查找使用阿拉丁产品的科研文献

In-situ loading of ag nanoparticles and sodium cefoperazone onto bacterial cellulose-based composite antimicrobial films for promoting wound healing

Xinru Wang,Jie Zhang,Jinyu Shan,Dong Yang,Guangming Tian,Yunzhi Dang,Jianhua Ma

This study focuses on bacterial cellulose (BC), a material with a three-dimensional network structure, excellent biocompatibility, and superior mechanical properties, to address its inherent limitations of insufficient antibacterial activity and poor rehydration capability. Silver nanoparticles (AgNPs) were synthesized in situ on the BC surface via a hydrothermal method, followed by immersion in polyethylene glycol (PEG) and cefoperazone sodium (CEFNa) solutions to prepare BC/Ag/PEG/CEFNa composite films. The BC films and composites were comprehensively characterized using techniques such as XRD, SEM, and FTIR spectroscopy. Systematic characterization results revealed that the introduction of PEG not only increased the film's moisture absorption and water vapor permeability by 2.8 and 1.7 times, respectively, but also enhanced the CEFNa loading capacity to 1.6 times that of pure BC. Antibacterial experiments demonstrated that while pure BC films exhibited no antibacterial activity, the composite films showed inhibition zone diameters of 15.5 mm, 17.5 mm, and 12.3 mm against Escherichia coli , Staphylococcus aureus , and Pseudomonas aeruginosa , respectively. Mouse wound healing experiments indicated that the wound healing rate of the composite film treatment group was significantly improved by 21.9 % compared to the pure BC group after 15 days. These results demonstrate that the prepared BC/Ag/PEG/CEFNa composite films possess excellent physicochemical properties, significant antibacterial activity, and remarkable wound-healing effects, highlighting their great potential as advanced wound dressings for skin injury repair.

Light-Activable Inhibitor Overcomes Antimicrobial Resistance and Regulates Antibacterial Activity

Zhuang Ma,Boxuan Hou,Anhui Liao,Ying Tan,Chunyan Tan,Yuyang Jiang

Overuse of antibiotics and the widespread environmental accumulation of antibiotics drive the evolution and spread of antimicrobial resistance, posing a significant global health threat by reducing the effectiveness of available treatments and increasing the risk of untreatable infections. We designed and synthesized PhoPS, a novel photocaged β-lactamase inhibitor, which incorporates the pharmacophore of sulbactam caged with a photoresponsive moiety of o-nitrobiphenyl derivative. Experimental results demonstrate its rapid photoactivation, good stability in solution, and light-activated β-lactamase inhibition in vitro. PhoPS displays synergy with a cephalosporin antibiotic cefoperazone against both susceptible and resistant strains of Escherichia coli and biofilm formation. Additionally, PhoPS treatment demonstrates the potential to suppress the development of resistance in E. coli. These findings suggest that PhoPS offers a promising approach for restoring the efficacy of existing antibiotics and mitigating the emergence of AMR.

Halloysite nanotubes-based hybrid silica monolithic spin tip for hydrophilic solid-phase extraction of sulbactam, cefoperazone, and cefuroxime in whole blood

Rongrong Xuan,Bingye Shi,Dongchen Li,Yihui Chen,Chunyan Hou,Rufeng Jiang,Mengyue Guo,Yongyan Zhang,Tingting Wang

In this study, we proposed a novel method utilizing polyethyleneimine (PEI)-modified halloysite nanotubes (HNTs)-based hybrid silica monolithic spin tip to analyze hydrophilic β-lactam antibiotics and β-lactamases inhibitors in whole blood samples for the first time. HNTs were incorporated directly into the hybrid silica monolith via a sol-gel method, which improved the hydrophilicity of the matrix. The as-prepared monolith was further modified with PEI by glutaraldehyde coupling reaction. It was found that the PEI-modified HNTs-based hybrid silica monolith enabled a large adsorption capacity of cefoperazone at 35.7 mg g −1 . The monolithic spin tip-based purification method greatly reduced the matrix effect of whole blood samples and had a detection limit as low as 0.1 − 0.2 ng mL −1 . In addition, the spiked recoveries of sulbactam, cefuroxime, and cefoperazone in blank whole blood were in the range of 89.3–105.4 % for intra-day and 90.6–103.5 % for inter-day, with low relative standard deviations of 1.3–7.2 % and 4.9–10.5 %, respectively. This study introduces a new strategy for preparing nanoparticles incorporated in a hybrid silica monolith with a high adsorption capacity. Moreover, it offers a valuable tool to monitor sulbactam, cefoperazone, and cefuroxime in whole blood from pregnant women with the final aim of guiding their administration.

Mucus-Penetrable Biomimetic Nanoantibiotics for Pathogen-Induced Pneumonia Treatment

Yue Wang,Qihang Ding,Gongcheng Ma,Zhiwei Zhang,Jiaqi Wang,Chang Lu,Chunbai Xiang,Kun Qian,Jun Zheng,Yaming Shan,Pengfei Zhang,Zhen Cheng,Ping Gong,Qi Zhao

Bacterial pneumonia has garnered significant attention in the realm of infectious diseases owing to a surge in the incidence of severe infections coupled with the growing scarcity of efficacious therapeutic modalities. Antibiotic treatment is still an irreplaceable method for bacterial pneumonia because of its strong bactericidal activity and good clinical efficacy. However, the mucus layer forming after a bacterial infection in the lungs has been considered as the “Achilles’ heels” facing the clinical application of such treatment. Herein, traceable biomimetic nanoantibiotics (BioNanoCFPs) were developed by loading indacenodithieno[3,2-b]thiophene (ITIC) and cefoperazone (CFP) in nanoplatforms coated with natural killer (NK) cell membranes. The BioNanoCFP exhibited excellent demonstrated mucus-penetrating abilities, facilitating their arrival at the infection site. The presence of Toll-like receptors in the NK cell membrane rendered the BioNanoCFP with the capability to recognize pathogen-associated molecular patterns within bacteria, allowing precise targeting of bacterial colonization sites and achieving substantial therapeutic efficacy. Overall, our findings demonstrate the viability and desirability of using NK cell membrane-mediated drug delivery as a promising strategy for precision treatment.

Extracellular vesicles enhance the efficacy of ceftiofur against intracellular bacterial infections

Pingping Yuan,Shijie Zhou,Qianqian Li,Lin Li,Shaoqi Qu

Bacterial infections pose a major threat to human health and economic stability. In particular, intracellular bacterial infections present significant clinical challenges due to antibiotic resistance and poor drug penetration. Therefore, there is an urgent need to develop novel therapeutic strategies to address the problem of intracellular bacterial infections. Extracellular bacterial vesicles become ideal delivery systems due to their natural targeting properties. Here, we developed a bacteria-derived extracellular vesicles (EVs)-based drug delivery platform to enhance the therapeutic efficacy of antibiotics against intracellular infections. EVs were successfully isolated and increased production from S. aureus by ultracentrifugation, then loaded with ceftiofur (CEF) via co-incubation. In vitro tests demonstrated the potent antibacterial activity of CEF, achieving complete growth inhibition within 24 h and a 4-log viability reduction in 4 h. Furthermore, confocal microscopy revealed efficient CEV internalization in IEC-6 cells, with 12-fold greater intracellular bacterial clearance than free CEF. In vivo , CEV-incorporated hydrogel (CEVH) significantly reduced both intra- and extracellular bacterial loads and accelerated wound healing. These findings demonstrate that bacterial EVs serve as a universal delivery platform to significantly enhance the efficacy of existing antibiotics against intracellular infections.

A halloysite nanotubes-based hybrid fluorous monolith as the mixed-mode sorbent for hydrophilic and hydrophobic interactions and its application for analysis of antibiotics from milk powder and milk samples

Cheng Lin,Jiarun Mao,Yihui Chen,Chunyan Hou,Xiaoqiang Qiao,Ningkun Wu,Tingting Wang

Determining the hydrophilic and hydrophobic antibiotics in milk and related products poses significant challenges due to their polarity differences and trace levels. Herein, we proposed a halloysite nanotubes-based hybrid fluorous monolith (HNTs-PFOTS monolith) as a sorbent for spin-tip solid-phase extraction (SPE). Leveraging the perfluorooctyl groups of the HNTs-PFOTS monolith, antibiotics can be adsorbed through hydrophilic and hydrophobic interactions. An analytical method was developed by combining the HNTs-PFOTS monolith-based spin-tip SPE method with liquid chromatography-tandem mass spectrometry. Under optimized conditions, the method exhibited excellent purification capabilities, low detection limits (0.01–0.03 μg/kg), high recoveries (80.0–112.3 %), and satisfactory reproducibility (intra-day RSDs of 0.4–8.8 % and inter-day RSDs of 1.0–10.9 %) for hydrophilic and hydrophobic antibiotics in milk and milk product samples. This study offers a novel approach for developing sorbents targeting antibiotics and provides a new analytical method for determining hydrophilic and hydrophobic antibiotics in food products.

A highly sensitive electrochemical sensor derived from peptide amphiphilic inspired self-assembled, ordered gold nanoparticles for determination of 22 β-lactams

Yexuan Mao,Yichao Fan,Ran Yang,Youyi Wang,Qingyue Li,Meng Dang,Xianqing Huang,Lianjun Song,Pingan Zhang,Miao Song,Liang Liu,Linli Wang,Shun Yu,Qiuyan Zhao,Xiya Zhang

Achieving ordered arrangement of gold nanoparticles (AuNPs) on an electrode can further improve the signal intensity of an electrochemical immunosensor. This ordered AuNPs self-assembly can be inspired by peptide amphiphile (PA). The rational design of hydrophobic chains in PA is a crucial step when the range of hydrophobic alkane chain from 14 to 18 carbons. As arginine (R) can combine specifically with Au surface plane (111), four PAs, i.e., C 15 H 23 CO-RR (C16R2), C 15 H 23 CO-RRR (C16R3), C 15 H 23 CO-RRRR (C16R4), and C 15 H 23 CO-RRRRR (C16R5), in this work are designed to investigate the extent of ordered AuNPs self-assembly. The results indicate that C16R4 presented a dominant electron donating ability and can inspire the self-assembly of AuNPs to form a mono-dispersed raspberry-like structure (C16R4-AuNPs) to obtain the largest ΔI (15.4 μA). In the detection system, C16R4-AuNPs firstly capture and immobilize anti-β-lactam receptor monoclonal antibodies (mAb) by electrostatic adsorption, and the anti-β-lactam receptor mAb possess antigen-antibody binding sites away from the active site of the receptors. Then, they capture β-lactam receptors through an antigen-antibody reaction to form a ternary complex (C16R4-AuNPs-mAb-β-lactam receptors). Based on this indirect-labeling process, the receptor active sites have been exposed completely, facilitating ligand recognition, which greatly further improves the sensitivity of the present method. Finally, a highly sensitive electrochemical immunosensor based on the ternary complex is developed to achieve the determination of 22 β-lactam antibiotics, which is more sensitive and applicable than other reported electrochemical sensors.

High-performance Hf/Ti-doped defective Zr-MOFs for cefoperazone adsorption: Behavior and mechanisms

Menghan Qin,Yumiao Shi,Dingkun Lu,Jingjing Deng,Guoyue Shi,Tianshu Zhou

Due to the excessive use, cephalosporin antibiotics have been detected in various aquatic environments, which may result in environmental and health concern. In this study, bimetallic metal–organic frameworks (MOFs), Hf-UiO-66 and Ti-UiO-66, were successfully designed and obtained via post-synthetic method from UiO-66 for the adsorption of cefoperazone. A serious of adsorption experiments were investigated. The adsorption kinetics and isotherms demonstrated that the adsorption process followed pseudo-second-order kinetic model and Langmuir isotherm model. Hf/Ti-UiO-66 exhibited much better adsorption performance than the undoped UiO-66. Particularly, Hf-UiO-66 had the highest adsorption capacity (346.0 mg g −1 ). With the introduction of Hf or Ti, the chemical coordination environment changed, which resulted in defective structures of MOFs. Such defective structures increased the adsorption active sites, changed the surface charges and enlarged pores. Based on the characterization by FT-IR, XPS, and zeta potential, it was identified that the electrostatic attraction, π-π stacking, hydrogen bonding and metal complexation were the driving force for the high adsorption capacity of the bimetallic MOFs. The as-prepared MOFs displayed good stability and reusability. This work provides a great prospect for the design and application of bimetallic defective MOFs in cephalosporins removal in environmental water.

Rapid Determination of Cephalexin in Animal-Derived Food by an Indirect Competitive Enzyme-Linked Immunosorbent Assay

Shuangmin Wu,Wei Shen,Yushuang Chen,Yongli Zhu,Ren Hou,Menghong Dai,Dapeng Peng