BIOCHEMICAL ENGINEERING JOURNAL
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2021
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IF 3.978
Yi Zhang,Xihui Kang,Feng Zhen,Zhongming Wang,Xiaoying Kong,Yongming Sun
Enzymatic hydrolysis is emerging as a promising unconventional pretreatment method for disrupting microalgae cells. However, the sustainability of biological enzyme needs to be improved due to low efficiency of enzymatic hydrolysis with single enzyme. Cellulase , xylanase , pectinase , snailase, lysozyme and trypsin were selected to pretreat microalgae Scenedesmus sp. for lipid extraction. The results shown that the pretreatment of a multi-enzyme combination of cellulase, xylanase and pectinase perform a better enzymatic hydrolysis effect than each single enzyme. Results shown that different sequences of enzymatic hydrolysis had a significant effect on the lipid extraction performance from microalgae, the lipid recovery yield of 100% (total lipids content) could be achieved with the sequence of stepwise enzymatic hydrolysis: pectinase (10 mg g -1 dry weight microalgae), cellulase (20 mg g -1 ) and xylanase (14 mg g -1 ). The SEM image, FTIR measurement, HPLC and XPS analysis indicated that the outer layer of trilaminar sheaths in the microaglal cell walls was first degraded by pectinase, and then cellulose and hemicellulose in the inner layer were destroyed by cellulase and xylanase. This study provides an efficient and environmental-friendly route for lipid extraction as a new strategy of enzymatic hydrolysis to valorize high-value added products in microalgae downstream processing .
研究领域:
生物技术
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可再生能源
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DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
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2023
·Vol. 46
·期 9
·1365-1373
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IF 3.8
Wang Ru,Jia Jianyao,Liu Xue,Chen Yaru,Xu Qing,Xue Feng
Chiral compounds are important drug intermediates that play a critical role in human life. Herein, we report a facile method to prepare multi-enzyme nano-devices with high catalytic activity and stability. The self-assemble molecular binders SpyCatcher and SpyTag were fused with leucine dehydrogenase and glucose dehydrogenase to produce sc-LeuDH (SpyCatcher-fused leucine dehydrogenase) and GDH-st (SpyTag-fused glucose dehydrogenase), respectively. After assembling, the cross-linked enzymes LeuDH-GDH were formed. The crosslinking enzyme has good pH stability and temperature stability. The coenzyme cycle constant of LeuDH-GDH was always higher than that of free double enzymes. The yield of l - tert -leucine synthesis by LeuDH-GDH was 0.47 times higher than that by free LeuDH and GDH. To further improve the enzyme performance, the cross-linked LeuDH-GDH was immobilized on zeolite imidazolate framework-8 (ZIF-8) via bionic mineralization, forming LeuDH-GDH @ZIF-8. The created co-immobilized enzymes showed even better pH stability and temperature stability than the cross-linked enzymes, and LeuDH-GDH@ZIF-8 retains 70% relative conversion rate in the first four reuses. In addition, the yield of LeuDH-GDH@ZIF-8 was 0.62 times higher than that of LeuDH-GDH, and 1.38 times higher than that of free double enzyme system. This work provides a novel method for developing multi-enzyme nano-device, and the ease of operation of this method is appealing for the construction of other multi-enzymes @MOF systems for the applications in the kinds of complex environment. Graphical abstract
研究领域:
生物化学
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合成生物学
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材料科学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Donghui Zhao,Yunhao Deng,Junyi Shi,Xinye Ni,Chaoqing Li,Yang Bai,Yang Xuan,Jianhao Wang
Nanozymes with multienzyme activity for reactive oxygen species (ROS) generation and intracellular redox imbalance are attractive strategy for cancer therapy. However, it is severely limited by low biocompatibility and catalytic efficiency, hypoxic and high levels of GSH in the tumor microenvironment. To address these issues, a copper doping carbon nanozyme (CC) with multienzyme activity was designed and integrated with photosensitizer Ce6 and gelatin to fabricate ROS amplifier (CCC). Gelatin endowed CCC with good biocompatibility, low hemolysis, and enzyme responsive degradation. CCC with high CAT-like, POD-like, OXD-like, and GSHox-like activities can induce the intracellular ROS storm formation to eliminate the cancer cells. The OXD-like activity and PDT performance mediated 1 O 2 generation was markedly potentiated by the CAT-like activity of CCC via catalyzing high expression of H 2 O 2 to generate O 2 . At the same time, a large amount of ·OH were produced through POD-like activity of CCC and GSH was depleted by the GSHox-like activities of CCC, resulting in a destructive ROS storm formation and cellular redox homeostasis disruption. Both in vivo and in vitro experiments showed that CCC displayed satisfactory anti-tumor activity and biocompatibility. Our work provides a novel strategy for the development of nanozyne enhanced photodynamic therapy of cancer.
研究领域:
生物医学工程
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肿瘤学
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材料科学
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DOI
PubMed
高影响因子
GREEN CHEMISTRY
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2024
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IF 9.3
Daocheng Liao,Shiming Tang,Ying Lin,Suiping Zheng
1,3-Propanediamine is a diamine widely used in microelectronics and porous materials. It can also be used as a curing agent for epoxy resin, forming valuable three-dimensional structures, or as a raw material for synthesizing the radiation-resistant drug amifostine. However, traditional chemical synthesis methods for producing 1,3-propanediamine often contradict the 12 principles of green chemistry. In this study, we designed a new pathway for producing 1,3-diaminopropane through a retrosynthetic-designed multi-enzyme cascade from crude glycerol in vitro. Compared with traditional chemical methods, this multienzyme cascade is simpler in process, less energy-consuming, cleaner in substrate usage, and more environmentally friendly. In the cascade reaction, we selected glycerol dehydratase (KpGDHT) to dehydrate the intermediate product 3-amino-1,2-propanediol based on substrate promiscuity. We then modified the substrate preference of KpGDHT to obtain the optimized mutant KpGDHT/Q337A/S302D, which exhibited a 4.4-fold increase in activity toward 3-amino-1,2-propanediol compared with the wild-type KpGDHT. Using KpGDHT/Q337A/S302D as the catalyst, 3.8 mM 1,3-diaminopropane was produced from 5 mM glycerol within 8h under optimal conditions, achieving 76% conversion efficiency. Finally, we evaluated the performance of 1,3-diaminopropane synthesized from crude glycerol and obtained 1,3-diaminopropane with a concentration of 3.95 mM, which is comparable to that from pure glycerol. Given its robustness and environmental benefits, this proposed cascade reaction pathway has great potential to produce 1,3-diaminopropane on an industrial scale.
研究领域:
生物化学
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合成生物学
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环境科学
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产品关联
DOI
高影响因子
ACS Catalysis
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2025
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IF 13.1
Shihao Li,Jiafu Shi,Yu Chen,Xinyu Mao,Shusong Liu,Yang Yang,Hong Wu,Zhongyi Jiang
Amino alcohols are widely used in chemical and pharmaceutical industries, while conventional synthesis methods face drawbacks such as low selectivity and high energy consumption. Multienzyme catalysis combined with light-driven cofactor regeneration affords a platform of light-driven synthesis of chemicals, where the transfer of electrons and protons as well as the route of enzymatic reactions dominates the energy conversion efficiency and atomic economy of carbon utilization. Herein, we construct a light-driven synthesis system comprising an energy-supplying module and a multienzyme catalytic module for 2-aminoethanol synthesis from formaldehyde and ammonia. First, a S-scheme heterojunction photocatalysts with a metal–organic framework core and a bipyridine-based conjugated polymer shell are prepared as an energy-supplying module. Through synergistic intensification of proton transfer and electron transfer, the photocatalysts render cofactor (NADH) regeneration efficiency with a turnover frequency of 30.49 h–1. Second, a multienzyme catalytic module is constructed for 2-aminoethanol synthesis in the presence of NADH. Under optimal conditions, this module produces 2-aminoethanol with an average reaction rate of 76.34 μmol L–1 h–1 within 24 h. Finally, the energy-supplying module is coupled with the multienzyme catalytic module for continuous synthesis of 2-aminoethanol from formaldehyde and ammonia, generating 0.55 mmol L–1 2-aminoethanol after 4 light–dark cycles. In addition, triethanolamine (TEOA) and glucose, a biomass-derived carbon source, are both confirmed as effective electron donors for NADH regeneration and light-driven synthesis system. This work opens a pathway for the synthesis of amino alcohols and demonstrates a paradigm for the intensification of reaction processes.
研究领域:
生物化学
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绿色化学
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DOI
高影响因子
Science China-Chemistry
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2021
·Vol. 65
·期 1
·153-161
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IF 9.445
Shen Saiji,Wu Chu,Shang Yinghui,Shen Hongdou,Liao Yue,Guo Yuting,Hu Min,Wang Xia,Li Gang,Wang Qigang
There are several natural materials which have evolved functional gradients, ingeniously attaining maximal efficacy from limited components. Herein, we utilized the spatiotemporal distribution of initiator acetylacetone to regulate the multienzyme polymerization and fabricate a chitosan-polymer hydrogel. The temporal priority order of acetylacetone was higher than phenol-modified chitosan by density functional theory calculation. The acetylacetone within the gelatin could gradually diffuse spatially into the chitosan hydrogel to fabricate the composite hydrogel with gradient network structure. The gradient hydrogel possessed a transferring topography from the two-dimensional pattern. A continuously decreased modulus along with acetylacetone diffusion was confirmed by atomic force microscope-based force mapping experiment. The water-retaining ability of various regions was confirmed by low-field nuclear magnetic resonance (NMR) and thermogravimetric analysis (TG) analysis, which led to the spontaneous actuation of gradient hydrogel with maximum 1821°/h curling speed and 227° curling angle. Consequently, the promising gradient hydrogels could be applied as intelligent actuators and flexible robots.
研究领域:
材料科学
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生物化学
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智能材料
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产品关联
DOI
高影响因子
ACS Catalysis
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2025
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IF 13.1
Mengkai Hu,Teng Bao,Zhen Qin,Qiang Wang,Hengwei Zhang,Yujue Wang,Jiajia You,Zhenglian Xue,Rongzhen Zhang,Shang-Tian Yang,Xian Zhang,Zhiming Rao
Surface-displayed whole-cell biocatalysis offers significant advantages over traditional intracellular enzyme-based methods but faces challenges in complex multienzyme processes and low efficiency. We present Scaffold-Mediated Assembly Regulation Tool (SMART), a programmable platform for dynamic control of multienzyme biocatalysts on Corynebacterium glutamicum surfaces. SMART integrates orthogonal cross-linked scaffolds with the twin Catcher/Tag pairs (SpyCatcher/SpyTag and SnoopCatcher/SnoopTag), controllable molecular ratios, and high loading capacity, offering user-defined functionality for enzyme assembly. We optimized SMART through computational modeling and experimental validation and created bifunctional scaffolds and long tandem repeats for optimal enzyme loading. The versatility of SMART was demonstrated by codisplaying in a two enzyme sequential cascade with tunable stoichiometry, significantly improving the conversion of maltodextrin to trehalose. Furthermore, we integrated a Bxb1 recombinase-based state machine (RSM) genetic circuit, allowing temporal regulation of enzyme and scaffold expression within a single cell. Finally, the system’s applicability was showcased in isomaltulose production from low-cost sucrose. SMART design represents a significant advancement in surface-displayed biocatalysis, offering a programmable platform for complex multienzyme reactions with potential applications in various industrially relevant biocatalysts.
研究领域:
合成生物学
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生物化学
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代谢工程
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DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2020
·Vol. 55
·期 9
·3182-3189
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IF 2.773
Zhang Ye,Xing-He Tan,Zhi-Wei Liu,Rana Muhammad Aadil,Yi-Cheng Tan,Muhammad Inam-ur-Raheem
Summary The robust cell wall structure of Haematococcus pluvialis ( H. pluvialis ) consists of polysaccharides and tough non-hydrolysable sporopollenins, which makes it difficult to extract superpotent antioxidant, astaxanthin from these cells. Therefore, breakdown of cell wall is a key step in the overall process of astaxanthin recovery. In this study, the mechanism of three well-established chemical techniques for cell disruption of H. pluvialis cysts [ionic liquids (IL), hydrochloric acid (HCl) and multiple enzymes (multi-enzyme, ME)] on deconstruction of the cyst cell wall of H. pluvialis was explored and characterised by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), nuclear magnetic resonance (NMR) and gas chromatography–mass spectrometry (GC-MS) analyses. The results demonstrated that the three cell wall breakdown techniques exhibited high extraction efficacies for the recovery of astaxanthin from H. pluvialis [IL (86.71 ± 2.06%), HCl (80.52 ± 2.28%) and ME (71.08 ± 2.49%)]. However, their performances on disrupting the trilayered cell walls of H. pluvialis were significantly different, which were confirmed by distinct morphologies of the treated cell walls visualised by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Meanwhile, the results of FTIR confirmed that, to some extent, cellulose, hemicellulose and lignin in the cell walls were hydrolysed by HCl, IL and ME treatments. However, ME exhibited a less hydrolytic effect on lignin than HCl and IL. Moreover, XRD and NMR analyses implied that the amorphous region of cell wall was susceptible to hydrolysis/breakdown by the three techniques.
研究领域:
生物化学
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植物学
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分析化学
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DOI
高影响因子
Journal of Environmental Sciences
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2025
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IF 6.3
Mei Du,Xianghao Zha,Jingzhang Liu,Mengfan Niu,Maoyong Song,Guibin Jiang
Biomimetic nanozymes opens up new opportunities for sensitive, rapid and field detection of organophosphorus pesticides (OPs). However, it still remains challenges in how to improve the sensitivity and stability of biomimetic nanozymes under harsh conditions. Herein, we synthesized a novel biomimetic nanozyme composed of hemin and bovine serum albumin (BSA) in the nanopores of poly-L-lysine methacryloyl (PLMA) inverse opal hydrogel (PLMA-Hemin-BSA). PLMA-Hemin-BSA achieves superior peroxidase-like activity and shows high stability due to the confinement effect. A multi-enzyme cascade reaction was constructed for the colorimetric detection of five widely used OPs by integrating PLMA-Hemin-BSA with natural choline oxidase and acetylcholinesterase. The detection limits for dichlorvos, chlorpyrifos, paraoxon, methamidophos, and parathion were as low as 0.024, 0.073, 0.12, 0.56, and 1.4 ng/mL, respectively. More importantly, the average recovery rates and the relative standard deviations (RSD) of chlorpyrifos in paddy water, soil and wheat samples were 86.62 %-100.13 % and 2.08 %-8.65 %, which meet the standard of the International Union of Pure and Applied Chemistry (IUPAC, recoveries of 70 %-120 % with RSD < 20 %). This study represented advanced methods toward enhancing the activity and stability of biomimetic nanozymes via spatial nanopores-assisted strategy.
研究领域:
分析化学
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环境科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
CARBON
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2025
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IF 11.6
Zhenlong He,Yanli Yang,Ruijiao Li,Qian He
Rheumatoid arthritis is a chronic autoimmune disorder characterized by the pathological accumulation of reactive oxygen species (ROS), which contributes to sustained inflammation and tissue damage. Current therapeutic modalities frequently exhibit low rates of clinical remission, and effectively mitigating the oxidative stress microenvironment associated with excessive ROS presents a significant challenge. Herein, we present a novel carbon dots (CDs) with multi-enzyme activity, featuring a porphyrin iron core and surface functional groups rich in phosphorus and oxygen, providing abundant catalytic sites for enhanced scavenging of hydrogen peroxide, hydroxyl radicals, and superoxide anions. Furthermore, the phosphate groups on the surface of CDs exhibited a specific affinity for hydroxyapatite, thereby facilitating targeted delivery to the bone. The localized accumulation of CDs with multi-enzyme activity at inflammatory lesions can effectively reduce oxidative stress levels, subsequently inhibiting bone destruction in collagen-induced arthritis rat models and significantly alleviating disease symptoms. This study integrates multiple catalytic sites and targeting moieties into a single nanostructure, representing a promising strategy for the precise treatment of oxidative stress-related bone pathologies.
研究领域:
生物医学
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纳米医学
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免疫学
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产品关联
DOI
高影响因子
TEXTILE RESEARCH JOURNAL
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2021
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IF 1.82
Taotao Qiang,Yadong Pu,Qi Zhang
Down fiber – a natural and environmentally recyclable insulation material – is applied mainly in the area of natural-filled products. However, owing to its natural deficiencies of low fluffiness and thermal stability, large-scale application is limited. In our work, an enzyme preparation (transglutaminase (TGase)) was used as a fluffy agent to improve the fluffy degree of down. With fluffy as the main index, the single factor condition of only using TGase enzyme was optimized, and fluffiness was increased by 22%. On the basis of this optimum condition, the compound dosage of papain and TGase was further optimized. The fluffy degree of down was improved by hydrolysis of papain and crosslinking of TGase. Amino group content, thermogravimetry (TG), differential scanning calorimetry (DSC), moisture absorption, tensile strength, and elongation at break of down treated with different enzyme reagents were tested. The results showed that the thermal stability of down fiber treated with the multi enzyme increased significantly, the denaturation temperature was increased from 53.7°C to 77.4°C, and the moisture absorption was also improved. The elastic property also exhibited a great enhancement; elongation at break increased from 12.4% to 37.7%, nearly three times higher than the original property, and the tensile strength increased from 180 MPa to 370 MPa, almost 2.1 times as much as before, increased by 105.6%.
研究领域:
生物化学
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材料科学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2014
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IF 3.107
Renliang Huang,Ming Cao,Hong Guo,Wei Qi,Rongxin Su,Zhimin He
Pomelo peel is an abundant pectin-rich biomass waste in China and has the potential to serve as a source of fuels and chemicals. This study reports a promising way to deal with pomelo peel waste and to utilize it as raw material for ethanol production via simultaneous saccharification and fermentation (SSF). An integrated strategy, incorporating hydrothermal treatment, multienzyme formulation, and fed-batch operation, was further developed to enhance the ethanol production. The results show that hydrothermal treatment (120 °C, 15 min) could significantly reduce the use of cellulase (from 7 to 3.8 FPU g–1) and pectinase (from 20 to 10 U g–1). A multienzyme complex, which consists of cellulase, pectinase, β-glucosidase, and xylanase, was also proven to be effective to improve the hydrolysis of pretreated pomelo peel, leading to higher concentrations of fermentative sugars (36 vs 14 g L–1) and galacturonic acid (23 vs 9 g L–1) than those with the use of a single enzyme. Furthermore, to increase the final ethanol concentration, fed-batch operation by adding fresh substrate was employed in the SSF process. A final solid loading of 25% (w/v), which is achieved by adding 15% fresh substrate to the SSF system at an initial solid loading of 10%, produced 36 g L–1 ethanol product in good yield (73.5%). The ethanol concentration is about 1.73-fold that at the maximum solid loading of 14% for batch operation, whereas both of them have a closed ethanol yield. The results indicate that the use of the fed-batch mode could alleviate the decrease in ethanol yield at high solid loading, which is caused by significant mass transfer limitation and increased inhibition of toxic compounds in the SSF process. The integrated strategy demonstrated in this work could open a new avenue for dealing with pectin-rich biomass wastes and utilization of the wastes to produce ethanol.
研究领域:
生物技术
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环境科学
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能源科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）