MICROCHEMICAL JOURNAL
·
2025
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IF 5.1
Shilan A. Fatah,Khalid M. Omer
Ofloxacin (OFL), a broad-spectrum fluoroquinolone antibiotic, is widely utilized for treating bacterial infections. Accurate detection of OFL is essential for pharmaceutical quality control and environmental monitoring, particularly in wastewater. This study introduces a novel fluorescence-based detection method using sumac-derived carbon dots (S-CDs), synthesized via a simple hydrothermal approach. The S-CDs exhibited spherical morphology (20–30 nm) with blue fluorescence emission and a quantum yield of 1.4 %. Upon interaction with OFL, a remarkable fluorescence enhancement was observed, resulting in a 45-fold increase in quantum yield (45.0 %) accompanied by a red shift in the emission. This strong enhancement and red-shift emission enabled to design off–on mode for detection of OFL. The fluorescence intensity showed a strong linear correlation with OFL concentration (R 2 = 0.98) in the range of 1–90 nM, with a limit of detection (LOD) of 0.7 nM and a limit of quantification of 2.3 nM. Furthermore, a smartphone-assisted visual detection method was developed based on an off–on fluorescence mechanism. RGB analysis enabled real-time quantification of OFL, exhibiting a linear detection range of 20–80 µM with an LOD 0.97 µM. The method was successfully applied to detect OFL in eye drop formulations and tap water, demonstrating high accuracy and precision. These findings underscore the potential of S-CDs as a cost-effective, portable, and efficient tool for OFL detection, suitable for pharmaceutical and environmental monitoring applications.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Fangfang Zhou,Yue Huang,Qian Wang,Yijie Wang
Antibiotic residue in food is still a serious health hazard to human with the various and severe adverse effects, so ultrasensitive detection of antibiotic residue becomes increasingly significant. Herein, a homogenous electrochemical biosensor is established for ultrasensitive detection of antibiotic ofloxacin (OFL) based on AuNPs-enhanced synergistic amplification of dual enzymes. Target OFL is ingeniously designed to initiate the synergistic effect of exonuclease III-catalyzed DNA cycle and terminal deoxynucleotidyl transferase-mediated polymerization, producing a great many polyA@AuNPs which subsequently adsorb onto the surface of gold electrode and finally lead to highly amplified electrochemical signal by binding with numerous electroactive indicator RuHex. Benefiting from the collaboratively multiple signal amplifications enhanced by AuNPs with good electrical conductivity and probe loading capacity, the constructed biosensor exhibits excellent quantitative detection performance for OFL with a linear range of 0.008 to 100 ng mL −1 and a limit of detection as low as 2.73 pg mL −1 . Furthermore, the detection system accomplished in homogeneous solution without complex chemical modification and rebuilding of sensing architectures on the electrode endows the biosensor with the advantages of convenient operation, good reproducibility and stability. Moreover, the biosensor shows satisfactory analytical performance for OFL detection in real food samples, providing a potential approach for practical application.
研究领域:
分析化学
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生物医学工程
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食品安全检测
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DOI
高影响因子
BIOMEDICAL CHROMATOGRAPHY
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2015
·Vol. 30
·期 3
·426-431
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IF 1.723
Jing Nie,Yang–Guang Wang,Xiao–Feng Gao,Xiao–kun OuYang,Li–Ye Yang,Di Yu,Wei–jian Wu,Hong–Ping Xu
( S )-(−)-Ofloxacin and ( R )-(+)-ofloxacin concentrations in the plasma of Pagrosomus major after drug treatment were detected by chiral high-performance liquid chromatography, and various pharmacokinetic parameters were calculated from these data. The elimination half-life of ( S )-(−)-ofloxacin was significantly shorter than that of the ( R )-(+) enantiomer. ( S )-(−)-Ofloxacin also had a significantly lower maximum plasma concentration, area under the concentration–time curve from zero to infinity, and mean residence time than ( R )-(+)-ofloxacin. However, the apparent volume of distribution and total body clearance of ( S )-(−)-ofloxacin were greater than those of ( R )-(+)-ofloxacin. The ratio of the ( S )-(−)- to ( R )-(+)-ofloxacin plasma concentration was always <1.0. Together, these data suggest that ( S )-(−)-ofloxacin was preferentially excreted and ( R )-(+)-ofloxacin was preferentially absorbed. Although the difference in pharmacokinetic parameters was small, the metabolic behavior of the ofloxacin enantiomers in P. major was enantioselective. Copyright © 2015 John Wiley & Sons, Ltd.
研究领域:
药理学
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分析化学
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海洋生物学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2025
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IF 11.3
Qian Guo,Zuxin Xu,Wenhai Chu,Yingying Zhou,Xiaoting Gao,Cheng Ye
Sewer overflows are a potential source of emerging contaminants to urban waters, posing a threat to ecosystems and human health. Herein, the performance and mechanism of ferrate(Ⅵ) (Fe(Ⅵ))/peroxymonosulfate (PMS), Fe(Ⅵ)/peroxydisulfate (PDS), and Fe(Ⅵ)/percarbonate (SPC) for the degradation of ofloxacin (OFL) in overflows were comparatively investigated. These systems achieved efficient degradation of OFL and the removal of conventional pollutants. Particularly, Fe(Ⅵ)/PMS showed better degradation performance for OFL with a degradation efficiency of 98.8 %. The dominant reactive oxygen species for OFL degradation in the Fe(Ⅵ)/PMS, Fe(Ⅵ)/PDS, Fe(Ⅵ)/SPC systems were singlet oxygen ( 1 O 2 ), sulfate radical (SO 4 · - ), and hydroxyl radical (·OH), respectively. High-valent iron species played an important role in the Fe(Ⅵ)/PMS and Fe(Ⅵ)/PDS systems. Notably, the synergistic effect of oxidation and in-situ coagulation played a key role in OFL degradation, which determined the superior performance of Fe(Ⅵ)/PMS. The formed flocs with Fe-O-P bond acted as a highway to promote the electron transfer from OFL to PMS, resulting in the efficient degradation of OFL in Fe(Ⅵ)/PMS system. Moreover, a same degradation pathway of OFL was found, and the toxicity of the degradation products was reduced, especially in the Fe(Ⅵ)/PMS system. This study provided a new strategy for overflows treatment.
研究领域:
环境科学
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化学工程
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水处理技术
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DOI
PubMed
高影响因子
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