WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
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2011
·Vol. 28
·期 3
·857-863
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IF 1.214
Ye Li,Zhang Chunyan,Li Jiyang,Shi Xunlong,Feng Meiqing
Calcium is a known signalling molecule in eukaryotic cells and plays a central role in the regulation of many cellular processes. In the following study, we report on the effect of external calcium treatments on the biotransformation of ginsenoside Rb1 to ginsenoside Rd by Paecilomyces bainier 229-7. We observed that the intracellular calcium content of P . bainier 229-7 mycelia was increased in response to exposure to high external Ca 2+ concentrations. Both ginsenoside Rd biotransformation and β-glucosidase activity were both found to be dependent on the external calcium concentration. At an optimal Ca 2+ concentration of 45 mM, maximal ginsenoside Rd bioconversion rate of 92.44% was observed and maximal β-glucosidase activity of 0.1778 U was reached in a 72-h biotransformation. The Ca 2+ channel blocker Verapamil blocked the trans-membrane influx of calcium and decreased ginsenoside Rd biotransformatiom. In addition, β-glucosidase activity and ginsenoside Rd content decreased by 36.0 and 29.2% respectively after a 72-h incubation in the presence of 0.05 mM Calmodulin (CaM) antagonist Perphenazine. These results suggest that both Ca 2+ channels and CaM are involved in ginsenoside Rd biotransformation via regulation of β-glucosidase activity. This is the first report regarding the effects of calcium signal transduction on biotransformation and enzyme activity in fungi.
研究领域:
药理学
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微生物学
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生物化学
✓ 已验证
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DOI
PubMed
高影响因子
Analytical Methods
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2025
·
IF 2.6
Xue Kong,Yan Liu,Jinghui Lyu,Zheng-Zhi Yin,Yi Zhang
Osteopontin (OPN) is an important biomarker for reflecting osteoarthritic inflammation and endochondral ossification. In the field of electroanalysis, OPN belongs to non-electroactive protein, which is usually detected by means of an outer probe or biolabel. Here, a novel microsensor can directly electroanalyze OPN, which was constructed by coordinating surface molecularly imprinted polymer (SMIP) with a built-in electroactive probe of poly (methylene blue) (pMB) on an acupuncture needle microelectrode (ANME). The OPN template can be reversibly anchored using 4-mercaptophenylboric acid (4-MBPA) via a borate bond between phenylboronic acid and the external cis-diol of the glycoprotein. Methylene blue (MB) and dopamine (DA) were sequentially electropolymerized and grown around templates, which played pivotal roles in the detection signal from the built-in pMB through the imprinted nanocavities. After the recombination of OPN molecules with imprinted nanocavities, the current strength of built-in pMB could be impeded with a highly sensitive response. This microsensor shows a linear relationship to the concentration of OPN from 0.01 to 1000 ng mL-1 with a detection limit of 3 pg mL-1. The microsensor also exhibits high selectivity and stability, which is attributed to the recognizing ability of the imprinted nanocavities and the hindrance and anti-interference function of coated polydopamine (pDA). This strategy of preparing a sensor shows practical and scientific significance for functionalizing microelectrodes and constructing microsensors for non-electroactive glycoproteins. In the future, it will be fascinating to integrate this microsensor with the acupuncture technique.
研究领域:
生物医学工程
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分析化学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Weiyi Lu,Bin Yang,Zihua Guo,Zhen Tian,Wenhui He,Tiancheng Yang
Phenothiazine drugs are widely used to treat psychotic diseases in humans. The phenothiazine ligand enables unique interaction between the drugs and DNA double helix. We discovered that it was impossible to select aptamer to selectively bind with phenothiazine drug. In this study, an alternative fluorescence sensor array was first developed to accurately discriminate four typical phenothiazine drugs based on machine learning. As the eight sensor elements, versatile DNA probes were designed based on different oligonucleotides , two unique fluorescence dyes , and diverse buffer solutions. The fingerprint data can offer the fluorescence response patterns against each phenothiazine drug. Linear discriminant analysis (LDA) can discriminate single one or mixture samples of phenothiazine drugs with 100% accuracy. Besides, one neural network model showed a good linear relationship between predicted and actual concentrations to quantitatively assay specific phenothiazine drugs. The proposed fluorescence sensor array presents a new way to accurately discriminate phenothiazine drugs in pharmacy or biology field.
研究领域:
生物化学
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药学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Qiang Hou,Yali Wang,Jian Hu,Jianxiong Zhang,Caihua Zhang,Wang Song,Xuesong Wang,Baiqin Zheng,Xin Zhou
Due to their potential entry into the human body via the food chain, phenothiazine drugs and their metabolites pose specific health hazards, making their detection in edible tissues of paramount importance. In this research endeavor, a highly sensitive multi-residue analytical method has been devised for the detection of phenothiazine drugs and their metabolites in animal-derived food products, utilizing high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). The preparation of samples involved the use of 1% formic acid in acetonitrile as the extraction solvent following sample weighing, with multiple extraction steps diligently performed to ensure optimal outcomes. The sample, post-extraction, was directly introduced into the purification column, yielding significant time savings and rendering the process more environmentally sustainable. This procedural step effectively eliminated interfering substances from the sample matrix, including proteins, salts, and phospholipids. Analysis of the extracted samples was conducted through HPLC-MS/MS, employing an electrospray ionization (ESI) ion source. A comprehensive panel of 20 phenothiazine drugs and their metabolites was successfully resolved within a mere 10-min chromatographic run. A robust linear correlation (R 2 ≥ 0.99) was attained within a judiciously chosen range of concentrations. Method validation was meticulously conducted across multiple animal species, including trout, white shrimp, pork, beef and chicken. The developed analytical method has been effectively deployed for the screening and quantification of phenothiazine drugs in real-world samples sourced from commercial livestock and aquatic products.
研究领域:
食品安全
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分析化学
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毒理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Yuanhui Chong,Qiong Liu,Zeqi Wang,Liukai Zhu,Fengxin Guo,Yu Li,Tao Li,Baozeng Ren
Perphenazine has rather effective in the treatment of mental illness. In this work, the solid-liquid equilibrium data of perphenazine in eleven organic neat solvents were determined by laser dynamic method under the atmospheric pressure and temperature range of 278.15 K ∼ 318.15 K. The selected solvents were n-propanol, n-butyl alcohol, methyl acetate, ethyl acetate, n-propyl acetate, n-amyl acetate, N, N-dimethylformamide (DMF), N, N-dimethylacetamide (DMAC), acetone, 2-methoxyethanol, glycol ether, respectively. From the results, the solubility of perphenazine is all positively correlated with temperature. Separately, we have adopted the KAT-LSER model and molecular dynamics simulation to investigate the influence of solvent effects and solute-solvent interaction on solute solubility. Meanwhile, there were four activity coefficient models being used to correlate the solubility data and Average relative deviation of models are 4.13% (Margules model), 3.94% (NRTL model), 4.49% (NRTL-SAC model) and 3.06% (UNIQUAC model), respectively, all of which do not exceed 5%. It manifests that the four activity coefficient models can fit the experimental values well, in which the UNIQUAC model gives the best regression effect. Moreover, the apparent thermodynamic properties( △ sol G° , △ sol H° and △ sol S° ) of perphenazine in various solvents were calculated by Van’t Hoff equation. In accordance with the analysis and calculation of dissolution, the dissolution process is an endothermic entropy-driven process. This research can provide basic data for the crystallization process of perphenazine.
研究领域:
药学
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物理化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2023
·Vol. 58
·期 5
·2599-2608
·
IF 3.3
Cen Chen,Yuhang Zhang,Yulong Tang,Guangrong Huang,Han Jiang
Summary Magnetic molecularly imprinted polymers (MMIPs) were prepared by surface precipitation polymerisation, which used xylazine as its template molecule and Fe 3 O 4 as its support. The characterisation analysis and adsorption experiments of MMIPs were carried out. At the same time, promethazine, chlorpromazine, trifluoperazine, acepromazine and perphenazine were selected as structural analogues for selective experiments. The results showed MMIPs were successfully prepared with small particle size, excellent magnetic and thermal stability, high adsorption capacity and selectivity for xylazine. The maximum adsorption capacity of MMIPs for xylazine was 4.2017 mg g −1 , and the adsorption reached binding equilibrium within 30 min. The magnetic solid phase extraction (MSPE) based on MMIPs and traditional solid phase extraction (SPE) based on SPE column for xylazine in milk samples were compared and xylazine was analysed by HPLC. Although the difference between the two SPE methods was not significant, the MSPE method procedure took only about 35 min, which was faster than the traditional SPE method (>100 min), and the MSPE method had lower LOD (2.62 ng mL −1 ). MSPE has the advantages of simple operation, short extraction time, low cost and friendly to the environment, it has good application potential in the detection of xylazine in milk samples.
研究领域:
食品科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2023
·
IF 3.4
Huanhuan Yang,Cong Liu,Chuanhong Zhu,Yan Zheng,Juan Li,Qianyun Zhu,Hao Wang,Xiangming Fang,Quan Liu,Man Liang,Zilong Liu
Determination of antipsychotics from biological samples is meaningful in the field of clinical and forensic medicine. Compared to blood, keratinized matrices such as hair and nails have attracted increasing attention in drug analysis for wider detection window. Nevertheless, the distribution and stability of antipsychotics in keratinized matrices are not clarified yet. Therefore, we developed a LC-MS/MS based method for simultaneous determination of 10 antipsychotics from blood, hair and nails, with high recovery (78.1–107.4%, 71.3–93.5% and 75.2–90.5%), low LOD (5–10 pg/mL, 5–10 pg/mg and 5 pg/mg) and high accuracy (96.0%−101.6%, 97.5%−102.5% and 97.6–101.7%). The method was applied to sets of blood, hair, and nail samples of 54 patients who received long-term therapy, and significant correlations between drug concentrations in blood and hair was found, while the correlation between nails and other matrices varied depending on the drug. Except for olanzapine , the concentrations of antipsychotics in segmental hair samples were consistent with drug exposure. Besides, the hair and nail samples of suspects in two forensic cases were analyzed and provided supporting evidence for the suspects’ psychiatric state. Our research offered a deeper understanding of keratinized matrix as stable and retrospective bio-samples for antipsychotics detection in forensic practice.
研究领域:
临床医学
·
法医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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