NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 37
·17293-17302
·
IF 3.3
Tong Shao,Xiaoshuang Wang,Rentian Guan,Suyuan Zeng,Rui Li,Min Hong,Qiaoli Yue
In this study, carbonized polymer dots (CPDs) were first proposed for the quantitative detection of chlortetracycline (CTC) based on aggregation induced emission. CPDs were synthesized by a one-step hydrothermal assay using polyethylene imine as the sole precursor. CPDs showed bright blue fluorescence under UV light (λEx 350 nm and λEm 465 nm), with a quantum yield of 14.58%. There was a significant fluorescence enhancement for the CPDs + CTC system. When the concentration of CTC increased, the fluorescence color of CPDs displayed a significant change from bright blue to dark blue with the maximum emission shifting from 465 nm to 430 nm. Furthermore, the lifetime of CPDs + CTC increased from 7.12 ns to 11.26 ns, an increment of 4.14 ns, which was ascribed to the aggregation induced emission of CTC in the presence of CPDs. There was a good linear response between the fluorescence intensity of CPDs and the concentration of CTC from 0.1 μM to 120 μM with a limit of detection of 40 nM. Therefore, an excellent fluorescence probe was successfully prepared for the detection of CTC and its application in real samples was confirmed.
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高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Wenhui Qiu,Xinjie Liu,Feng Yang,Rongzhen Li,Ying Xiong,Caixia Fu,Guanrong Li,Shuai Liu,Chunmiao Zheng
In China, antibiotics are commonly used for human and veterinary medicine, and they are present in various environmental media. Thus, the toxic effects of antibiotics on organisms have attracted the attention of society and scientists alike. In this study, zebrafish embryos were used to test the single and joint toxicity of four antibiotics, sulfamonomethoxine (SMM), cefotaxime sodium (CFT), tetracycline (TC), enrofloxacin (ENR), and their combinations, combining the results of experimental and omics techniques. Following exposure to antibiotics for 120 h, the body lengths of zebrafish larvae in all 100 μg/L antibiotic groups were significantly shortened, and the reactive oxygen species (ROS) content in the 100 μg/L Mix group was significantly increased. Transcriptome sequencing (RNA-seq) showed that the mRNA level of numerous genes was significantly changed in the five antibiotic treatment groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of the differentially expressed genes revealed a significant enrichment of the steroid biosynthesis and other metabolism pathways. Hub gene analysis highlighted dhcr24 , acat1 , aldh1a2 , aldh8a1 , suclg2 , hadh , and hsdl2 as the key genes, and hub gene expression changes because of the antibiotic treatment suggested that the metabolic system of the zebrafish larvae was severely disrupted by the interaction with other genes. In conclusion, single or joint exposure to different antibiotics at environmental concentrations affected the early development and metabolic system of zebrafish larvae, and our results provide fundamental evidence for future studies of antibiotic toxicity in aquatic organisms.
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PubMed
高影响因子
Microorganisms
·
2024
·Vol. 12
·期 4
·675
·
IF 4.1
Huizhong Pang,Kaixuan Zheng,Wenbo Wang,Mingjuan Zheng,Yudan Liu,Hong Yin,Daochuan Zhang
Cefotaxime (CTX) is an easily detectable antibiotic pollutant in the water environment, but little is known about its toxic effects on aquatic invertebrates, especially on the intestine. Here, we determined the oxidative stress conditions ofA. sinicaunder CTX exposure with five concentrations (0, 0.001, 0.01, 0.1 and 1 mg/L) for 14 days. After that, we focused on changes in intestinal tissue morphology and gut microbiota inA. sinicacaused by CTX exposure at 0.01 mg/L. We found malondialdehyde (MDA) was elevated in CTX treatment groups, suggesting the obvious antibiotic-induced oxidative stress. We also found CTX exposure at 0.01 mg/L decreased the villus height and muscularis thickness in gut tissue. The 16S rRNA gene analysis indicated that CTX exposure reshaped the gut microbiota diversity and community composition. Proteobacteria, Actinobacteriota and Bacteroidota were the most widely represented phyla inA. sinicagut. The exposure to CTX led to the absence of Verrucomicrobia in dominant phyla and an increase in Bacteroidota abundance. At the genus level, eleven genera with an abundance greater than 0.1% exhibited statistically significant differences among groups. Furthermore, changes in gut microbiota composition were accompanied by modifications in gut microbiota functions, with an up-regulation in amino acid and drug metabolism functions and a down-regulation in xenobiotic biodegradation and lipid metabolism-related functions under CTX exposure. Overall, our study enhances our understanding of the intestinal damage and microbiota disorder caused by the cefotaxime pollutant in aquatic invertebrates, which would provide guidance for healthy aquaculture.
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PubMed
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Xiaoqi Jiang,Jingcheng Huang,Mengting Li,Linjin Li,Yinuo Chen,Jiangfei Chen,Junlun Zhu,Xuedong Wang,He Mei
Detecting tetracyclines' (TC) residues is of great significance for evaluating the food and environmental safety. Herein, we pioneered a reversible-responsive ratiometric fluorometry by integrating the red fluorescence of Eu 3+ /GMP lanthanide coordination polymers (Eu 3+ /GMP LCPs) with the blue fluorescence of an anthracene-based imidazolium ionic liquid (ABIIL). In the presence of TC, the fluorescence of ABIIL was quenched by TC via the inner-filter effect and photoinduced electron-transfer process, but that of Eu 3+ /GMP LCPs was enhanced owing to the antenna effect between Eu 3+ and TC. Based on the reversible variations of the fluorescence signals, a fluorescent assay based on ABIIL-Eu 3+ /GMP LCPs was engineered to detect TC with satisfactory sensitivity and selectivity. A good linearity for TC was acquired from 0 to 50 μM with the detection limit of 3.0 nM and the significant color changes. Therefore, the assay was a promising approach for the visual detection of TC in complex media
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PubMed
高影响因子
Biomolecules
·
2025
·Vol. 15
·期 9
·1275
·
IF 4.8
Yarong Zhang,Hongyan Ma,Zhenyao Gao,Miao Li,Fan Yang,Lingbo Sun,Yuecheng Zhang
Apurinic/apyrimidinic endonuclease 1 (APE1) selectively cleaves the apurinic/apyrimidinic site (AP site) in DNA, playing a critical role in base excision repair and genomic stability maintenance. Aberrant APE1 expression has been linked to numerous diseases, including cardiovascular disorders, neurological conditions, and various cancers. However, existing methods for detecting trace levels of APE1 remain suboptimal for certain applications. To address this limitation, we developed an innovative biosensing platform for ultrasensitive APE1 detection by integrating APE1-specific site recognition with hybridization chain reaction (HCR)-based signal amplification, enabling enzyme- and label-free bioassays. In this system, APE1 recognizes and cleaves the AP site-containing hairpin (HP) probe, releasing a single-stranded HCR initiator that triggers cascaded HCR amplification. Owing to the high efficiency of HCR, this method achieves ultrahigh sensitivity, with a calculated detection limit of 1.0 × 10−8U/mL. Furthermore, the biosensor demonstrates robust performance in cell lysates and is applicable for screening and evaluating APE1 inhibitors.
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PubMed
高影响因子
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