SCIENCE OF THE TOTAL ENVIRONMENT
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2022
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IF 10.753
Lu Zhang,Tian Xia,Qi Liu,Lei Gu,Yunfei Sun,Zhou Yang
Nitrogenous pollutants derived from human activities not only pose direct risk on aquatic organisms but may also indirectly endanger the stability of interspecific relations. To date, the effects of the nitrogen-containing pollutants on the induced defense remain unclear. Here, we aim to investigate the induced defense of an aquatic keystone species , Daphnia pulex, which responds to predation risk under nitrite pollution at environmentally relevant concentrations and simultaneously evaluate the effects of their induced defenses on nitrite tolerance. Results showed that increasing nitrite significantly reduced the survival time of D. pulex and posed severe reproductive toxicity, consequently reducing the offspring and broods. In the morphological defensive responses, early nitrite exposure interfered with the spine elongation, but the relative spine length induced by the predation risk was unaffected by the nitrite concentrations with exposure time prolonged, although high-dose nitrite inhibited the spine elongation and the increase of the body size. The integration of biomarker response index analyses further indicated that the reproductive capacity was more seriously impaired than the morphology and the survival. Moreover, the sensitivity analyses of growth and reproduction indicated that predation risk significantly reduced Daphnia's tolerance to nitrite. Conclusively, these findings highlight that long-term nitrite exposure exacerbates the predator-induced miniaturization of zooplanktons, and predation risk also reduces their tolerance to nitrite, which provides new insights into the performance changes of zooplanktons exposed to pollutants under predation risk and the vulnerability of predator–prey interspecific relationships in polluted environments.
研究领域:
生态毒理学
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环境科学
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DOI
PubMed
高影响因子
Neuropsychiatric Disease and Treatment
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2024
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IF 2.5
Yueyuan Wang,Bo Lv,Kai Fan,Cunjin Su,Delai Xu,Jie Pan
Purpose Parkinson’s disease (PD) is a common neurodegenerative disease that severely affects patients’ daily lives and places a significant burden on the global economy. There are currently no specific biomarkers for distinguishing between the different stages of PD.Methods We divided 78 mice into six equal groups, including five model PD groups (W1–W5; based on the PD stage induced by length of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine/propofol induction time) and a control group. Then, we used metabolomics technology to detect the serum small-molecule metabolites present in each group. Ultimately, we screened for potential biomarkers using the variable importance in the projection of the orthogonal partial least squares discriminant analysis and the coefficient value of LASSO ordinal logistic regression.Results We identified 12 potential biomarkers, including dehydroepiandrosterone sulfate, pipecolic acid, N-acetylleucine, 2-aminoadipic acid, L-tyrosine, uric acid, and 5-hydroxyindoleacetaldehyde. Pathway analysis revealed their involvement in amino acid metabolism, caffeine metabolism, steroid hormone biosynthesis, and purine metabolism. Additionally, the receiver operating characteristic curve indicated that a biomarker panel comprising the 12 biomarkers could differentiate between the different PD stages.Conclusion Different PD stages are characterized by different metabolites. The biomarkers identified in this study are helpful to understand the PD process.
研究领域:
神经科学
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代谢组学
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医学
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2024
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IF 6.2
Cuiyuan Jin,Caiyun Wang,Chen Zhang,Qi Ye,Yuanxiang Jin,Liyun Shi
As a fungicide with the characteristics of high effectiveness, internal absorption and broad spectrum, imazalil is widely used to prevent and treat in fruits and vegetables. Here, pregnant C57BL/6 mice were exposed to imazalil at dietary levels of 0, 0.025‰, and 0.25‰ through drinking water during pregnancy and lactation. We then analyzed the phenotype, metabolome, and expression of related genes and proteins in the livers of mice. There was a marked decrease in the body and liver weights of male offspring mice after maternal imazalil exposure, while this effect on the dam and female offspring was slight. Metabolomics analyses revealed that imazalil significantly altered the metabolite composition of liver samples from both dams and offspring. The preliminary results of the analysis indicated that glucolipid metabolism was the pathway most significantly affected by imazalil. We performed a coabundance association analysis of metabolites with significant changes in the pathway of glycolipid metabolism, and IMZ altered the networks of both dams and offspring compared with the network in control mice, especially in male offspring. The hepatic triglyceride, non-esterified fatty acid and glucose levels were increased significantly in the dams but decreased significantly in male offspring after maternal imazalil exposure. Furthermore, the expression levels of genes associated with glycolipid metabolism and m 6 A RNA methylation were significantly affected by maternal intake of imazalil. Imazalil-induced glucolipid metabolism disturbance was highly correlated with m 6 A RNA methylation. In conclusion, maternal imazalil exposure resulted in glucolipid metabolism disturbance and abnormal m 6 A RNA methylation in the livers of dams and offspring mice. We expected that the information acquired in this study will provide novel evidence for understanding the effect of maternal imazalil exposure on potential health risks.
研究领域:
毒理学
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代谢组学
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分子生物学
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发育生物学
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
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IF 6
Cong Li,Zhijie Zheng,Guiying Wang,Guanghui Chen,Nannan Zhou,Ruwei Ren,Qiongfang Yang,Wenxi Fu,Bo Li,Guozhou Liao
To elucidate the impact of microorganisms on the metabolites and flavor of Xuanwei ham, the correlation between volatile compounds, small molecule metabolites, and microbial communities in Xuanwei ham was studied. The results showed that the predominant bacteria and fungi were Proteobacteria , Firmicutes , and Ascomycota , respectively at the phylum level, and the main bacteria was Staphylococcus , and the predominant fungi was Aspergillus at the genus level. A total of 48 differential metabolites were screened, and amino acids were the most abundant. Upon pathway analysis, 21 metabolic pathways were found to be related to ham ripening. More than 20 key metabolites such as arginine were accurately matched by Public Chemistry Database (Pub Chem) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Eighteen volatile flavor characteristic compounds were identified, and the relative content of acetone in ham samples from various years was the highest. Additionally, the findings explored the relationship between small molecule metabolites, volatile compounds, and microorganisms in Xuanwei ham, which showed that the microorganisms in the samples had a positive effect on a variety of amino acids and alcohols. The findings represent a fertile ground for the formation of ham quality.
研究领域:
食品科学
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微生物学
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代谢组学
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DOI
高影响因子
ACS Applied Materials & Interfaces
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2022
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IF 10.383
Shuai Liu,Mo Zhang,Zhizhen Lai,Hongtao Tian,Yuming Qiu,Zhili Li
To date, advanced chemical biology tools for chemoselective extraction of metabolites are limited. In this study, unique coral-like polymer particles were synthesized via high concentrations of 1-ethyl-3-(3-(dimethylamino)propyl) carbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS), which are usually used as condensation agents. The polymers can wrap or adhere Fe3O4 nanoparticles (Fe3O4-NPs) to form polymer magnetic microparticles (PMMPs). With abundant NHS-activated moieties on their surface, the coral-like PMMPs could be modified by cystamine for the chemoselective extraction of phosphate/carboxylate anion metabolites from complex biological samples. Finally, 97 metabolites including nucleotides, phosphates, phosphate sugars, carboxylate sugars, and organic acids were extracted and identified from serum, tissues, and cells. These metabolites are involved in four major metabolic pathways including glycolysis, the tricarboxylic acid cycle, the pentose phosphate pathway, and nucleotide metabolism. This study has provided a cost-effective and easy-to-implement preparation of PMMPs with a robust chemoselective extraction ability and versatile applications.
研究领域:
生物化学
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分析化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Journal of Radiation Research and Applied Sciences
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2024
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IF 1.7
Siqinbilige Wu,Jingxian Gao,Yinzhabu Bai,Zhen Bai,Changxi Bai
Introduction Human constitution is closely linked to health and disease and serves as a basis for disease diagnosis. In this work, the urinary metabolomics of three basic traditional Mongolian medicine constitution (MMC) types were analyzed using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) and nuclear magnetic resonance (NMR) techniques. Materials and methods 90 volunteers majoring in Mongolian medicine from a university during 2021.1–2023.1 were averagely grouped based on the characteristics of MMC using the Classification and Scoring Scale for Mongolian Medicine Constitution : Khii group, Shar group, and Badgan group. NMR technology was employed to determine the urinary metabolites of subjects in different groups. UHPLC-QTOF-MS technology was applied to identify differential metabolites in urine. Multivariate statistical analysis (MSA) including principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and orthogonal partial least squares-discriminant analysis (OPLS-DA) were adopted to analyze the impacts of urinary metabolites. Results the positive ion mode exhibited higher signal intensity with better separation efficiency. Accuracy of UHPLC-QTOF-MS and NMR in Mongolian urinary metabolites exceeded 99%. PCA demonstrated satisfactory clustering analysis results. Covered metabolic pathways encompassed tricarboxylic acid cycle (TCA), fatty acid beta-oxidation, as well as protein, nucleic acid, and amino acid metabolisms. Conclusion the three basic Mongolian constitutions may involve several small molecular metabolites (SMMs) like bile acids, carnitine, aromatic acids, amino acids, and small molecular carboxylic acids. When clinically diagnosing and treating diseases based on Mongolian constitution, constitutional factors should be considered.
研究领域:
医学
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代谢组学
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传统医学
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产品关联
DOI
高影响因子
JOURNAL OF NUTRITION
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2025
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IF 3.8
Lemei Li,Jinyang Wu,Huilei Hao,Ying Wang,Shaoying Hou
Background Gestational diabetes mellitus (GDM) is prone to induce hepatic injury in offspring. Sialic acid (SA) exhibits hepatoprotective effects, while its potential against GDM-induced liver injury in offspring remains unclear. Objective The present study aimed to investigate the hepatoprotective effects of maternal sialic acid (SA) supplementation in GDM-exposed offspring and to explore its underlying mechanisms. Methods C57BL/6J mice were divided into three groups: a control (NC-m) group, a gestational diabetes mellitus (GDM-m) group, and a gestational diabetes mellitus plus sialic acid (GDM+SA-m) group. The GDM-m group was induced using high-fat diet combined with intraperitoneal streptozotocin (STZ) injection. The GDM+SA-m group was administered N-glycolylneuraminic acid (Neu5Ac), human milk SA, via intragastric gavage at a dose of 50 mg/kg during pregnancy and lactation. Offsprings from three groups were fed a control diet until 8 weeks of age, with 20, 20, and 19 in the NC-o, GDM-o, and GDM+SA-o groups, respectively. Liver was obtained and analyzed for HE staining, AST and ALT, oxidative stress markers, inflammatory cytokines, and non-targeted metabolomics. Results and Conclusion s: Compared with the NC group, GDM significantly elevated liver transaminase levels and increased inflammatory and oxidative stress in offspring mice, with more pronounced effects observed in males. SA intervention partially attenuated this damage. Concurrently, metabolomic analysis revealed that SA significantly regulated lipid metabolism and taurine/hypotaurine pathways. The results suggested that maternal SA intervention ameliorated GDM-induced offspring liver injury potentially by regulating hepatic lipid and taurine/hypotaurine metabolism, providing a novel mechanistic insight for early-life intervention in GDM.
研究领域:
生殖医学
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发育生物学
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肝脏病学
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代谢组学
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围产期医学
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分子营养学
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氧化应激
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2023
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IF 6.2
Shuai Liu,Zhizhen Lai,Mo Zhang,Hongtao Tian,Jiang Zhou,Zhili Li
The analysis of biological samples is often affected by the background matrix. Proper sample preparation is a critical step in the analytical procedure for complex samples. In this study, a simple and efficient enrichment strategy based on Amino-functionalized Polymer-Magnetic MicroParticles (NH 2 -PMMPs) with coral-like porous structures was developed to enable the detection of 320 anionic metabolites, providing detailed coverage of phosphorylation metabolism. Among them, 102 polar phosphate metabolites including nucleotides, cyclic nucleotides, sugar nucleotides, phosphate sugars , and phosphates, were enriched and identified from serum, tissues, and cells. Furthermore, the detection of 34 previously unknown polar phosphate metabolites in serum samples demonstrates the advantages of this efficient enrichment method for mass spectrometric analysis. The limit of detections (LODs) were between 0.02 and 4 nmol/L for most anionic metabolites and its high sensitivity enabled the detection of 36 polar anion metabolites from 10 cell equivalent samples. This study has provided a promising tool for the efficient enrichment and analysis of anionic metabolites in biological samples with high sensitivity and broad coverage, facilitating the knowledge of the phosphorylation processes of life.
研究领域:
分析化学
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生物化学
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医学
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DOI
PubMed
高影响因子
PLoS One
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2023
·Vol. 18
·期 4
·e0283105
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IF 3.7
Guifang Yuan,Song Wen,Xuemei Zhong,Xiaotong Yang,Linrui Xie,Xiuli Wu,Xiaoyu Li
Technically, symptom of offspring asthma is also closely reliant on its maternal high-fiber diet as well as the intestinal microbiome. Fruits and vegetables are abundant in inulin, and this naturally soluble dietary fiber is endowed with a potential value on offspring asthma control through the maternal intake, but the mechanism now remains less studied. In this study, rats were given with inulin-included drinking water, whereas in normal group rats were allowed with normal water. Afterwards, we analyzed both the formations of the offspring intestinal microbiome ahead of asthma model establishment and of the maternal intestinal microbiome through high throughput sequence and the short-chain fatty acids (SCFAs) by metabolomic analysis. Subsequently, lung inflammation indexes were detected by Elisa, and the expression of short-chain fatty acid receptors (GPR41, GPR43) in the offspring of asthma models were evaluated through qPCR assay. Inulin intake resulted in altered maternal intestinal microbiome composition, with a significant increase in SCFAs-producing bacteria (mainly Bifidobacterium ), attenuating the asthmatic inflammatory response in the offspring. Meanwhile, inulin intake during pregnancy modulates the composition of the intestinal microbiome of the offspring, and this alteration appears before the onset of asthma, hence, there should be further studies onto the impacts of offspring’s intestinal microbiome on asthma procession.
研究领域:
免疫学
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微生物学
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营养学
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药理学
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产品关联
DOI
PubMed
高影响因子
Communications Biology
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2025
·Vol. 8
·期 1
·1-17
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IF 5.1
Wang He,Kang Tianyang,Li Weiwei
Gestational diabetes during pregnancy is associated with an increased risk of developmental abnormalities in offspring, but the underlying mechanisms remain unclear. It is not known how maternal metabolism and gut microbes influence kidney development in the fetus. Here we show that gestational diabetes alters maternal gut microbiota and reduces the production of key fatty acids that normally support kidney development in offspring. We find that these changes impair a molecular pathway involving the receptor GPR43 , which promotes the growth and migration of kidney cells. In a mouse model, restoring short-chain fatty acids or transferring gut bacteria from healthy donors improves kidney development in offspring, while blocking GPR43 reverses this effect. This study reveals a previously unknown link between maternal gut metabolism and fetal kidney formation and may guide future strategies to prevent congenital kidney disorders in children born to mothers with gestational diabetes.
研究领域:
发育生物学
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微生物学
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代谢学
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分子生物学
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肾脏病学
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妇产科学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2022
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IF 10.383
Yixuan Huang,Rumin Fu,Zurong Zhu,Chengli Liu,Senwei Liu,Peng Yu,Ling Yan,Zhengnan Zhou,Chengyun Ning,Zhengao Wang
Conductive polymers are promising electrode candidates in the nonenzymatic catalytic detection of small molecule metabolites, due to the tunable electronic conductivity and versatile modifiability. However, the complex catalytic reaction pathway of conductive polymers results in lower detection sensitivity and a narrower linear range compared with clinical metal-based and carbon-based electrodes. Localized surface plasmon resonance (LSPR), characterized by deep strong light-matter coupling, has great potential in driving surface catalytic reactions at an ultrafast rate. Here, we constructed a salix argyracea-like polypyrrole nanowires/silver nanoparticles (PPy/AgNPs) heterojunction electrode using polydopamine as a dopant and chelator. Through cyclic voltammetry, the Mott–Schottky curve, and COMSOL simulation, we demonstrated that the LSPR-excited photocarriers enhanced PPy/AgNPs electrode electrocatalysis. Thus, the detection current response and linear range were significantly improved under the LSPR excitation when taking glucose and hydrogen peroxide as models of small molecule metabolites. Furthermore, we discussed the LSPR-enhanced detection mechanism of PPy/AgNPs electrode from the aspects of the Tafel slope, the apparent electron diffusion coefficient, and the charge transfer resistance. This strategy opens a new avenue toward the design of LSPR-enhanced conductive polymer electrodes.
研究领域:
生物医学工程
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分析化学
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材料科学
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DOI
高影响因子
JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY
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2018
·Vol. 56
·期 13
·1446-1456
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IF 2.588
Peng-Yun Li,Chen He,Wei-Xiang Xie,Hui-Juan Li,Abdul Haleem,Chuan-Shan Hu,Xi-Chuan Li,Wei-Dong He
Block copolymers composed of acrylic acid and methyl methacrylate with three topologies of double linear blocks, poly(acrylic acid) (PAA) linear block/poly(methyl methacrylate) (PMMA) G1-dendron and PAA linear block/PMMA G2-dendron have been prepared by the combination of atom transfer radical polymerization and azide–alkyne click reaction. Proton nuclear magnetic resonance spectroscopy, Fourier transform infrared spectroscopy, and gel permeation chromatography have been adopted thoroughly to identify the chemical structure of those block copolymers with expected topologies. The self-assembly of those block copolymers in the selective solvent has been performed through two mixing routes of gentle and abrupt variation in solvent selectivity, and the morphology of the obtained self-assemblies/aggregates was observed by transmission electron microscopy. Because the abrupt variation route altered sharply the solvent quality during the mixing, the intermolecular association of polymer chains resulted in the smaller self-assemblies but the further growth of smaller self-assemblies was not observed. On the contrary, the gentle variation route changed gradually the solvent quality during the mixing, favoring not only the intermolecular association but also the further growth of self-assemblies to result in larger aggregates. The final morphology of those assemblies/aggregates also exhibited the dependence of PMMA dendron generation. © 2018 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2018 , 56 , 1446–1456
研究领域:
高分子化学
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材料科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）