JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2024
·
IF 3.1
Huaqing Sun,Jian Li,Liang Li,Chunyu Wang,Xiujuan Liu,Ming Shi,Zhiwei Liu,Kun Li
Lung cancer is the most common malignancy worldwide. Early diagnosis helps to reduce mortality and improve survival. Aptamers are widely used in cancer screening because of their high specificity, good stability and low cost. In this study, using the specific aptamer of lung cancer serum, the sandwich method colloidal gold test strip was prepared by isothermal amplification technique and the principle of nucleic acid hybridisation for the early diagnosis of lung cancer. The results showed that the test strip was positive in 8 patients with lung cancer, which was consistent with the actual cases. The test strip can accurately identify lung cancer patients. The concentration range of nucleic acid detection is 1 × 10 −4 - 7 × 10 −4 mol/L, and the detection limit is 0.67 mM. The test strip detection method has low cost and simple operation, and provides a reference for the development of home portable tumor early detection.
研究领域:
肿瘤学
·
分子生物学
·
生物技术
·
诊断技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Sensors and Actuators Reports
·
2024
·
IF 6.5
Song Ge,Mingxin Wang,Shunhua Zhu,Han Wu,Jing Li,Dongsheng Liu,Qingli Huang,Shibao Li,Xiaojing Sun
Purpose As an important reference index for the early diagnosis of non-small cell lung cancer (NSCLC), CYFRA21-1 still lacks the detection of low equipment cost, wide linear range and high sensitivity. Surface-enhanced Raman scattering (SERS) is a vibration spectrum technology based on the surface plasma of precious metal nanoparticles, which has been effectively applied to the detection of tumor markers. The combination of SERS technology and sensors has great potential in the ultrasensitive detection of tumor markers. The purpose of this study was to develop a sensitive method using SERS to quantitatively detect CYFRA21-1 for early diagnosis of NSCLC. Methods A double-antibody sandwich immunoassay based on SERS was designed and tested to implement the ultrasensitive detection of CYFRA21-1 in the serum of NSCLC patients. Results Gold @Ag nanorods (Au @Ag NRs) with higher Raman signals were prepared and used as probes, while magnetic graphene oxide was used as a magnetic substrate. The immunized probe, immune substrate and CYFRA21-1 standard substance in the buffer system formed a double-antibody sandwich structure. The standard curve displayed a liner range from 1pg mL −1 to 10 ng mL −1 , and the detection limit (LOD) is 0.8943pg mL −1 . The Raman intensity exhibited a wide linear relationship with the logarithm of CYFRA21-1 concentration. Conclusion Our study successfully established a double-antibody sandwich immunoassay based on SERS. This method demonstrated high specificity and sensitivity for detecting CYFRA21-1 protein content in serum. It has the potential to be applied for early detection of lung cancer biomarkers.
研究领域:
肿瘤学
·
医学诊断
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Yuhan Zhu,Zuwei Xu,Jie Gao,Weihao Ji,Jingdong Zhang
The progesterone (P4) level in body fluids can act as an indicator for early pregnancy diagnosis and offers insight into mammalian somatic function. In this work, we designed an antibody-aptamer based sandwich assay as a cathodic photoelectrochemical (PEC) biosensor for P4 detection. The composites of carbon dots and graphene oxide (CDs-GO) with favorable cathodic photocurrent response were used as photoactive materials on which the antibody (Ab) of P4 was immobilized. Meanwhile, high affinity truncated P4 aptamer was immobilized on Au–CuO–Cu 2 O to act as a bioconjugate . When P4 was present, the aptamer-Au-CuO-Cu 2 O bioconjugate could amplify the cathodic photocurrent of CDs-GO modified electrode through Ab-P4-aptamer interactions. Under optimum conditions, the cathodic photocurrent of the constructed PEC biosensor was found to increase linearly with P4 in a wide concentration range from 0.5 nM to 180 nM, with a low detection limit (3S/N) of 0.17 nΜ. The proposed cathodic PEC sensing platform demonstrated high selectivity , satisfying reproducibility, good stability. The sensor was successfully applied in the determination of P4 in human serum samples.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Xue-cheng Sun,Zhen Yang,Chong Lei,Yan Liu,Lei Guo,Yong Zhou
A novel bio-sensing system based on a micro-fluxgate and use of magnetic beads labels was developed for detection of Escherichia coli O157H:H7. The micro fluxgate sensor was fabricated using standard microfabrication processes. Magnetic core of the sensor was made using Fe-based amorphous ribbon core and a 3-D magnetic sensitive element was made of Cu solenoid coils. Response of the micro-fluxgate-based bio-sensing system to superparamagnetic Dynabead was first evaluated with different concentrations of Dynabeads. It was found that a concentration as low as 10 ng/ml could be detected with an external dc magnetic field ranging from 450 μT to 700 μT. Double antibody sandwich assay was employed in preparation of E. coli O157H:H7 samples. Detection of E. coli O157H:H7 antigens at different concentrations were demonstrated. A minimum detectable concentration of 100 CFU/ml was observed for this bio-sensing system. On account of the detection performance and other advantages such as miniaturization, portability and stability, this bio-sensing system is a potential candidate for rapid analysis E. coli O157:H7 and other pathogenic bacteria . It is also of considerable interest for biomedical applications employing known specific binding of target and labels.
研究领域:
微生物学
·
生物医学工程
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2018
·
IF 2.746
Sizhu Tian,Jia Jiang,Shuang Zang,Kun Wang,Yong Yu,Xuwen Li,Hanqi Zhang,Aimin Yu,Ziwei Zhang
A novel electron spin resonance (ESR) spectroscopy method for determination of rabbit IgG was presented. Anti-rabbit IgG antibody was bound to Fe 3 O 4 nanoparticles (FeNPs). The FeNPs were used as probe to give ESR signal . After the optimization of the assay, a molar ratio 500:1 of antibody to FeNPs was selected. The sepharose beads were used as solid phase and used to separate the analyte from the sample solution. Two typical immunoassay formats, sandwich and competitive formats, were applied to the determination of IgG. The analytical sensitivities obtained by sandwich and competitive formats were 0.13 μg mL −1 and 0.56 μg mL −1 , respectively. The two formats were applied to the determination of the rabbit IgG in rabbit serum. The diluted sample was directly analyzed and other additional sample treatment was not required. The analytical results of the spiked samples indicated that the recoveries of the analytes were acceptable. Furthermore, the sample preparation time used in this method is shorter than most existing immunoassay methods .
研究领域:
分析化学
·
免疫学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Journal of Analytical Methods in Chemistry
·
2022
·
IF 2.594
Yanling Zhao,Yanfei Wen,Xing Hu,Bing Zhang
We proposed a colorimetric immunosensor based on g-C3N4@Fe3O4 nanocomposite-mediated transformation strategy for sensitive detection of carcinoembryonic antigen (CEA). The g-C3N4@Fe3O4 nanocomposite was synthesized and characterized by the scanning electron microscope (SEM), energy dispersive X-ray spectra (EDX), X-ray powder diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). Fe3+ derived from g-C3N4@Fe3O4 nanocomposite could combine with sodium salicylate to form purple complex products. Based on this color development, the sandwich colorimetric immunoassay was built by utilizing g-C3N4@Fe3O4 nanocomposite as nanolabels on the microplate. With the increasement of CEA concentration, the purple color showed a gradient change. Under optimal conditions, the linearity range is 0.001–50 ng/mL with the detection limit of 0.35 pg/mL for CEA. More importantly, the colorimetric immunoassay has good selectivity, specificity, repeatability, and stability.
研究领域:
生物传感器
·
分析化学
·
癌症诊断
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2015
·Vol. 182
·期 15
·2411-2417
·
IF 3.741
Yang Zhen,Liu Yan,Lei Chong,Sun Xue-cheng,Zhou Yong
We report on a method for the determination of magnetic bead-labeled C-reactive protein (CRP), a biomarker of cardiovascular diseases and inflammations. It is using a flexible giant magnetoimpedance (GMI)-based platform. Micro-patterned GMI sensing elements were prepared from a cobalt-based commercial amorphous ribbon (Metglas® 2714A) using micro electro-mechanical system (MEMS) technology. A gold film was then deposited on the GMI sensing element to act as a support for the immuno platform. Sandwich assays are performed using antibody-antigen combinations and biotin-streptavidin interactions on the gold film substrate surface via self-assembled layers. The GMI ratios of the sensors with different concentrations of antigen against CRP were investigated. The results show that the presence of CRP antigens on the biosensor improves the GMI effect owing to the induced magnetic dipole of superparamagnetic beads, and that the GMI ratios show distinct changes at high frequency. This bioassay for CRP has a linear detection range between 1 to 10 ng·mL −1 . This new method in our perception provides a widely applicable basis for rapid diagnostic testing and will pave the way for future development of electrochemical point-of-care diagnostic devices for cardiac diseases.
(a) Graphical illustration of CRP test setup. (b) Magnetic field arrangement of the beads under an applied magnetic field. (c) GMI changes in relation to the concentration of the CRP
研究领域:
生物医学工程
·
材料科学
·
医学诊断
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Journal of Biomedical Nanotechnology
·
2022
·Vol. 18
·期 11
·2484-2497
·
IF 3.641
Li Guiyin,Chen Min,Li Haimei,Li Xinhao,Yao Xiaoqing,Li Liuxun
Golgi protein 73 (GP73) is a novel biomarker for the detection of hepatocellular carcinoma. We prepare a colorimetric aptasensor for GP73 detection based on the catalytic performance of the H-rGO-Mn3O4 nanozyme. The H-rGO-Mn3O4 nanozyme serves
as a peroxidase mimetic which can enhance the reaction with the substrate (3, 3,’ 5, 5′-tetramethylbenzidine, TMB) and H2O2. The peroxidase-like activity of H-rGO-Mn3O4 features a 1.78-fold higher binding affinity value (Km
)
for TMB compared with that of horseradish peroxidase. The H-rGO-Mn3O4 nanozyme not only exhibits admirable peroxidase-like activities due to the synergistic effect of Mn3O4 NPs, rGO and hemin, but also has a large specific surface area to endow the
GP73 aptamer with specific recognition capabilities. A sandwich colorimetric aptasensor is formed to realize the visual detection of GP73 through catalyzing the H2O2-mediated oxidation peroxidase substrate TMB to oxidized TMB accompanied by a color change from colorless
to blue. Under optimal conditions, the logarithm of the GP73 concentration (0.05–50.0 ng/mL) shows a good linear relationship with the absorbance. The calibration equation is Y =−0.0383 lgC+0.4835, with R2 of 0.9964, and the lowest limit of detection is 36.94
pg/mL. Additionally, the content of GP73 in human serum samples is directly detected, and the relative standard deviation is 0.49–4.91%. Compared with the enzyme-linked immunosorbent assay method, the relative error is 0.23–3.61%. All in all, colorimetric aptasensor is demonstrated
to exhibit excellent specificity, stability, and reproducibility.
研究领域:
生物标志物研究
·
纳米技术
·
分析化学
·
癌症诊断
·
生物化学
✓ 已验证
DOI
高影响因子
Science Advances
·
2025
·Vol. 11
·期 29
·
IF 12.5
Shengbo Chen,Ziyu Guo,Boxi Lu,Mengsi Sun,Saijie Wang,Shunhang Li,Yizhou Jiang,Qiwu Wei,Dou Wang,Xingyu Jiang
C-peptide is a critical biomarker for evaluating pancreatic β cell function and managing diabetes. However, conventional methods such as enzyme-linked immunosorbent assay are limited by lengthy processing times and the necessity for invasive sample collection. This study introduces a regenerable, portable, and wearable device for minimally invasive, real-time monitoring of C-peptide in dermal interstitial fluid (ISF). The device uses a microneedle array and negative pressure extraction for ISF collection. A double-antibody sandwich approach was used to detect C-peptide, and an antibody regeneration strategy was applied to remove bound antigen-antibody complexes, thereby achieving repeatable detection, enabling at least 10 detection assays on the same chip with negligible signal attenuation. The compact design features a skin contact area of only 3 cm by 5.4 cm and excellent biocompatibility. Integrated with a mobile app, the device provides real-time concentration reading. Mouse and rabbit tests confirm effective ISF sampling and monitoring accuracy, demonstrating its potential for at-home diabetes management and broader wearable biosensing applications.
研究领域:
生物医学工程
·
生物传感器
·
糖尿病研究
·
可穿戴设备技术
·
即时检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Yuqing Zhang,Gongwei Sun,Yi Zhang,Biao Huang,Zhi Xing,Sichun Zhang,Xinrong Zhang
Inductively Coupled Plasma Mass Spectrometry (ICP-MS) based immunoassay method has been proposed in multiple immunoassays but has not been used in competitive and sandwich formats immunoassay simultaneously. The two immunoassays were usually conducted separately in clinical field depending on the size and the amount of binding sites of targets. We proposed an immunoassay method based on magnetic beads and ICP-MS detection that could be suitable for both small and large molecules. The functionalized magnetic beads were added to capture the immune complex after immune reaction. In this way, thyrotropin and free thyroid hormone can be captured, separated and then detected according to the elemental tags by ICP-MS simultaneously. The new method was evaluated by comparing the results with time resolved fluorescence immunoassays (TRFIA). The dynamic ranges of TSH and FT 4 were 0.16–105 mU/L and 3.5–65 pmol/L, respectively. The limits of detections were 0.06 mU/L for TSH and 1.59 pmol/L for FT 4 . And the relative standard deviations (RSD) of TSH and FT 4 were 4.64% at 2.5 mU/L and 1.87% at 5.85 pmol/L. This immunoassay method enables the determination of small and large biomolecules simultaneously via competitive and sandwich immunoassay formats.
研究领域:
分析化学
·
临床化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Wei Li,Qi Zhang,Yijia Wang,Yanyan Ma,Zhanchen Guo,Zhen Liu
Molecularly imprinted polymers (MIPs) and aptamers, as effective mimics of antibodies, can overcome only some drawbacks of antibodies. Since they have their own advantages and disadvantages, the combination of MIPs with aptamers could be an ideal solution to produce hybrid alternatives with improved properties and desirable features. Although quite a few attempts have been made in this direction, a facile and controllable approach for the preparation of aptamer–MIP hybrids still remains lacking. Herein, we present a new approach for facile and controllable preparation of aptamer–MIP hybrids for high-specificity and high-affinity recognition toward proteins. An aptamer that can bind the glycoprotein alkaline phosphatase (ALP) with relative weak affinity and specificity was used as a ligand, and controllable oriented surface imprinting was carried out with an in-water self-polymerization system of dopamine. A thin layer of polydopamine was formed to cover the template to an appropriate thickness. After removing the template from the polymer, an aptamer–MIP hybrid with apparently improved affinity and specificity toward ALP was obtained, giving cross-reactivity of 3.2–5.6% and a dissociation constant of 1.5 nM. With this aptamer–MIP hybrid, a plasmonic immunosandwich assay (PISA) was developed. Reliable detection of ALP in human serum by the PISA was demonstrated.
研究领域:
生物材料
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2022
·
IF 5.999
Zhi Luo,Dongmiao Qin,Yusheng Wu,Shuo Meng,Weiming Mo,Biyang Deng
A sandwich electrochemiluminescence (ECL) immunosensor based on an N-(4-aminobutyl)-N-ethylisoluminol-graphene oxide-Ag nanoparticle (ABEI-GO-AgNPs) complex and cysteine silver nanowires (AgCysNWs) was prepared to detect prostate-specific antigen (PSA). Our results showed that an ECL signal probe, ABEI-GO-AgNPs, with an ultrahigh specific surface area, favorable catalytic properties, and electrical conductivity was prepared by a one-step synthesis method. ABEI-GO-AgNPs with good biocompatibility immobilized secondary antibody (Ab 2 ) via Ag N bonds. Furthermore, AgCysNWs containing many –COOH groups were prepared and used to enrich primary antibody (Ab 1 ), which could be used as an affinity probe for the selective capture of PSA. Lastly, through layer-by-layer assembly, we established an ECL immunosensing platform for the sensitive detection of PSA. Under the optimized conditions, the designed ECL immunosensor showed promising sensitivity and selectivity for the detection of PSA in the linear range of 5.5 × 10 −7 –5.5 ng/mL, with a detection limit of 1.2 × 10 −7 ng/mL. The constructed ECL sensing platform possessed good specificity, reproducibility, and stability and could detect PSA in actual human serum samples.
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）