Pregnane X receptor attenuates gold nanoparticles’ toxicity through accelerating zebrafish embryo hatching
It is well known that fish embryos are vulnerable to waterborne nanoparticles (NPs), with delayed hatching being the most common and sensitive endpoint. Up-regulation of hatching enzymes has been believed to be an important detoxification mechanism for NPs, but the inner mechanism for such phenomena has been seldom investigated. This study aimed to investigate the role of pregnane X receptor (Pxr) in maintaining the robustness of embryo hatching after treatment with gold nanoparticles (AuNPs, 4 and 82 nm). For this purpose, embryos from mating of 6-month-old wild-type (WT) AB strain zebrafish ( Danio rerio , 3∼4-cm-length) were treated with AuNPs since 4 h post-fertilization (hpf). It was found that both AuNPs significantly inhibited embryo hatching after 52-h treatment, with Au-4 being more toxic at the same mass concentrations. At non-toxic concentrations and median effective concentrations (EC 50 ) of delayed hatching, both AuNPs induced the mRNA expression of HEs and Pxr at 48 hpf, and Au-4 seemed to be more effective. The induction extents of HEs by AuNPs decreased when Pxr was knocked out or inhibited, indicating the role of Pxr in such process. Additionally, knockout/inhibition of Pxr significantly delayed the hatching of embryos at 56 hpf, and activation of Pxr accelerated the process at moderate concentrations. Such phenomena correlated well with the alterations in the mRNA expression and activities of HEs, indicating a fact that AuNPs activated Pxr and up-regulated HEs, which helped the detoxification of AuNPs. RNA-sequencing analysis of WT and pxr -deficient embryos at 24 hpf confirmed the alteration of he1.1&1.2 . In addition, Pxr influenced mRNA encoding muscle development (muscle system process and striated muscle tissue development) and energy metabolism (carbohydrate metabolic process and ATP metabolic process), which were related to the motility of embryos and determined the hatching speed. Such function was confirmed by the reduced locomotor activity of pxr-deficient larvae at 120 hpf. Overall, these results suggested a novel role of Pxr in promoting the hatching of zebrafish embryos, which contributed to the detoxification of AuNPs.
危险品化学品经营许可证（带存储）