CHEMOSPHERE
·
2020
·
IF 5.778
Shangqi Ge,Yize Pan,Lingwei Zheng,Xinyu Xie
Stabilization/solidification (S/S) has been studied since 1950s and widely used for the treatment of potentially toxic elements (PTEs). The coexistence of organic matter (OM) and PTEs can cause a very complicated mechanism for cement-based S/S applications and bring challenges from both scientific and engineering perspectives. To fill in the knowledge gap, this paper investigates for the first time the effects on S/S characteristics of OM components and incubation, which are the two main factors that result in the inconsistency in the leaching characteristics from the available studies. OM samples with different components (humic acid (HA) and fulvic acid (FA)) and contents were mixed into lead-contaminated soil and incubated for different durations of up to 90 days. The experimental results show that the strength of stabilized soils increases with increasing incubation duration and the lead leaching concentration of stabilized soils is decreased by 60.7%–83.6% from zero to 90 days. The lead leaching concentration of the HA group, which is 144.0% higher with no incubation than the non-OM group, becomes 58.3% lower with 90 days of incubation. The leaching concentration of the FA group remains much higher than those of the other groups. Finally, a competing mechanism of HA—weakening cement hydration reactions and stabilizing lead with a critical incubation duration of 14 days—is proposed, together with a cooperating mechanism of FA—weakening cement hydration reactions and releasing lead.
研究领域:
环境科学
·
土木工程
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DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2020
·
IF 2.463
Xiao-Qi Jia,Yi Li,Chun-Xia Zhang,Yan-Cai Gao,Yuqing Wu
Due to the importance of ethanol-water solutions in many fields the different supramolecular clusters involved in this binary system is studied in detail by using fluorescence spectroscopy , at different concentration and incubation time after mixing. Two-dimensional (2D) correlation analysis is performed on the concentration-dependent mixtures (10–100%) at each incubation time, which find that the correlation intensity of emission peak at 330 nm for (H 2 O) m (EtOH) n not only related to the concentration but also gradually increase with the incubation time. Such results indicate that the cluster of (H 2 O) m (EtOH) n is the most stable one because it tends to be formed with longer incubation time. In addition, the correlation results indicate that at low concentration range of 10–45%, the dominated cluster is (H 2 O) m (EtOH) corresponding to a peak at 373 nm; while at high concentration range of 80–100%, the main cluster is (H 2 O)(EtOH) n corresponding to an emission peak at 308 nm; however, the most stable cluster of (H 2 O) m (EtOH) n , corresponding to the peak at 330 nm, not only predominate at the concentration range of 50–75% but also exist in all mixture at longer incubation time. Therefore, the present study provides a new approach to clarify the different supramolecular clusters in ethanol-water mixtures, which would be applied to other binary solution system.
研究领域:
物理化学
·
光谱学
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产品关联
DOI
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
·
2021
·Vol. 110
·期 5
·1034-1043
·
IF 3.368
Tao Zhang,Dekun Zhang,Hongtao Liu,Kai Chen
The abrasive debris produced by wear test of artificial joints in vitro is encapsulated by proteins in serum lubricants, which hinder the characterization of debris analysis. One of the key issues of isolating wear debris from serum is degrading the proteins wrapping the wear debris. In this article, the proteins in calf serum were degraded by a strong alkali, a strong acid, and an enzyme. The residual concentration of proteins in calf serum was detected by UV absorption. Quantitative analysis of protein degradation and the protein degradation rate was proposed, following treatment with different degradation reagents and different incubation times. The results showed that when 10 mL of 25% volume calf serum was added with 40 mL of NaOH and incubated at 65°C for 24 h, the protein degradation rate reached a maximum of 95.52%. The protein degradation rate in the solution ranged from 31.86% to 71.64% when a different volume of 37% HCl was added and incubated at 60°C. The highest protein degradation rate was 94.98% in the protease degradation solution. When the protein degradation rate is less than 70%, the particles were coated by protein. When the protein degradation rate was more than 95%, there was no protein coating around the particles. The three protein degradation methods have different processes and protein degradation rates. A suitable method for protein degradation can be selected according to these practical applications.
研究领域:
生物医学工程
·
材料科学
·
蛋白质化学
·
磨损分析
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Huayan Yang,Sijia Lu,Shengkun Wang,Lihong Liu,Bo Zhu,Shaoning Yu,Shouning Yang,Junbiao Chang
When nanoparticles (NPs) come into contact with bioenvironments, a protein corona forms on the NP surface. Previous reports showed that the constituents of the corona change with time. However, how different protein corona compositions influence cells, especially immune cells, has received less attention. Macrophages are important immune cells that can be polarized into a pro-inflammatory (M1) or anti-inflammatory (M2) phenotype. In this study, AuNPs were incubated with human plasma for different periods to obtain time-related AuNP-coronas, and the influences of time-related AuNP-coronas on macrophage polarization were investigated. The macrophage morphology, biomarkers, cytokine secretion studies show that the pristine AuNPs and 4 h-AuNP-corona induced macrophage cells into M2 phenotype, while the co-incubation of 12 h-AuNP-corona and macrophage cells result in M1 phenotype. Further proteomic analysis showed that the compositions of protein corona were changing constantly after AuNPs contacted with plasma. When the incubation time increased to 12 h, the immune proteins in protein corona were increased significantly, which play a key role in modulation of the different macrophages polarization. Our findings demonstrated that plasma incubation time is an important parameter that needs to be taken into account in the study of nano-immune interactions and safe use of NPs in biological systems. Moreover, our finding can be a new efficient strategy for activating inflammatory or anti-inflammatory in medical treatment.
研究领域:
纳米技术
·
免疫学
·
生物医学工程
·
蛋白质组学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2015
·Vol. 182
·期 9
·1677-1683
·
IF 3.741
Zhang Yuanfu,Li Rui,Xue Qingwang,Li Haibo,Liu Jifeng
A branched polyethyleneimine was used to functionalize gold nanoparticles (AuNPs). It is found that copper ions rapidly induce the aggregation of such AuNPs, this resulting in a color change from red to purple. The effect can be exploited for visual (bare-eye) detection or instrumental quantification of Cu(II) by measuring the ratio of absorbances at 650 and 520 nm at pH 4 and after a 7-min incubation time. The method works in the 0.1 to 10 μM Cu(II) concentration range and with a 30 nM limit of detection. It exhibits fairly good selectivity over other common metal ions. The standard addition method was successfully applied to the determination of Cu(II) in (spiked) tap and lake waters.
A simple and selective colorimetric assay for Cu(II) is presented. It is based on Cu(II)-induced aggregation of polyamine-functionalized gold nanoparticles.
研究领域:
分析化学
·
环境科学
·
纳米技术
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Ye Hu,Yajiao Huang,Yanying Wang,Chunya Li,WingLeung Wong,Xiaoxue Ye,Dong Sun
ZnAgInS quaternary quantum dots were prepared using glutathione as the capped reagent. Gold nanoparticles (GNPs) were integrated with ZnAgInS QDs to provide a GNPs/ZnAgInS QDs nanocomposite. The morphological image, component and crystal structure of GNPs/ZnAgInS QDs were characterized by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD). A glassy carbon electrode surface was coated with GNPs/ZnAgInS QDs nanocomposites to construct an interface for immobilizing the antibody of hepatitis B virus surface antigen (anti-HBsAg). By employing GNPs/ZnAgInS QDs as a photoactive element, a photoelectrochemical immunosensor for hepatitis B virus surface antigen (HBsAg) was developed. The results indicate that gold nanoparticles can dramatically enhance the photocurrent response of ZnAgInS QDs and thus improving the sensing performances of the immunosensor. The experimental conditions including incubation time , incubation temperature , and ascorbic acid concentration were optimized. The relative photocurrent decline [ R i = Δ I / I 0 = ( I 0 − I )/ I 0 ] shows a linear relationship to the logarithm of HBsAg concentration [lg( c , ng mL −1 )] in the range from 0.005 to 30 ng mL −1 . A detection limit of 0.5 pg mL −1 was obtained. The immunosensor shows excellent sensitivity, selectivity , stability and reproducibility. The HBsAg concentrations in clinical serum samples were also accurately determined with this new photoelectrochemical immunosensor.
研究领域:
纳米技术
·
分析化学
·
生物化学
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
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