Frontiers in Veterinary Science
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2025
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IF 2.9
Jie Luo,Yiping Wang,Wenya Zheng,Chaolong Xu,Jianhua Chen,Ben Liu,Xiaoyue Wang,Manxin Fang
Introduction Mastitis outbreaks in dairy goats at a farm in western Jiangxi Province, have recently imposed significant economic losses on farmers. This study aimed to identify the causative pathogen and implement targeted control measures for early disease eradication. Methods Pathological autopsy was performed on diseased goats submitted for examination. Pathological mammary gland tissues and milk cistern fluid were collected aseptically for bacterial isolation and purification. The isolated pathogen was characterized through colony morphology observation, Gram staining, biochemical tests, and 16S rDNA gene sequencing. Pathogenicity was assessed using an animal challenge test in mice. In vitro antibacterial susceptibility testing was conducted using 30 Antibiotics (e.g., cefradine, cephalexin, cefuroxime), 18 Chinese herbal medicines (CHMs; e.g., Rhus chinensis (Galla Chinensis), Coptis chinensis (Chinese Goldthread), Punica granatum (Granati Pericarpium)), and 15 phytogenic extracts (e.g., Berberine, Gallic acid, Tannic acid). Results A Gram-positive bacterium was isolated from the diseased tissues. Biochemical profiling and 16S rDNA sequencing identified the isolate as Staphylococcus caprae . The animal challenge test confirmed the isolate’s strong pathogenicity in mice. Susceptibility testing indicated that the isolate was susceptible to Antibiotics such as cefradine, cephalexin, cefuroxime, and ciprofloxacin, while showing the poorest susceptibility to penicillin among the tested antibiotics. Among the CHMs and phytogenic extracts, Rhus chinensis (Galla Chinensis), Coptis chinensis (Chinese Goldthread), Punica granatum (Granati Pericarpium), Berberine, Gallic acid and Tannic acid demonstrated superior antibacterial efficacy. Discussion This study provides multiple therapeutic and preventive options (CHMs, Antibiotics, phytogenic extracts) for mastitis control on this farm. It also offers valuable insights into the identification and treatment of mastitis in dairy goats, contributing to safeguarding goat health and ensuring dairy product safety.
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DOI
PubMed
高影响因子
JOURNAL OF DAIRY SCIENCE
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2025
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IF 4.4
Huixin Dong,Xintong Zhang,Shuaishuai Li,Jingbo Ma,Jun Zhang,Hongyang Chen,Yudith lamothe Crespo,Junhu Yao,Zongjun Li
The objective of the current study was to evaluate the short-term effects of fumarate and active dry yeast (ADY) on ruminal fermentation, methane (CH 4 ) emissions, microbiota (composition and function), apparent total-tract digestibility, production performance, and blood metabolites in dairy goats using a 2 × 2 factorial design. Twenty-eight primiparous dairy goats were blocked and randomly assigned to 1 of 4 treatments: (1) control; (2) fumarate supplied at 34 g/d; (3) ADY supplied at 1.5 g/d; and (4) fumarate and ADY supplied in combination. The treatments lasted for 38 d. The results showed that fumarate supplementation reduced CH 4 production (L/d), yield (L/kg DMI), and intensity (L/kg milk yield) by 11.3%, 15.5%, and 21.9%, respectively, without affecting DMI, milk yield, or apparent nutrient digestibility. However, the CH 4 -depressing effect of fumarate began to decline 3 h postprandially and gradually diminished thereafter. Additionally, fumarate supplementation increased FCM with a higher NE L while reducing the ADG of goats with a lower NE G , resulting in no overall change in the net energy available for production. Fumarate supplementation also increased ruminal pH but reduced total VFA concentrations and microbial α diversity. Although fumarate did not alter the ruminal microbial structure at the genome or gene level, it stimulated the growth of fumarate- and lactate-utilizing bacteria, which may contribute to CH 4 mitigation and raise rumen pH. Supplementation with ADY increased the NE L and the molar proportion of ruminal butyrate and tended to increase CH 4 production and FCM without affecting CH 4 yield or intensity. Most of the blood metabolites and enzymes influenced by fumarate were involved in the tricarboxylic acid (TCA) and the urea cycles. Fumarate reduced the substrate concentrations of the TCA cycle (e.g., glucose, malondialdehyde) and the urea cycle (e.g., ammonia) in blood, but it increased the end products of the urea cycle (BUN, MUN) and enhanced the activity of malate dehydrogenase, a key enzyme in the TCA cycle. These findings indicate that fumarate can reduce enteric CH 4 emissions and enhance the TCA and the urea cycles in host animals.
研究领域:
畜牧学
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反刍动物营养
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甲烷减排
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微生物学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
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