BIOCHEMICAL ENGINEERING JOURNAL
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2024
·
IF 3.7
Yuan-Zhi Tan,Liang-Zhi Qiao,Shan-Shan Wang,Juan Zhang,Jing Qian,Min Zhu,Shan-Jing Yao,Dong-Qiang Lin
The enormous potential of mRNA-based therapeutics in vaccines and medicine has led to the consideration of various mRNA sizes, ranging from ∼1000 to 10,000 nucleotides (nt). However, the commonly used Oligo-deoxythymidine (Oligo dT) affinity resins are not initially designed for such a wide range of sizes, and present challenges in achieving optimal performance for the separation of a given mRNA. In this paper, two factors of the resin beads, pore size and grafting were focused. The effects of varying pore sizes, ranging from 150 to 850 nm, on both static and dynamic adsorption for varying-length mRNA (1000–8500 nt) were investigated. It was found that static adsorption capacity and uptake kinetics were correlated with the mRNA length. Interestingly, dynamic binding capacities (DBCs) showed an increase-then-decrease trend with increasing pore size. An optimal pore size of about 350 nm could lead to the highest DBCs for all tested mRNA. Additionally, the effects of polymer grafting on the adsorption performance were studied. The results revealed that polymer-grafted resin exhibited 24–55% higher DBCs than non-grafted resin. The enhanced DBCs could be attributed to the improved uptake rate facilitated by the polymer grafted. These investigations emphasize the importance of optimizing pore size and grafting for Oligo dT affinity resins, providing valuable guidance for selecting and designing new resins for different mRNA products.
研究领域:
生物技术
·
分子生物学
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DOI
高影响因子
NANOTECHNOLOGY
·
2022
·Vol. 33
·期 19
·195601
·
IF 3.953
Zhongming Jiang,Jiamin Zhang,Kaizhao Wang,Tianyou Chen,Jiuwei Zhao,Kaijun Wang,Jin Hu
A mild strategy for fabricating nanoporous silver (np-Ag) pieces was reported via preparation of Ag–Ga alloys in relatively low temperature and subsequent electrochemical dealloying in nitric acid (HNO3) aqueous solution. After selectively etching Ga out of the Ag–Ga alloy, a typical three-dimensional (3D) bicontinuous nanoporous structure with a pore size of ∼67.21–159.33 nm was observed. A series of studies have shown that the addition of sodium dodecyl sulfate (SDS) results in minimum pore size. The coarsen exponent (n) is 1.61, and the activation energy was calculated to be 27.04 kJ mol−1. The ζ'-AgGa alloy prepared at low temperature can be used as the precursor for the preparation of fine np-Ags, and this method provides a new strategy for the industrial production of dealloyed np-Ag.
研究领域:
材料科学
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化学工程
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DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2025
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IF 9
Yuanchuan Liao,Nengwen Gao,Qihong Zhao,Xiaoli Wang,Shumin Lin
Thin-film composite (TFC) membranes prepared by interfacial polymerization (IP) have been widely used in wastewater treatment. The substrate pore size plays a critical role on the polyamide (PA) membrane performance. Herein, a variety of PA nanofiltration (NF) membranes on ceramic substrate with different pore size (5 nm (CM-5), 20 nm (CM-20), 50 nm (CM-50), and 200 nm (CM-200)) are successfully prepared via IP. The results depict that substrates with smaller pores have less absorption capacity of the aqueous monomer (piperazine). Reducing ceramic substrate pore size from 200 nm to 5 nm, the formed PA layer transforms from cracked “ridge-and-valley" structure to continuous film. Cross-sectional FESEM images further demonstrate that the PA layer thickness decreases from 106 nm to 29 nm. The pore size of the polyamide membrane on CM-20 is 0.17 nm with a water permeance of 27.3 L m −2 h −1 bar −1 and Na 2 SO 4 rejection of ∼96 %. A smaller pore size of the ceramic substrate might reduce the available space for monomer movement, increasing the collision and reaction between the monomers, which leads to an elevated cross-linking degree of PA (a denser PA layer). Based on the nanofiltration performance, a ceramic substrate with pore size of 20 nm is optimal for the TFC membrane preparation. The obtained membrane keeps stable performance during 72 h operation. The obtained membrane also demonstrates excellent stability in high-temperature environment and in organic solvent. Overall, ceramic substrate with pore size of 20 nm is selected for the preparation of PA NF membrane via IP. The obtained membrane has broad applications in desalination and environmental remediation.
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Shouwei Zhang,Qiujiao Du,Jinfeng Wang,Yu Huang,Fan Xia
Biological nanopores feature functional elements on the outer surfaces (FEOS) and inner walls (FEIW), enabling precise control over ions and molecules with exceptional sensitivity and specificity. This provides valuable inspiration to scientists for the development of intelligent artificial nanochannel-based platforms, with a wide range of potential applications, including biosensors. Much effort has been dedicated to investigating the distinct contribution of FEOS and FEIW of multichannel membrane biosensors. However, the intricate interactions among neighboring pores in multichannel biosensors have presented challenges. This underscores the untapped potential of single nanochannels as ideal candidates in this field. Here, we employed single nanochannel membranes with different pore sizes to investigate the distinct contributions of FEIW and FEOS to single-nanochannel biosensors, combined with numerical simulations. Our findings revealed that alterations in the negative charges of FEIW and FEOS, induced by target binding, have differential effects on ion transport, contingent upon the degree of nanoconfinement. In the case of smaller pores, such as 20 nm, the ion concentration polarization driven by FEIW can independently control ion transport through the surface’s electric double layer. However, as the pore size increases to 40–60 nm, both FEIW and FEOS become essential for effective ion concentration polarization. When the pore size reaches 100 nm, both FEIW and FEOS are ineffective and thus unsuitable for biosensors. Simulations demonstrate that the observed phenomena can be attributed to the interactions between the charges of FEIW and FEOS within the overlapping electric double layer under confinement. These results underscore the critical role of pore size as a key parameter in governing the functionality of probes within or on nanopore-based biosensors as well as in the design of nanopore-based devices.
研究领域:
生物物理学
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纳米技术
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传感技术
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产品关联
DOI
PubMed
高影响因子
CHEMPHYSCHEM
·
2017
·Vol. 19
·期 1
·130-137
·
IF 3.075
Ting Shi,Yaping Zheng,Tianyu Wang,Peipei Li,Yudeng Wang,Dongdong Yao
Graphical Liquid sponges : Porous liquids are an expanding class of material that has huge potential in gas separation and gas adsorption. Herein, hollow silica nanospheres with different pore sizes were chosen to investigate the influence of porosity size on CO 2 adsorption capacity of porous liquids. Porous liquids are an expanding class of material that has huge potential in gas separation and gas adsorption. Pore size has a dramatic influence on the gas adsorption of porous liquids. In this article, we chose hollow silica nanoparticles as cores, 3-(trihydroxysilyl)-1-propanesulfonic acid (SIT) as corona, and inexpensive industrial reagent polyether amine (M2070) as canopy to obtain a new type of porous liquids. Hollow silica nanospheres with different pore sizes were chosen to investigate the influence of porosity size on CO 2 adsorption capacity of porous liquids. Their chemical structure, morphology, thermal behavior and possible adsorption mechanism are discussed in detail. It was proved that with similar grafting density, porous liquid that has bigger pore size possesses a better CO 2 adsorption capacity (2.182 mmol g −1 under 2.5 MPa at 298 K). More than that, this article demonstrates a more facile and low-cost method to obtain porous liquids with good CO 2 adsorption capacity, recyclability, and huge variability.
研究领域:
材料科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Nano Research
·
2023
·
IF 9.9
Fang Diyi,Liu Chuanlei,Chen Yu,Peng Qilong,Wu Kongguo,Chen Yuxiang,Jiang Hao,Wu Yuan,Shen Benxian,Wu Qiumin,Wu Di,Sun Hui
Porous heterogeneous lyophobic systems (HLSs) find potential applications in energy restoring, dissipating, and absorbing. However, the development of controllable HLSs still lacks rational structure design of nanoporous materials matching the molecular sizes of adopted liquids. Besides that, thoroughly understanding the underlying transportation mechanism in the confined nano channels is greatly challenging. In this work, a series of Co/Zn bimetallic zeolitic imidazolate frameworks (ZIFs) with tunable structures were synthesized via regulating the Co to Zn ratios and employed to investigate the intrusion–extrusion of liquid water in confined nanopores. Structural characterizations confirm the heterometallic coordination in the Co/Zn-doped frameworks. Water intrusion–extrusion experiments unlock the relationship between the intrusion pressure and the nanopore size and realize the evolution of the HLSs between molecular spring and shock-absorber. In addition, cycling tests indicate the reversible structure change of Co/Zn ZIFs encountering pressure-induced water intrusion. In combination with molecular dynamics simulations, we present that the water multimers intrude into nanopores of ZIFs in chain-like forms along with dissociation of hydrogen bonds (HBs). Water molecules in the pre-intrusion state exhibit reduced HBs in response to the increase of pressure and linear structure with 1.6–3.0 HBs on average. After transition to the post-intrusion situation, the associative configuration of water tends to exhibit the tetrahedral structure. Herein, we highlight the roles of pore size and HB in synergically dominating the pressure-induced intrusion–extrusion of liquid water in hydrophobic nanopores. Furthermore, the present work can also guide the development of functional guest–host systems based on porous architectures.
研究领域:
材料科学
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化学工程
·
纳米技术
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产品关联
DOI
高影响因子
Advanced Fiber Materials
·
2022
·Vol. 4
·期 6
·1645-1655
·
IF 12.958
Wang Chunyan,Hou Yaqi,Wang Xingyi,Li Weijun,Zhang Yunmao,Wang Shuli,Zheng Jing,Hou Xu
Catheterization is indispensable in the field of modern medicine. However, catheter-related thrombosis and infections almost inevitably occur during the process, and as drugs can only be administered at the end of catheter, auxiliary strategies are required for successful implantation. Considering these intractable limitations, a type of self-adaptive, anti-coagulate liquid-based fibrous catheter has been developed. More importantly, it has positional drug release property that traditional catheters desperately need but couldn’t attain. Although enlightening, the feasibility and performance of the positional drug release have only been demonstrated by fluorescents, the specific drug release kinetics remains unknown for adaptation to application scenarios. Therefore, we systematically investigate the structural and interfacial effects of drug molecules and fibrous matrixes on drug release kinetics in a liquid-based fibrous catheter. Theoretical calculations and experiments demonstrate that oleophilic and hydrophilic molecules release slowly due to a dissolution-diffusion mechanism. Amphipathic molecules, however, will significantly affect the gating performance by affecting the interfacial stability, hence they release quickly with emulsifying the gating liquid. Besides the significant impact of molecular properties and interfacial effects, matrix pore size also has a slight influence that molecules release faster in bigger pores. Through this study, the liquid-based fibrous catheter may step further toward practical applications including chemotherapy, haemodialysis, angiography, etc . to overcome the existing catheter-related limitations. Graphical Abstract
研究领域:
生物医学工程
·
药剂学
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DOI
高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2023
·Vol. 25
·期 11
·8050-8063
·
IF 3.3
Luyi Wang,Jianjun Zhao,Jun Chen,Tingting Jiang,Qian Zhang,Shengwen Zhong,Sydorov Dmytro
In this study, phenediamine bridging phthalocyanine-based covalent organic framework materials (CoTAPc-PDA, CoTAPc-BDA and CoTAPc-TDA) with increasingly-widening pore sizes are prepared by reacting cobalt octacarboxylate phthalocyanine with p-phenylenediamine (PDA), benzidine (BDA) and 4,4′′-diamino-p-terphenyl (TDA), respectively. The effects of frame size on the morphology structure and its electrochemical properties were explored. X-ray diffraction (XRD), Brunauer–Emmett–Teller (BET) and transmission electron microscopy (TEM) images show that the pore sizes of the CoTAPc-PDA, CoTAPc-BDA and CoTAPc-TDA are about 1.7 nm, 2.0 nm and 2.3 nm, respectively, which are close to the simulated results after geometric conformation optimization using Material Studio software. In addition, the specific surface areas of CoTAPc-PDA, CoTAPc-BDA and CoTAPc-TDA are 62, 81 and 137 m2 g−1, respectively. With increase in the frame size, the specific surface area of the corresponding material increases, which is bound to produce different electrochemical behaviors. Consequently, the initial capacities of the CoTAPc-PDA, CoTAPc-BDA and CoTAPc-TDA electrodes in lithium-ion batteries (LIBs) are 204, 251 and 382 mA h g−1, respectively. As the charge and discharge processes continue, the active points in the electrode material are continuously activated, leading to a continuous increase in charge and discharge capacities. After 300 cycles, the CoTAPc-PDA, CoTAPc-BDA and CoTAPc-TDA electrodes exhibit capacities of 519, 680 and 826 mA h g−1, respectively, and after 600 cycles, the capacities are maintained at 602, 701 and 865 mA h g−1, respectively, with a stable capacity retention rate at a current density of 100 mA g−1. The results show that the large-size frame structure materials have a larger specific surface area and more favorable lithium ion transmission channels, which produce greater active point utilization and smaller charge transmission impedance, thus showing larger charge and discharge capacity and superior rate capability. This study fully confirms that frame size is a key factor affecting the properties of organic frame electrodes, providing design ideas for the development of high-performance organic frame electrode materials.
研究领域:
材料科学
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电化学
·
能源存储
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2013
·
IF 4.612
Hui-Li Lu,Dong-Qiang Lin,Dong Gao,Shan-Jing Yao
Hydrophobic charge-induction chromatography (HCIC) is a novel technology for antibody purification. The ligand densities and pore properties of HCIC resins have significant effects on the separation behavior of protein, however, the understandings are quite limited. In the present work, new HCIC ligand, 2-mercapto-1-methylimidazole (MMI) was coupled to three agarose matrices with different pore sizes. A series of MMI resins with different ligand density and pore size was prepared by the control of ligand coupling. The adsorption isotherms and kinetics on the series of MMI resins were investigated with bovine serum immunoglobulin as the model IgG, and the effects of salt addition were studied. The Langmuir equation and pore diffusion model were used to fit the experimental data, and the influences of ligand density, pore size and salt addition on the saturated adsorption capacity, the dissociation constant and the effective diffusivity were discussed. It was found that the adsorption capacities and the effective pore diffusion coefficient increased with the increase of ligand density and pore size. The effects of salt addition on the adsorption behaviors were dependent on the ligand density. For low ligand density the IgG adsorption was salt-promoted, while the resins with high ligand density showed a salt-independent property. The results indicated that for a given protein the ligand density and pore size of HCIC resins should be optimized for improving the protein adsorption.
研究领域:
生物化学
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蛋白质纯化
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分离科学
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DOI
PubMed
高影响因子
CHINESE JOURNAL OF CATALYSIS
·
2017
·
IF 2.813
Wei Hong,Xiaoqing Yan,Renhong Li,Jie Fan
Stabilizing gold nanoparticles (AuNPs) within a desired size range is critical to realize their promising catalytic performance in many important reactions. Herein, we investigate the anti-sintering properties of cubic mesoporous silica (FDU-12) as a function of pore entrance size. Simple adjustments to the type of organic template and reaction temperature enable the successful synthesis of FDU-12 with controllable entrance sizes (< 3, 3–5 and 7 nm). Excellent anti-sintering properties are observed for FDU-12 with a sub-5-nm entrance size (3–5 nm) over a wide loading concentration (1.0–8.3 wt%) and the AuNPs can be stabilized within a 4.5–5.0-nm range after calcination at 550 °C in air for 5 h. Smaller entrance size (< 3 nm) prevents ingress of 3-nm AuNPs to the mesopores and results in low loading capacity and sintering. Conversely, FDU-12 possessing a larger entrance size (7 nm) shows promising anti-sintering properties at high loading concentrations, although catalytic performance is significantly lost at lower concentrations (e.g. 2.1 wt%, 14.2 ± 5.5 nm). Different anti-sintering mechanisms are proposed for each of the different FDU-12 entrance sizes. Additionally, catalytic data indicates that the obtained 4.5-nm AuNPs supported on FDU-12 with a sub-5-nm entrance size exhibit excellent mass-specific activity (1544 mmol g Au –1 h –1 ) and selectivity (> 99%) at 230 °C for the gas-phase selective oxidation of cyclohexanol.
研究领域:
材料科学
·
催化化学
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DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Chuang Liu,Ruoyu Sun,Jing Zhao,Yixian Hu,Jiliang Mo
The scarcity of freshwater resources has had a significant impact on human life and wildlife survival. Fog harvesting has emerged as one of the most effective solutions to alleviate water scarcity in arid areas. At present, researchers have focused on optimizing hybrid surface designs to capture fog from the air and convert it into water droplets. Here, we prepared a hybrid surface for fog harvesting by a combination of chemical etching and surface modification with mechanical drilling. By adjusting the hole size and the ratio of hydrophilic-superhydrophobic area of the hybrid surfaces, the water collection efficiency was improved. The results indicated that the hole size affected the critical size of sliding water droplets, and the ratio of hydrophilic-superhydrophobic area affected the water droplet sliding speed and water collection frequency, thereby affecting the water collection efficiency per unit time. Therefore, by matching a specific hole size and ratio of hydrophilic-superhydrophobic area, the water collection volume and mobility of water droplets on the hybrid surfaces reached a dynamic equilibrium, achieving the optimal water collection efficiency. When the hole size was 1.0 mm, and the ratio of hydrophilic-superhydrophobic area was 1:4, the highest water collection rate was 805 mg/cm 2 /h, and the water collection efficiency was 17.5 %. By optimizing the hole size and the ratio of hydrophilic-superhydrophobic area, the water collection efficiency of the hybrid surface was maximized. This lays a solid foundation for promoting the practical applications of hybrid surfaces for fog harvesting.
研究领域:
材料科学
·
环境科学
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产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Shi-qi Ao,Shuang-Ping Liu,Jiang Yun,Xiu-Ping Yan,Hai-Long Qian
Tuning the pore size of covalent organic frameworks (COFs) to achieve maximum adsorption performance is an effective way for promoting the enrichment of trace fluoroquinolones (FQs) in food, but it remains challenging. Herein, we prepare three pore-sized sulfonic acid functionalized COFs (SF-COFs), named TPS, TBS, and TSS, to investigate the effect of pore size on the adsorption efficiency of FQs. These three SF-COFs exhibit excellent adsorption capacities for FQs including enrofloxacin (ENR), norfloxacin (NOR), and ciprofloxacin (CIP). Notably, TBS achieves a maximum adsorption capacity of 627.3 mg g -1 for NOR, resulting from its suitable pore size (1.6 times larger than the size of FQs). Accordingly, we further develop a novel TBS based solid-phase extraction to couple with high-performance liquid chromatography for determination of FQs in various foods, which achieves rapid extraction (10 min), low detection limits (0.007-0.01 ng mL -1 ), wide linear range (0.1-1000 ng mL -1 ), and good recoveries (83.7%-105.2%).
研究领域:
分析化学
·
食品安全
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）