JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
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IF 4.6
Zuyao Fu,Ze Chen,Lingfeng Yang,Hao Wang,Jing Xie,Zhaoyang Ding
The misuse of malachite green (MG) has led to significant water pollution, and its metabolite, leucomalachite green (LMG), poses a serious health risk. Rapid detection of drug residues in aquaculture is crucial for food safety. In this study, the dual-emission fluorescent sensor RhB@Tb-MOF (RhB = Rhodamine B) was synthesized based on fluorescence resonance energy transfer (FRET). MG significantly quenches the fluorescence of RhB and affects the fluorescence of Tb-MOF to different extents, making it a suitable ratiometric fluorescent sensor for sensitive MG detection with a limit of detection (LOD) of 9.6 × 10 −3 μg/mL. Although LMG did not affect the fluorescence of RhB@Tb-MOF, it formed a ratio with the fluorescence of RhB@Tb-MOF due to its intrinsic fluorescence properties, and the LOD was 3.5 × 10 −3 μg/mL. Additionally, increasing MG concentrations shifted the sensing system's color from pink to blue, with a colorimetric LOD of 0.15 μg/mL. This paper introduced a colorimetric/fluorescent dual-mode sensor for MG and LMG detection. Furthermore, this sensor was combined with smart labeling technology to achieve rapid on-site quantification of MG, offering significant potential for broad application.
研究领域:
食品安全
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分析化学
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环境科学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Ze Chen,Zuyao Fu,Xiaoyu Du,Jing Xie,Zhaoyang Ding
Simple fluorescence aptasensors were established for malachite green (MG) and leucomalachite green (LMG) detection using G-quadruplex dyes thioflavin T (ThT) and thiazole orange (TO), respectively. ThT and TO, initially non-fluorescent in aqueous solution, exhibited fluorescence upon binding to target-specific aptamers, resulting in the stabilization of G-quadruplex structures. The specific binding between G-quadruplex and MG could induce conformational changes, which caused a pronounced quenching of fluorescence emitted by the intercalator, providing a possibility for detection. The developed assays demonstrated a good linear relationship in the range of 0–1000 μg L -1 for MG and 0–250 μg L -1 for LMG, with low limits of detection of 5.4 × 10 -3 μg L -1 and 0.1 μg L -1 respectively. More importantly, satisfactory detection results in water and fish samples were obtained. Noteworthy was that the uncomplicated fluorescent aptasensors operated effectively without the need for any modifications. The experimental process was facile and realized in homogeneous solution, which exhibited quick responsiveness after simple mixing.
研究领域:
生物化学
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分析化学
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食品安全
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产品关联
DOI
高影响因子
Journal of Analysis and Testing
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2020
·Vol. 4
·期 4
·264-272
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IF 0
Jiang Li-Ping,Li Na,Liu Lin-Qi,Zheng Xian,Du Fu-You,Ruan Gui-Hua
Abstract Polymerized high internal phase emulsion (polyHIPE) monoliths were prepared and applied as adsorbent materials for solid-phase extraction (SPE) of malachite green (MG) and leucomalachite green (LMG) from water samples. The polyHIPE monoliths were prepared by post-functionalization of monolithic surface with 6-aminocaproic acid (ACA) via ring opening reaction of epoxy groups in glycidyl methacrylate (GMA)-based polyHIPEs, and then applied to the preconcentration and determination of trace MG and LMG in environmental water samples by combing with high-performance liquid chromatography (HPLC). Taking MG and LMG as targets, main factors affecting SPE performance of the polyHIPE monoliths were investigated. Under the optimized conditions, the ACA-functionalized polyHIPE monoliths could effectively preconcentrate MG and LMG from 150 mL of water samples, and the recoveries of MG and LMG at three spiked levels were ranged from 84.8 to 97.4% with the relative standard deviations (RSDs) lower than 6%. The proposed method exhibited good linearity in the range of 2–200 ng mL –1 , with low limits of detection of 17.0 and 8.7 pg mL –1 for MG and LMG, respectively. In addition, the prepared ACA-modified polyHIPE monolith showed good durability and stability, and it could be reused for 200 cycles without obvious losing the extraction performance. Graphic abstract
研究领域:
环境科学
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分析化学
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产品关联
DOI
DYES AND PIGMENTS
·
2018
·
IF 3.767
Ji Yang,Hao Wu,Ming-Hui Wu,Jun Zeng,Zheng-Zhong Lin,Xiao-Mei Chen,Zhi-Yong Huang
Malachite green (MG) is an industrial dye, but has been once abused in aquaculture for disease treatment. Because MG and its metabolite, leucomalachite green (LMG), present great harm to human health, it is essential to rapidly and accurately detect MG and LMG residues in aquatic products. However, the simultaneous screening of MG and LMG is still a challenge to date. In this work, a dual-template molecularly imprinted polymer (MIP) coated CdTe QDs was developed and applied as a nano-probe to simultaneously detect MG and LMG in fish samples via normal and synchronous fluorescence quenching. The molar ratios of templates (MG and LMG) to monomer (APTES) and cross-linker (TEOS), and the amount of CdTe QDs in the probe preparation were optimized. A normal fluorescence emission at 600 nm could be linearly quenched by MG, while the synchronous fluorescence emission at the wavelength interval of 340 nm could be quenched by both LMG and MG. The detection limits of MG and LMG were 17 nmol L −1 and 28 nmol L −1 (3 σ , n = 9), respectively. Based on the equations of Stern-Volmer, MG and LMG could be simultaneously detected without any conversion treatment. The recoveries of MG and LMG in fish samples ranged from 92% to 107% and from 91% to 108%, respectively. The results demonstrated that the as-prepared dual-template CdTe@MIP could be used as a fluorescent probe to rapidly and simultaneously detect MG and LMG in fish samples.
研究领域:
分析化学
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食品安全
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
·
IF 9.221
Jing Wu,Xu Jing,Yong Liu,Luyao Zhao,Xuemeng Ji,Hanyue Fu,Bowei Zhang,Yan Zhang,Shuo Wang
Malachite green (MG) and its metabolite leucomalachite green (LMG) are aquaculture-banned drugs whose rapid detections are required yet hindered by the use of expensive instruments as well as the tedious pretreatment procedures for aquatic samples. An integrated workflow for total MG (MG and LMG) analysis in aquatic samples is reported combining a deep eutectic solvent (DES) as the extractant and a paper origami-based micro-total electrochemical immunoassay (μTEI). DES solution (mixture of glycine betaine and ethylene glycol) enables the extraction of MG and LMG with high efficiency from tissue samples and allows grease for separation. The μTEI is compartmentalized simply by manual-folding to implement multiple analytical steps in a programmable manner, including impurity removal, LMG oxidation , MG enrichment, and electrochemical analysis . The entire workflow can be accomplished in 1.0 h, showing a wide linear range of 0.5–100 ppb, a low limit of detection of 0.07 ppb, and a high recovery rate of 78.9–97.6%. The μTEI offers a solution for the rapid accurate monitoring of total MG in aquatic samples and is potentially available for the analysis of other contaminants with proper modifications.
研究领域:
分析化学
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环境科学
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生物技术
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食品科学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD AND DRUG ANALYSIS
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2022
·
IF 6.157
Jia Wang,Chengyi Hong,Zhengzhong Lin,Zhi-Yong Huang
A simple fluorescence method to quantitatively monitor malachite green (MG) was introduced based on the conformation switch effect of MG-aptamer (Apt) and the fluorochrome of SYBR Green I (SGI). SGI can embed in the stem-loop structure of Apt to produce fluorescence. However, the fluorescence will be quenched when MG is introduced because of the specific binding of Apt to MG. Under the optimal conditions, the fluorescence method displayed excellent linear relationship between the fluorescence quenching efficiencies and the MG concentrations ranging from 0.02 μmol L −1 to 1.0 μmol L −1 . The detection limit of the method was 0.67 μg kg −1 (3 α / κ , n = 9) which is lower than the limit (1 μg kg −1 ) specified in the national standard of China (GB/T 19857–2005) and the limit (2 μg kg −1 ) specified by the European Food Safety Authority (EFSA, 2016). Excellent recoveries between 87.1% and 106.9% tested by spiking standards were achieved with RSD less than 6.4%. The data were confirmed by the method of HPLC, and the results proved that the established fluorescence method could be used for the rapid detection of MG in fish with high accuracy and sensitivity.
研究领域:
分析化学
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食品安全
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生物传感
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产品关联
DOI
高影响因子
MATERIALS CHEMISTRY AND PHYSICS
·
2019
·
IF 2.781
Jianli Tao,Meng Zhang,Xiang Gao,Hao Zhao,Zhenpeng Ren,Donghui Li,Jiaojiao Li,Ruiqin Zhang,Yonggang Liu,Yunpu Zhai
Co 3 O 4 /red phosphorus (Co 3 O 4 /RP) photocatalysts were synthesized via facile hydrothermal and mechanically grinding approach. The catalysts were characterized via XRD , TEM , XPS , N 2 sorption , photoelectro chemical measurements and UV–vis DRS . The results showed that the Co 3 O 4 nanocrystals were assembled on the surface of the RP. The loading of Co 3 O 4 on RP was beneficial to enhance the light absorption compared with pure RP. Under visible light , 94.5% of malachite green (MG) can be photodegraded within 20 min over 10% Co 3 O 4 /RP composite, while only 17.3% and 59.9% of MG can be photodegraded over pure Co 3 O 4 and RP. The reaction rate constant k obtained over 10% Co 3 O 4 /RP heterojunction (0.1464 min −1 ) is approximately 3.2 times that of pure RP (0.0457 min −1 ), and 15.3 times that of bare Co 3 O 4 (0.0096 min −1 ). The cycle experiments showed that the catalyst had high photocatalytic stability. Co 3 O 4 nanocrystals inhibit the photoelectron-hole pair recombination of red phosphorus semiconductor, and exhibit improved visible light absorption . Consequently, the catalytic performance of Co 3 O 4 /RP has been enhanced under visible light .
研究领域:
材料科学
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环境科学
✓ 已验证
DOI
高影响因子
Desalination and Water Treatment
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2024
·
IF 1
Lincai Peng,Jing Gao,Shun Yao,Xianqiu Lan,Huaiping Li,Hang Song
ABSTRACT As a common agriculture waste as well as available biomass resource from ginkgo tree, ginkgo leaves were used as adsorbent to remove two models of cationic dyes methyl violet (MV) and malachite green (MG) after adequate modification. As the result, hydroxylated magnetic ginkgo leaf powder and carboxylated magnetic ginkgo leaf powder were obtained and characterized, respectively, which had the potential to be an environmentally friendly and economically effective biosorbents for the adsorption of dyes. These new biosorbents exhibited high adsorption capacity for dyes, with the maximum adsorption capacity of 450 mg g –1 for MV and 231 mg g –1 for MG. Further investigation confirmed that these adsorption processes were fitted with pseudo-second-order and Langmuir isothermal model. Moreover, these adsorbents with remarkable magnetism could be easily and rapidly recovered by external magnet after their use. The study demonstrated that the promising and reliable modified biosorbents were very suitable for removal of cationic dyes with high concentration at lower cost.
研究领域:
环境科学
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生物技术
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2021
·
IF 4.556
Qin Hu,Yikun Cui,Longfei Zhang,Minjie Qian,Lixia Xiao,Ming Yang,Zhen-Quan Yang,Shengqi Rao,Xiaojuan Gong,Jie Han
In this work, a novel fluorescence method based on an orange-emissive carbon dots (O-CNDs) was for the first time reported for the rapid and ultrasensitive determination of malachite green (MG). The O-CNDs were simply produced via a one-pot hydrothermal treatment of rhodamine B. The detection mechanism was based on the interaction between O-CNDs and MG, where the fluorescence of O-CNDs was suppressed by MG via a combined effect of inner filter effect (IFE) and dynamic suppressing. Under the optimum sensing conditions, the O-CNDs were found to display rapid reaction time (1.0 min), high selectivity and excellent sensitivity with two linear concentration ranges of 0.01–0.1 and 0.1–10.0 μmol L −1 , and the corresponding detection limits were as low as 0.08 and 0.93 nmol L −1 , respectively. The developed fluorescence method was successfully applied for the determination of MG in fish samples, including silver carp, blackfish, blunt-snout bream, common carp and grass carp, with satisfactory results; the recoveries were in a range of 96.0–104.8 % and the relative standard deviations (RSDs) below 4.54 %. The results of this study validated the great potential of O-CNDs as an effective fluorescence sensing agent for MG monitoring in aquatic samples.
研究领域:
分析化学
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环境科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Yaoping Hu,Zhijin Gao,Junfei Luo
Facile detection of malachite green (MG), a toxic dye, in aquaculture is urgently demanded for environment and food safety. Herein, we design a novel fluorescent probe, namely red emissive Se,N,Cl-doped carbon dots (CDs), to accurately determinate MG. CDs are prepared by hydrothermal treatment of selenourea and o-phenylenediamine in HCl solution. This material exhibits excitation-independent dual emissions at 625 and 679 nm, with a high quantum yield of 23.6%. A selective and sensitive fluorescent sensor toward MG is established based on inner filter effect, because both the excitation and emission light of CDs can be strongly absorbed by MG. The fluorescence quenching of CDs is linear to the MG concentration over the range of 0.07–2.50 μM with a low detection limit of 21 nM. Trace-level analysis of MG in fish tissue is successfully explored, demonstrating the great potential of the proposed sensor for MG monitoring in aquatic products.
研究领域:
环境科学
·
食品安全
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2015
·Vol. 182
·期 9
·1791-1796
·
IF 3.741
Li Lu,Lin Zheng-zhong,Chen Xiao-mei,Zhang Hong-yuan,Lin Yi-dong,Lai Zhu-zhi,Huang Zhi-yong
Molecularly imprinted polymer (MIP) particles for malachite green (MG) were prepared by emulsion polymerization using methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the cross-linker, and a combination of Span-80 and Tween-80 as an emulsifier. The MIP particles were characterized by SEM micrographs and FT-IR spectra. Their binding capacity for MG was evaluated based on kinetic and isothermal adsorption experiments and compared to non-imprinted polymer particles. Analytical figures of merit include an adsorption equilibrium time of 15 min, an adsorption capacity of 1.9 mg∙g‾ 1 in acetonitrile-water (20:80), and an imprinting factor of 1.85. The MIP particles were successfully applied to the extraction of MG from fish samples spiked with MG and the other interfering substances prior to its determination of MG by HPLC. Spiked samples gave recoveries of MG that ranged from 86 to 104 %, much higher than that of the other interfering substance.
Molecularly imprinted polymer particles for malachite green (MG) were prepared by emulsion polymerization. They show adsorption capacity of 1.9 mg∙g‾¹ with imprinting factor of 1.85, and were successfully applied to the extraction of MG from spiked fish samples.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Jian Song,Shuyu Lai,Dingnan Wang,Xiaomei Wu,Yiming Zhang,Linjiang Pang,Tingting Chai,Ji Zhao,Xiaodong Wang
The development of simultaneous and rapid screening of the residues of veterinary drugs and their metabolites is up to now still a challenge. In this study, a novel strategy of read-out analysis is proposed based on high-performance thin-layer chromatography (HPTLC) separation coupled with self-visualization detection of trace of leucomalachite Green (LMG) and malachite green (MG) in aquatic products. The novel self-visualization nanomaterial was successfully synthesized and subsequently fully characterized. The ionic associate MG–I 3 formed on the visualization sensing interface was effectively attested by MALDI-TOF/TOF-MS for the first time. A serials of visualization reactions were conducted on the target zone surface of HPTLC after separation. Under the optimum conditions, the calibration curves of LMG and MG were obtained with good linearity in the range of 0.25–8.0 μg mL −1 and 0.125–4.0 μg mL −1 , respectively. The limits of detection and limit of quantification were calculated as 1.71 and 5.19 μg kg −1 for LMG and 0.9 and 2.74 μg kg -1 for MG, respectively. Real sample analysis and validation provided satisfactory results. The proposed method has the potential to detect simultaneously, rapidly, and accurately LMG and MG in aquatic products during storage, handling, and sales.
研究领域:
分析化学
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食品科学
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兽医学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）