Food Chemistry-X
·
2026
·
IF 9.2
Liru Ma,Zhen Feng,Chao Zhang,Haode Chang,Wenjing Zhang,Quanmiao Zhang,Yihong Bao,Chunhe Gu
This study evaluated polyphenol fractions from unripe (J1), underripe (J2), and ripe (J3) jackfruit to explore stage-dependent industrial potential. Free (FP), conjugated (CP), and bound phenolics (BP) were isolated and assessed for antioxidant and enzyme inhibitory activities. Ripening significantly altered composition and bioactivity. CP antioxidant capacity and inhibition of α-glucosidase, pancreatic lipase, and xanthine oxidase increased progressively from J1 to J3, while FP and BP showed fluctuating trends. Ripe fruit (J3) exhibited the strongest CP-related bioactivities and a more diverse conjugated phenolic profile, whereas early stages had greater FP compositional complexity. Molecular docking supported these findings, identifying cyclomorusin as a potent ligand for α-glucosidase (−9.6 kcal/mol) and xanthine oxidase (−11.8 kcal/mol), and cyclokievitone for pancreatic lipase (−6.7 kcal/mol). These results demonstrate stage-specific functional differentiation and support targeted industrial utilization for health promotion.
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Journal of Food Measurement and Characterization
·
2026
·
IF 3.6
Li Qing,Wang Ke,Chen Xiaoling,Hu Jinmei,He Xin,Zhou Lin
Given the global rise in obesity and metabolic syndrome, plant-based functional beverages that regulate glycemia and lipidemia have attracted increasing interest. This study focused on Millettia speciosa Champ. leaf Golden-flower fungus fermented tea (MLGFT), a novel fermented tea developed from underutilized leaf biomass. The brewing process was optimized via single-factor and response surface methodology, yielding optimal parameters of 85 °C, 7.3 min, and 146 mL of water, which maximized total phenolic content (124 µg/mL). The optimized tea infusion (OTI) exhibited potent in vitro enzyme inhibition: (93.45 ± 0.98) % against α-glucosidase, (45.52 ± 1.11) % against α-amylase, and (81.47 ± 0.90) % against pancreatic lipase. In cellular models, the freeze-dried extract (ME) dose-dependently enhanced glucose consumption in insulin-resistant HepG2 cells, with effects comparable to metformin at 100 mg/L. In a model of lipid accumulation, ME at 100 mg/L significantly improved the lipid profile, reducing TC, TG, and LDL-C by 63.02%, 43.50%, and 71.79%, respectively, while increasing HDL-C by 46.15%. UPLC-HRMS analysis revealed that the extract is rich in phenolics, particularly epicatechin gallate. These findings demonstrate that MLGFT exhibits notable dual hypoglycemic and hypolipidemic effects, supporting its use as a promising functional beverage for metabolic health management.
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Life-Basel
·
2026
·Vol. 16
·期 2
·235
·
IF 3.9
Qingfeng Guo,Xia Zhang,Hao Zhang,Mengxue Wang,Haoran Zhou,Meiru Chen,Zhenhua Yin,Juanjuan Zhang,Baocheng Yang,Li Wang,Lin Chen
Originating in East Asia, the adzuki bean (Vigna angularis) is a diploid crop mainly grown in this region that belongs to the Fabaceae family. As a potential functional food resource with both medicinal and nutritional value, it offers various health benefits. However, research on its chemical constituents, particularly key bioactive components such as triterpenoid saponins and flavonoids, remains relatively limited. In this study, a new triterpenoid saponin, adzukisaponin A (1), along with eleven known compounds (2–12), were isolated from adzuki bean (V. angularis). Among them, compounds3(yunganoside B1),6(3β-acetyl oleanolic acid), and7(β-amyrin) are reported from this source for the first time. Saponins1–3and flavonoid8exhibited significant dual inhibitory activity. Notably, saponins1–3inhibited pancreatic lipase (IC50= 0.11 ± 0.18 to 0.40 ± 0.21 mM) more strongly than the positive control orlistat, while also acting against α-glucosidase (IC50= 0.14 ± 0.11 to 0.23 ± 0.17 mM). Molecular docking supported their binding to the enzymes’ active sites. This study identifies1–3and8as dual α-glucosidase/pancreatic lipase inhibitors, underscoring the potential of adzuki bean as a source of bioactive functional food ingredients.
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PubMed
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CHEMISTRY & BIODIVERSITY
·
2026
·Vol. 23
·期 1
·e03315
·
IF 2.5
Shi-Hui Lu,Xiu-Xia Li,Xiao-Yan Xia,Wei-Neng Li,Kai-Jie Luo,Jing Huang
In the phytochemical study of the leaves of Ligustrum robustum , two new monoterpenoid glycosides named ligurobustosides V ( 1a ), V 1 ( 1b ), and a new secoiridoid glycoside named ligurobustoside W ( 2 ), together with three known glycosides ( 3 – 5 ), were isolated and identified by spectroscopic and chemical methods. The biological research discovered, compounds 3 and 4 showed powerful fatty acid synthase inhibitory activity (half inhibitory concentration [IC 50 ]: 4.21 ± 0.16–4.51 ± 0.12 µM); compounds 1 , 4 , and 5 displayed some α -glucosidase inhibitory activity; compounds 1 - 5 revealed some α -amylase inhibitory activity; compounds 1 , 3 , and 4 showed stronger 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical scavenging effect (IC 50 : 3.98 ± 0.01–6.80 ± 0.07 µM) than L-(+)-ascorbic acid (IC 50 : 10.06 ± 0.19 µM). Compounds 1 , 3 , and 4 were considered as some functional constituents of L. robustum to prevent diabetes mellitus and its complications. Graphical This article reports two new monoterpenoid glycosides and a new secoiridoid glycoside from the leaves of Ligustrum robustum; meanwhile describes their inhibitory capacities on alpha-glucosidase, alpha-amylase, fatty acid synthase, and their antioxidant effects.
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MICROBIAL PATHOGENESIS
·
2026
·
IF 3.5
Jingpin Gao,Mingxin Jiang,Jianlin Chen,Hongbo Zhang
Chlamydia trachomatis is a common sexually transmitted bacterial pathogen that often causes urogenital infection and may progress to chronic inflammation and fibrosis due to its clinically silent nature. Although azithromycin and doxycycline are widely used for treatment, therapeutic failure has been documented in clinical cases. In this study, we demonstrate that orlistat disrupts the chlamydial developmental cycle both in vitro and in vivo. Orlistat inhibits early stages of chlamydial infection, reticulate body replication, and reticulate body–to–elementary body differentiation. Transcriptomic analysis revealed significant downregulation of multiple genes associated with mid-to-late developmental stages, indicating disruption of the chlamydial developmental program. These findings suggest that orlistat interferes with chlamydial growth and development, although the underlying mechanisms have not been fully defined.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.1
Shiyu Li,Li Zhang,Haihua Zhang,Fang Yang,Jun Dang
Hyperlipidemia is closely linked to abnormal dietary lipid absorption, and pancreatic lipase (PL) is a key target for inhibiting intestinal lipid hydrolysis. Thus, the efficient discovery of PL inhibitors is crucial for treating the disease. However, existing recognition techniques suffer from false positives and disconnection from subsequent separation, hindering the exploration of natural product active components. This study developed an online high-performance liquid chromatography-fluorescence detection (HPLC-FLD) recognition system. It enables real-time mixing of chromatographic eluents with PL solution, forming protein-ligand complexes that yield characteristic negative peaks due to their lower fluorescence, thus enabling rapid localization of PL-binding components at corresponding retention times. Validation with orlistat as a reference standard confirmed the system's high precision, stability, and specificity. When applied to the crude extract of Sinacalia tangutica , this system recognized 15 active chromatographic peaks in a single analysis. Combined with an activity-guided progressive separation strategy, 16 compounds were obtained and exhibited PL inhibitory activity in vitro enzymatic assays. The therapeutic potential of phlorizin, one of inhibitors identified by our recognition system, was evaluated in hyperlipidemic mice. High-dose phlorizin lowered serum total cholesterol (TC) by 46.3% and triglyceride (TG) by 66.3%, reduced pancreatic PL content by 52.4%, raised fecal TG by 65.7%, alleviated hepatic steatosis and oxidative stress, with efficacy near orlistat. This integrated strategy of online recognition with activity-guided separation provides an efficient solution for the discovery of natural PL inhibitors with therapeutic potential.
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JOURNAL OF FOOD SCIENCE
·
2026
·Vol. 91
·期 8
·e71394
·
IF 4.1
Yizhuo Zhang,Jun Huang,Rongqing Zhou,Suyi Zhang,Yong Li,Rui Huang,Rui Liu,Chongde Wu
Litchi processing generates large quantities of seed by-products that are rich in starch and polyphenols but remain underutilized. In this study, the bioactivity and prebiotic potential of litchi seed vinegar (EHA-B-S1), a sustainable product derived from litchi seed by-products, were systematically evaluated. EHA-B-S1 displayed measurable in vitro antioxidant activity: total antioxidant capacity (4 mmol Trolox equivalents per mL), DPPH radical-scavenging of 50 µmol Vc equivalents per mL, and ABTS radical-scavenging activity of 1.0 mmol Vc equivalents per mL. EHA-B-S1 displayed moderate inhibitory capacity against α-amylase, α-glucosidase, and pancrelipase, and its performance was close to that of acarbose and orlistat. During probiotic-mediated fermentation, EHA-B-S1 rapidly acidified the fermentation environment, treatment resulted in higher measured biomass of Lactobacillus acidophilus , Lactobacillus rhamnosus , Lactiplantibacillus plantarum , and partially sustained Bifidobacterium longum compared with the control. The increase in metabolic activity showed higher short-chain fatty acid production, especially acetic acid, and expanded flavonoid diversity from 36 to 47 detected compounds; total flavonoid content increased to approximately 176% of the initial level. Network pharmacology analysis further predicted that these flavonoids may be associated with multiple metabolic and inflammatory pathways, providing hypotheses for future mechanistic studies. Collectively, the experimentally observed in vitro activities and fermentation-associated metabolite changes support further investigation of litchi seed vinegar as an upcycled bioactive fermented-food candidate, whereas its molecular targets, physiological effects, and health relevance require targeted cellular and in vivo validation.
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JOURNAL OF APICULTURAL RESEARCH
·
2023
·
IF 1.9
Olaoye Solomon Balogun,Anthonia Olufunke Oluduro,Zhiqiang Liu
Volatile oils of propolis obtained from Ondo (Ondo town) and Osun (Ile-Ife) States in Rain forest region of southwest Nigeria were extracted using the hydrodistillation method. The oils were analysed on GC/MS and investigated for DPPH scavenging activities, antibacterial potentials, and inhibitory activities on lipase and alpha glucosidase enzymes. Spectrophotometric methods were used for the DPPH scavenging and enzyme’ inhibitions assays while the agar well diffusion method was employed for the antibacterial test. GC/MS analysis of essential oils obtained from Ondo (BPO) and Ile-Ife (BPI) indicated 80 (97.82%) and 66 (99.31%) compounds respectively. Upon classification, both oils were rich in monoterpenes, sequisterpenes and non-isoprenoid hydrocarbons. Principal chemical components of BPO included trans-α-bergamotene (9.08%), α-copaene (5.83%), cadina-1,4-diene (4.04%), 2-methyl-5-propionylfuran (3.43%), 1,5,9,9-tetramethyl-1,4,7-cycloundecatriene (3.52%), benzaldehyde (2.98%), sulcatone (2.95%), γ-muurolene (2.51%), caryophyllene (2.40%), α-bisabolol (2.09%), α-eudesmol (2.07) and α-curcumene (2.06%) while those of BPI were trans-α-bergamotene (10.14%), citral (5.44%), α-copaene (5.25%), cadina-1,4-diene (5.09%), melonal (3.69%), sulcatone (3.56%), farnesol (2.71%), 3,5,9-trimethyl-deca-2,4,8-trien-1-ol (2.66%), α-curcumene (2.64%), 1,5,9,9-tetramethyl-1,4,7-cycloundecatriene (2.20%), and α-muurolene (2.20%). Both oils inhibited α-glucosidase and had DPPH scavenging activities better than the standards, however, anti-lipase activity was observed for BPI only. The oils also demonstrated broad spectrum of antibacterial activities.The volatile components of Nigerian bee propolis are being investigated for the first time, and the plethora of biological activities resulting from their diverse chemical composition showed that bee propolis could be a potential source for drug discovery.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Jinpeng Liu,Mashooq Khan,Yong Wei,Anna Liu,Tao Wang,Qiongzheng Hu,Li Yu
High-throughput screening of lipase inhibitors is critical for developing anti-obesity drugs. Herein, a robust and versatile sensing platform based on the difference in the sliding time of droplets on liquid crystal-infused porous surfaces (LCIPS) is developed to detect lipase inhibitors. Lipase catalyzes glycerol trioleate (GT) to produce oleate that can adsorb at the solution/liquid crystal (LC) interface due to its high amphiphilicity, which impedes the sliding of droplets on LCIPS, resulting in an extended sliding time. However, in the presence of lipase inhibitors, the enzymatic hydrolysis of GT by lipase is inhibited, which shortens the sliding time. The screening of three lipase inhibitors, orlistat, cetilistat, and epigallocatechin gallate (EGCG), popular anti-obesity drugs, is demonstrated to have IC 50 values of 17.45 ± 3.15 nM, 72.15 ± 6.32 nM, and 2.19 ± 0.24 μM, respectively. This study provides a simple, inexpensive, portable, user-friendly, and instrument-free method for rapid, accurate, and high-throughput screening of lipase inhibitors. The LCIPS-based sensing platform is up-and-coming in developing advanced bioanalytical sensors.
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Food & Function
·
2023
·Vol. 14
·期 10
·4763-4776
·
IF 6.1
Xinyue Zhang,Dan Li,Kexin Wang,Jiao Xie,Yaojie Liu,Tianxin Wang,Suwen Liu,Qun Huang,Qingbin Guo,Hao Wang
Hyperoside, the main component of many anti-obesity plants, might exhibit a lipase inhibition effect to reduce fat accumulation. The anti-obesity effect of hyperoside was investigated by studying its inhibitory effect and mechanism on pancreatic lipase in vitro and evaluating its ability to reduce lipid accumulation in vivo. Hyperoside is a mixed-type inhibitor of lipase with an IC50 of 0.67 ± 0.02 mmol L−in vitro. Hyperoside changed the secondary conformation of lipase, increased the α-helix content, and changed the microenvironment of lipase through static quenching. The interaction between hyperoside and lipase results from a strong binding spontaneous exothermic reaction, mainly through hydrogen bonding, van der Waals force and electrostatic force. Hyperoside protected hepatic lipid accumulation and adipose tissue hypertrophy and reduced the expression of inflammatory factors in high-fat diet-induced rats. Moreover, hyperoside had a good inhibitory effect on lipase activity in serum and increased fecal fat excretion, thereby reducing lipid absorption in vivo. This study provides theoretical support for the research and development of hyperoside in fat-reducing functional foods.
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Food & Function
·
2023
·Vol. 14
·期 10
·4807-4823
·
IF 6.1
Suwen Liu,Wenhong Jiang,Chang Liu,Shuo Guo,Hao Wang,Xuedong Chang
Chinese chestnut shell is a by-product of chestnut food processing and is rich in polyphenols. This study sought to investigate the effect of chestnut shell polyphenol extract (CSP) on weight loss and lipid reduction in a 12-week high-fat diet (HFD)-induced murine obesity model. CSP (300 mg per kg body weight) was administered intragastrically daily. AG490, a JAK2 protein tyrosine kinase inhibitor, was also intraperitoneally injected. The results showed that an HFD induced leptin resistance (LR). Compared to corresponding values in the HFD group, CSP treatment improved blood lipid levels, weight, and leptin levels in obese mice (p < 0.01). Additionally, CSP treatment enhanced enzyme activity by improving total antioxidant capacity, attenuating oxidative stress, and reducing fat droplet accumulation and inflammation in the liver, epididymal, and retroperitoneal adipose tissue. CSP also activated the LEPR-JAK2/STAT3-PTP1B-SOCS-3 signal transduction pathway in hypothalamus tissue and improved LR while regulating the expression of proteins related to lipid metabolism (PPARγ, FAS, and LPL) in white adipose tissue in the retroperitoneal cavity. However, the amelioration of lipid metabolism by CSP was dependent on JAK2. Molecular docking simulation further demonstrated the strong binding affinity of procyanidin C1 (−10.3983297 kcal mol−1) and procyanidin B1 (−9.12686729 kcal mol−1) to the crystal structure of JAK2. These results suggest that CSP may be used to reduce HFD-induced obesity with potential application as a functional food additive.
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Journal of Functional Foods
·
2023
·
IF 5.6
Tao Gao,Shuang Liu,Huang-yang Luo,Ren Wu,Rui Yan,Ling-zhi He,Qing-yu Nie,Hua-li Tang,Guiju Sun
Chuanminshen violaceum , as a food and folk medicine, is cultivated and grown only in China. Its polysaccharides play an important role in medicinal benefits. Herein, crude C. violaceum polysaccharides (CVPs) were purified to yield two polysaccharide fractions (CVPs-1-G and CVPs-2-G) using DEAE Cellulose-52 and Sephadex G-100 column chromatography. Structural analyses indicated that both CVPs-1-G and CVPs-2-G were glucans with average molecular weights of 8.1 kDa and 187.5 kDa, respectively. The main chain of the major component (CVPs-1-G) might be composed of (1 → 4)-linked α-Glc p , and the branched-chain might be an α-Glc p → 4)-α-D-Glc p -(1 → 6)-α-D-Glc p -(1 → 4)-α-D-Glc p , which connected to the main chain through the O-6 position of → 3,6)-α-D-Glc p -(1 →. In addition, CVPs-1-G displayed ABTS, DPPH, and hydroxyl radical scavenging activity, as well as competitive inhibitory activities of α-amylase and non-competitive inhibitory activities of lipase and pepsin. According to molecular docking, its inhibitory activity on digestive enzyme might be caused by hydrogen bond and hydrophobic interaction between them. Thus, CVPs exhibit strong potential for use in functional foods and pharmaceuticals.
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危险品化学品经营许可证（带存储）