Ling Zou,Yulan Tian,Xi Zhang,Jiaru Fang,Ning Hu,Ping Wang
This work describes an immunosensor for the determination of okadaic acid (OA) using a Love-wave sensor with the immunogold staining method. In this assay, OA-BSA was captured by the anti-BSA antibody (BSA-MAb) bond with staphylococcal protein A (SPA) on the sensor surface via Fc regions. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were employed to monitor the fabrication steps of immunosensor using the redox probe of K 3 Fe(CN) 6 . An indirect competitive immunoassay format was employed to detect OA after the immunosensor was prepared and gold nanoparticles (AuNPs) were introduced to amplify the phase signal. The results demonstrated that developed immunosensor was able to specifically respond to OA ranging from 10 to 150 ng/mL, providing limit of detection of 5.5 ng/mL. When applied to determine OA concentration in real sample, the results agreed well with those obtained by the commercial ELISA assay. The developed immunosensor is therefore suitable and has great application potential in detection of OA in shellfish.
Xianxin Qiu,Longjie Zhong,Ying Gan,Kaiqi Su,Shihui Hu,Ping Wang
Okadaic acid is a kind of fat-soluble diarrhetic shellfish poison existing extensively in shellfish. The rapid on site detection of shellfish contaminated by the toxin is a serious problem. Herein, a new method combining a kit with the Bionic e-Eye was developed for the rapid detection of okadaic acid on site. After optimization the kit reached a very low limit of detection, 0.19 μg L−1, and the linear detection range was 0.2–10 μg L−1. The cross reaction rate with dinophysistoxins 1 was 23.6%. The kit had a small cross reaction with other kinds of toxin like saxitoxin, gynotoxin 2 & 3 and pectenotoxins 2-b. The recovery range was about 75.78–102.56%, and the CVs were lower than 20%. A correlation analysis was conducted between the Bionic e-Eye and a microplate reader in PBS and shellfish backgrounds. The detection results of the real shellfish samples by the Bionic e-Eye exhibited great consistency with high performance liquid chromatography. The total time taken to perform the detection was less than 90 minutes. Above all, the method integrated kit with the Bionic e-Eye was very suitable for the rapid detection of okadaic acid on site.
危险品化学品经营许可证（带存储）