FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Yue Pan,Lu Liu,Songfan Tian,Xiaodong Li,Muhammad Hussain,Chunmei Li,Lihong Zhang,Qiumei Zhang,Youbin Leng,Shilong Jiang,Shuyan Liang
In this study, we analyzed and compared the fat globule interfacial compositions and structures in human milk and three types of infant formulas (IF1: supplied with MFGM , IF2: without MFGM and soy lecithin , IF3: supplied with soy lecithin). The results suggested that the interfacial protein composition of IF1 was comparatively closer to human milk, but still lacked certain bioactive MFGM proteins including XO, ADPH and PAS6/7. Considering the interfacial phospholipid , we observed 23, 31, and 29 phospholipid species that could be used to distinguish human milk and infant formulas (IF1, IF2 and IF3). We also found that phosphatidylinositol (PI) and phosphatidylserine (PS), which can absorb lipase, phosphatidylethanolamine (PE), and phosphatidylcholine (PC) and connect functional fatty acids, were lacking in infant formulas. Moreover, the infant formulas had a smaller average particle size of 0.38 μm and a thicker interfacial layer that interacted with the casein micelles. Even if IF1 included MFGM, fat globules structures like those found in human milk did not form, and the majority of the MFGM was still in the aqueous phase in free form. Overall, the comprehensive analysis in this study could be used to simulate or mimic human milk lipids at the supramolecular level.
研究领域:
营养学
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食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 13
·4742-4745
·
IF 1.938
Liqiang Liu,Dezhao Kong,Changrui Xing,Xun Zhang,Hua Kuang,Chuanlai Xu
Lactoferrin (LF) content in infant milk powder has been strictly regulated by many governments and there is a need for convenient and reliable assays. Using hybridoma techniques, fourteen monoclonal antibodies (mAbs) active against LF were prepared. Two antibodies (mAb2 and mAb3), recognizing spatially distant epitopes of LF, were selected to establish a sandwich enzyme-linked immunosorbent assay (ELISA). A solution of mAb3 (1 μg mL−1) was coated onto micro-titer plates for LF capture while mAb2 labeled with horseradish peroxidase (2.2 μg mL−1) was used as a detection antibody. Under optimized conditions, the proposed sandwich ELISA was evaluated, and linearly responded to LF standards in a range of 5–600 ng mL−1 and the limit of detection was 3.23 ng mL−1. Lactoferrin samples were able to be determined after simple dilution, and the recovery in fortified milk powder averaged between 98% and 109%. The developed assay showed both high specificity (with no obvious cross-reactivity with related proteins) and reproducibility (the coefficient of variation ranged from 4.5% to 7.1%), indicating the utility of this sandwich ELISA in LF monitoring.
研究领域:
免疫学
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食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Shihuan Li,Jinhui Li,Senwei Feng,Linlin Bian,Zhuoting Liu,Yunfang Ping,Xu Wang,Ann Van Schepdael
The presence of fatty acid esters of 3-monochloropropane-1,2-diol (3-MCPDE), 2-monochloropropane-1,3-diol (2-MCPDE) and glycidyl (GE) in foods is a potential concern due to their toxicity, especially for infants. This study proposed a rapid, sensitive and environmentally friendly method for simultaneous determination of the 3-/2-MCPDE and GE in breast milk and infant formula using headspace solid-phase microextraction (SPME) and on-fiber derivatization combined with gas chromatography-tandem mass spectrometry (GC-MS/MS). The approach proved high sensitivity with detection limits of 0.10 μg/L and 0.15 μg/kg for MCPDEs in breast milk and infant formula, respectively. Mean recoveries were obtained ranging from 79.9 to 111.2% for breast milk and from 82.1 to 106.7% for infant formula, with relative standard deviation (RSD) of less than 9.5%. According to the levels of 3-MCPDE and GE determined in 30 breast milk and 23 infant formulas, the exposure to 3-MCPDE and GE may pose a potential health risk for infants from birth to <12 months fed by breast milk and infants from birth to 6 months fed by infant formula.
研究领域:
食品安全
·
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Zhengchao Shen,Xin Ma,Muhammad Mujahid Ali,Jiang Liang,Haixia Sui,Zhenxia Du
Herein, a modified indirect method was established for the determination of 3-monochloropropane-1,2-diol esters (3-MCPDEs) and glycidyl esters (GEs), employing magnetic solid phase extraction by boronic acid-functionalized magnetic nanoparticles to replace the traditional clean-up procedure. Compared with routine methods, it has been proved to be more sensitive with limits of detection in the range of 0.02–1.5 μg/kg and less susceptible to contamination of phenylboronic acid derivatives and fatty acid methyl esters. The proposed method was applied to analyze 42 samples covering the entire infant formula (IF) production chain. Results revealed that homogenization process contributed 79–91 % of the total growth of the contaminants due to the vegetable oil addition, while the following evaporation and spray-drying processes contributed 9–21 % of the total growth owing to involved heat treatment. The GE levels in final IF products exceeded the maximum level set by EU regulation 2020/1322, indicating quality safety concerns in the production chain.
研究领域:
食品安全
·
分析化学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Bochen Wei,Fengxia Zhang,Mengyu Wang,Shaobo Li,Muhammad Qamer Abbas,Yuping Wan,Zhenxia Du
To reduce the differences in phospholipids (PLs) of human milk and infant formula, PL-rich raw materials and finished milk powder from the infant formula production line were collected and the phospholipids profiles of raw materials were systematically analyzed by LC-Q-TOF based on phospholipid omics. 108 phospholipids were detected in infant formula, 73 of which were common to human milk, the content of the different phospholipid molecules varied. For the production of raw materials, whey protein concentrate and arachidonic acid powder have the most similar structure to human milk. By varying the ratio of each ingredient, formula can be made closer to human milk. The particle swarm optimization (PSO) algorithm was applied for the optimization of the ratio of raw materials to closely mimic the human milk. Based on the optimized ratio, the optimized formulas were prepared. PCA analysis proved that the optimized formulas were highly effective in mimicking human milk.
研究领域:
营养学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Omega
·
2022
·
IF 4.132
Qiaoling Yang,Bing Niu,Shuqing Gu,Jinge Ma,Chaomin Zhao,Qin Chen,Dehua Guo,Xiaojun Deng,Yongai Yu,Feng Zhang
To develop a rapid detection method for nonprotein nitrogen adulterants, this experiment sets up a set of point-scan Raman hyperspectral imaging systems to qualitatively distinguish and quantitatively and positionally analyze samples spiked with a single nonprotein nitrogen adulterant and samples spiked with a mixture of nine nonprotein nitrogen adulterants at different concentrations (5 × 10–3 to 2.000%, w/w). The results showed that for samples spiked with single nonprotein nitrogen adulterants, the number of pixels corresponding to the adulterant in the region of interest increased linearly with an increase in the analyte concentration, the average coefficient of determination (R2) was above 0.99, the minimum detection concentration of nonprotein nitrogen adulterants reached 0.010%, and the relative standard deviation (RSD) of the predicted concentration was less than 6%. For the sample spiked with a mixture of nine nonprotein nitrogen adulterants, the standard curve could be used to accurately predict the additive concentration when the additive concentration was greater than 1.200%. The detection method established in this study has good accuracy, high sensitivity, and strong stability. It provides a method for technical implementation of real-time and rapid detection of adulterants in milk powder at the port site and has good application and promotion prospects.
研究领域:
食品安全检测
·
光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Qiji Zhang,Liu Liu,Yue Jiang,Yan Zhang,Yifan Fan,Wenxin Rao,Xin Qian
Microplastics can be found almost everywhere and extensively expose to human. Infants are vulnerable, and the potential risks of microplastics may be greater for infants, however, infants' microplastic exposure is still poorly understood. Since milk powder makes up a significant portion of many infants’ diets, we investigated microplastic pollution in infant milk powder and calculated the microplastic exposure from milk powder, feeding bottles and milk powder preparation. On one hand, we studied 13 different types of milk powder with different packaging, processing systems, and milk sources. The boxed milk powder (7 ± 3 items/100 g) was more polluted with microplastics than the canned milk powder (4 ± 3 items/100 g). The inner packaging of the boxed milk powder was plastic and aluminum foil laminated, it emitted 8 ± 2 to 17 ± 1 items/100 g of microplastics, and might be a main source of microplastics in boxed milk powder. On the other hand, we found that when infants consume milk powder, the microplastic exposure from milk powder itself is little, exposure from feeding bottles is 6.8 times higher than that from milk powder and milk powder preparation is 1.7 times higher.
研究领域:
环境科学
·
公共卫生
·
儿科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Wei Wei,Jie Yang,Dan Yang,Xiangyu Wang,Zhenyu Yang,Qingzhe Jin,Manyi Wang,Jianqiang Lai,Xingguo Wang
We compared phospholipids (PLs), PL fatty acid (FA) composition, and milk fat globule size and structure in human milk (n = 120) from mothers of full-term and preterm infants during lactation (colostrum, transition, 1 mo, 2 mo, and 3 mo) and 8 brands of infant formulas. The absolute quantification of PLs was analyzed using 31P NMR spectroscopy. Sphingomyelin was the dominant PLs (35.01 ± 3.31%) in human milk, whereas phosphatidylcholine and phosphatidylethanolamine were the dominant PLs in infant formulas. The PL content in preterm milk increased during lactation, whereas that in term milk remained stable. Saturated FAs (mainly 16:0 and 18:0) were the most abundant (>60%) PL FA in both preterm and term milk and increased throughout lactation. The mean diameter of milk fat globules in infant formulas was much smaller than that found in human milk (200 nm vs 5.63 μm). Significant differences were observed between human milk and infant formulas with regard to PLs, suggesting that more research is needed to mimic the PL profile in infant formulas.
研究领域:
营养学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Yan Liu,Junying Zhao,Weicang Qiao,Baoyu Yang,Qian Liu,Yan Liu,Lijun Chen
The phospholipid (PL) composition and milk fat globule (MFG) structure are important for MFG membrane function. However, the effects of various processing methods on these factors remain unclear. In the present study, we compared the effects of different processing methods for infant formula (IF) production on PLs and MFGs. Homogenization and heat sterilization significantly decreased the number of PL species in raw milk (RM, 104). Heat sterilization and spray drying significantly reduced PL content in RM (160.3 ± 12.2 mg/L; p < 0.05). During processing, the average volume diameter (5.67 ± 0.81 μm) and zeta potential (−7.37 ± 0.27 mV) of RM were significantly reduced ( p < 0.05). After homogenization, PLs were distributed in a circular and dotted manner around the MFGs. After spray drying, PL fluorescent rings lost structural integrity. Therefore, the tested processing methods substantially damaged the PL composition and MFG structure. These results may provide a scientific basis for future IF development.
研究领域:
食品科学
·
营养学
✓ 已验证
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2018
·
IF 3.52
Yong Chen,Yifan Zhang,Xiaohou Wang,Jianqun Ling,Guanghua He,Lirong Shen
Cronobacter sakazakii ( Cs ) is a typical foodborne bacterium that infect powdered infant formula (PIF) worldwide. In this study, a recombinant antimicrobial peptide, branded as Funme peptide (FP)was applied to protect PIF from Cs contamination. The result from the antimicrobial activity assay showed that the minimum inhibitory concentration (MIC) of BMAP-27 peptide, FP and Ampicillin against Cs were 250.0, 125.0 and 15.6 μg/mL, respectively, indicating FP possessed higher MIC than that of Ampicillin, and lower MIC than that of BMAP-27. The minimum biofilm eradication concentration (MBEC) assay showed that FP at 2 × MIC (250.0 μg/mL) could completely eradicated Cs biofilms. The antibacterial activity of FP might be due to the increasing permeability and the release of cytoplasmic β-galactosidase of Cs . The results acquired from transmission electron microscopy and scanning electron microscopy indicated that FP induced the disruption and dysfunction of cell walls and membranes. Moreover, safety assay showed that FP had low cytotoxicity to human erythrocytes. The present study investigated the antibacterial effects and mechanisms of FP against Cs , providing promising evidence to apply this novel antimicrobial agent against Cs contamination in foods and food processing facilities.
研究领域:
微生物学
·
食品安全
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2016
·
IF 3.445
Faizan A. Sadiq,Yun Li,TongJie Liu,Steve Flint,Guohua Zhang,Lei Yuan,Zhipeng Pei,GuoQing He
The propensity for aerobic bacilli and allied genera to produce highly heat-resistant spores and thermally stable spoilage enzymes are major bacteriological issues faced by the dairy industry. Most of the enzymes are able to survive any heat treatment applied during the manufacture of milk powders and have the potential to remain active in milk powders and other dairy products during storage, and may explain some of the sensory and functionality defects reported in dairy products . Despite many reports on the occurrence of spore-forming bacteria in dairy products, knowledge about food quality related properties of many aerobic sporeformers is still scarce. Therefore, the aim of this study was to determine thermal resistance and spoilage potential of a large pool of mesophilic and thermophilic sporeformers, representing 738 isolates and 31 different RAPD groups, recently isolated from Chinese milk powders. Spore formers producing highly heat resistant spores (surviving 125 °C for 30 min) included 2 thermophiles ( Geobacillus thermoleovorans group and Geobacillus stearothermophilus ) and one mesophilic species ( Brevibacillus brevis ). Paenibacillus macerans showed the highest proteolytic activity followed by members of the Bacillus cereus group, Br. brevis , Bacillus subtilis , G. thermoleovorans group and Virgibacillus proomii . The highest lipase producing strains belonged to Bacillus licheniformis . Phospholipase activity was only shown by members of the B. cereus group and Brevibacillus parabrevis . Ten strains showed positive β -galactosidase activity, while, 4 strains showed positive haemolytic activity. B. licheniformis strains, despite belonging to one RAPD group or sub-group showed markedly different phenotypic characters which support the previous findings of heterogeneity in RAPD-based B. licheniformis groups. The results of this study will broaden the knowledge about the spoilage potential and thermal resistance of many strains of dairy origin.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2023
·
IF 4.3
Zhengchao Shen,Kexin Tian,Muhammad Mujahid Ali,Sifan Mei,Xin Ma,Jiang Liang,Feng Pan,Jianwen Li,Haixia Sui,Zhenxia Du
3-monochloropropane-1,2-diol esters (3-MCPDEs) and glycidyl esters (GEs) are food contaminants and have arisen continuous attention due to their toxicity, especially towards infants. Current risk assessment of these contaminants was mostly employing deterministic approaches, lacking quantitative characterization of the likelihood, incidence, or severity of the risks involved. Herein, 3-MCPDE and GE levels in 46 representative infant formulas (IFs) from Chinese market were determined by GC-MS/MS. Then, combining the occurrence data and consumption data from China National Food Consumption Survey, the Monte Carlo simulation-based probabilistic model for risk assessment of 3-MCPDEs and GEs in IFs from Chinese market was established. The results showed that all P90 (90th percentiles) hazard quotient values were below 1, demonstrating 3-MCPDEs didn't pose health risks to most populations aged 0–36 months old. However, for 0–12 months old groups, P10 (10th percentiles) margin of exposure values were all below 25000, indicating GEs may pose potential risks to 10% of this group. Uncertainty analysis revealed that the probabilistic model had considered uncertainties of model input and distribution, and realized refined assessment. This study is the first report on probabilistic assessment of 3-MCPDEs and GEs in IFs, which also provided references for the formulation of related regulatory limits in China.
研究领域:
食品安全
·
毒理学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）