JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2024
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IF 2.8
Xiaoxue Xu,Sheng Guo,Jiangyan Chen,Yuhan Song,Xueli Wu,Feng Liu,Jin-Ao Duan
The Zingiberis Rhizoma − Jujubae Fructus herb pair (ZJHP) is a classic herb pair in traditional Chinese medicine. The herb pair shows the effect of dispelling cold, harmonizing the middle and improving gastrointestinal function, and is widely used for patients with stomach cold syndrome (SCS), stomachache and anemofrigid cold. The gingerols, shogaols, flavonoids and triterpenic acids are the important bioactive ingredients of ZJHP. However, few pharmacokinetic studies have been investigated in vivo for the above compounds. To comprehend the kinetics of active components and promote their curative application, a fast and sensitive ultra-high performance liquid chromatography coupled with mass spectrometry (UHPLC-MS/MS) method was established for simultaneous determination of 12 analytes in normal and SCS rats in this study. The results showed that the pharmacokinetic parameters (C max , T max , t 1/2z , MRT 0-t , AUC 0-t and AUC 0-∞ ) in SCS model were significantly different from those in normal rats. In addition, the pharmacokinetics of rats given ZJHP were also varied from single herb oral administration, especially in model condition. These results indicated that the in vivo processes of the above analytes changed under pathological conditions and the compatibility of the herb pair could significantly influence the absorption of active components, which might provide an insight and further supports for the clinical application of ZJHP.
研究领域:
药理学
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中药学
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
PHYTOMEDICINE
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2024
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IF 6.7
Shi-Kang Zhou,Jin-Di Xu,Xiao-Qin Gao,Ren-Jie Zhang,Fang-Fang Cheng,Wei-Feng Yao,Yi Zhang,Ting Geng,Li Zhang
Background Shizao decoction (SZD) consisted of Euphorbia kansui (EK), Euphorbia pekinensis (EP), Daphne genkwa (DG), and Fructus Jujubae (FJ) is a classic Chinese herbal medicine formula for treating malignant ascites, which is closely related to the modulation of gut microbiota by our previous study. For water-expelling members (WEM) including EK, EP, and DG may have side effects on the intestine, FJ is employed for detoxification and effectivity enhancement of WEM. However, the underlying mechanism for the compatibility of WEM and FJ is still unknown. Purpose To investigate the effect of the compatibility of WEM with FJ in SZD on malignant ascites and elucidate the potential mechanism from the perspective of the modulation of gut microbiota and related metabolic function. Methods Qualitative and quantitative evaluation of main components was conducted for comprehensive characterization of SZD and WEM. The effect of WEM and SZD was compared on malignant ascites effusion (MAE) rats. The intestinal injury was evaluated by HE staining and oxidative damage. Ascites weight, urine amount, fecal water content, the expression of aquaporins, and cytokines in ascites (IL-6, VEGF, and TNF-α) were measured to estimate the water-expelling activity. The intestinal flora was detected by 16S rDNA sequencing and the content of fecal short-chain fatty acids (SCFAs) was analyzed using gas chromatography-mass spectrometry. Pseudo-germ-free (PGF) and fecal bacteria transplantation animal experiments were subsequently employed to validate this finding. The fecal metabolomics and correlation analysis were finally conducted to explore the related metabolic changes. Results 51 and 33 components were identified in SZD and WEM, respectively. Compared to WEM alone, the compatibility with FJ remarkably reduced intestinal oxidative damage in MAE rats. Ascites was also relieved by downregulating the expression of AQP3 in the colon and decreasing the levels of IL-6, TNF-α and VEGF in ascites. The diversity of gut microbiota was reversed with an increase in Lactobacillus and Clostridia _ UCG-014 while a decrease in Colidextribacter . Under the PGF condition, compatibility of WEM with FJ failed to reduce intestinal injury and alleviate MA significantly, but this effect was further enhanced after FMT. 23 potential fecal metabolites were finally identified. Correlation analysis further showed that Lactobacillus and Clostridia_UCG-014 were positively correlated with SCFAs and l-tryptophan. Colidextribacter was negatively correlated with thymidine but positively correlated with ursodeoxycholic acid and deoxycholic acid. Conclusion FJ cooperated with WEM reduced intestinal injury and alleviated malignant ascites by modulating gut microbiota, short-chain fatty and tryptophan metabolism. These findings provide a scientific basis for the clinical application of FJ from SZD and the safe usage of SZD.
研究领域:
中医
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药理学
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微生物学
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代谢组学
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肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2022
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IF 5.195
Huihui Chen,Yue Zhu,Xiaoping Zhao,Zhenzhong Yang
Ethnopharmacological relevance Tingli Dazao Decoction (TLDZD) recorded in “Synopsis of Prescriptions of the Golden Chamber” is a classical prescription used for the treatment of heart failure nowadays. The studies of TLDZD were mainly focused on clinical practice where the formula was usually combined with other medicinal herbs. Chemical composition and cardiovascular pharmacological research of TLDZD were still insufficient. Aim of the study This study aimed to investigate the chemical constituents of TLDZD, evaluate the effects of TLDZD on mitochondria of myocardial cells under oxidative stress, and identify its potential cardioprotective components. Materials and methods Chemical composition analysis of TLDZD was performed by ultra-performance liquid chromatography-quadrupole-time of flight-mass spectrometry. An in vitro oxidative stress model of cardiomyocytes was established by treating H9c2 cells with tert-butyl hydroperoxide (tBHP). The impact of TLDZD and its components on the production of cellular reactive oxygen species (ROS) and mitochondrial ROS (mROS), the level of malonaldehyde as well as the structure and function of mitochondria were evaluated. The effect of TLDZD on AKT/Nrf2/HO-1 signaling pathway in cardiomyocytes under oxidative stress were observed. Results Seventy-eight compounds were characterized from TLDZD, among which flavonoids , glucosinolates and phenylpropanoids were abundant, and a small number of cardiac glycosides and alkaloids also existed in TLDZD. Pretreatment with TLDZD significantly attenuated cell death , accompanied by decreased ROS and mROS production, reduced malonaldehyde level, lower mitochondrial membrane potential and adenosine triphosphate content in H9c2 cells stimulated with tBHP. The active components were mainly flavonoids of TLZ represented by quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside. In mechanism, the cardioprotective effect of TLDZD was proved to be associated with the activation of the AKT/Nrf2/HO-1 signaling pathway. Conclusions The chemical profile of TLDZD was comprehensively investigated. Flavonoids with quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside as the representative, were the main component in TLDZD responsible for attenuating mitochondrial oxidative damage in cardiomyocytes.
研究领域:
药理学
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心脏病学
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民族药理学
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细胞生物学
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化学成分分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）