American Journal of Translational Research
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2025
·Vol. 17
·期 3
·2005
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IF 1.6
Xiaolan Zhuang,Xingxing Li,Shikai Yu,Lin Zeng,Yuping Xu,Tingting Hu,Sailong Wan
Objective: This study aimed to evaluate the clinical efficacy of labetalol (LBT) combined with magnesium sulfate (MgSO 4 ) in improving symptoms and pregnancy outcomes in patients with hypertensive disorders of pregnancy (HDP). Methods: A total of 122 HDP patients admitted between January 2020 and June 2024 were included. Among them, 56 patients (control group) received MgSO 4 alone, while 66 patients (research group) received a combination of LBT and MgSO 4 . Comparative analyses were conducted to evaluate therapeutic effectiveness, symptom improvement (edema, hypertension, and proteinuria), brain-related symptoms (nausea and vomiting, sluggishness, blurred vision, dizziness, and headache), pregnancy outcomes (placental abruption, neonatal asphyxia, fetal distress, and postpartum hemorrhage), blood pressure indicators (systolic (SBP) and diastolic blood pressure (DBP)), fetal umbilical artery hemodynamics (systolic-to-diastolic flow velocity ratio (S/D) and pulsatility index (PI)), and high-sensitivity C-reactive protein (hs-CRP). Results: The research group demonstrated significantly better outcomes than the control group, including a higher overall therapeutic effectiveness rate, faster resolution of edema, hypertension, and proteinuria, a lower incidence of adverse pregnancy events, and more pronounced reductions in SBP, DBP, S/D, PI, and hs-CRP levels after treatment (all P < 0.05). Conclusion: The combination of LBT and MgSO 4 is highly effective in improving symptoms and pregnancy outcomes in HDP patients, outperforming MgSO 4 monotherapy. This approach has strong potential for broader clinical application.
研究领域:
妇产科学
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药理学
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DOI
PubMed
高影响因子
INORGANICA CHIMICA ACTA
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2021
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IF 2.545
Yan-Lan Sun,Fen-Mei Zhou,Zhen-Jing Chi,Yu-Lei Zhang,Rui-Rui Zhang
This article has been retracted: please see Elsevier Policy on Article Withdrawal ( https://www.elsevier.com/locate/withdrawalpolicy ). This article has been retracted at the request of the editors. In investigating concerns brought up regarding the authenticity of the article, the editors reached out to the corresponding author for an explanation. Despite several attempts in requesting clarification on the concerns, the authors have not replied to the editors’ requests. The editors therefore feel that the findings of the manuscript cannot be relied upon and that the article needs to be retracted.
研究领域:
医学
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药理学
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生物化学
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DOI
高影响因子
HUMAN & EXPERIMENTAL TOXICOLOGY
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2022
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IF 3.247
Q Yang,Y Mao,J Wang,H Yu,X Zhang,X Pei,Z Duan,C Xiao,M Ma
Lipid metabolism is an important biochemical process in the body. Recent studies have found that environmental endocrine disruptors play an important role in the regulation of lipid metabolism. Bisphenol A (BPA), a common environmental endocrine disruptor, has adverse effects on lipid metabolism, but the mechanism is still unclear. This study aimed to investigate the effects of gestational BPA exposure on hepatic lipid metabolism and its possible mechanism in male offspring. The pregnant Sprague-Dawley rats were exposed to BPA (0, 0.05, 0.5, 5 mg/kg/day) from day 5 to day 19 of gestation to investigate the levels of triglyceride (TG) and total cholesterol (TC), and the expression of liver lipid metabolism-related genes in male offspring rats. The results showed that compared with the control group, the TG and TC levels in serum and liver in BPA-exposed groups was increased. And the expressions of liver fatty acid oxidation related genes, such as peroxisome proliferators-activated receptor α (PPARα) and carnitine palmitoyl transferase 1α (CPT1α), were down-regulated. However, the expressions of fatty acid synthesis related genes, such as sterol regulatory element binding proteins 1 (SREBP-1), acetyl-CoA carboxylase 1 (ACC1), fatty acid synthase (FAS) and stearoyl-CoA desaturase 1 (SCD-1), were up-regulated. The increased protein levels of mTOR and p-CRTC2 suggested that CREB-regulated transcription coactivator 2 (CRTC2) might be an important mediator in the mTOR/SREBP-1 pathway. In conclusion, these results demonstrated that mTOR/CRTC2/SREBP-1 could be affected by gestational BPA exposure, which may involve in the lipid metabolic disorders in later life.
研究领域:
环境健康
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内分泌学
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代谢
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DOI
PubMed
高影响因子
BMC Pregnancy and Childbirth
·
2023
·Vol. 23
·期 1
·1-10
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IF 3.1
Ding Ailing,Wan Huimin,Peng Juan,Wang Huizi,Zhu Shaodan,Dong Xudong
Background Hypertensive disorders complicating pregnancy (HDCP) and gestational diabetes mellitus (GDM) can affect the placental barrier function to varying degrees. However, current studies show that the transfer and distribution characteristics of trace elements in the maternal-fetal system are still unclear. This study investigated the effect of the placental barrier on the transfer of trace elements from mother to fetus and its relationship with HDCP and GDM. Methods A case-control method was used in this study. 140 pairs of samples were collected; 60 were from healthy pregnant women, and 80 were from patients with pregnancy complications. The contents of trace elements in paired samples were determined by inductively coupled plasma–mass spectrometry (ICP-MS). SPSS software was used to analyze the differences in trace element levels in matched samples of each group. The correlations were analyzed based on Pearson’s correlation factor (r). Results The distribution characteristics of Fe content in the pathological group (HDCP group and GDM group) were the same as those in the normal group (umbilical cord blood > maternal blood > placenta), but there was no significant difference in the iron content in maternal blood and cord blood of pathological group. The distribution characteristics of Mn content in the pathological group (placenta > umbilical cord blood > maternal blood) were changed compared with those in the normal group (placenta > maternal blood > umbilical cord blood). In addition, the placental Cr content and cord blood Cr and Ni content of the pathological group were higher than those of the normal group. HDCP placental Cr and GDM placental Fe levels were significantly correlated with the Apgar score. Conclusions The transfer of Fe and Mn and the placental barrier function of Cr and Ni in the maternal-fetal system of HDCP and GDM are significantly altered, which directly or indirectly increases the maternal and fetal health risk.
研究领域:
妇产科学
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营养学
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病理学
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DOI
PubMed
高影响因子
Orphanet Journal of Rare Diseases
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2021
·Vol. 16
·期 1
·1-13
·
IF 4.123
Shao Yong,Chen Siyu,Li Huan,Tang Qin,Xu Di
Background ICP pregnant women have a unique profile of serum bile acid metabolism, thus the early and accurate identification of ICP patients is beneficial to early appropriate treatment and improvement of pregnancy outcomes. In this study, ultra-high performance liquid chromatography-mass spectrometry/mass spectrometry (UPLC-MS/MS) was used to analyze the 15 types of serum bile acid profiles among patients with ICP in third trimester, patients with cholelithiasis, and patients with hepatitis B virus. The ICP diagnostic model established by partial least squares-discriminant analysis (PLS-DA) was used to screen the differential bile acids for clinical subtypes of ICP. 144 cases of ICP patients were involved in this study, and divided into four subgroups according to serum level of TBA, DBIL, and ALT.Results (1) The differential serum bile acid profiles of ICP group and normal pregnant women were DCA, TDCA, TCA, GDCA and GLCA. (2) The differential serum bile acid profiles of the ICP1 group (ICP with jaundice) and normal pregnant women were TCDCA, TCA, GCA, GCDCA, TUDCA and GUDCA. (3) The differential serum bile acid profiles of the ICP3 group (Hyperchoicemia of pregnancy) and normal pregnant group was GUDCA, LCA, GLCA, UDCA, TUDCA, CDCA, and TLCA (P < 0.05). (4) The differential serum bile acid profiles of ICP4 group (idiopathic aminotransferase abnormality during pregnancy) and normal pregnant group was UDCA, GUDCA, TUDCA, GCA and GLCA (P < 0.05). (5) The occurrence of meconium-stained amniotic fluid, premature delivery and cesarean section in ICP1 group was significantly higher than normal group, ICP2 group, ICP3 group, and ICP4 group (P < 0.05); The occurrence of meconium-stained amniotic fluid, premature delivery and cesarean section in ICP2 group, ICP3 group, and ICP4 group was significantly higher than normal group (P < 0.05), but no difference was found among ICP2 group, ICP3 group, and ICP4 group (P > 0.05).Conclusion Maternal serum bile acid profiles are useful to differentiate the four subtypes of ICP. ICP with jaundice could be an important predictor of adverse pregnancy outcomes of ICP.
研究领域:
妇产科学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NUTRITION
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2025
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IF 3.8
Lemei Li,Jinyang Wu,Huilei Hao,Ying Wang,Shaoying Hou
Background Gestational diabetes mellitus (GDM) is prone to induce hepatic injury in offspring. Sialic acid (SA) exhibits hepatoprotective effects, while its potential against GDM-induced liver injury in offspring remains unclear. Objective The present study aimed to investigate the hepatoprotective effects of maternal sialic acid (SA) supplementation in GDM-exposed offspring and to explore its underlying mechanisms. Methods C57BL/6J mice were divided into three groups: a control (NC-m) group, a gestational diabetes mellitus (GDM-m) group, and a gestational diabetes mellitus plus sialic acid (GDM+SA-m) group. The GDM-m group was induced using high-fat diet combined with intraperitoneal streptozotocin (STZ) injection. The GDM+SA-m group was administered N-glycolylneuraminic acid (Neu5Ac), human milk SA, via intragastric gavage at a dose of 50 mg/kg during pregnancy and lactation. Offsprings from three groups were fed a control diet until 8 weeks of age, with 20, 20, and 19 in the NC-o, GDM-o, and GDM+SA-o groups, respectively. Liver was obtained and analyzed for HE staining, AST and ALT, oxidative stress markers, inflammatory cytokines, and non-targeted metabolomics. Results and Conclusion s: Compared with the NC group, GDM significantly elevated liver transaminase levels and increased inflammatory and oxidative stress in offspring mice, with more pronounced effects observed in males. SA intervention partially attenuated this damage. Concurrently, metabolomic analysis revealed that SA significantly regulated lipid metabolism and taurine/hypotaurine pathways. The results suggested that maternal SA intervention ameliorated GDM-induced offspring liver injury potentially by regulating hepatic lipid and taurine/hypotaurine metabolism, providing a novel mechanistic insight for early-life intervention in GDM.
研究领域:
生殖医学
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发育生物学
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肝脏病学
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代谢组学
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围产期医学
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分子营养学
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氧化应激
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产品关联
DOI
PubMed
高影响因子
Heliyon
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2024
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IF 3.4
Maomao Sun,Congrong Wu,Jie Jiang,Yue He,Sha Zhu,Yonghui Yu
Background Maternal hypertensive disorders of pregnancy (HDP) was associated with increased risk of congenital hypothyroidism in preterm infants, but its underlying mechanisms remain unclear. Objective To investigate the possible mechanisms by which intrauterine exposure to HDP affects thyroid hormone synthesis in preterm infant rats. Methods preterm infant rats were obtained by Caesarean section delivery from the L-NAME group and Control groups which was induced by L-NAME and saline, respectively. Thyroid hormone levels of preterm infant rats were detected by ELISA, and morphology structure were observed by H&E staining and electron microscopy, and the expression of key factors of thyroid hormone synthesis and endoplasmic reticulum stress indicators were analyzed by RT-qPCR and Western blotting. Results Compared with the Control group, significantly higher serum TSH concentration was observed in the L-NAME group (p < 0.05), while T3 and T4 levels showed no noticeable change. The L-NAME group revealed a reduction in the size and number of thyroid follicles, with the emergence of thyroid follicular epithelial hyperplasia. While electron microscopy revealed that the endoplasmic reticulum of thyroid follicular epithelial cells was marked swollen within L-NAME group. Additionally, the mRNA expression of Ttf1 , Pax8 and Tshr was down-regulated in thyroid tissues of L-NAME group. Furthermore, the protein levels of Tg, NIS and TSHR were reduced, while the protein level of p-IRE1α, ATF6α, XBP1s and Bip were increased in the L-NAME group. Conclusion The results indicated that HDP may reduce the expression of key molecules involved in thyroid synthesis through endoplasmic reticulum stress which could ultimately result in the development of congenital hypothyroidism.
研究领域:
医学
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内分泌学
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DOI
高影响因子
Frontiers in Endocrinology
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2025
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IF 4.6
Congrong Wu,Maomao Sun,Yue He,Jie Jiang,Luran Wang,Yanni Wang,Yonghui Yu
Background: Clinical research has identified a correlation between hypertensive disorders of pregnancy (HDP) and subclinical hypothyroidism during gestation. But the potential influence of HDP on thyroid hormone synthesis remains undetermined.Aims: This study aims to elucidate the impact of HDP on thyroid hormone synthesis and to delineate the underlying mechanisms.Methods: 20 pregnancy SD rats were stratified at random into the HDP group and the Control group. The HDP group was subjected to NG-Nitro-L-arginine-methylester administration from gestational days 13 to 20, while the Control group received saline. Subsequent assessments encompassed serum FT4, FT3, and TSH concentrations, morphological examination of the thyroid, as well as quantification of essential proteins pivotal to thyroid hormone synthesis and markers indicative of endoplasmic reticulum stress.Results: The HDP group exhibited a statistically significant augmentation in serum TSH concentrations (p<0.05), while FT3 and FT4 levels manifested no discernible statistical variations. H&E staining highlighted a pronounced hyperplasia of the follicular epithelial cells and a diminution in the follicle lumen area. Electron microscopy unveiled pronounced endoplasmic reticulum markedly swelling and expansion within the HDP group. Molecular evaluations revealed a decrement in Tg expression within thyroid tissue, concomitant with an upregulated expression of p-PERK, P-eIF2α, and ATF4.Conclusion: This investigation suggests that HDP might modulate Tg expression within thyroid tissue, possibly mediated through the PERK/eIF2α signaling cascade. This perturbation may compromise thyroid hormone synthesis, thereby predisposing pregnant rats to subclinical hypothyroidism.
研究领域:
内分泌学
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生殖医学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
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2016
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IF 2.187
Zuoliang Xie,Jianping Wang,Fen Dai,Xubing Jin,Kele Wu,Qiong Chen,Yuhuan Wang
This study aimed to investigate the effects of maternal exposure to di- n -butyl phthalate (DBP) during pregnancy and breastfeeding on F1 ovarian development and function. A rat model of maternal exposure to DBP during pregnancy and breastfeeding was established by gavage feeding female Sprague Dawley rats with 0, 10, 100, or 600 mg/kg/day DBP from gestational day (GD) 12 to postnatal day (PND) 21. F1 offspring were weaned on PND21 and were not exposed to DBP afterward. The age of vaginal opening and estrus onset, estrous cyclicity, c-Kit-ligand expression on ovarian granulosa cells, and the weight of ovaries and uterus of F1 female offspring were not affected, whereas serum levels of estradiol and progesterone were increased significantly by maternal exposure to 10 mg/kg/day DBP from GD12 to PND21. Although F1 ovarian function may not be adversely affected by maternal exposure to DBP, the increased reproductive hormone levels may interfere in F1 rat fertility.
研究领域:
生殖毒理学
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内分泌学
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发育生物学
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产品关联
DOI
PubMed
高影响因子
PLACENTA
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2024
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IF 3
Gaoying Wang,Ruirui Dong,Haijian Zhao,Ningzhen Ye,Jing Wang,Jing Cheng,Xinrui Shi,Liang Luo,Ting Zhang
Background Abnormal bile acid metabolism leading to changes in placental function during pregnancy. To determine whether endoplasmic reticulum protein 29 (ERp29) can mediate the pregnancy effects of cholestasis by altering the level of trophoblast cell apoptosis. Methods ERp29 in serum of 66 intrahepatic cholestasis of pregnancy (ICP) pregnant women and 74 healthy were detected by ELISA. Subcutaneous injection of ethinyl estradiol (E2) was used to induce ICP in pregnant rats. Taurocholic acid (TCA) was used to simulate the ICP environment, and TGF-β1 was added to induce the epithelial mesenchymal transformation (EMT) process. The scratch, migration, and invasion test were used to detect the EMT process. ERp29 overexpression/knockdown vector were constructed and transfected to verify the role of ERp29 in the EMT process. Downstream gene was obtained through RNA-seq. Results Compared with the healthy pregnant women, the expression levels of ERp29 in serum of ICP pregnancy women were significantly increased (P < 0.001). ERp29 in the placenta tissue of the ICP pregnant rats increased significantly, and the level of apoptosis increased. The placental tissues of the ICP had high expression of E-cadherin and low expression of N-cadherin, snail1, vimentin. After HTR-8/SVneo cells were induced by TCA, EMT was inhibited, while the ERp29 increased. Cell and animal experiments showed that, knockdown of ERp29 reduced the inhibition of EMT, the ICP progress was alleviated. Overexpression of FOS salvaged the inhibitory effects of ERp29 on cell EMT. Discussion The high level of ERp29 in placental trophoblast cells reduced FOS mRNA levels, inhibited the EMT process and aggravated the occurrence and development of ICP.
研究领域:
妇产科学
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分子生物学
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细胞生物学
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病理学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2022
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IF 16.744
Ying Wen,Zhiqing Long,Xiaoxia Bai,Fangjun Huo,Caixia Yin
The early diagnosis and effective treatment of disease have presented new challenges to today's researchers. Recently, an important direction of the development of the fluorescent probes field is to provide convenient detection tools for clinical diagnosis. Intrahepatic cholestasis of pregnancy (ICP), a pregnancy-specific liver disease associated with poor maternal and fetal outcome, lack biochemical diagnosis indicator with relatively high specificity. Glutathione S -transferases may be a potential candidate for ICP diagnosis owing to their high concentrations in the liver, however, fluorescent detection tool for GSTs with high selectivity is scarce, owing to the interference of high non-enzymatic reaction with GSH. In this work, a probe ( MB5 ) through fine structure regulation for specific fluorescence response GSTs was obtained with synergistic activation of enzymes and position-dependent of electrophilic groups. Furthermore, overexpressed GSTs were visualized directly in cholestatic mice models through in vivo imaging, isolated organ imaging, and tissue slice analysis. More importantly, the serum samples of the ICP patients hold significantly high levels of GSTs concentrations, compared with healthy pregnant women. Therefore, a novel diagnostic tool and excellent detection method for ICP were provided, which will promote to the establishment of new biochemical markers of liver function in pregnancy.
研究领域:
生物医学工程
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分子生物学
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临床诊断
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药理学
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医学成像
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产品关联
DOI
高影响因子
Food Bioscience
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2024
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IF 4.8
Mingxin Li,Shugang Li,Chunqing Ai,Wenxiu Zhu,Donglin Sui,Yixuan Li,Binbin Hou,Xiaomeng Ren
The increasing incidence of atopic dermatitis (AD) presents a public health issue, and thus there is an urgent need for safe and effective preventive strategies. In this study, a strategy to reduce the risk of AD in offspring by administering probiotics to the mother was explored. Female mice were given Lactobacillus rhamnosus RL-H3-005 (RL) and Pediococcus acidilactici RP-H3-006 (RP) during pregnancy and lactation period, and their inhibition effects on AD symptoms in offspring mice were evaluated. The results showed that supplementation with RL and RP during pregnancy and lactation period altered not only the gut microbiota community but also microbial community in milk in pregnant mice. Further study showed that pups from RL- and RP-treated pregnant mice had mild symptoms of AD, characterized by reduced epidermal thickness in the ear tissues and numbers of mast cells, which was associated with the reduction in secretory immunoglobulin A (sIgA), immunoglobulin E (IgE), IL-4, IL-5, IL-13, thymic stromal lymphopoietin (TSLP), and eosinophil cationic protein (Ecp). The underlying mechanism of RL and RP on the inhibition of AD symptom in offspring was closely associated with the alterations of the gut microbiota community in offspring, including an increase in Ligilactobacillus and Bacteroides . This study implies that RL and RP hold the potential to reduce the risk of AD in offspring via gut-mammary axis during the pregnancy and lactation period.
研究领域:
微生物学
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免疫学
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分子生物学
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药理学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）