INTERNATIONAL DAIRY JOURNAL
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2024
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IF 3.1
Yiwei Xu
The presence of Heyndrickxia sporothermodurans is a widespread concern in dairy industry due to its highly thermal-resistant spores. The "Cleaning-in-place" (CIP) system is the primary method used by dairy industry to control them. But the food safety-related properties of H. sporothermodurans remain poorly understood. Thus, this study proposes to determine the growth characteristics and sporulation of H. sporothermodurans in different matrices, as well as the efficacy of various disinfectants against its biofilm and spore. Therefore, the growth of vegetative cell and spore germination in rich media (brain heart infusion, BHI) and ultra-high temperature (UHT) milk for at least 360 h was investigated in this research. In addition, the biofilms of different maturity induced on stainless steel surfaces were sanitized simulating a CIP procedure, using disinfectants (200 mg/mL peracetic acid, 1.5% sodium hydroxide and 1% nitric acid). Scanning electronic microscopy (SEM) was used as a complementary technique to observe the conditioning of the stainless steel surface after 10 d. Subsequently, the effects of these sanitation methods against the spore were performed in 96-well plates. The observation of growth behavior revealed that the vegetative cells and spore cells may be interconverted in a nutrient-rich environment, resulting in a balanced mode in which spores account for approximately 0.1% of the total cells. This could be used to anticipate the number of contaminated bacteria in a UHT plant environment. The sterilization efficiency tests showed that these sanitation methods were efficient against biofilms but not against spores. Therefore, new inactivation strategies against spores should be proposed and should focus on spores adhesion and the biofilms induced by spores.
研究领域:
微生物学
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食品安全
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乳品科学
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DOI
高影响因子
Genes
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2019
·Vol. 10
·期 6
·412
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IF 3.331
Zhangxun Wang,Hong Zhu,Yuran Cheng,Yuanyuan Jiang,Yuandong Li,Bo Huang
The polyubiquitin gene is a highly conserved open reading frame that encodes different numbers of tandem ubiquitin repeats from different species, which play important roles in different biological processes.Metarhizium robertsiiis a fungal entomopathogen that is widely applied in the biological control of pest insects. However, it is unclear whether the polyubiquitin gene is required for fungal development, stress tolerance, and virulence in the entomopathogenic fungus. In the present study, the polyubiquitin gene (MrUBI4, MAA_02160) was functionally characterized via gene deletion inM. robertsii.Compared to the control strains, theMrUBI4deletion mutant showed delayed conidial germination and significantly decreased conidial yields (39% of the wild-type 14 days post-incubation). Correspondingly, the transcript levels of several genes from the central regulatory pathways associated with conidiation, includingbrlA,abaA,andwetA, were significantly downregulated, which indicated thatMrUBI4played an important role in asexual sporulation. Deletion ofMrUBI4especially resulted in increased sensitivity to ultraviolet (UV) and heat-shock stress based on conidial germination analysis between mutant and control strains. The significant increase in sensitivity to heat-shock was accompanied with reduced transcript levels of genes related to heat-shock protein (hsp), trehalose, and mannitol accumulation (tps,tpp,nth,andmpd) in theMrUBI4deletion mutant. Deletion ofMrUBI4has no effect on fungal virulence. Altogether,MrUBI4is involved in the regulation of conidiation, conidial germination, UV stress, and heat-shock response inM. robertsii.Keywords:Metarhizium robertsii;polyubiquitin;conidiation;conidial germination;stress tolerance
研究领域:
真菌学
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分子生物学
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植物保护
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Shunyu Xiang,Meijun Chen,Xingyi Luo,Shicai Zhang,Yang Shen,Xingya Chen,Xiaofeng Zhang,Jing Wang,Huan Tang,Jin Huang,Xianchao Sun
Oomycetes are devastating plant pathogens causing major crop losses, with spores as key infection sources. Inhibiting asexual reproduction, especially sporangium formation and spore release, is crucial for disease prevention. Zn2+ has shown potential in inhibiting oomycete reproduction, but excessive concentrations can cause cytotoxicity and environmental risks. To address this, we used polydopamine (PDA) to complex Zn2+ and form a PDA@Zn2+ coating on cellulose nanocrystals (CNCs) through hydrogen bonding. This ionic nanopesticide (CNC@PDA@Zn2+) enhances effectiveness against oomycetes while reducing dosage and improving biosafety. Bioexperimental results indicate that CNC@PDA@Zn2+ significantly inhibits sporangium formation and spore release from Phytophthora capsici (P. capsici) by suppressing the expression of key sporulation genes (PcATP4, cdc, and G-protein), disrupting spore cell membranes, and altering organelle structures. In vivo, it reduces P. capsici infection on pepper leaves, even in the presence of mycelium. This study highlights CNC@PDA@Zn2+ as a promising biobased nanomaterial for sustainable crop protection.
研究领域:
植物病理学
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纳米生物学
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农业科学
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产品关联
DOI
PubMed
高影响因子
Journal of Integrative Agriculture
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2023
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IF 4.8
Jia-nan SUN,Gao-yue SI,Hong-yi LIU,Ya-qian LI,Xin-hua WANG,Jie CHEN
Excessive use of organophosphate pesticides (OP), such as dichlorvos, in farming system poses a threat to human health through potential contamination of environment. To date, biodegradation has been prospected most promising approach to eliminate environmental OP residues. Trichoderma species as a biological control microorganism is often exposed to the chemical pesticides applied in environments, so it is necessary to understand the mechanism of degradation of dichlorvos by Trichoderma . In this study, dichlorvos significantly inhibited the growth, sporulation and pigmentation of T. atroviride T23, and the dichlorvos degradation activity of T23 required the initial induction effect of dichlorvos and the culture conditions, including the nutrient and pH values of the medium. Various changed primary and secondary metabolites released from T23 in the presence of dichlorvos were speculated as the energy and antioxidants for the strain itself to tolerate dichlorvos stress. The results showed that T23 could produce a series of enzymes, especially the intracellular enzymes, to degrade dichlorvos. The activities of the intracellular enzyme generated by T23 were differentially changed along time course and especially relied on initial dichlorvos concentration, ammonium sulfate and phosphate added in the medium. In conclusion, some dichlorvos-induced chemical degradation related enzymes of T23 were proved to be involved in the degradation of dichlorvos.
研究领域:
环境科学
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微生物学
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生物技术
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农药科学
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DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
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2023
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IF 7
Jianheng Guo,Yue Xu,Sunyan Liang,Zhengkun Zhou,Chunmei Zhang,Kui Li,Xue Peng,Sheng Qin,Ke Xing
Botrytis cinerea is a necrotrophic fungal pathogen that causes gray mold disease in more than 1400 plant species. The biocontrol effect of microbial volatile compounds on postharvest diseases has become a research hotspot. This study revealed the biocontrol effects of volatiles released by Bacillus tequilensis XK29 on B. cinerea in vitro and in vivo . The results showed that the strain XK29 volatiles inhibited the mycelial growth of B. cinerea . The inhibition effect of strain XK29 volatiles against B. cinerea was increased by optimizing the wheat seeds weight, suspension concentration and inoculation volume of strain XK29, and the pre-culture time. The volatiles of strain XK29 affected the mycelial development, the formation of conidia , and the metabolic activity of B. cinerea . Selected individual monomers and artificial mixtures showed strong antifungal activity against B. cinerea. The volatiles of strain XK29 reduced the lesion diameter and disease severity of cherry tomatoes after inoculation with B. cinerea. These results indicate that the fumigation with B. tequilensis XK29 volatiles is a promising approach to protect cherry tomatoes from gray mold disease. This is also the first study to prove the antifungal effect of B. tequilensis on B. cinerea and its biocontrol effect on postharvest cherry tomatoes.
研究领域:
植物病理学
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微生物学
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食品科学
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产品关联
DOI
高影响因子
Food Bioscience
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2024
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IF 4.8
Deguang Sun,Xueyou Zhou,Desheng Chen,Fengjie Liu,Bo Zhang,Xue Sun,Nianjun Xu
A sustainable food supply is crucial for addressing the food crisis resulting from the increasing global population. Seaweeds are promising food candidates because of their nutritional value and favorable growing conditions, as they do not occupy arable land or require fresh water. Gracilariopsis lemaneiformis (Rhodophyta), known for its ability to produce agar, is attracting increasing attention in the food industry. To develop new G. lemaneiformis germplasms, the four stages tetrasporophyte development and related molecular mechanisms were previously studied, but the metabolite changes that occur during the reproductive process remain unknown. In this study, we employed comprehensive metabolomics technology to examine changes in metabolites during the reproductive process of G. lemaneiformis , and a total of 817 metabolites were found, including 355 differentially accumulated metabolites (DAMs). The results revealed that G. lemaneiformis fulfills its energy requirements during reproduction by increasing glycolysis and TCA cycle flux, while it meets its material requirements by activating amino acid and nucleotide metabolism. Additionally, a significant accumulation of biogenic amines produced by the shikimate pathway may promote the formation of tetraspores, and a combination of flavonoids and phenolic acids effectively preserved the balance of the antioxidant system during the reproductive process of G. lemaneiformis . In this research, we analyzed changes in metabolites during the transition from the nutritional stage to the reproductive stage of G. lemaneiformis , revealing the potential relationship between tetraspore formation and metabolites and providing a theoretical basis for the cultivation of new germplasms of G. lemaneiformis .
研究领域:
海洋生物学
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代谢组学
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植物繁殖
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食品科学
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DOI
高影响因子
JOURNAL OF VETERINARY MEDICAL SCIENCE
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2024
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IF 1.1
Nianyuan CHEN,Qingxiu CAI,Shujing WANG,Qingyang SONG,Ying XIE,Huijuan SHI,Hongmei LI,Xiaomin ZHAO,Ningning ZHAO,Xiao ZHANG
Protozoan parasites such as Eimeria tenella and Toxoplasma gondii pose significant health challenges in livestock and humans. The limited treatment options and rising drug resistance underscore the urgent need for new therapies. This study investigates myrcene, a monoterpene hydrocarbon classified for its antiprotozoal potential against E. tenella and T. gondii infections. Initially, we examined its effect on the sporulation process of E. tenella oocysts in vitro and its anti-E. tenella activity in vivo. Myrcene significantly reduced the sporulation rate of E. tenella oocysts at 3 and 4 mg/kg. In vivo experiments demonstrated that treatment with 4 mg/kg myrcene significantly reduced E. tenella load and oocyst output, as well as cecal lesion and weight loss caused by E. tenella infection, showing moderate anti-E. tenella activity, with an Anticoccidial Index (ACI) of 161.4. Furthermore, we investigated the anti-T. gondii activity of myrcene both in vitro and in vivo. In vitro studies showed that treatment with myrcene effectively inhibited the invasion rate and intracellular proliferation ability of T. gondii tachyzoite in DF-1 cells in a dose-dependent manner. In vivo administration prolonged the survival time in T. gondii-infected mice, suggesting notable protective effects. Additionally, it mitigated T. gondii-induced hepatosplenic toxicity by reducing parasite load in the liver and spleen, and ameliorating liver function as evidenced by decreased serum transaminase levels. In conclusion, the findings demonstrate promising anti-E. tenella and anti-T. gondii activity exhibited by myrcene warranting further exploration into its mechanisms and potential therapeutic applications.
研究领域:
兽医学
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寄生虫学
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药理学
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DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
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2022
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IF 9.224
Ze-Wang Guo,Zi-Fu Ni,Min-Hua Zong,Wen-Yong Lou
Consolidated bioprocessing (CBP) has emerged as a promising strategy for green synthesis of 2,3-butanediol (BDO) from Jerusalem artichoke tubers (JAT). Here, modular metabolic engineering of Bacillus licheniformis MW3 was implemented, in which the overall BDO synthesis process was divided into four modules (inulin hydrolysis, sporulation elimination, carbon flux redistribution, and enantiopure meso-BDO synthesis). First, endo-inulinase was introduced to boost inulin utilization. Second, sporulation was blocked by spoIIE knockout, thus reducing the leakage risk of the genetically engineered strain from the fermentation plant. Third, more carbon flux was shunted into BDO production via exchanging the alsS start codon, blocking acetoin catabolism, and competing pathways. Finally, enantiopure meso-BDO was synthesized by gdh knockout. Encouragingly, the final engineered strain, MWO-8, produced meso-BDO with 99.3% purity at a titer, productivity, and yield of 82.51 g/L, 1.38 g/L/h, and 0.439 g/g, respectively. To our knowledge, this is the highest titer of BDO from JAT by CBP so far. Collectively, this work provides a highly promising alternative approach for sustainable and cost-effective production of BDO.
研究领域:
生物技术
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代谢工程
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生化工程
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微生物学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）