as well as inhibitory effects against α-glucosidase and pancreatic lipase, which were closely associated with its higher content of phenolic compounds. Collectively, the findings suggest that the UADE method can be applied as an efficient technique for the preparation of bioactive polyphenolics from thinned young kiwifruits, and the thinned young fruits of red-fleshedA. chinensiscv ‘HY’ have good potential to be developed and utilized as functional foods and nutraceuticals.Keywords:unripe kiwifruit;deep eutectic solvent;extraction optimization;polyphenolic;antioxidant activity;anti-inflammatory activity
研究领域:
食品科学
·
植物化学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Liang Xu,Da-Wen Sun,You Tian,Zhiwei Zhu
Polyphenols and enzymes usually suffer from degradation during transportation due to the lack of a cold chain system in developing countries and regions. In this study, anthocyanin and trypsin were selected as examples of polyphenols and enzymes and investigated for minimizing their degradation during ambient transportation using hydrogel packaging with combined evaporative and daytime radiative cooling functions. A polyacrylamide/polyvinyl alcohol contained with nanoparticles (NPs@PAAm/PVA) hydrogel packaging was thus developed. The NPs@PAAm/PVA packaging exhibited desirable swelling behaviour, high solar reflectance, and strong atmospheric emissivity to synergistically achieve evaporative and daytime radiative cooling. The indoor experiments indicated that the vial with NPs@PAAm/PVA packaging realised sub-ambient temperatures under different working temperatures and humidities, and the field tests indicated that the vial with NPs@PAAm/PVA packaging could effectively preserve the anthocyanin and trypsin without degradation caused by strong sunlight and high temperature. Consequently, the NPs@PAAm/PVA packaging with evaporative and daytime radiative cooling effects has promising prospects for anthocyanin and trypsin transportation in an energy-saving and sustainable manner.
研究领域:
食品科学
·
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD QUALITY
·
2020
·
IF 1.763
Ashok Biswas,Susmita Dey,Defang Li,Yi Liu,Jiangjiang Zhang,Siqi Huang,Gen Pan,Yong Deng
In addition to fiber, Corchorus (jute) leaf is also rich in secondary metabolites and is used in folk medicine in jute-producing communities. It has been reported that jute halts progression of and helps manage different chronic diseases like tumors, obesity, diabetes, and cardiovascular diseases. Various phytochemical, mineral, and antioxidant potency properties of 30 genotypes belonging to Corchorus capsularis and Corchorus olitorius were evaluated in the current study. The results demonstrate that the range of total flavonoids and polyphenols was 3.04 to 13.66 mg rutin equivalent (RE)/g and 5.12 to 7.78 mg gallic acid equivalent (GAE)/g DW, respectively. Total tannin and saponin content in both species was 13.08 to 26.95 mg tannic acid (TAE)/g and 34.45 to 114.59 mg tea saponin (TSE)/g DW, respectively, when analyzed for the first time. Moreover, this study sincerely establishes that jute leaf is a great source of mineral elements (magnesium, zinc, and selenium) and could also be a good energy source. The antioxidant properties of samples were examined with three unique strategies, including DPPH, FRAP, and ABTS values of leaf extract ranging from 206.42 to 351.77 μg/ml (IC50), 16.69 to 94.69 mmol Fe (II)/g DW, and 50.27 to 149.90 mmol trolox equivalent (TE)/g DW, respectively. A principle component analysis (PCA) explained 58.52% of the variance, while a hierarchical cluster analysis (HCA) was performed to construct five distinct groups based on their secondary compound metabolites content and antioxidant activities. Therefore, this study facilitates selection of the high genotypes that might be used as new materials for developing industrial and medicinal uses in addition to sorting genotypes for future genetic engineering purposes in order to enhance a particular bioactive compound and its natural antioxidants that are beneficial for human health.
研究领域:
植物学
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药理学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Ruimin Wang,Lu Wang,Lin Zhang,Sitong Wan,Congfa Li,Sixin Liu
This study focused on the relationship between content levels of phytochemicals and the biological activities of noni ( Morinda Citrifolia L.) fruit extracts (NFEs) prepared with traditional solvents and deep eutectic solvents (DESs). The results indicated the total phenolic content in Bet-Gly (Betaine: Glycerol) extracts (11.89 mg GAE/g DW) and total iridoid content in 70% ethanol extracts (26.38 mg CE/g DW) were the highest. A total of 17 compounds were identified and quantified in NFEs. Traditional solvent extracts, except ethyl acetate, exhibited higher antioxidant activities than DESs. Three DES extracts showed higher activities against pancreatic lipase than traditional solvent extracts. Multivariate analysis revealed that the type of extraction solvent exerts a significant influence on the phytochemical compositions and biological activities of NFEs. This study provided valuable information on the efficient extraction of phytochemicals from noni fruits and DESs are promising green solvent for the extraction of bioactive compounds from noni fruits.
研究领域:
植物化学
·
药理学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Wan-Jun Long,Hai-Long Wu,Tong Wang,Ming-Yue Dong,Ru-Qin Yu
The quantification of polyphenols in propolis has attracted considerable concerns for their biological properties. Liquid chromatography–mass spectrometry (LC–MS) coupled with chemometrics multivariate calibration analytical strategy was developed for simple and rapid determination of eleven polyphenols in Chinese propolis. The eleven polyphenols were fast eluted out within 7.0 min, and then they were detected by mass spectrometry operated in the full scan mode. The acquired second-order data of LC–MS were resolved by two well-known chemometrics tools including alternating trilinear decomposition (ATLD) and multivariate curve resolution-alternating least squares (MCR-ALS). With the aid of the distinctive “second-order advantage”, satisfactory resolution and reliable results were obtained in the presence of chromatographic shifts, serious co-eluted peaks and diverse unknown interferences. The mean value of spiked recoveries obtained by the ATLD and MCR-ALS methods for eleven polyphenols in propolis samples were in the range of 81.2–109.1% and 75.8–107.1%, respectively, which have verified by the LC–MS/MS method. Statistical parameters and analytical figures of merit were also used to assess the performances of the proposed strategy. All these results demonstrated that the proposed strategy opened a new avenue for quantification of the targeted polyphenols in complex propolis samples for quality monitoring, which does not require toilsome sample pretreatments, elaborate experimental conditions and more advanced high cost instrumentations.
研究领域:
分析化学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Changting Yin,Shanjie Han,Xiaoguo Fang,Chunfeng Jin,Baoyu Han,Mengxin Wang
Xiaoqinggan black tea is made by filling black tea into fresh citrus peel with the pulp removed, followed by sunlighting and low-temperature roasting. However, existing studies have not yet systematically analyzed the chemical basis of flavor characteristics and in vitro antioxidant activity of Xiaoqinggan black tea. In this study, a systematically analysis of Xiaoqinggan black tea, including its raw materials (black tea and citrus peel) and constituent parts (tea core and outer peel), were conducted by quantifying the functional components through high performance liquid chromatography (HPLC) and chemometrics, in combination with the electronic tongue and colorimetry assays. Additionally, the in vitro antioxidant activity of Xiaoqinggan black tea and its related samples was also evaluated through five different assays. The multivariate analysis revealed that 30 differential compounds were identified, demonstrating their key role in distinguishing the five sample groups. Notably, three polyphenols (catechin gallate, taxifolin, kaempferol), six amino acids (L-theanine, L-tyrosine, L-valine, L-methionine, L-leucine, l -lysine), and total free amino acids served as the dominant factors in unique flavor of Xiaoqinggan black tea through the correlation analysis of electronic tongue and differential compounds. Chromatic aberration analysis revealed that the tea infusion of Xiaoqinggan black tea was more vividly red and bright, and it exhibited a more intense flavor. The combined analysis of antioxidative activity and functional compounds suggested polyphenols were the main contributors to the high total antioxidant activity of Xiaoqinggan black tea. In conclusion, polyphenols and amino acids constituted the unique flavor and chromatic characteristics of Xiaoqinggan black tea, and provided an important chemical basis for its antioxidant activity. This study provides insights for improving tea processing and product quality, making it relevant to both the tea industry and scientific research.
研究领域:
食品化学
·
分析化学
·
营养学
·
植物科学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）