Preparation of curcumin@Blumea balsamifera oil-Bletilla striata polysaccharide nanostructured lipid carrier based on “Medicines and Excipients all-in-one” and its scarless wound healing effect
Skin scarring after injury represents a global challenge, imposing significant impacts on patients' appearance, function, and mental health, thus highlighting the clinical urgency of exploring scar prevention strategies. This study developed a multifunctional nanostructured lipid carrier hydrogel for promoting scarless wound healing based on the innovative concept of “Medicines and Excipients all-in-one”. Using anti-inflammatory and wound-healing Bletilla striata polysaccharide (BSP) and Blumea balsamifera oil (BBO) as functional excipients, we prepared integrated BBO-BSP nanostructured lipid carriers (NLC). Here, BSP served as a natural polymeric emulsifier, while BBO served as the active liquid oil. Furthermore, with BSP as the gel matrix, BBO-BSP NLC hydrogel (NLCG) were constructed, and then curcumin was loaded to form Cur@BBO-BSP NLCG, achieving the synergistic wound-healing function of multiple components. Characterization showed that Cur@BBO-BSP NLC had an average particle size of 172.6 ± 1.0 nm, a polydispersity index of 0.106 ± 0.037, an entrapment efficiency of 89.52 ± 2.28 %, and a drug loading capacity of 4.73 ± 0.08 %. Cur@BBO-BSP NLCG significantly enhanced curcumin stability, exhibiting sustained release over 264 h in vitro . Antioxidant assays revealed that Cur@BBO-BSP NLCG scavenged DPPH and hydroxyl radicals at rates of 94.10 ± 3.24 % and 94.88 ± 4.03 %, respectively, while demonstrating good biosafety and promoting L929 cell migration. In vivo experiments in rabbit ear models confirmed that Cur@BBO-BSP NLCG accelerated wound healing and inhibited scar formation. The mechanism may be related to its ability to regulate the expression of factors such as CD31, VEGF, TNF-α, IL-6, and TGF-β 1 , thereby enabling multidimensional regulation of vascular remodeling, inflammatory microenvironment and fibroblast function. The proposed “functional excipient-active medicine” collaborative delivery strategy offers a promising new paradigm for scarless wound healing therapy.
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