JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
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2019
·Vol. 94
·期 8
·2512-2521
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IF 2.659
Qi Jiang,Runliang Zhu,Yanping Zhu,Qingze Chen
Background Developing effective removal processes for antibiotics has attracted increasing interest recently. In this work, a novel strategy involving the combination of photocatalysis with Fenton reaction using ferrihydrite/TiO 2 (Fh/TiO 2 ) nanohybrids was developed to efficiently degrade the antibiotic cefotaxime. Fh/TiO 2 nanohybrids were synthesized by simply growing Fh on the surface of commercially available TiO 2 . We expected that Fh of Fh/TiO 2 could capture photo-generated electrons from TiO 2 , inhibiting the recombination of electron–hole pairs; also, by virtue of Fe(III)/Fe(II) cycle on Fh/TiO 2 , photo-generated electrons could be continually transferred to H 2 O 2 to produce ·OH. Accordingly, high degradation efficiency of cefotaxime could be achieved. Results With UV light and H 2 O 2 , Fh/TiO 2 with a Fe/Ti molar radio of 7% showed high catalytic activity indeed, and its degradation rate for cefotaxime was nearly three times higher than that of TiO 2 . The decomposition of H 2 O 2 and production of ·OH in the UV+7%Fh/TiO 2 +H 2 O 2 system were also increased markedly. The large amount of Fe(II) on 7%Fh/TiO 2 determined in this system supported our hypothesis that Fh of 7%Fh/TiO 2 could capture photo-generated electrons from TiO 2 . Although dissolved iron was observed, the contribution of Fenton reaction by dissolved iron was rather limited. After four consecutive cycles, 7%Fh/TiO 2 still retained good stability. Conclusions The UV+7%Fh/TiO 2 +H 2 O 2 system provides a potential alternative in degradation of cefotaxime for further practical application, and it has the following advantages: high catalytic activity, simple preparation method, good stability and low cost, as well as continued catalytic activity after total consumption of H 2 O 2 . © 2019 Society of Chemical Industry
研究领域:
环境科学
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化学工程
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DOI
高影响因子
Microorganisms
·
2024
·Vol. 12
·期 4
·675
·
IF 4.1
Huizhong Pang,Kaixuan Zheng,Wenbo Wang,Mingjuan Zheng,Yudan Liu,Hong Yin,Daochuan Zhang
Cefotaxime (CTX) is an easily detectable antibiotic pollutant in the water environment, but little is known about its toxic effects on aquatic invertebrates, especially on the intestine. Here, we determined the oxidative stress conditions ofA. sinicaunder CTX exposure with five concentrations (0, 0.001, 0.01, 0.1 and 1 mg/L) for 14 days. After that, we focused on changes in intestinal tissue morphology and gut microbiota inA. sinicacaused by CTX exposure at 0.01 mg/L. We found malondialdehyde (MDA) was elevated in CTX treatment groups, suggesting the obvious antibiotic-induced oxidative stress. We also found CTX exposure at 0.01 mg/L decreased the villus height and muscularis thickness in gut tissue. The 16S rRNA gene analysis indicated that CTX exposure reshaped the gut microbiota diversity and community composition. Proteobacteria, Actinobacteriota and Bacteroidota were the most widely represented phyla inA. sinicagut. The exposure to CTX led to the absence of Verrucomicrobia in dominant phyla and an increase in Bacteroidota abundance. At the genus level, eleven genera with an abundance greater than 0.1% exhibited statistically significant differences among groups. Furthermore, changes in gut microbiota composition were accompanied by modifications in gut microbiota functions, with an up-regulation in amino acid and drug metabolism functions and a down-regulation in xenobiotic biodegradation and lipid metabolism-related functions under CTX exposure. Overall, our study enhances our understanding of the intestinal damage and microbiota disorder caused by the cefotaxime pollutant in aquatic invertebrates, which would provide guidance for healthy aquaculture.
研究领域:
环境科学
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微生物学
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毒理学
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DOI
PubMed
高影响因子
Scientific Reports
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2019
·Vol. 9
·期 1
·1-6
·
IF 4.011
Khan M. Z. H.,Daizy M.,Tarafder C.,Liu X.
In this work, we have successfully synthesized core-shell structured Au-PDA@SiO 2 nanospheres and decorated on reduced graphene oxide (rGO) modified glassy carbon electrode for the electrochemical detection of cefotaxime. The one-pot hydrothermal method was used to synthesis core-shell nanostructures by loading Au nanoparticles on polydopamine (PDA) coated SiO 2 nanospheres. The as-prepared Au-PDA@SiO 2 nanospheres were used to fabricate electrochemically reduced graphene oxide (rGO) modified glassy carbon electrode (Au-PDA@SiO 2 /rGO/GCE) for electrochemical determination of cefotaxime. Scanning electron microscopy, powder x-ray diffraction, transmission electron microscopy, and Fourier-transform infrared spectroscopy were used to confirm the structure and morphology of the as-prepared nanospheres. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were performed for electrochemical characterizations different modified electrodes. It was revealed that the nanocomposite modified electrodes exhibited excellent electrochemical performances for electrooxidation of target analytes and could achieve ultra-sensitive detections. A linear relationship was observed between peak currents and concentrations in the ranges of 1.0 × 10 −9 to 5.0 × 10 −8 M ( R 2 = 0.9877), and 1.0 × 10 −7 to 5.0 × 10 −6 M ( R 2 = 0.9821) for cefotaxime with a detection limit (S/N = 3) of 1.0 × 10 −10 M. It can be deduced that the proposed sensor is suitable for the sensitive detection of cefotaxime in pharmaceutical samples.
研究领域:
分析化学
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电化学传感器
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材料科学
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DOI
高影响因子
ACS Applied Materials & Interfaces
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2025
·
IF 8.2
Zhengjie Meng,Haixiao Huang,Jiaqi Guo,Dong Wang,Xinyue Tao,Qihao Dai,Yunhao Bai,Chenyu Ma,Luming Huang,Yangkai Fu,Chenyu Lu,Hengjian Wang,Qiyue Wang,Xueming Li,Hao Ren
Sepsis is a life-threatening organ dysfunction syndrome caused by a dysregulated host immune response to pathogenic infection. Due to its high mortality rate, it has been a major global public health problem. Recent studies have shown that the formation of immunothrombosis plays as a “double-edged sword” in the pathogenesis of sepsis, and how to properly regulate immunothrombosis to avoid organ damage and end the high-inflammation state as early as possible are the key steps for sepsis therapy. Considering the complexity of sepsis therapy, the development of an effective combined therapeutic strategy is the goal of this study. First, the insoluble Panexin1 (Panx1) channel inhibitor probenecid (Prob) was prepared as nanocrystals and administered via intramuscular injection. At the same time, septic mice were intravenously injected with cefotaxime sodium through the tail vein for combination therapy. After treatment, the number of infection foci and the level of serum inflammatory factors in septic mice were significantly reduced, and also neutrophil NETosis was significantly inhibited; thus, the survival rate of septic mice was dramatically increased. Pathological analysis revealed that the combination treatment was safe and effective and could significantly reduce the formation of immunothrombosis in septic mice.
研究领域:
医学
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免疫学
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药理学
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
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2025
·
IF 2.5
Yun Liu,Xuefang Shang,Ashraf H. Abadi,Mingpan Cheng
To address the challenge of antibiotic resistance and enhance the functionality of pharmaceutical materials, aldehyde chitosan-based dynamic polymers (ACs) incorporating amikacin and cefotaxime, denoted as ACs-amikacin and ACs-cefotaxime, respectively, were synthesized. These polymer's chemical structure was characterized using 1H-NMR and FT-IR analysis. Subsequently, methylthiazolyldiphenyl-tetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays were employed to evaluate their safety. Furthermore, it was demonstrated that these polymers retained the antibacterial activity of the antibiotics. This study introduces a novel strategy for dynamic natural materials capable of exerting a synergistic effect in pharmaceutical applications through the conjugation of natural materials with active pharmaceutical agents.
研究领域:
药用材料
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高分子化学
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抗菌研究
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药物递送系统
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产品关联
DOI
高影响因子
PROCESS BIOCHEMISTRY
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2021
·
IF 3.757
Yonghua Hu,Yuan Zhou,Xiaohua Hu,Qiaoling Chen,Yan Shi,Jiangxing Zhuang,Qin Wang
Cefotaxime sodium (CTX) was found to inhibit the activity of mushroom tyrosinase. The present study was conducted to investigate its inhibition of melanogenesis in B16F10 cells. CTX was found to inhibit melanin synthesis in B16F10 cells at a concentrations less than 200 μmol/L with no cytotoxicity. CTX reduced tyrosinase activity in a dose-dependent manner. CTX inhibited melanin formation from the results of the Masson-Fontana melanin staining and transmission electron microscope CTX influenced melanin production by influencing the expression of the proteins MITF, TRP-1, TRP-2 and TYR. Furthermore, CTX could down-regulate the intracellular cAMP/PKA/CREB contents, affecting the expression of MITF, and further down-regulate the expression of TRP-1, TRP-2 and TYR to inhibit melanin. Furthermore, CTX has been shown to inhibit body pigmentation in zebrafish. These results may provide a theoretical foundation for the new application of CTX in the field of medicine.
研究领域:
药理学
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细胞生物学
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分子生物学
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DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2020
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IF 7.183
Fan Yang,Guoyu Ding,Jin Wang,Zihan Liang,Boru Gao,Mengmeng Dou,Ce Xu,Shuming Li
Mesoporous graphitic carbon nitride (MCN) photocatalyst was blended in polyvinylidene fluoride (PVDF) membrane through immersion-precipitation phase transformation. Various techniques were adopted to characterize the structure and morphology of MCN-PVDF hybrid membrane, which indicated that MCN was successfully integrated in PVDF. The MCN 80 -PVDF membrane exhibited enhanced hydrophilicity and antifouling properties. As the amount of MCN addition increased, the contact angle of membranes decreased from 68.33° to 57.12°, whereas the flux recovery rate increased by 28% as compared to the pristine PVDF. Meanwhile, MCN-PVDF membrane possessed photocatalytic, self-cleaning and antimicrobial activities. The MCN 80 -PVDF membrane was used to degrade cefotaxime (CFX) under sunlight irradiation. After five cycles of experiments, the degradation rate of CFX remained 97.4%. Additionally, MCN 80 -PVDF membrane achieved 3 log of E. coli inactivation under visible light irradiation for 4 h. The photogenerated h + and reactive oxidative species were the primary reason for organics’ degradation and bacterial inactivation. In summary, the prepared hybrid MCN 80 -PVDF membrane with sunlight irradiation exhibited great potential for the treatment of real wastewater .
研究领域:
材料科学
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环境工程
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化学工程
·
水处理
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DOI
高影响因子
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