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FOOD CHEMISTRY · 2011 · IF 3.458

Comparison of sterol composition between Tuber fermentation mycelia and natural fruiting bodies

Yang Tang,Hong-Mei Li,Ya-Jie Tang

Truffle, belonging to Tuber genera, is a nutritious and sterol-rich edible fungus, and sterol is a potential health beneficial compound. A comparison of Tuber sterol composition indicates that the total sterol contents in the fermentation mycelia (i.e., 10.5   mg   g −1 ) ( n   =   3) were approximately 3.2–5.6 times higher than that of the fruiting bodies ( p   <   0.05) with the addition of soybean flour into the basal fermentation media. Moreover, the phytosterol profile of fermentation mycelia could be significantly improved by adding soybean flour into the fermentation media. After the addition of soybean flour, stigmasterol and β-sitosterol appeared in the fermentation mycelia and the contents of total phytosterols (2279   μg   g −1 ) ( n   =   3), brassicasterol (943   μg   g −1 ) ( n   =   3), and campesterol (418   μg   g −1 ) ( n   =   3) were all increased significantly ( p   <   0.05). Moreover, the total contents of sterols and phytosterols in the fermentation mycelia cultured in the soybean media were much higher than those in the fruiting bodies (i.e., 1883–3240 and 479–1832   μg   g −1 , respectively) ( n   =   3, p   <   0.05). This work confirms the potentiality of Tuber fermentation mycelia as the alternative resource for its fruiting bodies from the viewpoint of sterols production.

研究领域: 真菌学 · 营养学 · 食品科学
✓ 已验证 产品关联 DOI PubMed 高影响因子
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MOLECULES · 2015 ·Vol. 20 ·期 4 ·5680-5697 · IF 2.416

Chemical Compositions and Antioxidant Activities of Polysaccharides from the Sporophores and Cultured Products of Armillaria mellea

Shanshan Zhang,Xiaoqian Liu,Lihua Yan,Qiwei Zhang,Jingjing Zhu,Na Huang,Zhimin Wang

Armillaria melleais a traditional Chinese medicinal and edible mushroom. Many cultured products ofA. melleahave been used to develop commercial medicines in recent years. The chemical composition and activities of the major bioactive chemical components—polysaccharides—may be different because of differences in the raw materials used. Four polysaccharides (SP, CMP, CFBP and CFMP) were obtained from wild sporophores and cultured products (including mycelia, fermentation broth and fermentation mixture) ofA. mellea. Their yields, carbohydrate contents, monosaccharide compositions, FT-IR spectra, NMR spectroscopy and antioxidant activities were investigated. All of the polysaccharides were composed of xylose, glucose and galactose without protein. Glucose was the dominant monosaccharide in SP, CMP and CFMP, whereas galactose was the dominant monosaccharide in CFBP. SP and CMP showed higher scavenging DPPH•and ABTS•+activities and reducing power among four polysaccharides. The carbohydrate content and corresponding glucose percentage were positive influences on the antioxidant activities, whereas the corresponding xylose and galactose percentage were negative influences.A. melleapolysaccharides are potential natural antioxidants. Polysaccharides from cultured products, especially mycelia, are good substitutes for SP and are also potential sources for both dietary supplements and food industries.Keywords:Armillaria mellea;polysaccharides;wild sporophores;cultured products;chemical composition;antioxidant activity

研究领域: 微生物学 · 食品科学 · 药理学
✓ 已验证 DOI PubMed 高影响因子
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY · 2024 · IF 5.7

From White to Reddish-Brown: The Anthocyanin Journey in Stropharia rugosoannulata Driven by Auxin and Genetic Regulators

Mei Wang,Tingting Song,Qunli Jin,Zuofa Zhang,Yingyue Shen,Guoying Lv,Lijun Fan,Weilin Feng,Yingmin Qu,Mengyu Wang,Meng Shen,Heqiang Lou,Weiming Cai

Stropharia rugosoannulata, or wine-cap Stropharia, is a well-known edible mushroom cultivated globally. The pileipellis color is a crucial quality attribute of S. rugosoannulata, exhibiting significant variation throughout its developmental stages. However, the pigment types and regulatory mechanisms behind color variation remain unclear. The metabolome analysis found that the anthocyanin biosynthesis pathway was significantly enriched and anthocyanins accumulated steadily in fruiting bodies during three developmental stages. The pileipellis pigment was extracted, and HPLC-MS confirmed the presence of anthocyanins. Notably, significant differences in anthocyanin content were observed among the various colored varieties. Thus, anthocyanins contribute to the pileipellis color of S. rugosoannulata. Through further investigation, this study elucidated, for the first time, the relationship between the “SrNFYA-SrDRF2” regulatory module and anthocyanin accumulation. Combined multiomics assays and HPLC analysis revealed that auxin functions as a signaling molecule that regulates the accumulation of anthocyanins in the pileipellis. Subsequently, the hub gene of anthranilate synthase for auxin synthesis was identified as SrTRP1, and the transcription factor SrMYB1 was verified as a regulator of SrTRP1, influencing auxin accumulation. These findings provide a valuable resource for the targeted enhancement of the quality of S. rugosoannulata.

研究领域: 真菌学 · 植物生物化学 · 基因组学 · 分子生物学
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LWT-FOOD SCIENCE AND TECHNOLOGY · 2025 · IF 6.6

Biofortification of Pleurotus geesteranus via exogenous selenium species: Selenium distribution, speciation and its effect on mineral elements accumulation

Lingyang Yao,Huan Tian,Zhengyu Bao,Shuyun Xie,Tao Feng,Min Sun,Hui Ma,Lingyun Yao,Qiangjun Lang

In this study, four Se species including selenite [Se (VI)], selenate [Se (VI)], potassium,2-selenocyanatoacetate (PSeCA), and selenium ore powder (SeOP) were used for biofortification of Pleurotus geesteranus to obtain Se-enriched mushroom. Based on their effects on mycelium growth of P. geesteranus Jinxiu, [Se (VI)] in the concentration range of 100–1000 μg/kg and other three Se species in the range of 100–2000 μg/kg were adopted in spawn running and fruiting body growth stage, with their effects on production yield and elements accumulation evaluated. The results revealed that PSeCA treatment (200 μg/kg) had the highest fruiting body production (290.61 g/bag), followed by 2000 μg/kg of SeOP treatment (286.24 g/bag). SeMet revealed to be dominant species detected in mushroom, with [Se (VI)] exhibited the highest biological efficiency among the four Se species, and 2000 μg/kg of PSeCA treatment exhibited the highest Se content (10.25 mg/kg) in terminal phase of fruiting body. The results suggested that high concentration of PSeCA would be more favored for the Se biofortification. Moreover, Se treatment could greatly alter the mineral elements content of fruiting bodies, both synergistic and antagonistic relationships on accumulation of mineral elements were observed for each tested Se species.

研究领域: 真菌学 · 农业生物技术 · 环境科学 · 营养科学
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