Applied Materials Today
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2018
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IF 0
Yansen Wang,Cai Wang,Longxue Qiao,Jingxuan Feng,Yudong Zheng,Yong Chao,Wei He,Yajie Xie,Wanjun Shuai,Minglu Li
Effective bleeding control is becoming more and more important in the military field and civilian trauma arena. However, traditional hemostats present limitations, they have been proven to be less useful for irregular, deep or penetrating wounds with uncontrollable hemorrhage caused by minor-caliber weapons, shrapnel or explosive devices. Here, a series of porous polyvinyl alcohol (PVA)/alginate composite foams (PACFs) were prepared through crosslinking reaction and lyophilization process. The effect of different content of alginate on the physicochemical properties and hemostatic function of the PACF were evaluated. The results demonstrated that PACF could not only rapidly absorb plasma, but also stimulate blood cells, thus further promoting blood coagulation. In addition, the PACF with higher alginate content showed increased capability to enhance thrombus generation, blood clotting, and blood cells adhesion and aggregation. More importantly, the PACF exhibited over 2000% volume expansion, high flexibility and shape-adaptive ability, and could therefore expand to entirely fill and conform to the shape of wound cavity. The in vivo hemostatic tests further demonstrate the high hemostatic efficiency of PACF. These results suggest that PACF has strong potential as a hemostatic material to be applied for massive hemorrhage control and deep/irregular wounds treatment.
研究领域:
生物材料学
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创伤医学
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军事医学
✓ 已验证
产品关联
DOI
ACS Applied Bio Materials
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2021
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IF 0
Wen Liu,Xiao Yang,Pengpeng Li,Feng Sang,Lina Cao,Bingxu Zhang,Zhizhen Meng,Zhaipu Ma,Changcan Shi
Uncontrollable hemorrhage, especially uncompressible and massive hemorrhage, is life threatening. To develop a kind of hemostatic material that is biocompatible and has effective hemostatic performance, a starch-polyethylene glycol sponge (St-PEG sponge) incorporated with electrospraying microspheres made from carboxymethyl chitosan and sodium alginate (CMC/SA eMPs) is employed to fabricate the topical hemostatic agent for application. To load thrombin safely and effectively, CMC/SA eMPs encapsulating thrombin compounds (eMPs@Thr) are further incorporated with St-PEG sponge to obtain eMPs@Thr/sponge. The results show that eMPs@Thr/sponge could obviously reduce blood loss and shorten the hemostatis time compared with commercial hemostatic products Kuai Kang. The construction of the eMPs@Thr/sponge could not only maintain the expandable properties of the St-PEG sponge but also strengthen the procoagulant activity. Therefore, this work provides a facile approach for loading thrombin given the fact that thrombin suffers from instability and risk of thrombus. It is predicted that eMPs@Thr/sponge might hold great potential in uncompressible and massive hemorrhage control.
研究领域:
生物材料
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止血
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生物医学工程
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组织工程
✓ 已验证
产品关联
DOI
PubMed
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Zhitao Ren,Mengya Li,Fang Wang,Jialu Qiao,Mǎdǎlina Georgiana Albu Kaya,Keyong Tang
Uncontrollable acute bleeding and wound infection pose significant challenges in emergency treatment and surgical operations. Therefore, the research and development of highly efficient antibacterial hemostatic agents are of great importance in reducing the mortality rate among patients with massive hemorrhage. In this study, we utilized hydrophobically modified chitosan (HM-CS) and gallic acid chitosan (GA-CS) to create a composite sponge (HM/GA-CS) that exhibits complementary advantages. The composite sponge combines the alkyl chain and polyphenol structure, allowing it to adsorb blood cells and plasma proteins simultaneously. This synergistic effect was confirmed through various tests, including blood cell adhesion, plasma protein barrier behavior, and in vitro hemostatic testing. Furthermore, experiments conducted on a rat liver injury model demonstrated that the composite sponge achieved rapid coagulation within 52 s, resulting in significantly lower bleeding volume compared with traditional gauze. In addition, the incorporation of GA-CS into HM-CS enhanced the antibacterial properties of the composite sponge. The antibacterial rate of the composite sponge against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) reached 100 % and 98.2 %, respectively. To evaluate its biocompatibility, the composite sponge underwent blood compatibility and cell activity tests, confirming its suitability. The HM/GA-CS sponge holds promising applications in managing cases of massive hemorrhage.
研究领域:
生物材料
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止血
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抗菌剂
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生物医学工程
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伤口愈合
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2024
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IF 10.7
Chengkun Liu,Zi Li,Lili Liu,Xianfeng Qu,Zhuang Shi,Zhidong Ma,Xiaoqiang Wang,Fang Huang
Uncontrolled hemorrhage stands as the primary cause of potentially preventable deaths following traumatic injuries in both civilian and military populations. Addressing this critical medical need requires the development of a hemostatic material with rapid hemostatic performance and biosafety. This work describes the engineering of a chitosan-based cryogel construct using thermo-assisted cross-linking with α-ketoglutaric acid after freeze-drying. The resulting cryogel exhibited a highly interconnected macro-porous structure with low thermal conductivity, exceptional mechanical properties, and great fluid absorption capacity. Notably, assessments using rabbit whole blood in vitro, as well as rat liver volume defect and femoral artery injury models simulating severe bleeding, showed the remarkable hemostatic performance of the chitosan cryogel. Among the cryogel variants with different chitosan molecular weights, the 150 kDa one demonstrated superior hemostatic efficacy, reducing blood loss and hemostasis time by approximately 73 % and 63 % in the hepatic model, and by around 60 % and 68 %, in the femoral artery model. Additionally, comprehensive in vitro and in vivo evaluations underscored the good biocompatibility of the chitosan cryogel. Taken together, these results strongly indicate that the designed chitosan cryogel configuration holds significant potential as a safe and rapid hemostatic material for managing severe hemorrhage.
研究领域:
材料科学
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生物材料
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止血
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创伤医学
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2024
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IF 10.7
Yu-He Zhu,Chen-Yu Zhou,Xu Peng,Wei Wang,Zhuang Liu,Rui Xie,Da-Wei Pan,Xiao-Jie Ju,Liang-Yin Chu
Developing a hemostatic material suitable for rapid hemostasis remains a challenge. This study presents a novel aminated gelatin sponge cross-linked with dialdehyde starch, exhibiting excellent biocompatibility and hemostatic ability. This aminated gelatin sponge features hydrophilic surface and rich porous structure with a porosity of up to 80 %. The results show that the aminated gelatin sponges exhibit superior liquid absorption capacity and can absorb up to 30–50 times their own mass of simulated body fluid within 5 min. Compared with the commercial gelatin hemostatic sponge and non-aminated gelatin hemostatic sponge, the aminated gelatin hemostatic sponge can accelerate the hemostatic process through electrostatic interactions, demonstrating superior hemostatic performance in both in vitro and in vivo hemostasis tests. The aminated gelatin sponge can effectively control the hemostatic time within 80 s in the in vivo rat femoral artery injury model, significantly outperforming both commercial and non-aminated gelatin sponges. In addition, the aminated gelatin sponge also exhibits good biocompatibility and certain antibacterial properties. The proposed aminated gelatin sponge has very good application prospects for the management of massive hemorrhage.
研究领域:
生物材料
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止血
·
生物医学工程
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组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Bioactive Materials
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2021
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IF 14.593
Yansen Wang,Yifan Zhao,Longxue Qiao,Faxing Zou,Yajie Xie,Yudong Zheng,Yong Chao,Ying Yang,Wei He,Siming Yang
Uncontrollable hemorrhage leads to high mortality and thus effective bleeding control becomes increasingly important in the military field and civilian trauma arena. However, current hemostats not only present limitation when treating major bleeding, but also have various side effects. Here we report a self-expanding porous composites (CMCP) based on novel carboxymethyl cellulose (CMC) fibers and acetalized polyvinyl alcohol (PVA) for lethal hemorrhage control. The CMC fibers with uniform fibrous structure, high liquid absorption and procoagulant ability, are evenly interspersed inside the composite matrix. The obtained composites possess unique fiber-porous network, excellent absorption capacity, fast liquid-triggered self-expanding ability and robust fatigue resistance, and their physicochemical performance can be fine-tuned through varying the CMC content. In vitro tests show that the porous composite exhibits strong blood clotting ability, high adhesion to blood cells and protein, and the ability to activate platelet and the coagulation system. In vivo hemostatic evaluation further confirms that the CMCP presents high hemostatic efficacy and multiple hemostatic effects in swine femoral artery major hemorrhage model. Additionally, the CMCP will not fall off from the injury site, and is also easy to surgically remove from the wound cavity after the hemostasis. Importantly, results of CT tomography and 3D reconstruction indicate that CMCP can achieve shape adaptation to the surrounding tissues and the wound cavities with different depths and shapes, to accelerate hemostasis while protecting wound tissue and preventing infection.
研究领域:
生物材料
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止血
·
组织工程
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生物医学工程
·
创伤护理
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）