ACS ES&T Water
·
2026
·
IF 4.3
Zijun Dong,Zhihao Han,Lulu Zhang,Xiaohui Sun,Chenyu Wang,Yifeng Yang
The pervasive use of antiviral drugs (ATVs) for treating viral infections (e.g., HIV, hepatitis, and influenza) has led to their widespread detection in aquatic environments. However, their incomplete removal in wastewater treatment plants (WWTPs) and associated ecological risks remain poorly quantified, particularly in megacities with high population density and medical facility density post-COVID-19. This study analyzed influent, effluent, and process-unit samples from 16 WWTPs and surface water samples from two major river basins via UPLC–ESI–MS/MS to systematically evaluate the occurrence, removal dynamics, and ecological risks of eight priority ATVs. Ecological risk quotients (RQs) were calculated using measured environmental concentrations (MECs) and predicted no-effect concentrations (PNECs) for aquatic organisms. Acyclovir (ACV) was ubiquitously detected in all WWTPs (197–21,334 ng/L) and rivers (139–431 ng/L), with WWTP removal rates varying drastically by process (15.6–99.9%). Oseltamivir (OS) dominated influents (18,251–21,334 ng/L) but was absent in surface waters, suggesting rapid environmental transformation. Risk assessment identified high-risk RQs (>1) for ACV and OS in effluents, threatening algae and fish. Hybrid processes (e.g., A2O+MBR) outperformed single-stage systems, yet overall ATV removal remained suboptimal (avg. 48.6%). This work underscores the urgent need for process optimization and regulatory frameworks to mitigate ATV-driven ecological hazards.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2022
·
IF 3.571
Xiaojiang Wang,Yukun Jiao,Haiyan Zhu,Yan Lu,Daofeng Chen
In the present study, a procyanidins-enriched fraction (PCE) from the rhizome of Fagopyrum dibotrys was obtained by anticomplement activity-guided fractionation. PCE could alleviate H1N1-induced ALI in mice by reducing weight loss, decreasing lung index, and regulating cytokine levels in lung tissue. PCE contained 76.5 ± 1.1% procyanidins with a mean degree of polymerization (mDP) of 5.24 ± 0.16. Meanwhile, thirty-three chemical constituents, including 27 procyanidins and 6 other compounds, were recognized by UPLC-Triple-TOF-MS/MS. Among them, twenty recognized procyanidins were composed of (epi)catechin with B-type link, while the rest consisted of (epi)catechin gallate . Furthermore, six compounds were obtained by preparative HPLC on a C 18 column (250 × 10.0 mm, 5 µm), and their structures were confirmed by mass spectrum (MS), nuclear magnetic resonance (NMR), and specific rotation. The structure-activity relationship analysis indicated that DP and galloylation were closely related to the anticomplement activity of procyanidins. The obtained results revealed that anticomplement procyanidins were one kind of the potentially effective materials of F. dibotrys against H1N1 influenza virus infection, and the in vivo efficacy of these compounds was worthy of further investigation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Cellular and Infection Microbiology
·
2022
·
IF 6.073
Cui Huan,Zhang Cheng,Zhang Chunmao,Cai Zhuming,Chen Ligong,Chen Zhaoliang,Zhao Kui,Qiao Sina,Wang Yingchun,Meng Lijia,Dong Shishan,Liu Juxiang,Guo Zhendong
Influenza virus is a serious threat to global human health and public health security. There is an urgent need to develop new anti-influenza drugs. Lentinan (LNT) has attracted increasing attention in recent years. As potential protective agent, LNT has been shown to have anti-tumor, anti-inflammatory, and antiviral properties. However, there has been no further research into the anti-influenza action of lentinan in vivo, and the mechanism is still not fully understood. In this study, the anti-influenza effect and mechanism of Lentinan were studied in the Institute of Cancer Research (ICR) mouse model. The results showed that Lentinan had a high degree of protection in mice against infection with influenza A virus, delayed the emergence of clinical manifestations, improved the survival rate of mice, significantly prolonged the middle survival days, attenuated the weight loss, and reduced the lung coefficient of mice. It alleviated the pathological damage of mice infected with the influenza virus and improved blood indices. Lentinan treatment considerably inhibited inflammatory cytokine (TNF-α, IL-1β, IL-4, IL-5, IL-6) levels in the serum and lung and improved IFN-γ cytokine levels, which reduced cytokine storms caused by influenza virus infection. The underlying mechanisms of action involved Lentinan inhibiting the inflammatory response by regulating the TLR4/MyD88 signaling pathway. This study provides a foundation for the clinical application of Lentinan, and provides new insight into the development of novel immunomodulators.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHARMAZIE
·
2021
·Vol. 76
·期 5
·195-201
·
IF 1.267
Dai Tianming,Wang Min,Wang Pengzhen,Dai Libing,Dai Renke,Meng Qingqi
The effects of eight oral anti-coronavirus drugs (lopinavir, ritonavir, chloroquine, darunavir, ribavirin, arbidol, favipiravir, oseltamivir) on the metabolism of four specific glycosides (polydatin, geniposide, quercitrin, glycyrrhizin) and on the activities of three major glycosidases
(β-glucosidase, α-rhamnosidase, β-glucuronidase) from gut microflora were explored in vitro and determined by LC-MS/MS. The metabolism of polydatin, geniposide, quercitrin and glycyrrhizin was significantly inhibited by one or several anti-coronavirus drugs of 100 μM
around 1 h and 4 h (P<0.05), among which darunavir could strongly reduce the production of genipin (70.6% reduction), quercitin (80.6% reduction) and glycyrrhetinic acid (37.9% reduction), which may cause a high risk of herb-drug interactions (HDI). Additionally, chloroquine reduced the
production of genipin and quercitin by more than 75% (P<0.05), whereas arbidol had no significant influence on the metabolism of polydatin, quercitrin and glycyrrhizin (P>0.05) so that its risk may be lower. The inhibition of darunavir on β-glucosidase was relatively strong (IC50
= 193±23 μM), and the inhibition became weaker on β-glucuronidase and α-rhamnosidase (IC50>500 μM). The consistency between gut microflora and glycosidase system indicated that the inhibition of darunavir on the activity of β-glucosidase and β-glucuronidase
may be the main reason for affecting the metabolism of geniposide, glycyrrhizin and polydatin in gut microflora. However, for the inhibition of darunavir and chloroquine on the metabolism of quercetrin, there was no correlation between gut microflora and α-rhamnosidase system. Assessing
the risk of HDI mediated by glycosidases in gut microflora may be conducive to the safety and efficacy of combining traditional herbal and Western medicine for the treatment of patients with Covid-19.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2021
·Vol. 86
·期 7
·2851-2860
·
IF 3.167
Jianyu Zhu,Qiang Li,Xuezhi Yu,Xiya Zhang,Hongfang Li,Kai Wen,Yuebin Ke,Suxia Zhang,Zhanhui Wang
Ribavirin (RBV) is an effective antiviral drug, whose use is prohibited in animal husbandry worldwide. In this work, a novel immunizing hapten of RBV, named Hapten 4, was designed by comparing the conformational and electronic properties of RBV and haptens based on computational chemistry. Hapten 4 was synthesized and conjugated with carrier proteins to produce monoclonal antibody (mAb). The obtained mAb 4C3 for RBV exhibited an IC 50 value of 6.24 ng/ml in an indirect competitive enzyme-linked immunosorbent assay (icELISA) and displayed no cross-reaction with five other antiviral drugs, including amantadine. The applicability of the developed icELISA was verified in chicken, with a calculated limit of detection of 4.23 µg/kg. The recoveries in spiked chicken were 79.2%–107.3% with a coefficient of variation less than 15.9%. The results indicated that the produced antibody from the new hapten was reliable and would be useful for RBV screening in chicken. Practical Application RBV is a broad-spectrum antiviral drug, which is commonly used illegally in poultry farms. A high-affinity mAb 4C3 against RBV was produced and used to develop icELISA with acceptable sensitivity and accuracy. The constructed icELISA has excellent performance for detecting RBV residues in chicken.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
·
2018
·
IF 2.129
Zhaopeng Wang,Xuezhi Yu,Licai Ma,Hebing Liu,Shuangyang Ding,Zhanhui Wang,Xiya Zhang,Jianzhong Shen,Kai Wen
In this work, high affinity polyclonal antibodies for ribavirin (RBV) from new haptens were prepared and were used to analyse RBV residues in chicken muscle, eggs and duck muscle. The new haptens were synthesised with different spacers, and the best antibody was obtained with an IC50 value as low as 0.61 ng/mL in indirect competitive enzyme-linked immunosorbent assay (ELISA). The cross-reactivities with another five antiviral drugs including amantadine, rimantadine, moroxydine, zanamivir and oseltamivir were less than 0.1%, which indicated the good specificity of the antibody. An ELISA was developed based on the antibody and applied to detect RBV in multi-food matrices. The sample preparation prior to detection only needed simple dilution after trichloroacetic acid extraction. The limits of detection were 1.07, 1.18 and 1.03 μg/kg in chicken muscle, eggs and duck muscle, respectively. Recoveries ranged from 89.0% to 112.7% with coefficients of variation below 13.0%. Ten blind samples of chicken muscle were analysed simultaneously by ELISA and liquid chromatography-tandem mass spectrometry, and a good correlation between the methods was observed. The results indicated that the high affinity antibody could be applied for the simple and fast detection of RBV in multi-food matrices.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
XiaoNa Su,JinYi Yang,QiLiang Li,XuQiong Yang,Ru-Yu Fang,YongYi Zhang,Li Chen,Feng Chen,YuanXin Tian,Yu-Dong Shen,Hong Wang
Amantadine (AMA), commonly used to treat viral infections in livestock and poultry, has been banned owing to its potential hazards to human well-being. To detect unauthorized AMA usage in livestock, we developed a polyclonal antibody with a high affinity for the specific recognition of AMA through a rational design based on a structure similar to AMA and revealed the availability of the hapten design by computational chemistry analysis. Using this antibody, we established a highly responsive time-resolved fluorescence immunochromatographic assay (TRFICA). The visual detection limit of the assay is 0.6 μg/kg, and the quantitative detection limit is 0.05 μg/kg. The TRFICA also showed good recovery rates ranging from 94.5 to 109.9%, with variability coefficients not exceeding 10%. The outcomes of undisclosed sample examinations aligned with those of HPLC-MS/MS analyses, indicating that this approach can function as an ideal screening and monitoring tool for detecting illegal AMA in chicken muscle.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2025
·
IF 5.4
Guoqi Yuan,Yang Liu,Zhou Hong,Haiyan Zhu,Yan Lu,Daofeng Chen
Ethnopharmacological relevance Bubali Cornu (BC) has a long history of use in traditional Chinese medicine for the treatment of various conditions, including high fever, hemoptysis, epistaxis, and infections. Despite its historical use, the active components responsible for its therapeutic effects, particularly in viral pneumonia, remain poorly understood. Aim of the study The objective of this study was to identify the active compounds in BC with antiviral activity against H1N1 and to evaluate their therapeutic potential in alleviating inflammation in H1N1-infected acute lung injury (ALI) mice. Materials and methods The antiviral components of BC were isolated using silica gel column chromatography and high-performance liquid chromatography (HPLC) according to a bioactive guided separation strategy. Then, the protective effects of these components in H1N1-induced ALI mice were evaluated. To explore the potential mechanisms, network pharmacology (NP) analysis and qPCR were used to screen the related signaling pathways or proteins. Finally, the functions of the most effective compound were verified in H1N1-infected RAW264.7 macrophages, focusing on its regulatory effects on key proteins. Results The ethyl acetate fraction (EAF) of BC had a protective effect against ALI induced by H1N1 in mice. Subsequently, 13 compounds were isolated from EAF of BC, of which nicotinic acid (NA) showed the best antiviral activity in vitro . In vivo studies showed that NA treatment of ALI mice infected with H1N1 significantly reduced lung index and inflammation, and improved lung tissue morphology. Flow cytometry analysis revealed that NA reduced the over-recruitment of macrophages and neutrophils to alleviate the inflammatory storm. As verified by NP and qPCR, NA reduced the mRNA levels of NOS2 and NOX1 in the lungs of mice with viral pneumonia, but Western blot analysis only showed that NA inhibited the expression of NOS2. Finally, NA significantly inhibited the expression of NOS2 in RAW264.7 cells infected with H1N1, suggesting that NA might alleviate ALI induced by H1N1 by inhibiting NOS2 expression in pulmonary macrophages. Conclusion The present study demonstrated that NA isolated from BC exhibited significant antiviral and anti-inflammatory properties in H1N1-induced ALI. NA might alleviate the progression of H1N1 pneumonia by inhibiting virus replication, reducing the over-recruitment of macrophages and neutrophils, and inhibiting the expression of NOS2. These findings provided valuable insights into the potential of BC in the treatment of viral infections, and highlighted the importance of exploring traditional animal-derived TCM in modern antiviral applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）