SCIENTIA HORTICULTURAE
·
2022
·
IF 4.342
Jiahua Ma,Yahya Faqir,Yunlong Chai,Sanmei Wu,Tong Luo,Shiyu Liao,Abdul Rasheed Kaleri,Chengjia Tan,Yuxin Qing,Mohammad Talib Kalhoro,Niaz Umer,Wazir Hadir
Agriculture lands have low fertilizer retention owing to porosity and irrigation, whereas a significant portion of fertilizer is lost through denitrification, volatilization, surface run-off, and leaching, causing losses of nutrients to air and ground/surface water. Urea fertilizer readily enhances the agricultural yield but also deteriorates the quality of fertile soils. To combat such problems, researchers are seeking to formulate controlled-release or slow-release fertilizers. We used chitosan microspheres as a carrier to encapsulate urea through emulsification and cross-linking techniques to reduce the wastage of urea fertilizer due to evaporation and water flow. Scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy were used to characterize chitosan microspheres and urea-loaded microspheres. Chitosan microspheres and urea-loaded microspheres were authenticated by FT-IR, showing 89% of urea successfully incorporated with chitosan microspheres. Then an experiment was run to compare the effects on plant growth, antioxidant and metabolite contents with and without added nitrogen fertilizer. Various amounts of urea per pot were applied to Chinese cabbage, including T1 0.5433 g, T2 0.6033 g, T3 0.8033 g, CK 0.00, while the same content of urea in chitosan microspheres as T4, T5, T6, and T0 (no urea), respectively. The results indicated that chitosan microspheres-based controlled release nitrogen fertilizers (Cm-CRNFs) had higher seed germination (96%), plant height (17.04 cm), number of leaves (11.00), and leaf length (110.19 mm) as compared to urea and control treatments. In addition, Cm-CRNFs treatment enhanced net photosynthetic rate, stomatal conductance, transpiration rate, fluorescence, dark respiration rate, total sugar, proteins, flavonoids, and polyphenols. Overall, Cm-CRNFs showed promising results in all treatments for all tested physiochemical and growth parameters. It is concluded that controlled release urea fertilizers based on chitosan microspheres can be developed with a simple process. The fertilizers are biocompatible, economically viable, and environment-friendly, and have the potential to prolong the release of macronutrients and micronutrients for plant growth.
研究领域:
农业科学
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植物生理学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Integrative Agriculture
·
2023
·
IF 4.8
Rui-xing ZHANG,Ni-nan ZHANG,Ya-xiu WANG,ABID Khan,Shuai MA,Xue BAI,Qi ZENG,Qi-ming PAN,Bao-hua LI,Lu-gang ZHANG
Carotenoids are involved in the formation of plant leaf color as well as photosystem photoprotection. This study showed that blue light significantly induced up-regulation of the total carotenoid content in the inner leaves of orange-head Chinese cabbage (OHCC). Furthermore, the transcriptomic analysis revealed that blue light treatment induced up-regulation of genes in photosynthesis ( BrHY5-2 , BrCOP1 and BrDET1 ) and the methylerythritol 4-phosphate pathways ( BrGGPS , BrDXS and BrHDR ) upstream of the carotenoid metabolic pathway. Carotenoid metabolomic analysis revealed that the accumulation of several orange and red carotenoids (lycopene, zeaxanthin, β-carotene, lutein, and β-cryptoxanthin) after blue light treatment contributed to the deepening of the leaf coloration, suggesting that short-term blue light treatment could be used to boost nutritional quality. The light signal gene BrHY5-2 participated in the blue light-induced transcriptional regulation of carotenoid biosynthesis in OHCC. Overexpression of BrHY5-2 in Arabidopsis significantly increased the total carotenoid content and the sensitivity to blue light. The above findings revealed new insights about blue-light-induced carotenoid synthesis and accumulation in OHCC lines. They suggested a new engineering approach to increase the nutritional value of vegetables.
研究领域:
植物生理学
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分子生物学
·
营养科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Yahya Faqir,Yunlong Chai,Ali Murad Jakhar,Tong Luo,Shiyu Liao,Mohammad Talib Kalhoro,Chengjia Tan,Sumbal Sajid,Shiqi Hu,Jiali Luo,Shutong Liu,Niaz Umer,Jiahua Ma
Present study investigates the impact of chitosan microspheres-based controlled-release nitrogen fertilizer (Cm-CRNFs) on biological characteristics of Brassica rapa ssp. pekinensis (Chinese cabbage) and soil. The study was carried out under various four treatments, urea (0.8033 g), blank chitosan microspheres (without urea), Cm-CRNFs (0.8033 g), and a control group (CK). The results indicated that Cm-CRNFs significantly prolonged the nitrogen release and enhanced the plant shoot length, shoot diameter, number of branches, pods, total amino acids, and vitamin C of Brassica rapa ssp. pekinensis as well as increased the soil nutrient availability. Chao index of bacterial diversity analysis showed a significant reduction of 15.89 % in Cm-CRNFs, but the Shannon index value in Cm-CRNFs was increased by 23.55 % compared to CK. Furthermore, Cm-CRNFs treatment significantly influenced genus richness level of Arthrobacter , Archangium , Bacillus , and Flavihumibacter . Moreover, relative abundance of bacteria significantly enhanced Cm-CRNFs, including Acidobacteriota , Acitinobacteriota , Cloroflexi , Cyanobacteria , and Patescibacteria . Soil enzyme activity such as: urease, acid phosphatase, and catalase enzymes in Cm-CRNFs and urea treatment significantly increased. Besides, other enzymes such as: cellulase and β-glucosidase activity decreased in the Cm-CRNFs treatment. It was concluded that Cm-CRNFs potentially prolonged discharge of micro/macronutrients and improved soil bacterial diversity, which ultimately enhanced the soil fertility and improved the soil enzyme activity.
研究领域:
农业
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环境科学
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微生物学
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植物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plants-Basel
·
2025
·Vol. 14
·期 14
·2195
·
IF 4.1
Shan Chen,Yang Zheng,Qing Wang,Rong Mu,Xianchao Sun,Guanhua Ma,Liezhao Liu,Jiequn Ren,Kuo Huang,Guokang Chen
Clubroot, caused byPlasmodiophora brassicaeWoronin, poses a major threat to Chinese cabbage (Brassica rapasubsp.pekinensis) production worldwide, significantly impacting crop yield, quality, and economic value. Biological control represents a promising approach since it is non-toxic and eco-friendly, and it reduces the risk of pathogen resistance development. In this study, our objective was to screen for actinomycetes that can effectively inhibit clubroot. We screened 13 actinomycete strains, identifying 2, XDS3-6 and CD1-1, with substantial in vivo inhibitory effects, achieving infection suppression rates above 64% againstP. brassicae. Phylogenetic analysis classified XDS3-6 and CD1-1 asStreptomyces virginiaeandStreptomyces cinnamonensis, respectively. Both strains exhibited protease and glucanase production capabilities, essential for pathogenic suppression. Additionally, these strains induced host defense responses, as evidenced by increased jasmonic acid (JA) and salicylic acid (SA) accumulation and elevated activities of defense-related enzymes. Colonization studies of XDS3-6 and CD1-1 mutant strains in cabbage roots indicated sustained root colonization, with peak colony-forming units (CFUs) at 20 days post-inoculation, reaching 11.0 × 104CFU/g and 8.5 × 104CFU/g, respectively, and persisting for at least 30 days. Overall, these findings underscore the potential ofStreptomycesstrains XDS3-6 and CD1-1 as effective biocontrol agents, providing a theoretical foundation for their application in managing clubroot in Chinese cabbage.
研究领域:
植物病理学
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微生物学
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农学
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生物防治
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植物病害学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Ting Lv,Boxu Wang,Ningyi Xu,Baitong Shang,Ni Liu,Changshun Su,Chuanyu Yang,Hongxia Li,Zhenlin Xu,Chunyan Sun
Understanding the residues and degradation of organophosphorus pesticides (OPs) in crops has attracted increasing attention. Herein, we designed a sensitive fluorescence immunoassay (FIA) by employing nanobody-linked alkaline phosphatase (Nb-ALP) and gold nanoclusters anchored manganese dioxide (AuNCs-MnO 2 ) composite. In immunoassay protocol, Nb-ALP is used to competitively recognize the coating antigen and pesticide. After competitive immunoreaction, alkaline phosphatase catalyzes l -ascorbic acid-2-phosphate to produce ascorbic acid that can trigger the decomposition of the AuNCs-MnO 2 composite, regulating the fluorescence response. As a proof-of-concept, fenitrothion (FNT) is chosen as the target analyte. As a result, the developed FIA exhibits high detection sensitivity (IC 10 = 5.78 pg/mL), which is about 56-times higher than that of the conventional enzyme-linked immunosorbent assay. The developed FIA has been successfully applied for precisely monitoring the degradation of FNT in Chinese cabbage with excellent anti-interference ability and reproducibility, paving the way for the determination of pesticide residues in real food samples.
研究领域:
分析化学
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环境科学
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农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Yu-Qi Zhang,Zhen-Lin Xu,Feng Wang,Jun Cai,Jie-Xian Dong,Jin-Ru Zhang,Rui Si,Cheng-Long Wang,Yu Wang,Yu-Dong Shen,Yuanming Sun,Hong Wang
A heavy chain variable fragment of heavy chain only antibodies derived from camelids termed VHH shows beneficial characteristics for immunoassay in terms of high sensitivity, outstanding stability and ease in expression. In the present study, we isolated six VHHs from phage display library against parathion, which is a widely used organophosphorus pesticide with high toxicity and persistence. One of six selected VHHs named VHH9, showed highest specificity and superior thermo-stability. A VHH9-alkaline phosphatase (AP) fusion was constructed and used to establish a one-step direct competitive fluorescence enzyme immunoassay (dc-FEIA) with a half maximal inhibitory concentration (IC50) of 1.6 ng/mL and a limit of detection of 0.2 ng/mL which was 4-fold or 3-fold higher sensitivity than direct competitive enzyme-linked immunoassay (dc-ELISA) and indirect competitive enzyme-linked immunoassay (ic-ELISA) for parathion. Furthermore, our assay indicated a 50% reduction on operation time compared with the ic-ELISA method. The presented immunoassay was validated with spiked Chinese cabbage, cucumber, and lettuce samples, and confirmed by UPLC-MS/MS. The results indicated that the VHH-AP-based dc-FEIA is a reproducible detection assay for parathion residues in vegetable samples.
研究领域:
生物化学
·
分析化学
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环境科学
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农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Le Sheng,Yulong Jin,Yonghuan He,Yanyan Huang,Liushui Yan,Rui Zhao
Superparamagnetic core-shell molecularly imprinted polymer nanoparticles (MIPs) were prepared via surface initiated reversible-addition fragmentation chain transfer (si-RAFT) polymerization for the selective recognition of 2,4-dichlorophenoxyacetic acid (2,4-D) in real samples. The construction of uniform core-shell structure with a 50 nm MIP layer was successfully accomplished, which favored mass transfer and resulted in fast recognition kinetics. The static equilibrium experiments revealed the satisfied adsorption capacity and imprinting efficiency of Fe 3 O 4 @MIP. Moreover, the Fe 3 O 4 @MIP exhibited high selectivity and affinity towards 2,4-D over structural analogues. The prepared Fe 3 O 4 @MIP nanoparticles were used for the selective enrichment of 2,4-D in tap water and Chinese cabbage samples. Combined with RP-HPLC, the recoveries of 2,4-D were calculated from 93.1% to 103.3% with RSD of 1.7–5.4% (n = 3) in Chinese cabbage samples. This work provides a versatile approach for fabricating well-constructed core-shell MIP nanoparticles for rapid enrichment and highly selective separation of target molecules in real samples.
研究领域:
分析化学
·
环境科学
·
材料科学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Ruixing Zhang,Wenjing Yang,Qiming Pan,Qi Zeng,Chengtai Yan,Xue Bai,Yao Liu,Lugang Zhang,Baohua Li
The aim of this study was to investigate the impact of long-term exposure to blue light-emitting diodes (LEDs) on the accumulation of indolic glucosinolates and carotenoids, as well as the plant growth and antioxidant activities in both orange and common Chinese cabbage ( Brassica rapa L. ssp. pekinensis ). Blue light treatment also induced higher ferric-reducing antioxidant power and 2,2-diphenyl-1-picrylhydrazyl by 20.66 % and 30.82 % and antioxidant enzyme activities catalase, peroxidase, superoxide dismutase, and the accumulation of non-enzymatic antioxidant substances (total phenols and total flavonoids) in the orange Chinese cabbage. Furthermore, long-term exposure to blue light had negative effects on the net photosynthetic rate and chlorophyll fluorescence levels. Meanwhile, blue light promoted accumulation of Indol-3-ylmethyl glucosinolate (I3M), β-carotene, lutein and zeaxanthin due to the high expression of regulatory and biosynthetic genes of the above metabolic pathways. In particular, lycopene and β-carotene content in orange Chinese cabbage increased by 60.14 % and 65.33 % compared to the ones in common line. The accumulation of carotenoid and increasing antioxidant levels in the orange cabbage line was influenced by long-term blue light irradiation, leading to better tolerance to low temperature and drought stresses. The up-regulation of transcription factors such as BrHY5-2 , BrPIF4 and BrMYB12 may also contribute to the increased tolerance in orange Chinese cabbage to extreme environmental stresses. The BrHY5-2 gene could activate carotenoid biosynthetic genes and induce the accumulation of carotenoids. These findings suggested that long-term blue light irradiation could be a promising technique for increasing the nutrition value and enhancing tolerance to low temperature and drought stresses in Chinese cabbage.
研究领域:
植物科学
·
农业生物技术
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分子生物学
·
植物生理学
·
抗逆性
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）