JOURNAL OF PEST SCIENCE
·
2014
·Vol. 88
·期 1
·25-35
·
IF 2.664
Toepfer Stefan,Zellner Michael,Szalai Mark,Kuhlmann Ulrich
The western corn rootworm ( Diabrotica virgifera virgifera ; Coleoptera: Chrysomelidae) is a major maize pest in North America and Europe. For developing population models to better understand this key pest, survival rates need to be known. In contrast to the reported field survival of eggs, the three larval instars, and pupae, the survival of the adults had not been assessed under field conditions. Weekly survival of adults was studied in large walk-in gauze cages in two fields in Hungary between 2009 and 2011. Each cage contained a combination of maize with two out of nine other crops. The number of adult beetles rapidly decreased with time following an exponential-like survivorship curve, with a weekly mortality varying between 31 and 61 %. This is in contrast to laboratory experiments, where populations decreased with time following a linear-like curve. The average lifespan was only 19 days with a maximum of 84 days. After 14 days, 55 % of adults had died, thus, having had no chance to lay eggs because of the pre-oviposition period required. Maize appeared to be the most appropriate crop for survival. Combining maize with other crops that could serve as alternative food did not enhance survivorship, and many combinations were in fact disadvantageous. Crop and weed coverage played a minor role in influencing survival, and only few weeds appeared slightly advantageous. It is argued that the role of alternative food sources for D. v. virgifera adults might be overestimated. Survivorship equations are suggested to support modelling of population dynamics of this pest and to support timing of direct control measures against the adults to prevent their egg laying and therefore damage to maize roots the following year.
研究领域:
昆虫学
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农业害虫管理
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种群动态模型
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Pingli Dai,Zhenxiong Yan,Shilong Ma,Yang Yang,Qiang Wang,Chunsheng Hou,Yanyan Wu,Yongjun Liu,Qingyun Diao
Effects of glyphosate on survival, developmental rate, larval weight, and midgut bacterial diversity of Apis mellifera were tested in the laboratory. Larvae were reared in vitro and fed diet containing glyphosate 0.8, 4, and 20 mg/L. The dependent variables were compared with negative control and positive control (dimethoate 45 mg/L). Brood survival decreased in 4 or 20 mg/L glyphosate treatments but not in 0.8 mg/L, and larval weight decreased in 0.8 or 4 mg/L glyphosate treatments. Exposure to three concentrations did not affect the developmental rate. Furthermore, the intestinal bacterial communities were determined using high-throughput sequencing targeting the V3–V4 regions of the 16S rDNA. All core honey bee intestinal bacterial phyla such as Proteobacteria (30.86%), Firmicutes (13.82%), and Actinobacteria (11.88%) were detected, and significant changes were found in the species diversity and richness in 20 mg/L glyphosate group. Our results suggest that high concentrations of glyphosate are deleterious to immature bees.
研究领域:
生态毒理学
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微生物学
·
昆虫学
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产品关联
DOI
PubMed
高影响因子
AQUACULTURE
·
2025
·
IF 3.9
Fei Yang,Zhiqiang Zhang,Shenye Qu,Gaoxue Wang,Qing Yu,Fei Ling
Micropterus salmoides rhabdovirus (MSRV) is a prominent pathogen that mainly endangers largemouth bass fry, which often causes devastating diseases to largemouth bass and leads to huge economic losses in aquaculture. So far, there is no approved effective measure to control MSRV infection. In the study, 24 magnolol derivatives were used to conduct anti-MSRV activity screening by RT-qPCR, and the results indicated that 5,5′-diallyl-3,3′-dinitro-[1,1′-biphenyl]-2,2′-diol (M3) exhibited the best anti-MSRV efficiency with IC 50 of 0.92 μM. Moreover, further study showed that the CPE induced by MSRV was obviously weaken by M3, and the viral titers were also significantly decreased by M3 within 96 h after MSRV infection ( p < 0.05). Next, the anti-MSRV mechanism of M3 was preliminarily investigated, and the findings illustrated that M3 did not directly act on MSRV virion which had no effect on the infectivity of MSRV. Furthermore, the adsorption of MSRV onto GCO cells was not hindered by M3. The results of cell cycle detected by flow cytometry demonstrated that M3 altered the state of cell cycle arrest in S phase induced by MSRV, influencing the environment required for virus replication. In addition, observation of survival rate and determination of viral load were conducted to further verify the antiviral activity of M3 against MSRV. The results illustrated that administration with M3 at the dose of 20 mg/kg via intraperitoneal injection significantly enhanced the survival rate of largemouth bass infected with MSRV by 35.98 % ( p < 0.01). Moreover, the viral loads of liver, spleen and kidney were significantly decreased by M3 on day 3 and day 5 post MSRV infection ( p < 0.05). Finally, the antiviral effects of M3 against aquatic rhabdoviruses spring viremia of carp virus (SVCV) and infectious hematopoietic necrosis virus (IHNV) were explored, and the results demonstrated that M3 evidently lessened the production of CPE induced by SVCV and IHNV, and inhibited the proliferation of SVCV and IHNV in EPC cells in a dose-dependent manner, with IC 50 of 2.92 μM and 2.78 μM, respectively. To sum up, M3 exhibits a high antiviral activity against MSRV, and is expected to be developed into antiviral specific drugs against rhabdoviruses in aquaculture.
研究领域:
水产养殖学
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病毒学
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药理学
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产品关联
DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 21
·3468
·
IF 5.561
Shumao Cui,Ziyi Pan,Sheng Wu,Bingyong Mao,Xin Tang,Qiuxiang Zhang,Hao Zhang,Jianxin Zhao
The influence of surface substance production on the freeze-drying survival ofLactobacillus caseiand methods to control the surface substances during fermentation were studied. The bacteria were treated with hypertonicity combined with ultrasound, and the survival rate was determined. The optimal conditions for removing surface substance without harming the bacteria were 81 w/18 min. The surface substances provided a protective effect on the lyophilization of the bacteria without protectants. However, in the presence of protectants, excessive surface substances reduced the protective effect of the optimum protectant alginate to 39.69 ± 1.27%. Finally, the amount of surface substances and lyophilized survival rate of collected bacteria were determined by adding EDTA during fermentation and regulating fermentation conditions, such as the carbon source, carbon-to-nitrogen ratio, and pH. The highest survival rate was 85.79 ± 3.29%, which was achieved when the amount of surface substances was (2.82 ± 0.55) × 10−11mg/CFU. Therefore, the production of surface substances by the bacteria could be reduced by modifying the fermentation stage, which has significance in the improvement of the lyophilization survival rate ofL. caseiand the number of live bacteria per unit mass ofL. caseiin the lyophilized preparation.Keywords:lyophilization survival rate;surface substance;Lactobacillus casei;fermentation conditions
研究领域:
微生物学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Cell and Developmental Biology
·
2025
·
IF 4.3
Weijie Li,Xi Yang,Wenyan Bi,Liyong Song,Baolin Liu
Developing bioartificial liver and hepatocyte transplantation technology causes increasing hepatocyte cell demand. Effective long-term hepatocyte cell preservation methods are necessary to promote. Progressive cooling is a key preservation technology for cell banks. However, the cell solution needs to be supercooled in a slow freezing process. The high degree of supercooling possibly induces uncontrollable intracellular ice formation. This work designs an ultrasonic ice-seeding system for L-02 hepatocyte cell preservation, reducing supercooling and improving cell survival rate. The effect of ultrasonic intensities on the hepatocyte’s survival rate was investigated and optimized. The results prove the calorimetric method can efficiently measure the ultrasonic intensity dissipated in the hepatocyte cell preservation solution. When the ultrasonic intensity is 0.0329 W/cm 2 ∼ 0.4316 W/cm 2 , the hepatocyte survival rate is over 90%. There is no significant difference between experiment groups (p < 0.05) when the ultrasonic intensity is larger than 0.4316 W/cm 2 . The hepatocyte cell survival rate reduced significantly with the increase of ultrasonic intensity. The 7-day hepatic function indicator experiment results indicate that the ultrasonic ice seeding has the weakest impact on hepatocyte cells in the four groups. The secretion of urea, albumin and glucose proved that ultrasonic ice seeding technology does not affect cell secretion and has an enormous advantage in cryopreservation. It can be widely applied to cell freezing fields.
研究领域:
生物技术
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医学工程
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Kun Feng,Ru-meng Huang,Rui-qing Wu,Yun-shan Wei,Min-hua Zong,Robert J. Linhardt,Hong Wu
To improve the survivability of probiotics under the harsh conditions, a novel double-layered vehicle, which was developed by a one-step coaxial electrospinning procedure, was here used to encapsulate the probiotics. The morphology characterization analysis revealed that the electrospun fiber had a beaded morphology and core-shell structure. Probiotic cells were successfully encapsulated in the fibers (10 7 CFU/mg) and exhibited an oriented distribution along the fiber. Additionally, the encapsulation of core-shell fiber mat enhanced the tolerance of probiotic cells to simulated gastrointestinal conditions and no significant loss of viability was found (p > 0.05). Besides that, the encapsulated cells exhibited better thermal stability under heat moisture treatment, lower loss of viability (0.32 log CFU/mL) was occurred when compared with the free cells or encapsulated cells in uniaxial fiber mat. In conclusion, this double-layered vehicle presents a great potential in probiotic encapsulation and improving their resistant ability to the harsh conditions.
研究领域:
食品科学
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生物工程
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产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 9
·1203
·
IF 5.561
Jiao Shi,Shu-Fang Li,Kun Feng,Shuang-Yan Han,Teng-Gen Hu,Hong Wu
For improving probiotics’ survivability under harsh conditions, this study usedLactiplantibacillus plantarumGIM1.648 as a model microorganism to investigate its ability to produce biofilms on electrospun ethyl cellulose nanofiber mats. SEM observations confirmed that biofilm was successfully formed on the nanofibers, with the latter being an excellent scaffold material. The optimal cultivation conditions for biofilm formation were MRS medium without Tween 80, a culture time of 36 h, a temperature of 30 °C, a pH of 6.5, and an inoculum concentration of 1% (v/v). The sessile cells in the biofilm exhibited improved gastrointestinal and thermal tolerance compared to the planktonic cells. Additionally, the RT-qPCR assay indicated that theluxSgene played a crucial role in biofilm formation, with its relative expression level being 8.7-fold higher compared to the planktonic cells. In conclusion, biofilm formation on electrospun nanofiber mat has great potential for improving the viability of probiotic cells under harsh conditions.Keywords:electrospun nanofiber mat;biofilm;probiotics;viability;luxSgene
研究领域:
微生物学
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食品科学
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材料科学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2017
·Vol. 82
·期 8
·1978-1984
·
IF 1.815
Huan bin Zhou,Jiashu Chen,Shunyi Li,Jianpan Zhang,Chun e Zhu,Hao Ran,Meihua Luo,Xin Pan,Haiyan Hu,Chuanbin Wu
en This link goes to a English section pt This link goes to a English section Microencapsulation is an effective technology used to protect probiotics against harsh conditions. Extrusion is a commonly used microencapsulation method utilized to prepare probiotics microcapsules that is regarded as economical and simple to operate. This research aims to prepare acid-resistant probiotic microcapsules with high viability after freeze-drying and optimized storage stability. Streptococcus thermophilus IFFI 6038 (IFFI 6038) cells were mixed with trehalose and alginate to fabricate microcapsules using extrusion. These capsules were subsequently coated with chitosan to obtain chitosan-trehalose-alginate microcapsules with shell-matrix structure. Chitosan-alginate microcapsules (without trehalose) were also prepared using the same method. The characteristics of the microcapsules were observed by measuring the freeze-dried viability, acid resistance, and long-term storage stability of the cells. The viable count of IFFI 6038 in the chitosan-trehalose-alginate microcapsules was 8.34 ± 0.30 log CFU g −1 after freeze-drying (lyophilization), which was nearly 1 log units g −1 greater than the chitosan-alginate microcapsules. The viability of IFFI 6038 in the chitosan-trehalose-alginate microcapsules was 6.45 ± 0.09 log CFU g −1 after 120 min of treatment in simulated gastric juices, while the chitosan-alginate microcapsules only measured 4.82 ± 0.22 log CFU g −1 . The results of the long-term storage stability assay indicated that the viability of IFFI 6038 in chitosan-trehalose-alginate microcapsules was higher than in chitosan-alginate microcapsules after storage at 25 °C. Trehalose played an important role in the stability of IFFI 6038 during storage. The novel shell-matrix chitosan-trehalose-alginate microcapsules showed optimal stability and acid resistance, demonstrating their potential as a delivery vehicle to transport probiotics. Practical Application en This link goes to a English section pt This link goes to a English section The construction of acid-resistant shell-matrix microcapsules is a novel assay in the development and application of microencapsulation technology in probiotics. The method of embedded cryoprotectants in microcapsules enhances the thermal stability and freeze-dried viability of enzymes in Streptococcus thermophiles compared to conventional methods that simply mix the cryoprotectants and microcapsules together.
研究领域:
食品科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Zhikun Yang,Mingrui Li,Yanxiao Li,Xin Wang,Zhihua Li,Jiyong Shi,Xiaowei Huang,Xiaodong Zhai,Xiaobo Zou,Yunyun Gong,Melvin Holmes,Megan Povey,Jianbo Xiao
The gelatin/gellan gum based-bilayer emulsion film was developed in this work to improve the survivability of Bifidobacterium longum during the storage process. The baobab seed oil (BO) was added to the gelatin (GE) matrix to develop emulsion film as the barrier outer layer. The blueberry anthocyanin extract (BE) was incorporated into the gellan gum (GG)-based inner layer to enhance the viability of B. longum . The SEM and FTIR results revealed that the probiotics were successfully entrapped in BO/BE-loaded bilayer film. The greatest survivability and viable cell numbers of the B. longum during the storage period were observed in the BO/BE loaded bilayer film. Furthermore, the stability of the colorful patterns by electrochemical writing was also evaluated in this work. Finally, the GE/BO-GG/BE/BM maintain satisfactory probiotic viability in steamed bread coating application. Hence, the GE/BO-GG/BE/BM bilayer film could be considered a novel material to deliver and protect the probiotics in food applications.
研究领域:
食品科学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
AQUACULTURE
·
2024
·
IF 3.9
Jinjie Zhang,Jinlu Huang,Shanzhen Chen,Jing Tan,Xingyi Zhu,Yanqi Gao,Hui Zeng,Yong-An Zhang,Yang Zhou
Aeromonas schubertii is a global pathogen of fish that causes internal nodular diseases and death in severe cases. In the present study, we isolated nine pathogenic strains from diseased hybrid snakehead ( Channa argus ♂ × Channa maculata ♀) which were formally identified as A. schubertii by molecular identification, including 16S rRNA and gyrB gene sequencing. Subsequently, we determined the extracellular enzyme activity of six isolates and compared pathogenicity to investigate potential relationships with geographic characteristics. Two highly pathogenic strains, AS-14 and AS-16, were finally identified as inactivated vaccine candidates. Subsequently, we evaluated different concentrations of the antigen for each vaccine by performing experiments in hybrid snakehead; fish were inoculated with inactivated vaccine via the intraperitoneal route and a booster injection was administered 14 days later. Relative percent survival (RPS) for fish immunized with high doses of the two vaccines (1 × 10 8 CFU/fish) were 54.4% and 57.4%, respectively; these values were higher than other groups of fish receiving different doses (1 × 10 6 and 1 × 10 7 CFU/fish). The bacterial load of tissues harvested from the fish immunized with high doses of the vaccine groups were significantly lower than those of fish immunized with low doses. Titers of specific antibodies against A. schubertii in fish immunized with a high dose of AS-16 vaccine were maintained at high levels when tested six weeks after the booster injection. Furthermore, immune-related genes in the spleen, including MHC I, MHC II, CD4, CD8α and IgM , all exhibited high levels of expression, thus suggesting that hybrid snakehead underwent an adaptive immune response post-vaccination. Collectively, our findings enrich our understanding of the specific causative agents underlying nodular diseases in hybrid snakehead and provide scientific reference guidelines for the development of vaccines against A. schubertii . The development of such vaccines will be conducive to the sustainable development of hybrid snakehead in commercial aquaculture.
研究领域:
水产养殖学
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微生物学
·
免疫学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Qing Zhang,Yujin Yang,Yannan Chen,Shuoshuo Ban,Shaobin Gu,Fang Li,Mengmeng Xue,Jianrui Sun,Xin Li,Shanshan Tie
The purpose of this study was to prepare a pH-responsive microgel for co-delivering Weizmannia coagulans 99 (BC99) and procyanidins (PCs) to enhance the survival rate and tolerance of probiotics in complex micro-environment. The effects of different concentrations of PCs on the properties of microgels were optimized, and found that the spherical microgels had higher encapsulation efficiency (90.27 ± 2.51%) and smaller size when the concentration of PCs was 20 μg/mL. The interaction among PCs, pectin and protein could effectively improve the survival rate of BC99 under different pH, bile salt, digestive enzyme and temperature conditions, maintain their stability in acidic gastric fluid, and realize the release of probiotics in neutral intestinal fluid. Moreover, the microgel was able to protect BC99 against H 2 O 2 and antibiotics. This work provides a pH-responsive co-loaded microgels for BC99 and PCs, and has the potential in the loading and delivery of other probiotics and polyphenols.
研究领域:
微生物学
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食品科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Mei Liu,Le Sun,Guolan Qin,Jin Zhang,Yangkun Wei,Yunpeng Shen,Hao Ma,Wenqian Dang,Jie Li,Ying Liang,Limin Li,Xueling Zheng,Chong Liu
The fermentation capacity of frozen dough determines its handling properties and the quality of final product. In this study, the effects of sanxan on the fermentation capacity of frozen dough during freeze-thawed cycles (FTCs) were investigated. The results showed that 0.5% sanxan improved the fermentation capacity of frozen dough during FTCs. An increase in yeast survival rate indicated that frozen dough with 0.5% sanxan (sanxan-dough) inhibited the death of yeast and improved the gas-production ability of yeast. After 8FTCs, compared with control-dough, the disulfide bond content of sanxan-dough significantly increased, the stability of secondary structure was enhanced, the continuity and strength of gluten network were improved; these results indicated that 0.5% sanxan enhanced the gas-holding ability of frozen dough. This study explored a new horizon for the fermentation capacity’ improvement of frozen dough by sanxan in term of yeast and gluten proteins.
研究领域:
食品科学
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微生物学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）