JOURNAL OF PEST SCIENCE
·
2014
·Vol. 88
·期 1
·25-35
·
IF 2.664
Toepfer Stefan,Zellner Michael,Szalai Mark,Kuhlmann Ulrich
The western corn rootworm ( Diabrotica virgifera virgifera ; Coleoptera: Chrysomelidae) is a major maize pest in North America and Europe. For developing population models to better understand this key pest, survival rates need to be known. In contrast to the reported field survival of eggs, the three larval instars, and pupae, the survival of the adults had not been assessed under field conditions. Weekly survival of adults was studied in large walk-in gauze cages in two fields in Hungary between 2009 and 2011. Each cage contained a combination of maize with two out of nine other crops. The number of adult beetles rapidly decreased with time following an exponential-like survivorship curve, with a weekly mortality varying between 31 and 61 %. This is in contrast to laboratory experiments, where populations decreased with time following a linear-like curve. The average lifespan was only 19 days with a maximum of 84 days. After 14 days, 55 % of adults had died, thus, having had no chance to lay eggs because of the pre-oviposition period required. Maize appeared to be the most appropriate crop for survival. Combining maize with other crops that could serve as alternative food did not enhance survivorship, and many combinations were in fact disadvantageous. Crop and weed coverage played a minor role in influencing survival, and only few weeds appeared slightly advantageous. It is argued that the role of alternative food sources for D. v. virgifera adults might be overestimated. Survivorship equations are suggested to support modelling of population dynamics of this pest and to support timing of direct control measures against the adults to prevent their egg laying and therefore damage to maize roots the following year.
研究领域:
昆虫学
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农业害虫管理
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种群动态模型
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Pingli Dai,Zhenxiong Yan,Shilong Ma,Yang Yang,Qiang Wang,Chunsheng Hou,Yanyan Wu,Yongjun Liu,Qingyun Diao
Effects of glyphosate on survival, developmental rate, larval weight, and midgut bacterial diversity of Apis mellifera were tested in the laboratory. Larvae were reared in vitro and fed diet containing glyphosate 0.8, 4, and 20 mg/L. The dependent variables were compared with negative control and positive control (dimethoate 45 mg/L). Brood survival decreased in 4 or 20 mg/L glyphosate treatments but not in 0.8 mg/L, and larval weight decreased in 0.8 or 4 mg/L glyphosate treatments. Exposure to three concentrations did not affect the developmental rate. Furthermore, the intestinal bacterial communities were determined using high-throughput sequencing targeting the V3–V4 regions of the 16S rDNA. All core honey bee intestinal bacterial phyla such as Proteobacteria (30.86%), Firmicutes (13.82%), and Actinobacteria (11.88%) were detected, and significant changes were found in the species diversity and richness in 20 mg/L glyphosate group. Our results suggest that high concentrations of glyphosate are deleterious to immature bees.
研究领域:
生态毒理学
·
微生物学
·
昆虫学
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DOI
PubMed
高影响因子
AQUACULTURE
·
2025
·
IF 3.9
Fei Yang,Zhiqiang Zhang,Shenye Qu,Gaoxue Wang,Qing Yu,Fei Ling
Micropterus salmoides rhabdovirus (MSRV) is a prominent pathogen that mainly endangers largemouth bass fry, which often causes devastating diseases to largemouth bass and leads to huge economic losses in aquaculture. So far, there is no approved effective measure to control MSRV infection. In the study, 24 magnolol derivatives were used to conduct anti-MSRV activity screening by RT-qPCR, and the results indicated that 5,5′-diallyl-3,3′-dinitro-[1,1′-biphenyl]-2,2′-diol (M3) exhibited the best anti-MSRV efficiency with IC 50 of 0.92 μM. Moreover, further study showed that the CPE induced by MSRV was obviously weaken by M3, and the viral titers were also significantly decreased by M3 within 96 h after MSRV infection ( p < 0.05). Next, the anti-MSRV mechanism of M3 was preliminarily investigated, and the findings illustrated that M3 did not directly act on MSRV virion which had no effect on the infectivity of MSRV. Furthermore, the adsorption of MSRV onto GCO cells was not hindered by M3. The results of cell cycle detected by flow cytometry demonstrated that M3 altered the state of cell cycle arrest in S phase induced by MSRV, influencing the environment required for virus replication. In addition, observation of survival rate and determination of viral load were conducted to further verify the antiviral activity of M3 against MSRV. The results illustrated that administration with M3 at the dose of 20 mg/kg via intraperitoneal injection significantly enhanced the survival rate of largemouth bass infected with MSRV by 35.98 % ( p < 0.01). Moreover, the viral loads of liver, spleen and kidney were significantly decreased by M3 on day 3 and day 5 post MSRV infection ( p < 0.05). Finally, the antiviral effects of M3 against aquatic rhabdoviruses spring viremia of carp virus (SVCV) and infectious hematopoietic necrosis virus (IHNV) were explored, and the results demonstrated that M3 evidently lessened the production of CPE induced by SVCV and IHNV, and inhibited the proliferation of SVCV and IHNV in EPC cells in a dose-dependent manner, with IC 50 of 2.92 μM and 2.78 μM, respectively. To sum up, M3 exhibits a high antiviral activity against MSRV, and is expected to be developed into antiviral specific drugs against rhabdoviruses in aquaculture.
研究领域:
水产养殖学
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病毒学
·
药理学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Xiaojiao Zhu,Gang Liu,Yingcui Bu,Jie Zhang,Lianke Wang,Yupeng Tian,Jianhua Yu,Zhichao Wu,Hongping Zhou
Cell viability is greatly affected by external stimulus eliciting correlated dynamical physiological processes for cells to choose survival or death. A few fluorescent probes have been designed to detect whether the cell is in survival state or apoptotic state, but monitoring the regulation process of the cell undergoing survival to death remains a long-standing challenge. Herein, we highlight the in situ monitor of mitochondria regulating the cell viability by the RNA-specific fluorescent photosensitizer L. At normal conditions, L anchored mitochondria and interacted with mito-RNA to light up the mitochondria with red fluorescence. With external light stimulus, L generated reactive oxide species (ROS) and cause damage to mitochondria, which activated mitochondrial autophagy to prevent death, during which the red fluorescence of L witnessed dynamical distribution in accordance with the evolution of vacuole structures containing damaged mitochondria into autophagosomes. However, with ROS continuously increasing, the mitochondrial apoptosis was eventually commenced and L with red fluorescent was gradually accumulated in the nucleoli, indicating the programmed cell death. This work demonstrated how the delicate balance between survival and death are regulated by mitochondria.
研究领域:
细胞生物学
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分子生物学
·
生物医学工程
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DOI
PubMed
高影响因子
NEUROSCIENCE LETTERS
·
2018
·
IF 2.159
Dongling Shi,Teng He,Weijian Tang,Heyangzi Li,Chao Wang,Mingzhi Zheng,Jue Hu,Xinghui Song,Yuemin Ding,Ying-ying Chen,Yueliang Shen,Hongfeng Jin,Lin-lin Wang
Neuronal death and organization degeneration can happen inordinately after spinal cord injury (SCI), which lead to nerve dysfunction. We aimed to determine whether local application of a cell permeable calpain I inhibitor (MDL28170) can promote SCI recovery by increasing neuronal cell viability. MDL28170-loaded polycaprolactone (PCL) film was fabricated. Scanning electron microscopy showed the surface of PCL film was smooth with holes (diameter at μM level). The PCL film was non-toxic, biological compatibility, and had good neuron adhension and slow release characteristic. MDL28170 increased VSC4.1 motor neurons’ viability under tunicamycin (an endoplasmic reticulum stress) induced injury. In a traumatic SCI rat model, MDL28170-loaded PCL film reduced the area of lesion cavity, and promoted recovery of locomotor behavior. Moreover, the expression of GAP-43 was upregulated after MDL28170-loaded PCL film treatment. Thus, our findings demonstrated that localized delivery of MDL28170 could promote SCI recovery by inhibiting endoplasmic reticulum stress, preserving survival of the motor neurons, which may point out a promising therapeutic target for treating SCI patient.
研究领域:
神经科学
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生物医学工程
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药理学
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DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2021
·
IF 5.565
Tingting Fang,Zuotao Zhang,Hui Wang,Matt Rogers,Qijia Cui
The decay and distribution of bacterial pathogens in water is an important information for the health risk assessment to guide water safety management, and suspended algae might affect bacterial pathogens in water. This study established microcosms to investigate the effects of algae-related factors on the representative indicators and opportunistic pathogen species in water. We found that suspended algae increased the persistence of targeted species by 1-2 orders of magnitude of concentrations compared to microcosms without algae; and the effect of algae on microbial survival was affected by water nutrient levels (i.e., carbon, nitrogen and phosphorus), as the increased microbial persistence were correlated to the increased algae concentrations with more nutrient supplies. Moreover, decay and distribution profiles of representative species were determined. The three opportunistic pathogen species ( Pseudomonas aeruginosa, Aeromonas hydrophila and Staphylococcus aureus ) showed lower decay rates (0.82–0.98/day, 0.76–0.98/day, 0.63–0.87/day) largely affected by algae-related factors, while the enteric species ( Escherichia coli and Enterococcus faecalis ) had higher decay rates (0.94–1.31/day, 0.89–1.21/day) with little association with algae, indicating the propensity for attachment to algae is an important parameter in microbial fate. Together results suggest suspended algae played an evident role in the decay and distribution of bacterial pathogens, providing important implications regarding microbial safety in recreational water.
研究领域:
环境科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Hongxia Gao,Li Ma,Wenxuan Sun,David Julian McClements,Ce Cheng,Huaying Zeng,Liqiang Zou,Wei Liu
Probiotics have many beneficial physiological effects, such as enhancing immunity and regulating intestinal microflora. However, the highly acidic conditions in the stomach and the high bile acid concentration in the small intestine pose challenges to the maintenance of probiotic activity after ingestion. In this study, we examined the possibility of increasing the viability of probiotics under gastrointestinal conditions by encapsulating them within oil-in-water high internal phase emulsions (HIPEs). These emulsions were prepared using whey protein isolate (WPI) and pectin, which were gelled by adding GDL and calcium to form double network HIPE gels. The gel strength, stability, and shear viscosity of the gelled HIPEs increased as the pectin concentration was raised from 0 to 2%. Cryo-SEM analysis showed that the pectin formed a dense hydrogel network in the aqueous phase surrounding the oil droplets in the HIPEs. Probiotics were encapsulated within the gelled HIPEs by dispersing a probiotic powder in the oil phase. Confocal microscopy indicated that the probiotics were mostly distributed in the water, and interfacial phases, with only a few of the probiotics remaining in the oil phase. Encapsulation of the probiotics in the HIPEs increased the number of live cells remaining after a heat treatment and after passage through a simulated gastrointestinal model. The incorporation of pectin into the HIPEs increased the viability of the probiotics, especially after exposure to the gastric phase. This study highlights the potential of HIPEs in preserving probiotic activity, which may be useful for the development of more effective functional foods.
研究领域:
食品科学
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微生物学
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DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 21
·3468
·
IF 5.561
Shumao Cui,Ziyi Pan,Sheng Wu,Bingyong Mao,Xin Tang,Qiuxiang Zhang,Hao Zhang,Jianxin Zhao
The influence of surface substance production on the freeze-drying survival ofLactobacillus caseiand methods to control the surface substances during fermentation were studied. The bacteria were treated with hypertonicity combined with ultrasound, and the survival rate was determined. The optimal conditions for removing surface substance without harming the bacteria were 81 w/18 min. The surface substances provided a protective effect on the lyophilization of the bacteria without protectants. However, in the presence of protectants, excessive surface substances reduced the protective effect of the optimum protectant alginate to 39.69 ± 1.27%. Finally, the amount of surface substances and lyophilized survival rate of collected bacteria were determined by adding EDTA during fermentation and regulating fermentation conditions, such as the carbon source, carbon-to-nitrogen ratio, and pH. The highest survival rate was 85.79 ± 3.29%, which was achieved when the amount of surface substances was (2.82 ± 0.55) × 10−11mg/CFU. Therefore, the production of surface substances by the bacteria could be reduced by modifying the fermentation stage, which has significance in the improvement of the lyophilization survival rate ofL. caseiand the number of live bacteria per unit mass ofL. caseiin the lyophilized preparation.Keywords:lyophilization survival rate;surface substance;Lactobacillus casei;fermentation conditions
研究领域:
微生物学
·
食品科学
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DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2019
·
IF 4.259
Chi Xiaohui,Berglund Björn,Zou Huiyun,Zheng Beiwen,Börjesson Stefan,Ji Xiang,Ottoson Jakob,Lundborg Cecilia Stålsby,Li Xuewen,Nilsson Lennart E.
Klebsiella pneumoniae is a gram-negative, opportunistic pathogen, and a common cause of healthcare-associated infections such as pneumonia, septicemia, and urinary tract infection. The purpose of this study was to survey the occurrence of and characterize K. pneumoniae in different environmental sources in a rural area of Shandong province, China. Two hundred and thirty-one samples from different environmental sources in 12 villages were screened for extended-spectrum β-lactamase-(ESBL)-producing K. pneumoniae, and 14 (6%) samples were positive. All isolates were multidrug-resistant and a few of them belonged to clinically relevant strains which are known to cause hospital outbreaks worldwide. Serotypes, virulence genes, serum survival, and phagocytosis survival were analyzed and the results showed the presence of virulence factors associated with highly virulent clones and a high degree of phagocytosis survivability, indicating the potential virulence of these isolates. These results emphasize the need for further studies designed to elucidate the role of the environment in transmission and dissemination of ESBL-producing K. pneumoniae and the potential risk posed to human and environmental health.
研究领域:
微生物学
·
传染病学
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环境卫生
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公共卫生
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-22
·
IF 12.6
Yang Ningning,Hua Rongrong,Lai Yingying,Zhu Peijun,Ding Jian,Ma Xianhui,Yu Gaoxiang,Xia Yiheng,Liang Chao,Gao Weiyang,Wang Zhouguang,Zhang Hongyu,Yang Liangliang,Zhou Kailiang,Ge Lu
In the field of large-area trauma flap transplantation, preventing avascular necrosis remains a critical challenge. Key mechanisms for improving flap viability include angiogenesis promotion, oxidative stress inhibition, and cell death prevention. Recently, two-dimensional ultrathin Ti 3 C 2 T X (MXene) nanosheets have gained attention for their potential contributions to these processes, though MXene’s physiological impact on flap survival had not been previously investigated. This study is the first to confirm MXene's biological effects on the ischaemic microenvironment post-skin flap transplantation. Findings indicated that MXene significantly decreased the necrotic area in ischaemic flaps (37.96% ± 2.00%), with reductions of 30.40% ± 1.86% at 1 mg/mL and 20.19% ± 2.11% at 2 mg/mL in a concentration-dependent manner. Mechanistically, MXene facilitated in situ angiogenesis, mitigated oxidative stress, suppressed pro-inflammatory pyroptosis, and activated the PI3K-Akt pathway, particularly influencing vascular endothelial cells. Comparative transcriptome analysis of skin tissues with and without MXene treatment provided additional evidence, highlighting mechanisms such as pro-inflammatory pyroptosis, ROS metabolic processes, endothelial cell proliferation regulation, and PI3K-Akt signaling pathway activation. Overall, MXene demonstrated biological activity, effectively promoting ischaemic flaps survival and presenting a novel strategy for addressing ischaemic necrosis in skin flaps. Graphical abstract
研究领域:
医学
·
生物材料学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Cell and Developmental Biology
·
2025
·
IF 4.3
Weijie Li,Xi Yang,Wenyan Bi,Liyong Song,Baolin Liu
Developing bioartificial liver and hepatocyte transplantation technology causes increasing hepatocyte cell demand. Effective long-term hepatocyte cell preservation methods are necessary to promote. Progressive cooling is a key preservation technology for cell banks. However, the cell solution needs to be supercooled in a slow freezing process. The high degree of supercooling possibly induces uncontrollable intracellular ice formation. This work designs an ultrasonic ice-seeding system for L-02 hepatocyte cell preservation, reducing supercooling and improving cell survival rate. The effect of ultrasonic intensities on the hepatocyte’s survival rate was investigated and optimized. The results prove the calorimetric method can efficiently measure the ultrasonic intensity dissipated in the hepatocyte cell preservation solution. When the ultrasonic intensity is 0.0329 W/cm 2 ∼ 0.4316 W/cm 2 , the hepatocyte survival rate is over 90%. There is no significant difference between experiment groups (p < 0.05) when the ultrasonic intensity is larger than 0.4316 W/cm 2 . The hepatocyte cell survival rate reduced significantly with the increase of ultrasonic intensity. The 7-day hepatic function indicator experiment results indicate that the ultrasonic ice seeding has the weakest impact on hepatocyte cells in the four groups. The secretion of urea, albumin and glucose proved that ultrasonic ice seeding technology does not affect cell secretion and has an enormous advantage in cryopreservation. It can be widely applied to cell freezing fields.
研究领域:
生物技术
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医学工程
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Kun Feng,Ru-meng Huang,Rui-qing Wu,Yun-shan Wei,Min-hua Zong,Robert J. Linhardt,Hong Wu
To improve the survivability of probiotics under the harsh conditions, a novel double-layered vehicle, which was developed by a one-step coaxial electrospinning procedure, was here used to encapsulate the probiotics. The morphology characterization analysis revealed that the electrospun fiber had a beaded morphology and core-shell structure. Probiotic cells were successfully encapsulated in the fibers (10 7 CFU/mg) and exhibited an oriented distribution along the fiber. Additionally, the encapsulation of core-shell fiber mat enhanced the tolerance of probiotic cells to simulated gastrointestinal conditions and no significant loss of viability was found (p > 0.05). Besides that, the encapsulated cells exhibited better thermal stability under heat moisture treatment, lower loss of viability (0.32 log CFU/mL) was occurred when compared with the free cells or encapsulated cells in uniaxial fiber mat. In conclusion, this double-layered vehicle presents a great potential in probiotic encapsulation and improving their resistant ability to the harsh conditions.
研究领域:
食品科学
·
生物工程
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）