FOOD CHEMISTRY
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2021
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IF 7.514
Chen He,Jun Cao,Yuyan Bao,Zexin Sun,Zhongyuan Liu,Chuan Li
A lipidomic evaluation was performed on the tilapia muscle, head and viscera, including studying the composition, distribution and stereospecific characteristics of fatty acids and lipid species. The head and viscera lipids were significantly richer than the muscle lipids. Triacylglycerols were the predominant fraction (over 80% of total lipid in the muscle and head). Additionally, polyunsaturated fatty acids had higher percentages in phospholipids (30.35–52.05% of total fatty acids) than in triacylglycerols (18.11–25.15%). The C52:2 and C52:3 were the most abundant triacylglycerols, which indicates the potential application in infant food. Moreover, 622, 530 and 513 lipids were identified using ultraperformance liquid chromatography-quadrupole time-of-flight-mass spectrometry in the muscle, head and viscera, respectively. The three tilapia parts were distinguished using multivariate analysis. Five fatty acids and 33 lipid species were considered as the potential biomarkers. This comprehensive analysis will help to evaluate the lipid nutritional values and facilitate exploitation in tilapia consumption and processing.
研究领域:
营养学
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生物化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2021
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IF 7.514
Wensheng Yao,Yingxuan Cai,Dengyong Liu,Yu Chen,Jianrong Li,Mingcheng Zhang,Na Chen,Hao Zhang
In order to help the poultry industry to generate higher quality products, the headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) technique was used to identify volatile substances formed during the processing of Dezhou braised chicken (DBC). A total of 37 volatile substances including aldehydes, alcohols, ketones, esters, terpenoids, furans and pyrazines were identified during DBC processing across seven sampling stages. The analyses identified 2-ethylhexanol as a key flavor chemical within the chicken carcasses, and found that ethyl acetate, 1-hexanol, 4-methyl-2-pentanone and 1-pentanol were mainly produced during the deep-frying stage of processing. Stewing with herbs and spices was found to be an important stage in the flavor impartation process. 2-Butanone, n -nonanal, heptanal and ethanol were positively related to processing stage 3, whereas processing stage 4 was characterized by ethyl propanoate, benzaldehyde, butyl acetate, 2-pentyl furan and 2-heptanone. The processing stages 5, 6 and 7 were not significantly different (P > 0.05) from each other.
研究领域:
食品科学
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分析化学
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DOI
PubMed
高影响因子
Viruses-Basel
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2022
·Vol. 14
·期 11
·2431
·
IF 5.818
Jing Zheng,Wenyuan Chen,Hao Xiao,Fan Yang,Xiaowu Li,Jingdong Song,Lingpeng Cheng,Hongrong Liu
GP4, a newRalstonia solanacearumphage, is a short-tailed phage. Few structures ofRalstonia solanacearumphages have been resolved to near-atomic resolution until now. Here, we present a 3.7 Å resolution structure of the GP4 head by cryo-electron microscopy (cryo-EM). The GP4 head contains 540 copies of major capsid protein (MCP) gp2 and 540 copies of cement protein (CP) gp1 arranged in an icosahedral shell with a triangulation number T = 9. The structures of gp2 and gp1 show a canonical HK97-like fold and an Ig-like fold, respectively. The trimeric CPs stick on the surface of the head along the quasi-threefold axis of the icosahedron generating a sandwiched three-layer electrostatic complementary potential, thereby enhancing the head stability. The assembly pattern of the GP4 head provides a platform for the further exploration of the interaction betweenRalstonia solanacearumand corresponding phages.Keywords:Ralstonia solanacearumphage;icosahedral head;triangulation number T = 9;trimeric CP;Cryo-EM
研究领域:
病毒学
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结构生物学
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微生物学
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DOI
PubMed
高影响因子
CURRENT BIOLOGY
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2025
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IF 7.5
Junyu Zhao,Baishun An,Ning Cheng,Qiqi Dong,Cheng Wang,Xiaojing Chen
Reference frame is an essential concept for spatial navigation. The principles underlying the choice of reference frames by neuronal ensembles, especially under natural conditions, which involve sloping terrains, remain unclear. The posterior parietal cortex (PPC) is a central region for navigation and movement control, and it contains several distinct types of reference frames. Here, we investigated the influence of gravity signals on egocentric tuning and allocentric head direction tuning in the mouse PPC by introducing a mismatch between visual input and gravitational signals by tilting the ground. We found that the allocentric head direction tuning and egocentric boundary bearing tuning were differentially controlled by altered gravity signals. Specifically, the allocentric head direction signals became stronger, whereas the egocentric boundary signal was weakened. However, across horizontal and tilted conditions, the head direction tuning was less consistent, as the head directional signal could be anchored to a visual or gravity frame of reference. As the mismatch between gravitational and visual inputs increased, the gravity-anchored head direction cells became dominant. Notably, individual head direction cells can switch between reference frames. Together, these results suggest that PPC neurons exhibit a highly flexible selection of reference frames based on task demands, which may allow efficient navigation strategies in natural environments.
研究领域:
神经科学
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认知神经生物学
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空间行为学
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感觉整合
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运动控制
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DOI
PubMed
高影响因子
CHEMOSPHERE
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2023
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IF 8.8
Jun Zhang,Wenxing Yang,Zhuo Li,Feijun Huang,Kui Zhang
Cadmium is a naturally existing heavy metal and a notorious environmental pollutant. While its toxic outcomes and underlying mechanisms remain largely elusive. To explore the behavioral change caused by multigenerational exposure of cadmium to C. elegans , we challenged the C. elegans with cadmium for six generations and observed its impact on animal behaviors. Wild-type worms were randomly divided into two groups, the control and cadmium exposure groups. Locomotive and chemotactic behaviors were observed across six generations. Head thrashing frequency, chemotaxis index, and fold change index were used to evaluate the neurotoxicity of multigenerational cadmium exposure. Multigenerational cadmium exposure can trans -generationally increase the head thrashing frequency of C. elegans during swimming, and impair the chemotactic behaviors to isoamyl alcohol, diacetyl, and 2-nonanone. Our findings proposed a trans -generationally behavioral impact induced by multigenerational cadmium exposure.
研究领域:
毒理学
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环境科学
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神经科学
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发育生物学
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DOI
PubMed
高影响因子
STAR Protocols
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2024
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IF 1.3
Siyao Zhou,Shumin Duan,Hongbin Yang
Summary To exclude the influence of motion on in vivo calcium imaging, animals usually need to be fixed. However, the whole-body restraint can cause stress in animals, affecting experimental results. In addition, some brain regions are prone to bleeding during surgery, which lowers the success rate of calcium imaging. Here, we present a protocol for calcium imaging using heparin-treated fiber in head-fixed mice. We describe steps for stereotaxic surgery, including virus injection and optic fiber implantation, fiber photometry, and data analysis. For complete details on the use and execution of this protocol, please refer to Du et al. 1
研究领域:
神经科学
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生物医学工程
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成像技术
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DOI
PubMed
高影响因子
BIOORGANIC & MEDICINAL CHEMISTRY
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2025
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IF 3
Chao Liu,Yuhao Jiang,Wenliang Xue,Jinyu Liu,Zihao Wang,Xinsong Li
Lipid nanoparticles (LNP) are recognized as the most efficient non-viral carriers for the delivery of nucleic acids including small interfering RNA (siRNA) and messenger RNA (mRNA). Ionizable lipid within the system is pivotal component influencing encapsulation, endosomal escape, delivery efficiency and immunogenicity. Accordingly, the precision design of ionizable lipids is a key step in the development of LNP. In this report, we constructed sixteen Janus dendritic ionizable lipids by varying numbers and alkyl chain length of tails based on different ionizable head containing hydroxyl and tertiary amine groups. The corresponding LNP were prepared by using microfluidic mixing device, with all samples exhibiting particle size around 100 nm and polydispersity index (PDI) below 0.2. In vivo validation demonstrates that two optimized ionizable lipids containing two hydroxy groups, two tertiary amines and six hydrophobic chain tails (U-502, U-503) show superior delivery efficiency compared to lipids with less tails and commercial ALC-0315. Hematoxylin and Eosin (H&E) staining of tissues, immunogenicity, liver and kidney function tests additionally confirm that both ionizable lipids have favorable biocompatibility and low in vivo toxicity. Lysosomal escape and cell transfection data verify the in vitro delivery efficacy of these LNP. Taken together, Janus dendritic lipids with fine designed ionizable head and multiple hydrophobic tails have improved mRNA delivery efficiency and biosafety, which may be promise in the development of delivery system.
研究领域:
药剂学
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分子生物学
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纳米技术
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药物递送系统
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
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2021
·Vol. 9
·期 12
·2877-2884
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IF 6.331
Yanping Cao,Shengjie Liu,Zhaoqiang Wu,Hong Chen
Linear poly(N-hydroxyethylacrylamide) (PHEAA) is regarded as one of the most promising antifouling materials because of its excellent antifouling properties and good hemocompatibility. However, the antifouling performance of topological PHEAAs remains largely unknown. Herein, the preparation of antifouling surfaces based on a tadpole-shaped PHEAA coating is reported for the first time, and how the tadpole-shaped PHEAA architecture affects antifouling performance is investigated. It is shown that the tadpole-shaped PHEAA-modified surfaces exhibit better antifouling performance than linear copolymer precursor-modified surfaces with identical molar masses and chemical compositions. This may be primarily attributed to the presence of cyclic PHEAA head chain segments in the tadpole-shaped PHEAA copolymer, and the absence of interchain entanglements can facilitate the formation of smoother and densely packed grafts, which result in better antifouling properties.
研究领域:
材料科学
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表面化学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
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2021
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IF 9.028
Yuxuan Liu,Yonghua Wang,Xin Ling,Zhenhua Yan,Donghai Wu,Jianchao Liu,Guanghua Lu
Nanoplastics with small particle sizes and high surface area/volume ratios easily absorb environmental pollutants and affect their bioavailability. In this study, polystyrene nanoplastic beads (PS-NPBs) with a particle size of 100 nm and butyl methoxydibenzoylmethane (BMDBM) sunscreen in personal-care products were chosen as target pollutants to study their developmental toxicity and interactive effects on zebrafish embryos. The exposure period was set from 2 to 12 h postfertilization (hpf). BMDBM and PS-NPBs significantly upregulated genes related to antioxidant enzymes and downregulated the gene expression of aromatase and DNA methyltransferases, but the influenced genes were not exactly the same. The combined exposure reduced the adverse effects on the expression of all genes. With the help of the single-cell RNA sequencing technology, neural mid cells were identified as the target cells of both pollutants, and brain development, head development, and the notch signaling pathway were the functions they commonly altered. The key genes and functions that are specifically affected by BMDBM and/or PS-NPBs were identified. BMDBM mainly affects the differentiation and fate of neurons in the central nervous system through the regulation of her5, her6, her11, lfng, pax2a, and fgfr4. The PS-NPBs regulate the expression of olig2, foxg1a, fzd8b, six3a, rx1, lhx2b, nkx2.1a, and sfrp5 to alter nervous system development, retinal development, and stem cell differentiation. The phenotypic responses of zebrafish larvae at 120 hpf were tested, and significant inhibition of locomotor activity was found, indicating that early effects on the central nervous system would have a sustained impact on the behavior of zebrafish.
研究领域:
毒理学
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发育生物学
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分子生物学
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环境科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）