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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY · 2014 ·Vol. 49 ·期 10 ·2290-2298 · IF 1.354

Characterisation and comparison of phenols, flavonoids and isoflavones of soymilk and their correlations with antioxidant activity

Yurong Ma,Huihua Huang

Summary To investigate the relationship between bioactive compounds and antioxidant property, total phenolic content (TPC), total flavonoid content (TFC), individual and total isoflavone content of soymilk and their correlations with oxygen-radical-absorbing capacity (ORAC), ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity values were evaluated and compared. Results showed that TPC, TFC, isoflavones (including concentration and profiles) and antioxidation activity significantly varied among ten tested soybean cultivars. Significant correlations were established between DPPH and TFC ( r = 0.553, P < 0.01), DPPH and TPC ( r = 0.753, P < 0.01), FRAP and TFC ( r = 0.599, P < 0.01) and FRAP and TPC ( r = 0.616, P < 0.01). Positive linear correlations were found between subtotal, total isoflavones and ORAC. Unlike other isoflavone monomers, aglycone isoflavones correlated positively with DPPH and FRAP significantly. DPPH well correlated with FRAP, whereas neither DPPH nor FRAP correlated with ORAC. TPC, TFC, individual and total isoflavone values are potentially useful for soymilk antioxidant activity assessment.

研究领域: 食品科学 · 营养学
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ULTRASONICS SONOCHEMISTRY · 2022 · IF 9.336

Fabrication of icariin-soymilk nanoparticles with ultrasound-assisted treatment

Jinping Wang,Hong Zhu,Yueming Jiang,Jianbo Xiao,Bao Yang,Lingrong Wen

Ultrasound is effective to fabricate nanocomplex. Soymilk is a natural nanocarrier with good compatibility. However, information about soymilk-nutraceuticals nanocomplex is limited. In this work, soymilk was used to encapsulate icariin, a well known nutraceutical with poor bioavailability. The effect of ultrasound on the quality of icariin-soymilk nanocomplexes (ISNCs) was investigated. Ultrasound could reduce the particle size, improve the surface hydrophobicity and change the microstructure of soymilk. With increasing ultrasound treatment time, an increased surface hydrophobicity was observed. The highest encapsulation efficiency (89.67 %) and loading capacity (28.92 µg/mg) were found for USI-20, whereas the smallest particle size (132.47 nm) was observed for USI-120. USI-60 showed the lowest ζ-potential (-31.33 mV) and the highest bioaccessibility (76.08 %). Ultrasound could enhance the storage stability of ISNCs. The data of NMR and fluorescence indicated that ISNCs were mainly stabilized by hydrophobic interaction.

CAS / MDL: 7647-14-5 489-32-7
研究领域: 食品科学 · 药剂学
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Food Science and Human Wellness · 2023 · IF 7

Structure, bioavailability and physicochemical properties of icariin-soymilk nanoparticle

Jinping Wang,Lingrong Wen,Yueming Jiang,Hong Zhu,Weizheng Sun,Guangyi Dai,Bao Yang

Soymilk is a natural nanocarrier. However, the performance of flavonoid-soymilk nano-complex remains unclear. In this work, icariin-soymilk nano-complexes (ISNCs) were successfully fabricated and characterized. The effects of high-pressure homogenization (HPH) treatment on structure and physicochemical properties of soymilk and nano-complexes were investigated. HPH treatment could significantly improve the surface hydrophobicity and interfacial activity of soymilk. The soymilk with HPH treatment could significantly improve the water solubility (20 folds), thermal stability and bioavailability of icariin. The highest encapsulation efficiency (93.28 %), loading capacity (39.09 µg/mg), ζ-potentia (absolute value, 31.20 mV) and bioavailability (72.14 %) were observed in HSI-200 (200 bar of homogenization pressure). While HSI-500 (500 bar of homogenization pressure) showed the smallest particle size (183.73 nm). ISNCs showed a rougher surface and an irregular lamellar structure with large amount of fine particles by using Cryo-SEM, suggesting that icariin was encapsulated in soymilk. These data supplied a novel strategy to improve the performance of icariin in functional foods.

CAS / MDL: 489-32-7
研究领域: 食品科学 · 药理学
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FOOD CHEMISTRY · 2023 · IF 8.8

Nutritional and functional insight into novel probiotic lycopene-soy milk by genome edited Bacillus subtilis

Hao Luo,Yihong Bao,Ping Zhu

Nutritional and functional soy-based milk gains growing attention globally in food industry. However, its poor sensorial attributes, single flavor, and limited substance variety become critical issues in displaying balanced nutrition and multifunction for health. Herein, a novel probiotic lycopene-soy milk was developed by genome edited Bacillus subtilis harboring lycopene biosynthesis cassette with efficient lycopene production of 25.73 ± 1.57 mg/g DCW. Further investigation displayed desirable pH, reducing sugar, protein, total phenolic content and isoflavone for achieved milk than conventional soy milk , implying it with well-balanced nutritional quality . Notably, achieved milk exhibited stronger antioxidant capacity and higher isoflavone bioavailability for functionality. Moreover, it possessed significantly high scores for taste, appearance, and overall acceptability, suggesting its excellent sensorial attributes. To our delight, it is the first time to fortify soy-milk with probiotic and lycopene by genome edited B. subtilis to explore additive effect on improving nutritional value and functionality for food application.

研究领域: 食品科学 · 微生物学 · 营养学
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ChemistryOpen · 2020 ·Vol. 9 ·期 5 ·582-587 · IF 2.37

Dual-Templating Approaches to Soybeans Milk-Derived Hierarchically Porous Heteroatom-Doped Carbon Materials for Lithium-Ion Batteries

Peng Yan,Huaibo Ye,Yang Han,Jingjing Wang,Fenfen Zheng,Weiwei Xiong,Hongxun Yang,Junhao Zhang,Aihua Yuan,Xingcai Wu

Graphical Powerful soybean ! This work provides novel strategies to design nanostructures by using low-cost carbonaceous materials, thus facilitating their applications as energy storage materials. Hierarchically porous heteroatom-doped carbon materials have been prepared using soybean milk precursors. The as-derived hierarchically porous nitrogen/oxygen-doped carbon materials show excellent electrochemical properties for LIBs and SIBs. Biomass derived carbon materials are widely available, cheap and abundant resources. The application of these materials as electrodes for rechargeable batteries shows great promise. To further explore their applications in energy storage fields, the structural design of these materials has been investigated. Hierarchical porous heteroatom-doped carbon materials (HPHCs) with open three-dimensional (3D) nanostructure have been considered as highly efficient energy storage materials. In this work, biomass soybean milk is chosen as the precursor to construct N, O co-doped interconnected 3D porous carbon framework via two approaches by using soluble salts (NaCl/Na 2 CO 3 and ZnCl 2 /Mg 5 (OH) 2 (CO 3 ) 4 , respectively) as hard templates. The electrochemical results reveal that these structures were able to provide a stable cycling performance (710 mAh ⋅ g −1 at 0.1 A ⋅ g −1 after 300 cycles for HPHC-a, and 610 mAh ⋅ g −1 at 0.1 A ⋅ g −1 after 200 cycles for HPHC-b) in Li-ion battery and Na-ion storage (210 mAh ⋅ g −1 at 0.1 A ⋅ g −1 after 900 cycles for HPHC-a) as anodes materials, respectively. Further comparative studies showed that these improvements in HPHC-a performance were mainly due to the honeycomb-like structure containing graphene-like nanosheets and high nitrogen content in the porous structures. This work provides new approaches for the preparation of hierarchically structured heteroatom-doped carbon materials by pyrolysis of other biomass precursors and promotes the applications of carbon materials in energy storage fields.

研究领域: 材料科学 · 能源存储
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Analytical Methods · 2018 ·Vol. 11 ·期 3 ·317-326 · IF 2.073

A new boronate-affinity hollow solid phase extraction adsorbent for the enrichment of cis-diol-containing isoflavones in soybean milk samples

Xingyu Hou,Wei Huang,Fugui Zhu,Fang Geng,Miaomiao Tian

In this study, a new boronate-affinity hollow adsorbent was successfully fabricated. This adsorbent was prepared using mesoporous MCM-48 as a sacrificial support, methacrylic acid (MAA), N,N-methylenebisacrylamide (MBA) and azobisisobutyronitrile (AIBN) were applied as a functional monomer, cross-linker and initiator, respectively, and further modified with boronic acid groups (4-vinylphenylboronic acid, VPBA) to improve the enrichment efficiency. Such materials were composed of a hollow structure, a hydrophilic polymer coating of poly(MBA-MAA), and a VPBA-functionalized shell designed for capturing isoflavones. The resulting material coupled with high-performance liquid chromatography (HPLC) was applied to analyze three cis-diol isoflavones (daidzin, glycitin, and genistin) in soybean milk samples. The sorbent was characterized by transmission electron microscopy, Fourier transform infrared spectrometry and thermo-gravimetric analysis. Several parameters affecting the extraction efficiency were optimized. Owing to the hollow structure, more binding sites were located on the inner and outer surfaces of this material, which could facilitate the binding of the target molecules to the adsorbent. Adsorption selectivity studies indicated that the prepared material can selectively recognize isoflavones. Under optimized conditions, the limits of detection of the method were 3.8 to 7.3 μg L−1 for the three isoflavones based on a signal-to-noise ratio of 3 : 1. The spiked recoveries were found to be between 67.4 and 104.7% (the relative standard deviations were less than 3.8%, n = 3). This indicated the superiority of the method in selective extraction of isoflavones even from complex matrices.

研究领域: 分析化学 · 食品科学
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Analytical Methods · 2014 ·Vol. 6 ·期 18 ·7361-7366 · IF 1.938

Determination of phthalate acid esters in soybean milk using dispersive liquid–liquid microextraction coupled with gas chromatography and mass spectrometric detection

Songchun Bai,Xinxiu Wang,Fan Hu,Tianlin Wang,Ping Cheng,Zhen Zhou

Dispersive liquid–liquid microextraction (DLLME) was coupled with gas chromatography and mass spectrometric detection for the determination of eight phthalate acid esters (PAEs) in soybean milk. Parameters impacting the extraction efficiencies were optimized including organic solvents to extract PAEs from soybean milk, salt concentrations and organic solvents for DLLME. Under the optimal conditions, limits of detection (LODs) and limits of quantification (LOQs) were in the range of 0.57–0.79 and 1.90–2.63 ng g−1, respectively. Linearities varied in the range of 1–16 000 ng g−1 with the correlation coefficients of 0.9993–0.9998. The precision of the method was 2.9–3.2 in terms of RSD% based on triplicate measurements. The preconcentration factors were in the range of 200–260. The recoveries of eight PAEs were in the range from 79.0 to 110% at three spiked levels. The trace PAEs in five different brands of soybean milk purchased from the market were determined successfully.

研究领域: 分析化学 · 食品安全
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CARBOHYDRATE POLYMERS · 2017 · IF 4.811

Rhein functionalized magnetic chitosan as a selective solid phase extraction for determination isoflavones in soymilk

Hui Guo,Lu Xue,Shen Yao,Xiaohua Cai,Junqing Qian

A new solid-phase extraction mode was developed for separation some flavones from the complex interference system. In the experiment, rhein anchored on magnetic chitosan microparticles, prepared facilely without removal of the template molecule, was used as sorbents for the extraction of target analytes; after completion of the extraction process, Fe 3 O 4 particles acted as carrier to retrieve rhein functionalized magnetic chitosan microparticles (RMCMPs) from the sample solution. RMCMPs showed excellent selective adsorption capacity of isoflavones. The result suggested that the method was useful for fast determination and quantification of isoflavones in soymilk. The RMCMPs were characterized by scanning electron microscope (SEM), X-ray diffraction (XRD) and Fourier transforms infrared spectrometer (FTIR).

CAS / MDL: 919-30-2 4432-31-9
研究领域: 分析化学 · 食品科学 · 材料科学
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JOURNAL OF FOOD SCIENCE · 2025 ·Vol. 90 ·期 9 ·e70509 · IF 3.4

Ovotransferrin Fibrils Formed by Peptides Self-Assembly Improve the Stability and Antioxidation of Quercetin and Their Application in Quercetin Fortified Soybean Milk

Jipeng Zhang,Yifan Li,Tong Wu,Haoran Liu

Quercetin (Que) is a natural flavonoid compound with multiple biological functions. However, it is easily dissociated by external conditions and difficult to apply in the food industry, so it needs to be encapsulated with food-derived nanocarriers to improve its stability. Here, ovotransferrin (OVT) is hydrolyzed into peptides by subtilisin A, and then the peptides self-assemble into hollow nanofibrils under Ca 2+ ion induction at 50°C and pH 7.5 for 1 h. The increase in Thioflavin T (ThT) fluorescence intensity confirms the continuous growth of OVT fibrils by β-sheet stacking. The mature OVT fibrils have a length of 1–2 µm and a diameter of 10 nm. In addition, the increase in G′ reflects the enhanced stiffness or deformation resistance of OVT fibrils. As nanocarriers, OVT fibrils successfully encapsulate Que with a maximum loading rate of (7.41 ± 0.36)% by hydrophobic interaction. OVT fibrils improve the chemical stability and antioxidant property of Que. Further, we developed a novel Que-fortified soybean milk drink by adding Que-loaded OVT fibrils into soybean milk in a volume ratio of 1:5. OVT fibrils improve the stability and viscosity of Que-fortified soybean milk drink. By sensory evaluation, OVT fibrils can play an important role in masking the odor of Que and protecting its color. This work protects Que from degradation through nanoencapsulation technology, while endowing soybean milk with antioxidant, anti-inflammatory, and other effects, providing a new strategy for the development of protein drinks with nutritional enhancement and health effects. Practical Applications This study used food grade nanomaterials encapsulation technology to improve the stability and antioxidant properties of Que, and added them to soybean milk to enhance the nutritional and health benefits of soybean milk.

CAS / MDL: 1898-66-4
研究领域: 食品科学 · 纳米技术 · 生物材料 · 分子生物学 · 营养学 · 胶体与界面科学
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FOOD CHEMISTRY · 2020 · IF 6.306

Effect of soaking conditions on the formation of lipid derived free radicals in soymilk

Xiaoxiao Feng,Yufei Hua,Caimeng Zhang,Xiangzhen Kong,Xingfei Li,Yeming Chen

Lipid derived free radical in soymilks were studied by combining 5,5-dimethyl-pyrroline-l-oxide (DMPO) spin trap, chloroform-methanol extraction and electron spin resonance (ESR) spectroscopy. Five lipid derived free radical adducts: DMPO-X , DMPO-L , DMPO-R , DMPO-LOO , and DMPO-RO were presented in soymilks. The total amounts of spins increased as the soaking temperature increased from 4 °C to 50 °C and the soaking pH increased from 3 to 9 and in paralleled with the diffusion of soybean exudates to soaking water. Prolonged soaking of soybean at 50 °C resulted in a higher signal intensity of DMPO-R than that of DMPO-LOO . Soybean lipoxygenases (LOXs) were responsible for the formation of lipid derived free radicals in soymilks. Soybean exudates affected the total amounts of lipid radicals in linoleic acid (LA) – LOX model system. The relative signal intensities of DMPO-R and DMPO-LOO were depended on the contents of soybean exudates in the system.

CAS / MDL: 60-33-3
研究领域: 食品科学 · 生物化学
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