Journal of Analytical Methods in Chemistry
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2022
·Vol. 2022
·期 1
·2565494
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IF 2.594
Qiulong Zhao,Chunxue Wang,Jiaxin Cheng,Hui Yan,Ling Wang,Dawei Qian,Jinao Duan
According to the sixth edition of China’s “New Coronavirus Diagnosis and Treatment Plan (NCDTP),” ReDuNing injection (RDN) was firstly introduced to treat severe and critical COVID-19, whereas its combination with broad-spectrum antibiotics was suggested to take with extreme caution and full reasons. Therefore, we aim to describe the pharmacokinetics of seven active phytochemicals and semiquantification of nine relevant metabolites in ReDuNing injection (RDN) after combining with cefuroxime sodium (CNa) for injection in rat plasma. Male Sprague–Dawley rats were randomly assigned to six groups, and they were intravenously administered, respectively, with different prescriptions of RDN (2 mL/kg) and CNa (225 mg/kg). At different time points (0.03, 0.08, 0.17, 0.24, 0.33, 0.50, 0.67, 1, and 6 h) after administration, the drug concentrations of iridoids glycosides, organic acids, and metabolites in rat plasma were determined using ultrahigh-pressure liquid chromatography coupled with linear ion rap-orbitrap tandem mass spectrometry (UHPLC–LTQ–Orbitrap–MS), and main pharmacokinetic parameters were estimated by noncompartment model. The results showed that there were differences in pharmacokinetic parameters, AUC (0-t) , T 1/2 , C max , CL of iridoids glycosides, and organic acids, after the intravenous administration of the different combinations of RDN and CNa. Moreover, different combinations of the injections also resulted in different curves of relative changes of each metabolite. The obtained results suggested that RDN and CNa existed pharmacokinetic drug–herb interactions in rats. The findings not only lay the foundation for evaluating the safety of RDN injection combined with CNa but also make contributions to clinically applying RDN injection combined with CNa, which works potentially against severe forms of COVID-19.
研究领域:
药理学
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药物代谢
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中药学
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临床药学
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分析化学
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病毒学
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转化医学
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JOURNAL OF MEMBRANE SCIENCE
·
2026
·
IF 8.8
Cheng Yi,Qian Liu,Tengfei He,Yue Yao,Lijia Liu,Chunhong Zhang,Chao Wang
Membranes with excellent chlorine resistance are required for stable operation and long service life. Traditional polyamide (PA) nanofiltration (NF) membranes typically suffer from inadequate chlorine resistance. To overcome this critical challenge and the long-standing problem of insufficient permeability, a novel polyesteramide (PEA) NF membrane was fabricated. Specifically, naringin (NG), a naturally occurring polyphenol rich in hydroxyl groups, was introduced as a co-monomer with piperazine (PIP) in the aqueous phase for interfacial polymerization with trimesoyl chloride (TMC) in the organic phase. The chlorine-susceptible amide groups were partially substituted by intrinsically chlorine-resistant ester groups in the fabricated PEA membrane. As a result, the chlorine resistance of the PEA membrane was greatly enhanced, with Na 2 SO 4 rejection remaining above 93 % after exposure to 20,000 ppm h of free chlorine. The resulting PEA membrane exhibited reduced surface roughness and selective-layer thickness, coupled with a slight increase in pore size and free volume fraction, which was attributed to intermolecular interactions between NG and PIP in the aqueous phase. Consequently, the PEA membrane demonstrated a water flux approximately twice that of the PA membrane, while maintaining a high Na 2 SO 4 rejection rate of 99 % and rejecting more than 98 % of various antibiotics (e.g., cephalosporins and tetracyclines). Furthermore, the PEA membrane exhibited favorable antifouling performance, satisfactory anti-swelling property, and filtration stability under various conditions.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2025
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IF 9
Chao Wang,Peijuan Chu,Tengfei He,Cheng Yi,Yue Yao,Lijia Liu,Chunhong Zhang
The application of membrane separation technology in wastewater treatment and pharmaceutical purification has attracted considerable attention. The development of a membrane with effective separation performance and antibacterial properties is of considerable interest for the separation and purification of antibiotics. Here, a thin-film composite (TFC) polyester membrane with effective antibiotic removal and antibacterial properties was fabricated via a facile one-step interfacial polymerization (IP) strategy. Naringenin (Nar), a natural product of flavonoid material derived from the pericarp, was utilized as the aqueous monomer. The suitable reactivity of Nar afforded the resulting membrane with a highly negative charged surface. The rejection performances for the antibiotics cephalothin, cefuroxime sodium, ceftriaxone sodium, and cefoperazone sodium were 96.3 %, 97.7 %, 99.2 %, and 99.8 %, respectively. The pure water permeance was 7.5 L m −2 h −1 bar −1 at a transmembrane pressure of 5 bar. The antibacterial efficacy was greater than 99.0 % against Escherichia coli and Staphylococcus aureus , attributable to the flavonoid structure of Nar. The present work proposed a feasible strategy to prepare TFC membranes with high nanofiltration performance and intrinsic antibacterial efficacy.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Hao-Jia Qin,Shuai Zhao,Hua-Ping Gong,Zhi Yu,Yufeng Wang,De Zhang,Pei Liang
Antibiotic residue detection plays a key role in ensuring human health. However, rapid, reliable, and highly sensitive detection methods are still insufficient. To tackle this challenge, in this study, the Au@Ag/Ce-UIO-66, a nanocomposite material consisting of bimetallic gold core and silver shell (Au@Ag) introduced on the surface of Ce-UIO-66, was successfully synthesized. By meticulously regulating the exact concentration and volume of silver nitrate, we successfully generated an optimal matrix. This significantly enhanced the detection capability of the probe molecule CV to an impressive concentration of 10 −9 M, with a remarkably low relative standard deviation of 7.54 %. The matrix exhibits excellent surface-enhanced Raman scattering (SERS) performance and stability, reaching adsorption equilibrium within 30 min. Moreover, employing this substrate enables the detection of sulfadiazine at concentrations as minimal as 2.5 μg/L, significantly surpassing the standards set by the European Union. Notably, the Raman spectral signal of sulfadiazine maintains stability across various pH conditions at a concentration of 10 −5 M. Finally, the adsorption mechanism and enhancement mechanism between Au@Ag/Ce-UIO-66 and sulfadiazine were explored.
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Cell Reports
·
2025
·
IF 6.9
Feiyu Yu,Shuyao Zhu,Qingyang Lv,Dan Fang,Haijie Zhang,Zhiqiang Wang,Yuan Liu
Tigecycline is a last-resort antibiotic primarily used to treat severe infections caused by multidrug-resistant (MDR) bacteria. However, the emergence of constantly appearing resistance determinants has drastically compromised its clinical effectiveness. Rational antibiotic combination provides a productive approach to tackle MDR pathogens. Herein, we show that evolved tigecycline-resistant Escherichia coli and Salmonella Enteritidis exhibit collateral sensitivity to aminoglycosides and nitrofurantoin. Proton motive force alterations mediate tigecycline resistance, simultaneously enhancing bacterial susceptibility to aminoglycosides. Notably, Lon dysfunction in tigecycline-resistant strains causes overexpression of sulA , increased lipopolysaccharide biosynthesis, and disrupted membrane homeostasis, leading to bacterial death upon nitrofurantoin exposure. The combination of tigecycline and nitrofurantoin effectively eradicates both evolved tigecycline-resistant bacteria and those carrying the tet (X4) gene while also hindering the development of tigecycline resistance. Together, our study suggests that combining tigecycline with existing antibiotics, rationally designed by harnessing collateral sensitivity, represents a promising therapeutic paradigm to combat tigecycline-resistant pathogens.
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PubMed
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Jiaxuan Liu,Wensi Ma,Xinyue Huang,Lei Jiang,Lin Yuan,Xiuling Ji,Yujiao Tu
Rapeseed oil residue (ROR) is the waste product of rapeseed oil extraction, mainly including protein and lignocellulose. In this study, we utilized waste ROR as the raw material to successfully prepare blue fluorescent carbon dots (ROR-CDs) by self-propagating synthesis. It demonstrated rapid preparation (20 min) with low energy consumption (400 °C) and without complicated purification processes. We analyzed the structural and optical properties of ROR-CDs. ROR-CDs exhibited exceptional fluorescence performance and stabilized, responding specifically to divalent mercury ions (Hg 2+ ) and aureomycin hydrochloride (CTC). Thus, we developed a high-sensitivity detection technique with a detection range and limit of detection (LOD) for Hg 2+ of 0–250 μg/L and 191.69 ng/L, respectively. For CTC, the detection range and LOD were 0–50 μM and 12.7 nM (S/N = 3), respectively. It is easy to operate and low-cost, ensuring efficient assistance for testing efforts in associated domains. Additionally, ROR-CDs-based detection technique can analyze Hg 2+ and CTC in the actual samples, demonstrating significant potential for application in environmental monitoring and food safety assessment.
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Frontiers in Cellular and Infection Microbiology
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2025
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IF 4.8
Jiansheng Lin,Chunyan Lin,Jingyang Zheng
Background: P. diazotrophicus was isolated from a newborn with D-galactosemia complicated sepsis. A homologous blaCAR-2 gene, encoding CAR-2, a predicted member of the CAR family subclass B3 metallo-β-lactamases (MBLs), was found in the genome of this strain. This study aimed to explore the identification of a novel CAR-2 protein encoded by the chromosome of P. diazotrophicus Pd1 that exhibits the zinc-binding motifs of subclass B3 enzymes and the regulatory pattern of CARR, located directly upstream of the blaCAR-2 gene, on the blaCAR-2 gene and its impact on antibiotic resistance.Methods: Antibiotic susceptibility testing was conducted by the plate agar dilution method. Site-directed mutagenesis was conducted using Mut Express II Fast Mutagenesis Kit V2. Kinetic assays were used to determine the hydrolysis of β-Lactam. The construction of bacterial knockout strains was carried out according to the principle of homologous recombination. The detection of the mRNA expression level of the gene was performed by Real-time Quantitative PCR (qPCR).Results: The minimum inhibitory concentrations (MICs) for E.coli DH5α(pHSG398::CAR-2), which expressed blaCAR-2, increased significantly for cefalothin, cefuroxime and cefotaxime sodium by 2-, 16- and 32- fold, respectively, which showed that blaCAR-2 had resistance to these three antibiotics. This protein, which was a MBL, contains two classical zinc-binding sites characteristic of subclass B3, with the amino acid motif His136, His138, His211, Asp140, His141, and His276. Among the six residues, His136, Asp140, and His211 exhibited the highest catalytic activity. We determined that CAR-2 can effectively hydrolyze cefalothin, cefuroxime and cefotaxime. The chloramphenicol resistance of the constructed E. coli DH5α strain was significantly reduced in the presence of CARR than in the absence of CARR. Compared with those for the wild-type P. diazotrophicus, MICs of cefalothin, cefuroxime and cefotaxime for the ΔCARR strain increased by 8-, 8- and 16-fold, respectively, and the expression level of blaCAR-2 also increased by approximately 10-fold.Conclusion: Overall, CAR-2 is a novel subclass B3 MBL of the CAR family that exhibits catalytic activity against cefalothin, cefuroxime and cefotaxime. CARR represses the expression of blaCAR-2, thereby reducing the resistance of P. diazotrophicus to these antibiotics. This provided a theoretical basis for revealing new mechanisms of pathogen resistance.
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PubMed
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