查找使用阿拉丁产品的科研文献

l-Cysteine directing synthesis of BiOBr nanosheets for efficient cefazolin photodegradation: The pivotal role of thiol

Yingying Chen,Hailin Tian,Wenxue Zhu,Xin Zhang,Ruiping Li,Chuncheng Chen,Yingping Huang

Cefazolin (CFZ) is widely present in the wastewater treatment effluents and in receiving waters and has caused severe impacts to the ecosystem. CFZ degradation by photocatalysis has attracted increasing attention due to its eco-friendly features. Herein, we presented a green synthesis strategy for a highly active BiOBr photocatalyst for CFZ removal with L -cysteine as a directing agent, and the role of thiol in cysteine for facets control and morphology regulation was discussed. We found that the photoactivity of cysteine-induced BiOBr nanosheet was much higher than those prepared by using arginine and glycine as directing agent. Further experiments showed that the cysteine preferentially coordinated Bi 3+ with thiol rather than carboxyl. The strong interactions of thiol group with the external surface of the BiOBr crystals stabilize the small crystals that have high surface energy without the cysteine. Such a chemical environment favors forming BiOBr crystalline with small size of high surface area and oriented growth in [110] direction, which facilitates the photogenerated electron-hole separation to achieve significantly promoted photocatalytic activity. Moreover, the cysteine-directed BiOBr nanosheets displayed good photoreactivity to more pollutants (i.e. rhodamine B, cefradine and cefoperazone sodium) and excellent reusability as verified via seven consecutive recycle experiments. The proper photocatalyst dosage (0.4 g/L), relatively acidic water environment (pH 3.4) and high temperature (35 °C) would be beneficial to CFZ photodegrdation by BiOBr. Also, the CFZ photodegradation mechanism and degradation pathway were also proposed by combining radical trapping experiments and electron spin resonance (ESR) test with LC-MS analysis in the photocatalytic process.

Photocatalytic removal of cefazolin using Ag3PO4/BiOBr under visible light and optimization of parameters by response surface methodology

Yuhui Xiao,Xianghua Song,Zhuo Liu,Ruiping Li,Xiaorong Zhao,Yingping Huang

The degradation of cefazolin (CFZ) by Ag 3 PO 4 /BiOBr composites under visible-light irradiation was explored. The main and interaction of parameters (catalyst dosage, pH, CFZ initial concentration and degradation time) on removal of CFZ were studied by Box–Behnken design combined with response surface methodology. The pH was the most influential factor and both h + and OH played a role in the photocatalytic process. The high correlation coefficients (R 2   =   0.9986 and adjusted R 2   =   0.9973) demonstrated close fit between the predicted and experimental values. The exceptional efficiency of Ag 3 PO 4 /BiOBr composite in removing CFZ represents a promising technique for treatment of CFZ-containing wastewater.

Tuning monomer spatial configuration for high-performance polyesteramide loose nanofiltration membranes toward antibiotic separation

Zhixiao Liu,Yuhang Zhao,Mingli Yin,Jiaxu Wan,Yangyang Huang,Guangqiu Lei,Kangfei Wang,Tianliang Zhu,Zhengyu Qiu,Xiaoran Zhang,Zhiming Mi

Polyesteramide (PEA) thin-film composite (TFC) membranes have emerged as promising candidates for antibiotic separation from water, yet rational monomer design to overcome the permeability-selectivity trade-off remains challenging. Herein, three tetrafunctional phenol-amine monomers with distinct spatial configurations—rigid twisted 2,2-bis(3-amino-4-hydroxyphenyl)propane (BAP), rigid rotatable 3,3-dihydroxybenzidine (HAB), and bulky side-group-containing 9,9-bis(3-amino-4-hydroxyphenyl)fluorene (BAF)—were respectively reacted with trimesoyl chloride via interfacial polymerization to fabricate novel PEA loose nanofiltration (LNF) membranes. Monomer configuration governed membrane morphology, extent of crosslinking reaction, pore architecture, and surface properties. DFT calculations and UV-Vis diffusion kinetics elucidated monomer reactivity and diffusion behavior. Among the three membranes, NF-HAB, derived from HAB, demonstrated the optimal overall performance. It achieved a high water permeance of 50.5 L m −2  h −1 ·bar −1 while maintaining excellent rejection rates of both weakly negative antibiotics (e.g., 92.0% for cefoperazone) and their negatively charged sodium salts (e.g., >96% for ceftriaxone sodium and cefoperazone sodium). Furthermore, all PEA membranes exhibited superior chlorine resistance and antifouling performance. This work establishes a structure-property-mechanism relationship, demonstrating that tuning monomer spatial configuration enables synergistic optimization of permeance and selectivity for high-performance PEA LNF membranes in antibiotic separation.

Closed-loop theranostic hydrogel-electronics patch for chronic wound management

Lin Kanglong,Yang Baili,Yan Guizhi,Li Changhao,Zhao Guoxu,Zou Yang,Li Zhou,Wang Dong

Chronic diabetic wounds present a significant clinical challenge due to antibiotic abuse and limited understanding of the wound microenvironment. Real-time monitoring of key biomarkers in the wound microenvironment can provide critical insights into wound status and guide therapeutic interventions. In this study, we developed an intelligent wound patch by integrating a near-infrared (NIR)-responsive photothermal antibiotic-releasing hydrogel (PPQTA) with a multichannel electrochemical biosensor array, which takes sensor monitoring-guided precise drug administration as its core. The sensing module can continuously monitor the key biomarkers in the wound in situ with high sensitivity and strong anti-interference capability within the physiological range (pH: 4–9; uric acid: 0–700 μM; glucose: 5–30 mM), providing a quantitative basis for drug release. The PPQTA hydrogel can release drugs on demand via NIR irradiation based on the sensing results, achieving 100% antibacterial efficacy against both Gram-negative and Gram-positive bacteria. Experiments in a diabetic rat wound model confirmed that this patch can monitor the dynamic changes of biomarkers in situ and guide drug administration within 7 days. After terminating drug administration on day 4, the bacteria in the wound were effectively eliminated on day 5, and the wound healing process was significantly promoted. By combining real-time diagnostics with responsive therapy, this patch offers a closed-loop strategy for personalized management of diabetic chronic wounds.

Rapeseed oil residue-based fluorescent probes for bifunctional detection of Hg2+ and aureomycin hydrochloride

Jiaxuan Liu,Wensi Ma,Xinyue Huang,Lei Jiang,Lin Yuan,Xiuling Ji,Yujiao Tu

Rapeseed oil residue (ROR) is the waste product of rapeseed oil extraction, mainly including protein and lignocellulose. In this study, we utilized waste ROR as the raw material to successfully prepare blue fluorescent carbon dots (ROR-CDs) by self-propagating synthesis. It demonstrated rapid preparation (20 min) with low energy consumption (400 °C) and without complicated purification processes. We analyzed the structural and optical properties of ROR-CDs. ROR-CDs exhibited exceptional fluorescence performance and stabilized, responding specifically to divalent mercury ions (Hg 2+ ) and aureomycin hydrochloride (CTC). Thus, we developed a high-sensitivity detection technique with a detection range and limit of detection (LOD) for Hg 2+ of 0–250 μg/L and 191.69 ng/L, respectively. For CTC, the detection range and LOD were 0–50 μM and 12.7 nM (S/N = 3), respectively. It is easy to operate and low-cost, ensuring efficient assistance for testing efforts in associated domains. Additionally, ROR-CDs-based detection technique can analyze Hg 2+ and CTC in the actual samples, demonstrating significant potential for application in environmental monitoring and food safety assessment.

Design and application of metal-organic framework based chemo-fluorescent sensor for selective sensing of the full spectrum of tetracyclines in complex samples

Yaowei Zhu,Ruijie Tang,Jun Zhao,Changlong Jiang,Lingfei Li,Bianhua Liu

Tetracyclines antibiotics (TCs) are widely used as broad-spectrum antibiotics, but their overuse leads to environmental pollution and threatens human health. Existing detection techniques (mass spectrometry, etc.) are complicated and cumbersome, and do not allow for rapid detection. Consequently, it is imperative to develop a sensing technology for rapid on-site detection of full-spectrum TCs residues. The UV–visible absorption peaks of TCs overlap with the blue fluorescence excitation wavelength range, which can “turn-off” the blue fluorescence based on the internal filtering effect. Additionally, the β-diketone bond of TCs can bind to Eu 3+ , sensitizing Eu 3+ to produce red fluorescence. Thus, we constructed a selective sensing chemo-fluorescent sensor using blue nitrogen-doped carbon dots (BCDs) and europium metal–organic framework (Eu-MOF). As the concentration of TCs increased from 0 to 2.6 μM, the sensor exhibited a continuous change in fluorescence color from blue to purple to red. The limit of detection is 1.37 nM with a response time of less than 1 s. Furthermore, this study presents a portable capillary chemo-fluorescent sensor detection kit, which consists of a capillary chemo-fluorescent sensor comprising BCDs/Eu-MOF encapsulated in a self-designed non-fluorescent glass capillary, as well as a color recognizer with a kit produced using 3D printing technology. This fluorescence sensing technology with excellent sensitivity and selectivity provides technical support for the rapid detection of TCs and the reduction of pollutant hazards to sustainable human development.

Degradation of β-lactam antibiotics by Fe(III)/HSO3− system and their quantitative structure-activity relationship

Zhenbin Chen,Haohan Cai,Feng Huang,Zongping Wang,Yiqun Chen,Zizheng Liu,Pengchao Xie

β-lactam antibiotics, extensively used worldwide, pose significant risks to human health and ecological safety due to their accumulation in the environment. Recent studies have demonstrated the efficacy of transition metal-activated sulfite systems, like Fe(Ⅲ)/HSO 3 − , in removing PPCPs from water. However, research on their capability to degrade β-lactam antibiotics remains sparse. This paper evaluates the degradation of 14 types of β-lactam antibiotics in Fe(Ⅲ)/HSO 3 − system and establishes a QSAR model correlating molecular descriptors with degradation rates using the MLR method. Using cefazolin as a case study , this research predicts degradation pathways through NPA charge and Fukui function analysis, corroborated by UPLC-MS product analysis. The investigation further explores the influence of variables such as HSO 3 − dosage, substrate concentration , Fe(Ⅲ) dosage, initial pH and the presence of common seen water matrices including humic acid and bicarbonate on the degradation efficiency. Optimal conditions for cefazolin degradation in Fe(Ⅲ)/HSO 3 − system were determined to be 93.3 μM HSO 3 − , 8.12 μM Fe(Ⅲ) and an initial pH of 3.61, under which the interaction of Fe(Ⅲ) dosage with initial pH was found to significantly affect the degradation efficiency. This study not only provides a novel degradation approach for β-lactam antibiotics but also expands the theoretical application horizon of the Fe(Ⅲ)/HSO 3 − system.

Preparation of visible-light active MOFs-Perovskites (ZIF-67/LaFeO3) nanocatalysts for exceptional CO2 conversion, organic pollutants and antibiotics degradation

Aftab Khan,Samreen Sadiq,Iltaf Khan,Muhammad Humayun,Guo Jiyuan,Muhammad Usman,Abbas Khan,Shoaib Khan,Amal Faleh Alanazi,Mohamed Bououdina

Modern industries rapid expansion has heightened energy needs and accelerated fossil fuel depletion, contributing to global warming. Additionally, organic pollutants present substantial risks to aquatic ecosystems due to their stability, insolubility, and non-biodegradability. Scientists are currently researching high-performance materials to address these issues. LaFeO 3 nanosheets (LFO-NS) were synthesized in this study using a solvothermal method with polyvinylpyrrolidone (PVP) as a soft template. The LFO-NS demonstrate superior performance, large surface area and charge separation than that of LaFeO 3 nanoparticles (LFO-NP). The LFO-NS performance is further upgraded by incorporating ZIF-67. Our results confirmed the ZIF-67/LFO-NS nanocomposite have superior performances than pure LFO-NP and ZIF-67. The integration of ZIF-67 has enhanced the charge separation and promote the surface area of LFO-NSwhich was confirmed by various characterization techniques including TEM, HRTEM, DRS, EDX, XRD, FS, XPS, FT-IR, BET, PL, and RAMAN. The 5ZIF-67/LFO-NS sample showed significant activities for CO 2 conversion, malachite green degradation, and antibiotics (cefazolin, oxacillin, and vancomycin) degradation. Furthermore, stability tests have confirmed that our optimal sample very active and stable. Furthermore, based on scavenger experiments and the photocatalytic degradation pathways, it has been established that H + and •O 2 − are vital in the decomposition of MG and antibiotics. Our research work will open new gateways to prepare MOFs-Perovskites nanocatalysts for exceptional CO 2 conversion, organic pollutants and antibiotics degradation.

Rapid Determination of Cephalexin in Animal-Derived Food by an Indirect Competitive Enzyme-Linked Immunosorbent Assay

Shuangmin Wu,Wei Shen,Yushuang Chen,Yongli Zhu,Ren Hou,Menghong Dai,Dapeng Peng

The Lysine Acetylation Modification in the Porin Aha1 of Aeromonas hydrophila Regulates the Uptake of Multidrug Antibiotics

Lishan Zhang,Zujie Yao,Huamei Tang,Qingli Song,Huanhuan Song,Jindong Yao,Zhen Li,Xiaofang Xie,Yuexu Lin,Xiangmin Lin

Protein lysine acetylation (Kac) modification plays important roles in diverse physiological functions. However, there is little evidence on the role of Kac modification in bacterial antibiotic resistance. Here, we compared the differential expressions of whole-cell proteins and Kac peptides in oxytetracycline sensitive and oxytetracycline resistance (OXYR) strains of Aeromonas hydrophila using quantitative proteomics technologies. We observed a porin family protein Aha1 downregulated in the OXYR strain, which may have an important role in the OXY resistance. Interestingly, seven of eight Kac peptides of Aha1 decreased abundance in OXYR as well. Microbiologic assays showed that the K57R, K187R, and K197R Aha1 mutants significantly increased antibiotic resistance to OXY and reduced the intracellular OXY accumulation in OXY stress. Moreover, these Aha1 mutants displayed multidrug resistance features to tetracyclines and β-lactam antibiotics. The 3D model prediction showed that the Kac states of K57, K187, and K197 sites located at the extracellular pore vestibule of Aha1 may be involved in the uptake of specific types of antibiotics. Overall, our results indicate a novel antibiotic resistance mechanism mediated by Kac modification, which may provide a clue for the development of antibiotic therapy strategies.

A highly sensitive electrochemical sensor derived from peptide amphiphilic inspired self-assembled, ordered gold nanoparticles for determination of 22 β-lactams

Yexuan Mao,Yichao Fan,Ran Yang,Youyi Wang,Qingyue Li,Meng Dang,Xianqing Huang,Lianjun Song,Pingan Zhang,Miao Song,Liang Liu,Linli Wang,Shun Yu,Qiuyan Zhao,Xiya Zhang

Achieving ordered arrangement of gold nanoparticles (AuNPs) on an electrode can further improve the signal intensity of an electrochemical immunosensor. This ordered AuNPs self-assembly can be inspired by peptide amphiphile (PA). The rational design of hydrophobic chains in PA is a crucial step when the range of hydrophobic alkane chain from 14 to 18 carbons. As arginine (R) can combine specifically with Au surface plane (111), four PAs, i.e., C 15 H 23 CO-RR (C16R2), C 15 H 23 CO-RRR (C16R3), C 15 H 23 CO-RRRR (C16R4), and C 15 H 23 CO-RRRRR (C16R5), in this work are designed to investigate the extent of ordered AuNPs self-assembly. The results indicate that C16R4 presented a dominant electron donating ability and can inspire the self-assembly of AuNPs to form a mono-dispersed raspberry-like structure (C16R4-AuNPs) to obtain the largest ΔI (15.4 μA). In the detection system, C16R4-AuNPs firstly capture and immobilize anti-β-lactam receptor monoclonal antibodies (mAb) by electrostatic adsorption, and the anti-β-lactam receptor mAb possess antigen-antibody binding sites away from the active site of the receptors. Then, they capture β-lactam receptors through an antigen-antibody reaction to form a ternary complex (C16R4-AuNPs-mAb-β-lactam receptors). Based on this indirect-labeling process, the receptor active sites have been exposed completely, facilitating ligand recognition, which greatly further improves the sensitivity of the present method. Finally, a highly sensitive electrochemical immunosensor based on the ternary complex is developed to achieve the determination of 22 β-lactam antibiotics, which is more sensitive and applicable than other reported electrochemical sensors.