SCIENCE OF THE TOTAL ENVIRONMENT
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2023
·
IF 9.8
Dan Li,Su Yan,Xiaoyu Yong,Xueying Zhang,Jun Zhou
Coal chemical wastewater (CCW) containing toxic and hazardous matters requires to be treated prior to discharge. Promoting the in-situ formation of magnetic aerobic granular sludge (mAGS) in continuous flow reactor process has a great potential for CCW remediation. However, long granulation time and low stability limit the application of AGS technology. In this study, Fe 3 O 4 /sludge biochar (Fe 3 O 4 /SC) with biochar matrix derived from coal chemical sludge were applied to facilitate the aerobic granulation in two-stage continuous flow reactors, containing separated anoxic and oxic reaction units (abbreviated as A/O process). The performance of A/O process was evaluated at various hydraulic retention times (HRTs) (42 h, 27 h, and 15 h). Magnetic Fe 3 O 4 /SC with porous structures, high specific surface area (BET = 96.69 m 2 /g), and abundant functional groups was successfully prepared by ball-milled method. Adding magnetic Fe 3 O 4 /SC to A/O process could promote aerobic granulation (85 days) and the removal of chemical oxygen demand (COD), ammonia nitrogen (NH 4 + -N) , and total nitrogen (TN) from CCW at all tested HRTs. Since the formed mAGS had high biomass, good settling ability, and high electrochemical activities, mAGS-based A/O process had high tolerance to the decrease of HRT from 42 h to 15 h for CCW treatment. The optimized HRT for A/O process was 27 h, at which Fe 3 O 4 /SC addition can result in the increase of COD, NH 4 + -N and TN removal efficiencies by 2.5 %, 4.7 % and 10.5 %, respectively. Based on 16S rRNA genes sequencing, the relative abundances of genus Nitrosomonas , Hyphomicrobium / Hydrogenophaga and Gaiella in mAGS accounting for nitrification, denitrification as well as COD removal were increased during aerobic granulation. Overall, this study proved that adding Fe 3 O 4 /SC to A/O process was effective for facilitating aerobic granulation and CCW treatment.
研究领域:
环境工程
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污水处理
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微生物学
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产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Lei Yang,Xu-Hui Wang,Shen Cui,Yong-Xiang Ren,Jie Yu,Ning Chen,Qian Xiao,Lin-Kai Guo,Rui-Hua Wang
A novel strain NP5 with efficient heterotrophic nitrification, aerobic denitrification and phosphorus accumulation ability was isolated and identified as Pseudomonas putida strain NP5. The removed ammonium and phosphate were mainly converted into intracellular components by assimilation, and negligible nitrification intermediates and N 2 O were accumulated during heterotrophic nitrification. In addition, the optimal conditions for nutrient removal were: succinate as carbon source, C/N 10, P/N 0.2, temperature 30 °C, salinity 0% and shaking speed 160 rpm. Besides, strain NP5 possessed an exceptional heavy metal and nanoparticles resistance. Cr 6+ was found to be the most toxic among the tested metals, and it could be removed simultaneously. Moreover, an obvious phosphorus release was observed under anaerobic condition, and repeated exposure to the anaerobic/aerobic conditions could significantly improve the nutrient removal. Furthermore, the successful expression of key enzymes for nitrogen and phosphorous removal provided additional evidence for possibility of simultaneous nitrification, denitrification and phosphorus removal.
研究领域:
环境微生物学
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废水处理
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生物修复
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DOI
PubMed
高影响因子
Processes
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2019
·Vol. 7
·期 11
·827
·
IF 1.963
Yong Li,Siyuan Zhao,Jiejie Zhang,Yang He,Jianqiang Zhang,Rong Ge
The aerobic denitrifying phosphate accumulating bacteria (ADPB) use NO3−as an electron acceptor and remove nitrate by denitrification and concomitant uptake of excessive phosphorus in aerobic conditions. Activated sludge was collected from the A2O aerobic biological pool of the sewage treatment plant at Hezuo Town, Chengdu City. The candidate ADPB strains were obtained by cultivation in the enriched denitrification media, followed by repeated isolation and purification on bromothymol blue (BTB) solid plates. The obtained candidates were further screened for ADPB strains by phosphorus uptake experiment, nitrate reduction test, metachromatic granules staining, and poly-β-hydroxybutyrate (PHB) staining. The 16 sedimentation ribosome deoxyribonucleic acid (16 S rDNA) molecular technique was used to determine their taxonomy. Further, the denitrification and dephosphorization capacities of ADPB strains were ascertained through their growth characteristics in nitrogen-phosphorus-rich liquid media. The results revealed a total of 25 ADPB strains screened from the activated sludge of the A2O aerobic biological pool of the sewage treatment plant at Hezuo Town. These strains belonged to two classes, four orders, and five genera. Among them, the strain SW18NP2 was a potentially new species in theAcinetobactergenus, while the strain SW18NP24 was a potential new species in thePseudomonasgenus at the time of their characterization. TheAcinetobacterwas the dominant genus. The obtained ADPB strains demonstrated a rich diversity. The ADPB strains had significant variations in denitrification and dephosphorization capacities. Twenty-three strains exhibited a total phosphorus removal rate of above 50%, and 19 strains exhibited a total nitrogen removal rate of above 50%. The strain SW18NP2 showed the best denitrifying phosphorus removal (DPR) capacity, with a dephosphorization rate of 82.32% and a denitrification rate of 73.73%. The ADPB in the A2O aerobic biological pool of the sewage treatment plant at Hezuo Town demonstrated a rich diversity and a strong DPR capacity.Keywords:aerobic denitrifying phosphate accumulating bacteria (ADPB);diversity;denitrifying phosphorus removal
研究领域:
环境微生物学
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污水处理
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DOI
高影响因子
WASTE MANAGEMENT
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2019
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IF 5.431
Jun Zhang,Yu Bao,Yu Jiang,Hong-Tao Liu,Bei-Dou Xi,Dun-Qiu Wang
To observe the effect of aeration strategies on the dissipation of fluoroquinolones (FQs) during aerobic composting and explore their dissipation pathways, 60-L composting and 0.5-L incubation experiments were carried out in this study. Three aeration strategies (windrow, static aeration, feedback aeration) were applied to remove two typical FQs (Norfloxacin (NOR) and Ofloxacin (OFL)) during the 60-L composting of sewage sludge with 5 mg kg −1 of FQs added. Then, three 0.5 L-sample groups were taken during the three phases of the 60-L composting matrixes without FQs under static aeration, and were inoculated separately at 35 °C, 55 °C and 40 °C after being added with 5 mg kg −1 of FQs. In each group, incubation was carried out for three treatments (sterilization + no aeration, sterilization + aeration, and no sterilization + aeration). The FQs in the sewage sludge were mainly removed in the mesophilic and thermophilic phases in all the aeration strategies. The removal efficiencies were high for the whole process: 89.6–95.4% for NOR and 87.2–95.4% for OFL. The order of removal efficiency of FQs was static aeration > feedback aeration > windrow. The combination of composting phases facilitated to the rapid dissipation of FQs, which reduced the half-life to about 1/6 to 1/5 of the values in each phase. In the mesophilic and thermophilic compost, biodegradation was the main pathway for the dissipation of FQs followed by irreversible adsorption. Irreversible adsorption and biodegradation provided similar removal efficiencies for the curing compost. The volatilization of FQs was non-negligible in all phases.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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2014
·Vol. 99
·期 5
·2431-2439
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IF 3.811
Yu Lei,Zhang Xiao-yu,Xie Tian,Hu Jin-mei,Wang Shi,Li Wen-wei
Reduction of azo dye methyl red coupled with aerobic respiration by growing cultures of Klebsiella oxytoca GS-4-08 was investigated. In liquid media containing dye and 0.6 % glucose in a mineral salts base, 100 mg l −1 of the dye are completely removed in 3 h under shaking conditions. The dye cannot be aerobically decolorized by strain GS-4-08 without extra carbon sources, indicating a co-metabolism process. Higher initial dye concentration prolonged the lag phase of the cell growth, but final cell concentrations of each batches reached a same level with range from 6.3 to 7.6 mg l −1 after the dye adaption period. This strain showed stronger dye tolerance and decolorization ability than many reported strains. Furthermore, a new intracellular oxygen-insensitive azoreductase was isolated from this strain, and the specific activity of enzyme was 0.846 and 0.633 U mg −1 protein in the presence of NADH and NADPH, respectively. N , N dimethyl- p -phenylenediamine and anthranilic acid were stoichiometrically released from MR dye, indicating the breakage of azo bonds accounts for the intracellular decolorization. Combining the characteristics of azoreductase, the stoichiometry of EMP, and TCA cycle, the electron transfer chain theory of aerobic respiration, and the possible mechanism of aerobic respiration coupled with azo reduction by K. oxytoca GS-4-08 are proposed. This study is expected to provide a sound theoretical basis for the development of the K. oxytoca strain in aerobic process for azo dye containing wastewaters.
研究领域:
微生物学
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环境科学
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生物化学
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DOI
PubMed
高影响因子
Fermentation-Basel
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2022
·Vol. 8
·期 6
·262
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IF 5.123
Jun Hao,Wen-Tao Sun,Chang-Rong Wu,Ming-Zhu Zhang,Guang-Hao Xia,Yu-Long Zheng,Chao Chen
Broussonetia papyriferaL. (paper mulberry) is an alternative woody plant, which can used to replace part of the protein feed for ruminants. Ensiling is an effective way to preserve fresh pasture and to solve the problem of stable storage and feed conversion of paper mulberry in the rapid growth period. However, low dry matter (DM), water-soluble carbohydrate, and lactic acid bacteria (LAB) reduce the quality of paper mulberry silage. This study assesses the influence of wilting time (0 h and 3.5 h; lighting: 3.43 × 104Lux) and three additives (Enterococcus durans, CL; cellulase, CE; and formic acid, FA) on the fermentation quality, aerobic stability, and bacterial community of whole plantB. papyriferasilage. The whole plantB. papyriferasample was mowed and wilted for 0 h and 3.5 h, and then had CL, CE, or FA added, followed by 60 days of ensiling. The results show all silage samples had high fermentation quality with pH below 4.2, ammonia-nitrogen below 100 g/kg DM, and no detectable butyric acid. The additives protected the DM and the crude protein from protease activity (p< 0.05), and CL was the most effective among them. Furthermore, wilting time influenced the silage’s bacterial communities, but overall, CL treatment had the greatest impact on bacterial communities. Wilting time and formic acid treatment significantly improved aerobic stability (p< 0.05).Enterococcuswas positively correlated with lactic acid (LA), while negatively correlated with LA andWeissella(p< 0.001). Enterococcuswas identified as the main driver of the whole plant paper mulberry ensiling process in the present study. In conclusion, compared to other additives, LAB is the most effective and economical to improve the fermentation quality and reduce the protein degradation of whole plant paper mulberry silage. Our findings provide a theoretical basis to improve the quality and production of paper mulberry silage.Keywords:paper mulberry;silage;wilting;additives;microbial community;aerobic stability
研究领域:
动物营养学
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微生物学
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产品关联
DOI
高影响因子
International Journal of Environmental Research and Public Health
·
2017
·Vol. 15
·期 1
·35
·
IF 2.101
Shixiong Sheng,Bo Liu,Xiangyu Hou,Bing Wu,Fang Yao,Xinchun Ding,Lin Huang
This study investigated the biodegradation performance and characteristics of Sudan I and Acid Orange 7 (AO7) to improve the biological dye removal efficiency in wastewater and optimize the treatment process. The dyes with different water-solubility and similar molecular structure were biologically treated under aerobic condition in parallel continuous-flow mixed stirred reactors. The biophase analysis using microscopic examination suggested that the removal process of the two azo dyes is different. Removal of Sudan I was through biosorption, since it easily assembled and adsorbed on the surface of zoogloea due to its insolubility, while AO7 was biodegraded incompletely and bioconverted, the AO7 molecule was decomposed to benzene series and inorganic ions, since it could reach the interior area of zoogloea due to the low oxidation-reduction potential conditions and corresponding anaerobic microorganisms. The transformation of NH3-N, SO42−together with the presence of tryptophan-like components confirm that AO7 can be decomposed to non-toxic products in an aerobic bioreactor. This study provides a theoretical basis for the use of biosorption or biodegradation mechanisms for the treatment of different azo dyes in wastewater.Keywords:azo dye;aerobic biodegradation;Acid Orange 7;Sudan I;solubility
研究领域:
环境科学
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生物学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL TECHNOLOGY
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2023
·
IF 2.8
Hongxu Bao,Min Liu,Xianhui Li,Nanqi Ren,Jianzheng Li
A packed-bed anaerobic-aerobic reactor (PBAOR) with two anaerobic and two aerobic compartments was constructed to treat manure-free piggery wastewater which was characterized by high ammonium (NH4+-N) and low ratio of chemical oxygen demand (COD) to total nitrogen (TN). Performed for 60 days at the normal atmospheric temperature of 25 °C with a constant hydraulic retention time of 32 h and reflux ratio of 2.0, a stable state in pollutants removal was obtained in the PBAOR. Within the next routine operation process, the removal of COD, NH4+-N and TN was above 85.7%, 98.2% and 85.8%, with a residual less than 81.7, 7.2 and 39.9 mg L−1 in effluent, respectively. Twelve veterinary antibiotics classified into tetracyclines (TCs), sulphonamides (SAs) and fluoroquinolones (FQs) were detected from the piggery wastewater. The PBAOR was effective in removing TCs and SAs with an average removal of 74.8% and 93.3%, respectively, but presented a negative removal for FQs. Most COD in the piggery wastewater was mainly removed in the first two anaerobic compartments along with an obvious removal of TCs and SAs, while the TN were mainly removed in the last two aerobic compartments with the negative removal of FQs.
研究领域:
环境科学
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水处理工程
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畜牧学
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DOI
PubMed
高影响因子
Frontiers of Environmental Science & Engineering
·
2021
·Vol. 16
·期 6
·1-10
·
IF 4.357
Man Quanli,Zhang Peilian,Huang Weiqi,Zhu Qing,He Xiaoling,Wei Dongsheng
A novel salt-tolerant heterotrophic nitrification and aerobic denitrification (HN-AD) bacterium was isolated and identified as Halomonas venusta TJPU05 ( H. venusta TJPU05). The nitrogen removal performance of H. venusta TJPU05 in simulated water (SW) with sole or mixed nitrogen sources and in actual wastewater (AW) with high concentration of salt and nitrogen was investigated. The results showed that 86.12% of NH 4 + -N, 95.68% of NO 3 − -N, 100% of NO 2 − -N and 84.57% of total nitrogen (TN) could be removed from SW with sole nitrogen sources within 24 h at the utmost. H. venusta TJPU05 could maximally remove 84.06% of NH 4 + -N, 92.33% of NO 3 − -N, 92.9% of NO 2 − -N and 77.73% of TN from SW with mixed nitrogen source when the salinity was above 8%. The application of H. venusta TJPU05 in treating AW with high salt and high ammonia nitrogen led to removal efficiencies of 50.96%, 47.28% and 43.19% for NH 4 + -N, NO 3 − -N and TN respectively without any optimization. Furthermore, the activities of nitrogen removal-related enzymes of the strain were also investigated. The successful detection of high level activities of ammonia oxygenase (AMO), hydroxylamine oxidase (HAO), nitrate reductase (NAR) and nitrite reductase (NIR) enzymes under high salinity condition further proved the HN-AD and salt-tolerance capacity of H. venusta TJPU05. These results demonstrated that the H. venusta TJPU05 has great potential in treating high-salinity nitrogenous wastewater.
研究领域:
微生物学
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环境科学
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DOI
高影响因子
Journal of Water Process Engineering
·
2025
·
IF 6.7
Yanzhuo Zhang,Jiaqi Xu,Suya Wang,Xiaoya Wang,Jing Zhao,Xiaozhuan Zhang,Tonghuan Yu,Alexandr V. Bildyukevich
The aging of biochar by microorganisms was a long-term process. During this process, microbial growth and metabolism inevitably influenced the removal of organic pollutants from the biochar. Aging biochar through aerobic composting more closely simulated the natural microbial processes. The addition of hydrochar (HBC) and microplastics (MPs) had minimal impact on temperature, humidity, moisture content, and organic matter levels during the composting process. Antibiotic degradation by HBC/peroxymonosulfate (PMS) and (preparation of hydrochar from sludge and microplastics, HBC + MPs)/PMS initially decreased but later increased during compost aging, with the highest degradation efficiencies observed on day 30 of compost aging at 95.93 % and 96.30 %, respectively. The concentration changes in environmentally persistent free radicals (EPFRs) in hydrochar were consistent with the antibiotic degradation process. The concentrations of HBC-30 and HBC + MPs-30 were 1.52 × 10 17 and 1.66 × 10 17 spins/g, respectively. The degradation of antibiotics by HBC/PMS mainly occurred through a free radical pathway, and sulfate radicals (•SO 4 •− ) and hydroxyl radicals (•OH) played key roles in the degradation process. Microbial diversity analysis revealed that the dominant bacteria were Firmicutes, Proteobacteria, and Bacteroidota, collectively accounting for over 90 % of the total population. The biotoxicity test demonstrated that the degradation of antibiotics by hydrochar/PMS significantly reduced the biotoxicity of antibiotics in water. Moreover, the MPs were non-toxic and served as carriers for the growth of Scenedesmus obliquus . The aging process of aerobic compost enhanced the hydrochar/PMS degradation of antibiotics, which suggested that hydrochar could be effectively used over the long term.
研究领域:
环境科学
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微生物学
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污染控制
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产品关联
DOI
高影响因子
JOURNAL OF BASIC MICROBIOLOGY
·
2021
·Vol. 61
·期 5
·419-429
·
IF 2.281
Jie Chen,Juan Wu,Luosheng Fan,Rong Jia
The study investigated the characteristics of aerobic degradation of tetrabromobisphenol A (TBBPA) by Irpex lacteus F17 ( I. lacteus F17) under four different cometabolic substrates (phenol, glucose, sodium pyruvate, and sodium citrate). The biodegradation of TBBPA by I. lacteus F17 could be enhanced via cometabolism, and glucose (8 g/L) was confirmed to be the optimum carbon source. For different initial solution pH ranging from 3.0 to 8.0, the results showed that I. lacteus F17 could be applied to biodegrade TBBPA in a wide pH range of 4.0–8.0, and the degradation rate could reach the maximum 75.31%, while the debromination rate reached the maximum 12.40% under pH 5.0. In addition, it has been confirmed that Mn 2+ (50 μmol/L) could promote the secretion of manganese peroxidase and TBBPA biodegradation efficiency. Seven intermediates were identified by gas chromatography–mass spectrometry analysis, and the possible degradation pathways were proposed, which indicated the biodegradation of TBBPA might be subjected to debromination, β -scission, hydroxylation, deprotonation, and oxidation reactions.
研究领域:
环境生物学
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微生物学
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DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Xinyi Tao,Kun Zou,Mengze Yuan,Yixuan Wen,Benben Liu,Taiping Qing,Bo Feng,Peng Zhang
Microbial synthesis is an effective method for carbon and nitrogen recovery in the biological nitrogen removal process. The recycling of alginate-like exopolymers has attracted the attention of many researchers; however, the composition of the polymers and alginate differs. In this study, the capacity and mechanism of a mucoid Pseudomonas aeruginosa to remove nitrate and synthesize alginate were investigated. The results showed that the P. aeruginosa achieved higher nitrate removal efficiency (95.3 %) and alginate yield (298 mg/L) after 36 hours of microaerobic cultivation compare to aerobic condition. The optimal conditions for nitrogen removal and alginate recovery are 35 °C, pH 7 and C/N 15. The transcriptional activities of denitrifying genes in P. aeruginosa were upregulated at the low dissolved oxygen level. Although O 2 may upregulate the expression of alginate synthesis key genes ( algD , alg8 and alg44 ) by activating AlgZ-AlgR two-component signal transduction system, microaerobic condition facilitates P. aeruginosa proliferation and results in a higher alginate yield. Moreover, the P. aeruginosa strain was further coupled with an anoxic/aerobic reactor for deeper nitrogen removal, and the nitrate removal efficiency and alginate yield were maintained above 91 % and 100 mg/L, respectively. The conversion efficiency of sodium acetate to alginate reached 15 %. This work provides an economically and environmentally sustainable strategy for wastewater treatment and the recovery of high-value bioresource.
研究领域:
环境生物学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）