ANNALS OF MEDICINE
·
2023
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IF 4.4
Zhengwen Xu,Youran Li,Ying Liu,Shengmin Liu,Lin Zhang,Jing Wang,Shaofei Su,Lin Liu,Lanlan Meng,Hongyuan Zhu,Jingru Sun,Lijun Shao,Lin Li,Yanhong Zhai,Guanghui Li,Zheng Cao
Background As folates are essential for embryonic development and growth, it is necessary to accurately determine the levels of folates in plasma and red blood cells (RBCs) for clinical intervention. The aims of this study were to develop and validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantitation of folates in plasma and RBCs and to examine the association between plasma and RBC folate concentrations and gestational diabetes mellitus (GDM), gestational hypertension (GH) and preeclampsia (PE).Methods With the in-house developed LC-MS/MS, a retrospective cross-sectional study was conducted. The healthy pregnant women of first- (n = 147), second- (n = 84) and third-trimester (n = 141) or the women diagnosed with GDM (n = 84), GH (n = 58) or PE (n = 23), that were aged between 22 and 46 years old and registered at our institute, were subjected for measurement of folic acid (FA) and 5-methyltetrahydrofolate (5-MTHF), followed by appropriate statistical association analysis.Results The assay for simultaneous quantitation of FA and 5-MTHF in plasma and RBCs was linear, stable, with imprecision less than 15% and recoveries within ±10%. The lower limits of quantification for FA and 5-MTHF measurement in whole blood were 0.57 and 1.09 nmol/L, and in plasma were 0.5 and 1 nmol/L, respectively. In the association analysis, the patients with lower RBC folate level (<906 nmol/L) presented higher risks of PE development (OR 4.861 [95% CI 1.411–16.505]) by logistic regression and restricted cubic spline (RCS) regression in a nonlinear fashion. In addition, higher level of plasma folates in pregnancy was significantly associated with GH risk but may be protective for the development of GDM.Conclusions The in-house developed LC-MS/MS method for folates and metabolites in plasma or RBC showed satisfactory analytical performance for clinical application. Further, the levels of folates and metabolites were diversely associated with GDM, GH and PE development.
研究领域:
临床医学
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营养学
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分析化学
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DOI
PubMed
高影响因子
American Journal of Translational Research
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2025
·Vol. 17
·期 3
·2005
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IF 1.6
Xiaolan Zhuang,Xingxing Li,Shikai Yu,Lin Zeng,Yuping Xu,Tingting Hu,Sailong Wan
Objective: This study aimed to evaluate the clinical efficacy of labetalol (LBT) combined with magnesium sulfate (MgSO 4 ) in improving symptoms and pregnancy outcomes in patients with hypertensive disorders of pregnancy (HDP). Methods: A total of 122 HDP patients admitted between January 2020 and June 2024 were included. Among them, 56 patients (control group) received MgSO 4 alone, while 66 patients (research group) received a combination of LBT and MgSO 4 . Comparative analyses were conducted to evaluate therapeutic effectiveness, symptom improvement (edema, hypertension, and proteinuria), brain-related symptoms (nausea and vomiting, sluggishness, blurred vision, dizziness, and headache), pregnancy outcomes (placental abruption, neonatal asphyxia, fetal distress, and postpartum hemorrhage), blood pressure indicators (systolic (SBP) and diastolic blood pressure (DBP)), fetal umbilical artery hemodynamics (systolic-to-diastolic flow velocity ratio (S/D) and pulsatility index (PI)), and high-sensitivity C-reactive protein (hs-CRP). Results: The research group demonstrated significantly better outcomes than the control group, including a higher overall therapeutic effectiveness rate, faster resolution of edema, hypertension, and proteinuria, a lower incidence of adverse pregnancy events, and more pronounced reductions in SBP, DBP, S/D, PI, and hs-CRP levels after treatment (all P < 0.05). Conclusion: The combination of LBT and MgSO 4 is highly effective in improving symptoms and pregnancy outcomes in HDP patients, outperforming MgSO 4 monotherapy. This approach has strong potential for broader clinical application.
研究领域:
妇产科学
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药理学
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DOI
PubMed
高影响因子
BMC Pregnancy and Childbirth
·
2023
·Vol. 23
·期 1
·1-10
·
IF 3.1
Ding Ailing,Wan Huimin,Peng Juan,Wang Huizi,Zhu Shaodan,Dong Xudong
Background Hypertensive disorders complicating pregnancy (HDCP) and gestational diabetes mellitus (GDM) can affect the placental barrier function to varying degrees. However, current studies show that the transfer and distribution characteristics of trace elements in the maternal-fetal system are still unclear. This study investigated the effect of the placental barrier on the transfer of trace elements from mother to fetus and its relationship with HDCP and GDM. Methods A case-control method was used in this study. 140 pairs of samples were collected; 60 were from healthy pregnant women, and 80 were from patients with pregnancy complications. The contents of trace elements in paired samples were determined by inductively coupled plasma–mass spectrometry (ICP-MS). SPSS software was used to analyze the differences in trace element levels in matched samples of each group. The correlations were analyzed based on Pearson’s correlation factor (r). Results The distribution characteristics of Fe content in the pathological group (HDCP group and GDM group) were the same as those in the normal group (umbilical cord blood > maternal blood > placenta), but there was no significant difference in the iron content in maternal blood and cord blood of pathological group. The distribution characteristics of Mn content in the pathological group (placenta > umbilical cord blood > maternal blood) were changed compared with those in the normal group (placenta > maternal blood > umbilical cord blood). In addition, the placental Cr content and cord blood Cr and Ni content of the pathological group were higher than those of the normal group. HDCP placental Cr and GDM placental Fe levels were significantly correlated with the Apgar score. Conclusions The transfer of Fe and Mn and the placental barrier function of Cr and Ni in the maternal-fetal system of HDCP and GDM are significantly altered, which directly or indirectly increases the maternal and fetal health risk.
研究领域:
妇产科学
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营养学
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病理学
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DOI
PubMed
高影响因子
Orphanet Journal of Rare Diseases
·
2021
·Vol. 16
·期 1
·1-13
·
IF 4.123
Shao Yong,Chen Siyu,Li Huan,Tang Qin,Xu Di
Background ICP pregnant women have a unique profile of serum bile acid metabolism, thus the early and accurate identification of ICP patients is beneficial to early appropriate treatment and improvement of pregnancy outcomes. In this study, ultra-high performance liquid chromatography-mass spectrometry/mass spectrometry (UPLC-MS/MS) was used to analyze the 15 types of serum bile acid profiles among patients with ICP in third trimester, patients with cholelithiasis, and patients with hepatitis B virus. The ICP diagnostic model established by partial least squares-discriminant analysis (PLS-DA) was used to screen the differential bile acids for clinical subtypes of ICP. 144 cases of ICP patients were involved in this study, and divided into four subgroups according to serum level of TBA, DBIL, and ALT.Results (1) The differential serum bile acid profiles of ICP group and normal pregnant women were DCA, TDCA, TCA, GDCA and GLCA. (2) The differential serum bile acid profiles of the ICP1 group (ICP with jaundice) and normal pregnant women were TCDCA, TCA, GCA, GCDCA, TUDCA and GUDCA. (3) The differential serum bile acid profiles of the ICP3 group (Hyperchoicemia of pregnancy) and normal pregnant group was GUDCA, LCA, GLCA, UDCA, TUDCA, CDCA, and TLCA (P < 0.05). (4) The differential serum bile acid profiles of ICP4 group (idiopathic aminotransferase abnormality during pregnancy) and normal pregnant group was UDCA, GUDCA, TUDCA, GCA and GLCA (P < 0.05). (5) The occurrence of meconium-stained amniotic fluid, premature delivery and cesarean section in ICP1 group was significantly higher than normal group, ICP2 group, ICP3 group, and ICP4 group (P < 0.05); The occurrence of meconium-stained amniotic fluid, premature delivery and cesarean section in ICP2 group, ICP3 group, and ICP4 group was significantly higher than normal group (P < 0.05), but no difference was found among ICP2 group, ICP3 group, and ICP4 group (P > 0.05).Conclusion Maternal serum bile acid profiles are useful to differentiate the four subtypes of ICP. ICP with jaundice could be an important predictor of adverse pregnancy outcomes of ICP.
研究领域:
妇产科学
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代谢组学
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DOI
PubMed
高影响因子
Frontiers in Endocrinology
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2025
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IF 4.6
Congrong Wu,Maomao Sun,Yue He,Jie Jiang,Luran Wang,Yanni Wang,Yonghui Yu
Background: Clinical research has identified a correlation between hypertensive disorders of pregnancy (HDP) and subclinical hypothyroidism during gestation. But the potential influence of HDP on thyroid hormone synthesis remains undetermined.Aims: This study aims to elucidate the impact of HDP on thyroid hormone synthesis and to delineate the underlying mechanisms.Methods: 20 pregnancy SD rats were stratified at random into the HDP group and the Control group. The HDP group was subjected to NG-Nitro-L-arginine-methylester administration from gestational days 13 to 20, while the Control group received saline. Subsequent assessments encompassed serum FT4, FT3, and TSH concentrations, morphological examination of the thyroid, as well as quantification of essential proteins pivotal to thyroid hormone synthesis and markers indicative of endoplasmic reticulum stress.Results: The HDP group exhibited a statistically significant augmentation in serum TSH concentrations (p<0.05), while FT3 and FT4 levels manifested no discernible statistical variations. H&E staining highlighted a pronounced hyperplasia of the follicular epithelial cells and a diminution in the follicle lumen area. Electron microscopy unveiled pronounced endoplasmic reticulum markedly swelling and expansion within the HDP group. Molecular evaluations revealed a decrement in Tg expression within thyroid tissue, concomitant with an upregulated expression of p-PERK, P-eIF2α, and ATF4.Conclusion: This investigation suggests that HDP might modulate Tg expression within thyroid tissue, possibly mediated through the PERK/eIF2α signaling cascade. This perturbation may compromise thyroid hormone synthesis, thereby predisposing pregnant rats to subclinical hypothyroidism.
研究领域:
内分泌学
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生殖医学
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分子生物学
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DOI
PubMed
高影响因子
Lancet Global Health
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2021
·
IF 26.763
Kui Deng,Juan Liang,Yi Mu,Zheng Liu,Yanping Wang,Mingrong Li,Xiaohong Li,Li Dai,Qi Li,Peiran Chen,Yanxia Xie,Jun Zhu,Hanmin Liu
Background Preterm birth rates have increased significantly worldwide over the past decade. Few epidemiological studies on the incidence of preterm birth and temporal trends are available in China. This study used national monitoring data from China's National Maternal Near Miss Surveillance System (NMNMSS) to estimate the rate of preterm birth and trends between 2012 and 2018 in China and to assess risk factors associated with preterm birth. Methods In this observational study, data were sourced from the NMNMSS between Jan 1, 2012, and Dec 31, 2018. Pregnancies with at least one livebirth, with the baby born at 28 weeks of gestation or more or 1000 g or more birthweight were included. We estimated the rates of overall preterm, very preterm (born between 28 and 31 weeks’ gestation), moderate preterm (born between 32 and 33 weeks’ gestation), and late preterm (born between 34 and 36 weeks’ gestation) births in singleton and multiple pregnancies and assessed their trends over time. We used logistic regression analysis to examine the associations between preterm birth and sociodemographic characteristics and obstetric complications, considering the sampling strategy and clustering of births within hospitals. Interrupted time series analysis was used to assess the changes in preterm birth rates during the period of the universal two child policy intervention. Findings From Jan 1, 2012, to Dec 31, 2018, 9 645 646 women gave birth to at least one live baby, of whom 665 244 (6·1%) were born preterm. In all pregnancies, the overall preterm birth rate increased from 5·9% in 2012 to 6·4% in 2018 (8·8% increase; annual rate of increase [ARI] 1·3 [95% CI 0·6 to 2·1]). Late preterm births (8·8%; ARI 1·5% [0·9 to 2·2]) and very preterm births (13·3%; ARI 1·8% [0·5 to 3·0]) significantly increased from 2012 to 2018, whereas moderate preterm births did not (3·8%; ARI 0·3% [95% CI –0·9 to 1·5]). In singleton pregnancies, the overall preterm birth rate showed a small but significant 6·4% increase (ARI 1·0% [0·4 to 1·7]) over the 7 year period. In multiple pregnancies, the overall preterm birth rate significantly increased from 46·8% in 2012 to 52·7% in 2018 (12·4% increase; ARI 1·9% [1·2 to 2·6]). Compared with women who gave birth in 2012, those who gave birth in 2018 were more likely to be older (aged ≥35 years; 7·4% in 2012 vs 15·9% in 2018), have multiples (1·6% vs 1·9%), have seven or more antenatal visits (50·2% vs 70·7%), and have antepartum complications and medical disease (17·9% vs 35·1%), but they were less likely to deliver via caesarean section (47·5% vs 45·0%). Compared with the baseline period (January, 2012 to June, 2016), a higher increase in preterm birth was observed after the universal two child policy came into effect in July, 2016 (β=0·034; p=0·03). Interpretation An increase in preterm births was noted for both singleton and multiple pregnancies between 2012 and 2018 in China. China's strategic investment in maternal and neonatal health has been crucial for the prevention of preterm birth. Due to rapid changes in sociodemographic and obstetric factors related to preterm birth—particularly within the context of the universal two child policy—such as advanced maternal age at delivery, maternal complications, and multiple pregnancies, greater efforts to reduce the burden of preterm birth are urgently needed. Funding National Key R&D Program of China, National Health Commission of the People's Republic of China, China Medical Board, WHO, and UNICEF.
研究领域:
公共卫生
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妇产科学
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流行病学
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妇幼保健
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DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2016
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IF 3.055
Hui-hui Xie,Jian-ya Xu,Tong Xie,Xin Meng,Li-li Lin,Li-li He,Hao Wu,Jin-jun Shan,Shou-chuan Wang
Ethnopharmacological relevance Banxia (BX) is the root of Pinellia ternata (Thunb.) Berit. Its processed products, such as Jiang Banxia (JBX), have been clinically used in traditional Chinese medicine to treat vomiting, coughing , and inflammation. However, data for their safety for pregnant women are contradictory and confusing. Aim of the study To further explore the safety of BX, an ultra-performance liquid chromatography coupled with liquid chromatography–mass spectrometry (LC–MS) and gas chromatography-mass spectrometry (GC–MS) metabolomics approach was used to evaluate the metabolic perturbation in pregnant rats caused by BX and JBX. Materials and methods Placenta and amniotic fluid samples were collected from control Sprague-Dawley pregnant rats and exposed to BX suspension and JBX decoction (1.434 g/kg/day). Samples were analyzed using LC–MS and GC–MS. The acquired MS data of above samples were further subjected to multivariate data analysis, and the significantly altered metabolites were identified. The associated pathways were constructed using MetaboAnalyst 3.0. Results The weight and histopathology of the placenta from each group of rats had no definite difference. However, we found 20 differential endogenous metabolites that changed significantly in the placenta and amniotic fluid samples. The alterations of identified metabolites indicated a perturbation in glycerophospholipid metabolism, amino acid metabolism, and carbohydrate metabolism in pregnant rats exposed to BX and JBX. Conclusion In summary, this work suggested that oral administration of BX and JBX may induce disturbances in the intermediary metabolism in pregnant rats. This work contributes to further understanding the safety of BX and its processed products.
研究领域:
药理学
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传统医学
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代谢组学
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DOI
高影响因子
Food Frontiers
·
2021
·Vol. 2
·期 2
·208-217
·
IF 0
Huiting Lin,Jiawen Zhang,Shiyang Li,Baodong Zheng,Jiamiao Hu
The current study was aimed to explore the beneficial effects of p olysaccharides isolated from Laminaria japonica (LP) on gestational diabetes mellitus (GDM) mice. The obtained results demonstrated that the LP improved serum biochemical index, body weight index, and glucose tolerance. Furthermore, the evidence also suggested that the beneficial effects of LP might be attributed to the alternation in gut microbiota by LP supplementation. Particularly, the Turicibacter , a short-chain fatty acids-producing bacterium, was found to be up-regulated. In conclusion, the obtained results indicated that the LP might serve as prebiotic, and might have a great potential to be used as food supplementation for GDM patients.
研究领域:
营养学
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微生物学
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医学
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DOI
Food & Function
·
2020
·Vol. 11
·期 9
·8089-8098
·
IF 4.171
Liuqin He,Yonghui Liu,Jing Long,Xihong Zhou,Sijing Zeng,Tiejun Li,Yulong Yin
Maternal dietary serine affects free amino acid content in milk and the antioxidant ability of progeny. However, whether maternal dietary serine has any effects on offspring performance in pigs and related metabolic consequences remains unknown. This study was conducted to investigate the effects of different levels of maternal dietary serine from late pregnancy to lactation on sow reproductive performance and offspring performance, and on the metabolome of milk and the serum of sows and their offspring. The results showed that sows fed a diet supplemented with 25% serine of the basal diet (L-Ser) had a higher litter weight, and higher average piglet weight at birth and aged 21 days when compared with sows fed the basal diet (CON). We found a large number of metabolites in both colostrum and milk that differed significantly between sows in the CON and L-Ser groups. Additionally, twenty metabolites differed in the serum of piglets aged 21 days between the CON and L-Ser groups. Most of these metabolites are involved in purine and pyrimidine metabolism, glutathione and taurine metabolism, as well as phospholipid and sphingolipid metabolism, which may contribute to the growth-promoting effects of serine on offspring. Our results imply that maternal serine has the potential to improve offspring outcomes.
研究领域:
动物营养学
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代谢生物学
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生殖生理学
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产品关联
DOI
PubMed
高影响因子
Animals
·
2020
·Vol. 10
·期 2
·251
·
IF 2.323
Jie Peng,Mao Xia,Jia Xiong,Chenbin Cui,Ningning Huang,Yuanfei Zhou,Hongkui Wei,Jian Peng
Simple SummaryHydrogen sulfide (H2S) is an important second messenger, which has been implicated in regulating placental angiogenesis. Our findings revealed that gestational dietary methionine could affect maternal and placental H2S concentrations. With the increase of dietary methionine, maternal plasma and placental H2S concentrations changed quadratically, which was consistent with placental vascular density and reproductive performance. The decrease in H2S production caused by an increase in dietary methionine was likely to be the cause for the increase in the rate of low birth weight piglets and needs further study.AbstractThe placenta is a unique bond between the mother and the fetus during pregnancy, and a proper placental angiogenesis is vital for fetal development. H2S is an endogenous stimulator of angiogenesis that is mainly produced by the methionine transsulfurationpathway. The goal of this study was to evaluate the effect of gestational dietary methionine on maternal and placental H2S production in sows. Multiparous sows (Large×White; third parity;n= 65) were randomly allocated into five groups, with feed diets comprisingstandardized ileal digestible methionine/lysine (Met/Lys) ratios of 0.27 (nutrient requirements of swine (NRC); 2012 level), 0.32, 0.37, 0.42, and 0.47, respectively. The litter size and weight at birth were measured and recorded. Maternal blood samples were obtained at embryonic day (E) E40 d, E90 d, and E114 d of gestation. The placental samples were collected at parturition. The results showed that maternal plasma H2S concentration was not affected at E40 d. However, the maternal plasma H2S concentration changed quadratically with the dietary Met/Lys ratio at E90 d (p< 0.01) and E114 d (p= 0.03). The maximum maternal plasma H2S concentration was at the dietary Met/Lys ratio of 0.37. Meanwhile, maternal plasma H2S concentration was positively correlated with piglets born alive (p< 0.01) and litter weight (p< 0.01). Consistent with the maternal plasma, the placental H2S concentration also changed quadratically with the dietary Met/Lys ratio (p= 0.03); the Met/Lys ratio of 0.37 showed the maximum H2S concentration. In conclusion, our findings revealed that the gestational dietary Met/Lys ratio could affect maternal and placental H2S concentrations, which may be an important molecular mechanism affecting placental angiogenesis and piglet development.Keywords:hydrogen sulfide;reproductive performance;pregnant sows;methionine;placental angiogenesis
研究领域:
动物营养学
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生殖生物学
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DOI
PubMed
高影响因子
Frontiers in Endocrinology
·
2021
·
IF 5.555
Bai Yu,Du Qiang,Zhang Le,Li Ling,Wang Nana,Wu Bo,Li Ping,Li Ling
This study aims to investigate the effect of angiopoietin like 8 (ANGPTL8) on gestational diabetes mellitus (GDM) and insulin resistance (IR). The GDM model was induced by high fat diet in mice, and IR was observed. The expression and secretion of ANGPTL8 were promoted in placenta of GDM mice. IR was induced in trophoblast cell HTR-8/SVneo by treatment of high concentration of insulin, and the expression levels of ANGPTL8 were increased. Silencing of ANGPTL8 alleviated IR and decreased glucose uptake in HTR-8/SVneo cells. However, the inflammation and oxidative stress in IR cells were not restrained by ANGPTL8 knockdown. In addition, c-Jun N-terminal kinase (JNK) signaling was activated by IR, which was inhibited by silencing of ANGPTL8. The effect of ANGPTL8 knockdown on IR was attenuated by JNK antagonist, and aggravated by JNK agonist, suggesting that ANGPTL8 affected IR by regulating JNK signaling. In conclusion, we demonstrated that the silencing of ANGPTL8 ameliorated IR by inhibiting JNK signaling in trophoblast cells. These findings may provide novel insights for diagnosis and treatment of GDM in clinic.
研究领域:
内分泌学
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妇产科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ChemistryOpen
·
2024
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IF 2.5
Lishan Fang,Jinqiu Li,Wei Lin,Lili Zeng,Liumin Yu,Zhanfei Chen,Jianlin Shen,Yu Chen,Zhonghui Chen,Zhenyu Lin
Graphical A hydrogen sulfide (H 2 S) stimuli-responsive hydrogel was constructed based on the coordination and bonding principle of metal ions and chitosan (CS). The hydrogel can be rapidly prepared within 5 s. In the presence of H 2 S, the cross-linking agent Cu 2+ can compete out, and release of the electrochemical probe. The prepared hydrogel has a good linear relationship with H 2 S. Oxidative stress caused by pregnancy-induced hypertension syndrome significantly affects the health of pregnant women. Hydrogen sulfide is a typical gaseous signal molecule against oxidative stress, and it is of profound significance to develop a detection method. In this study, a stimuli-responsive hydrogel was constructed based on the coordination and bonding principle of metal ions and chitosan (CS) to realize the quantitative detection of hydrogen sulfide (H 2 S). The chain of CS contains a large number of amino groups and hydroxyl groups, which can form the coordination structure with Cu 2+ , triggering CS to form a stable hydrogel. The hydrogel can be formed within about 5 s, which has the characteristics of rapid preparation. In the presence of target H 2 S, the cross-linking agent Cu 2+ in the hydrogel can compete out, resulting in the collapse of the hydrogel and the release of the electrochemical probe. By detecting the concentration of the released electrochemical probe, the quantitative detection of H 2 S can be achieved. The prepared hydrogel has a good linear relationship with the concentration of H 2 S from 1 μM to 60 μm. At the same time, the hydrogel has good specificity and stability, and it can be applied to the detection of H 2 S in serum samples.
研究领域:
生物医学工程
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分析化学
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产品关联
DOI
PubMed
高影响因子
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