ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Zhihao Xu,Man Liu,Qiyi He,Wenkai Li,Junkang Pan,El-Sayed A. El-Sheikh,Wenzhi Liu,Bruce D. Hammock,Dongyang Li
Aquatic viruses cause devastating diseases in aquaculture, severely limiting production and resulting in significant economic losses. The giant salamander iridovirus (GSIV), a member of the genus Ranavirus , is the only virus reported to infect giant salamanders, posing a severe threat to the farming industry. Currently, there are no effective strategies available for its control. In this context, a reliable diagnostic tool for the rapid detection of GSIV is crucial to mitigate its impact. This study developed a nanobody-based immunoassay for the rapid and reliable detection of GSIV. GSIV was cultured and used to immunize an alpaca, A phage library with an original diversity of 1.89 × 10 8 PFU was established, and six nanobodies were identified after three rounds of panning. Among them, HC-2 exhibited superior performance and was used to develop a highly sensitive ELISA method employing streptavidin-PolyHRP (SA-PolyHRP) as a signal amplification strategy. The assay achieved a detection limit of 3.3 × 10 5 PFU/mL and demonstrated high specificity without cross-reactivity. Practical application was validated in infected giant salamander samples, underscoring its diagnostic potential. This work provides a robust tool for GSIV diagnosis and showcases the potential of nanobodies in advancing aquaculture diagnostics.
研究领域:
水产养殖学
·
病毒学
·
诊断学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2024
·
IF 8.1
Ying Liu,Yaxi Wang,Hongyuan Wang
Ciprofloxacin (CIP) and levofloxacin (LEV) are broad-spectrum antibiotics with potent antibacterial activity . Although many studies have shown that antibiotics can lead to gut microbiota disruption, the effects of CIP and LEV on gut microbial colonization at the embryonic stage remain poorly characterized. Here, we evaluated the response of Bufo gargarizans embryos in terms of gut microbiota colonization, growth and developmental stages to CIP and LEV exposure. Embryos treated with 100 μg/L CIP and LEV exhibited significantly reduced diversity and richness of the gut microbiota, as well as altered community structure. Both CIP and LEV treatments resulted in an increase in the pathogenic bacteria Bosea and Aeromonas , and they appeared to be more resistant to CIP than LEV. Additionally, CIP exposure caused reduced total length and delayed the development in B. gargarizans embryos, while LEV increased the total length and promoted embryonic development. The present study revealed the adverse effects of CIP and LEV exposure on host gut microbiota, growth and development during the embryonic stage, and contributed new perspectives to the evaluation of early aquatic ecological risk under CIP and LEV exposure.
研究领域:
生态学
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微生物学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·
IF 3.3
Fan-Yu He,Xing-Tong Zhu,Hui Liu,Yun-Q ing Chong,Zhi-Ping Wu,Lu-Jun Ye,Yue-Wen Chen,Jing-Jing Fu
BACKGROUND Chinese giant salamander protein hydrolysates (CGSPH) are beneficial to human health as a result of their high content of amino acids and peptides. However, the formation of bitter peptides in protein hydrolysates (PHs) would hinder their application in food industry. The ultrasound assisted wet-heating Maillard reaction (MR) is an effective way to improve the flavor of PHs. Thus, the effect of ultrasonic assisted wet-heating MR on the structure and flavor of CGSPH was investigated in the present study. RESULTS The results indicated that the ultrasound assisted wet-heating MR products (MRPs) exhibited a higher degree of graft and more significant changes in the secondary and tertiary structures of CGSPH compared to traditional wet-heating MRPs. Moreover, ultrasound assisted wet-heating MR could significantly increase the content of small molecule peptides and reduce the content of free amino acids of CGSPH, which resulted in more significant changes in flavor characteristics. The changed in flavor properties after MR (especially ultrasound assisted wet-heating MRPs) were mainly manifested by a significant reduction in bitterness, as well as a significant increase in the content of aromatic aldehyde ester compounds such as furan-2-carbaldehyde, butanal, benzaldehyde, furfural, etc. CONCLUSIONS Ultrasound assisted wet-heating MR between CGSPH and xylose could be a promising way to improve the sensory characteristics of CGSPH. © 2024 Society of Chemical Industry.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
Food Science and Human Wellness
·
2024
·
IF 5.6
Wei Li,Haihong Chen,Hongliang Chen,Ziyin Li,Wei Hu,Qinxuan Zhou,Bing Xu,Yi Wang,XinHui Xing
Hyperuricemia (HUA) is a vital risk factor for chronic kidney diseases (CKD) and development of functional foods capable of protecting CKD is of importance. This paper aimed to explore the amelioration effects and mechanism of Andrias davidianus bone peptides (ADBP) on HUA-induced kidney damage. In the present study, we generated the standard ADBP which contained high hydrophobic amino acid and low molecular peptide contents. In vitro results found that ADBP protected uric acid (UA)-induced HK-2 cells from damage by modulating urate transporters and antioxidant defense. In vivo results indicated that ADBP effectively ameliorated renal injury in HUA-induced CKD mice, evidenced by a remarkable decrease in serum UA, creatinine and blood urea nitrogen, improving kidney UA excretion, antioxidant defense and histological kidney deterioration. Metabolomic analysis highlighted 14 metabolites that could be selected as potential biomarkers and attributed to the amelioration effects of ADBP on CKD mice kidney dysfunction. Intriguingly, ADBP restored the gut microbiome homeostasis in CKD mice, especially with respect to the elevated helpful microbial abundance, and the decreased harmful bacterial abundance. This study demonstrated that ADBP displayed great nephroprotective effects, and has great promise as a food or functional food ingredient for the prevention and treatment of HUA-induced CKD.
研究领域:
营养学
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药理学
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肾脏生理学
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代谢组学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
Food Biophysics
·
2024
·Vol. 19
·期 2
·310-320
·
IF 2.8
Wu Zi-ke,Chen Ru-kang,Shi Yu,Zhu Chen-min,Wang Yi-ran,Shi Yu-gang,Ettelaie Rammile,Gu Qing
Octyl gallate (GAC8) as a bioactive compound has excellent antibacterial effectiveness, but its poor hydrophilicity limits its applications. In this work, GAC8 was encapsulated into hydroxypropyl- β -cyclodextrin (HP β CyD) cavity to form an inclusion complex (GAC8/HP β CyD-IC), and their antibacterial activities were investigated. Phase solubility test suggested that the aqueous solubility of GAC8 was prominently enhanced after forming the inclusion complex. The aqueous solution of GAC8/HP β CyD-IC yielded uniform fiber morphology with ~ 900 nm average fiber diameter. The fabricated GAC8/HP β CyD-IC nanofibers (GAC8/HP β CyD-IC NFs) were characterized by 1 H NMR, FT-IR, XRD, DSC, and TGA, revealing successful synthesis of GAC8/HP β CyD-IC NFs and the thermal stability of GAC8 was enhanced by inclusion complexation with HP β CyD. Furthermore, GAC8/HP β CyD-IC NFs possessed antibacterial activity against E. coli (12.5 mm zone of inhibition), S. aureus (18.5 mm zone of inhibition). The results of DNA and protein leakage in the experiment indicated that GAC8/HP β CyD-IC NFs can disrupt the membrane integrity of bacteria. Meanwhile, GAC8/HP β CyD-IC NFs suppressed the colony growth of E. coli on Chinese giant salamander meat. Overall, the nanofibers encapsulating GAC8/HP β CyD-IC were potential antibacterial food packaging materials.
研究领域:
食品包装
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抗菌材料
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纳米技术
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食品保鲜
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高分子科学
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Yu-gang Shi,Chen-min Zhu,Dong-hui Li,Ze-yu Shi,Qing Gu,Yue-wen Chen,Jie-qian Wang,Rammile Ettelaie,Jian-She Chen
An excellent bactericidal effect of octyl gallate (OG)-mediated photodynamic inactivation (PDI) against foodborne pathogens (Escherichia coli and Staphylococcus aureus) was evaluated in relation to the mode of action. UV-A irradiation (wavelength, 365 nm; irradiance, 8.254 ± 0.18 mW/cm2) of the bacterial suspension containing 0.15 mM OG could lead to a >5-log reduction of viable cell counts within 30 min for E. coli and only 5 min for S. aureus. Reactive oxygen species (ROS) formation was considered the main reason for the bactericidal effect of OG + UV-A light treatment because toxic ROS induced by OG-mediated PDI could attack the cellular wall, proteins, and DNA of microbes. Moreover, the bactericidal effect, as well as the yields of ROS, depended on OG concentrations, irradiation time, and laser output power. Furthermore, we prepared an edible photodynamic antimicrobial membrane comprising electrospun cyclodextrin nanofibers (NFs) by embedding OG. The resultant OG/HPβCD NFs (273.6 μg/mL) under UV-A irradiation for 30 min (14.58 J/cm) could cause a great reduction (>5-log) of viable bacterial counts of E. coli. The in situ photodynamic antibacterial activity of OG/HPβCD NF-based packaging was evaluated during the Chinese giant salamander storage. Overall, this research highlights the dual functionalities (antibacterial and photodynamic properties) of OG as both an antibacterial agent and photosensitizer and the effectiveness of electrospun NFs containing OG as an active antibacterial packaging material for food preservation upon UV light illumination.
研究领域:
食品科学
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微生物学
·
生物技术
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2020
·Vol. 137
·期 46
·49521
·
IF 2.52
Xuan Ji,Guomin Liu,Yutao Cui,Wenyuan Jia,Yungang Luo,Zhiqiang Cheng
In this article, a hybrid system of hydrogel/frog egg-like microspheres (H-FMS) formed by the combination of coaxial electrostatic spraying and freeze-drying was introduced for enhancing wound healing efficiency through the sustained release of Rana chensinensis skin peptides (RCSPs). The porous PVA/gelatin hydrogel were obtained and frog egg-like microspheres (FMS) of sodium alginate (SA), shaping uniform and smooth, were embedded into hydrogel. Based on PVA/gelatin hydrogel, the FMS addition increased the water absorption of hydrogel to 1,105%. RCSPs were more effectively encapsulated into FMS than solid microspheres (MS). Not only does the H-FMS act as good “depots” for sustained release of RCSPs over 9 days, without exhibiting obvious burst release, but also show good biocompatibility in vitro. In vivo studies on wound healing as well as the histology of fibroblasts, re-epithelialization, inflammation, and hair follicles indicated that the structure of H-FMS released RCSPs continuously and promoted wound healing in rats significantly.
研究领域:
生物材料
·
药剂学
·
伤口愈合
·
组织工程
✓ 已验证
产品关联
DOI
高影响因子
iScience
·
2022
·Vol. 25
·期 10
·105106-105106
·
IF 6.107
Yuqing Liu,Yinghao Li,Haitao Shang,Wen Zhong,Quan Wang,Kibret Mequanint,Chuhong Zhu,Malcolm Xing,Hong Wei
The widespread use of biological tissue adhesives for tissue repair is limited by their weak adhesion in a wet environment. Herein, we report the wet adhesion mechanism of a dry granular natural bioadhesive from Andrias davidianus skin secretion (ADS). Once contacting water, ADS granules self-assemble to form a hydrophobic hydrogel strongly bonding to wet substrates in seconds. ADS showed higher shear adhesion than current commercial tissue adhesives and an impressive 72-h underwater adhesion strength of ∼47kPa on porcine skin tissue. The assembled hydrogel in water maintained a dissipation energy of ∼8 kJ/m 3 , comparable to the work density of muscle, exhibiting its robustness. Unlike catechol adhesion mechanism, ADS wet adhesion mechanism is attributed to water absorption by granules, and the unique equilibrium of protein hydrophobicity, hydrogen bonding, and ionic complexation. The in vivo adhesion study demonstrated its excellent wet adhesion and hemostasis performance in a rat hepatic and cardiac hemorrhage model.
研究领域:
生物材料
·
组织工程
·
生物医学工程
·
止血
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Jing Song,Fengyun Huang,Kun Ma,Rui Ding,Kai Tan,Dan Lv,Kiyoshi Soyano,Kai Zhao
Bifenthrin (BF) is a broad-spectrum insecticide that has gained widespread use due to its high effectiveness. However, there is limited research on the potential toxic effects of bifenthrin pollution on amphibians. This study aimed to investigate the 50 % lethal concentration (LC 50 ) and safety concentration of Chinese giant salamanders (CGS) exposed to BF (at 0, 6.25,12.5,25 and 50 μg/L BF) for 96 h. Subsequently, CGS were exposed to BF (at 0, 0.04, and 4 μg/L BF) for one week to investigate its toxic effects. Clinical poisoning symptoms, liver pathology, oxidative stress factors, DNA damage, and transcriptome differences were observed and analyzed. The results indicate that exposure to BF at 4 μg/L significantly decreased the adenosine-triphosphate (ATP), superoxide dismutase (SOD), glutathione (GSH), and catalase (CAT) contents in the brain, liver, and kidney of CGS. Additionally, the study found that the malondialdehyde (MDA), reactive oxygen species (ROS), and 8-hydroxydeoxyguanosine (8-OHdG) contents were increased. The liver tissue exhibited significant inflammatory reactions and structural malformations. RNA-seq analysis of the liver showed that BF caused abnormal antioxidant indices of CGS. This affected molecular function genes such as catalytic activity, ATP-dependent activity, metabolic processes, signaling and immune system processes, behavior, and detoxification, which were significantly upregulated, resulting in the differential genes significantly enriched in the calcium signaling pathway, PPARα signaling pathway and NF-kB signaling pathway. The results suggest that BF induces the abnormal production of free radicals, which overwhelms the body's self-defense system, leading to varying degrees of oxidative stress. This can result in oxidative damage, DNA damage, abnormal lipid metabolism, autoimmune diseases, clinical poisoning symptoms, and tissue inflammation. This work provides a theoretical basis for the rational application of bifenthrin and environmental risk assessment, as well as scientific guidance for the conservation of amphibian populations.
研究领域:
环境毒理学
·
两栖生物学
·
分子生物学
·
生态毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）