SENSORS
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2019
·Vol. 19
·期 5
·1203
·
IF 3.031
Nannan Shen,Haijun Xu,Weichen Zhao,Yongmei Zhao,Xin Zhang
Glucose concentration is an important physiological index, therefore methods for sensitive detection of glucose are important. In this study, Au foam was prepared by electrodeposition with a dynamic gas template on an Au nanoparticle/Si substrate. The Au foam showed ultrasensitivity, high selectivity, and long-term stability in the quantitative detection of glucose. The foam was used as an electrode, and the amperometric response indicated excellent catalytic activity in glucose oxidation, with a linear response across the concentration range 0.5 μM to 12 mM, and a limit of detection of 0.14 μM. High selectivity for interfering molecules at six times the normal level and long-term stability for 30 days were obtained. The results for electrochemical detection with Au foam of glucose in human serum were consistent with those obtained with a sensor based on surface-enhanced Raman spectroscopy and a commercial sensor. This proves that this method can be used with real samples. These results show that Au foam has great potential for use as a non-enzymatic glucose sensor.Keywords:Au foam;bubble-templated electrodeposition;non-enzymatic glucose sensor;amperometric response
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2023
·
IF 2.9
Bo Yang,Huan Li,Cuijie Nong,Xiaokun Li,Suxiang Feng
In this work, a label-free electrochemical immunosensor using tin sulfide/nickel cobalt metal-organic frameworks (SnS 2 /NiCo MOFs) was established for the sensitive detection of cortisol. First, SnS 2 /NiCo MOFs were synthesized by doping SnS 2 with NiCo MOF nanocubes by a hydrothermal method . Then, gold nanoparticles (AuNPs) were grown in situ on SnS 2 /NiCo MOFs for electrochemical detection . The use of SnS 2 /NiCo MOFs promoted the electron transfer rate of AuNPs and enhanced the electrochemical sensing performance of AuNPs@SnS 2 /NiCo MOFs-modified electrodes. The large specific surface area of AuNPs@SnS 2 /NiCo MOFs provides more active sites for antibody loading. After the prepared immunosensor was incubated with the target analyte, cortisol, the electron transfer impedance increased and the amperometric response decreased, thus establishing a highly sensitive immunosensing method. The sensor had a linear range of 100 fg/mL to 100 ng/mL and a low detection limit of 29 fg/mL. The sensor showed good accuracy and practicability and could be used for the determination of cortisol in saliva.
研究领域:
电化学
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分析化学
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纳米技术
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生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
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2020
·Vol. 24
·期 3
·583-590
·
IF 2.646
Yu Zhipeng,Kong Chuncai,Lv Jian,Ma Bo,Zhang Xiaojing,Yang Zhimao
Designing highly active material to fabricate high-performance glucose sensor was great importance for diabetes treatment. In this work, we develop a novel nanocomposite composed of multilevel nanostructure with ultrathin Cu x O nanoflakes anchored on Cu 2 O nanowires, directly on Cu foil and its application as non-enzymatic glucose sensor. Due to more active sites endowed by the ultrathin Cu x O nanoflakes, the as-prepared Cu x O nanoflakes/Cu 2 O nanowires@Cu nanoarray exhibit high sensitivity (3.59 mA mM −1 cm −2 ), low detection limit (0.69 μM), high selectivity, fast amperometric response (~ 2 s) and excellent reliability in human serum. And the sensitivity of ultrathin Cu x O nanoflakes/Cu 2 O nanowires@Cu nanoarray was 1.9-fold than that of Cu 2 O nanowires@Cu nanoarray. Such novel multilevel nanoarray synthesized by convenient method provides a facile way to design metal oxide nanomaterial for promising non-enzymatic glucose sensor.
研究领域:
材料科学
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纳米技术
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分析化学
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2015
·Vol. 182
·期 11
·1949-1956
·
IF 3.741
Wang Fang,Gong Wencheng,Wang Lili,Chen Zilin
Reduced graphene oxide (RGO) was used to construct a bienzyme biosensor containing horseradish peroxidase (HRP) and glucose oxidase (GOx). A poly(toluidine blue) (pTB) film containing RGO acted as both enzyme immobilization matrix and electron transfer mediator. The bienzyme biosensor was characterized by electrochemical techniques and displays a highly sensitive amperometric response to glucose and hydrogen peroxide (H 2 O 2 ) at a potential as low as −0.1 V (vs. SCE). It is shown that use of RGO causes a strong enhancement on the amperometric responses. H 2 O 2 formed by the action of GOx in the presence of oxygen can be further reduced by HRP in the pTB film contacting the RGO modified electrode. In the absence of oxygen, glucose oxidation proceeds by another mechanism in which electron transfer occurs from GOx to the electrode and with pTB acting as the mediator. Amperometric responses to glucose and H 2 O 2 follow Michaelis-Menten kinetics. The experimental conditions were optimized, and under these conditions glucose can be determined in the 80 μM to 3.0 mM range with a detection limit of 50 μM. H 2 O 2 , in turn, can be quantified in up to 30.0 μM concentration with a detection limit of 0.2 μM. The bienzyme biosensor is reproducible, repeatable and stable. Finally, it has been successfully applied to the determination of glucose in plasma samples.
Schematic representation of glocuse detection at GCE/RGO/pTB-HRP-GOx.
研究领域:
电化学
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生物传感器
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材料科学
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分析化学
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产品关联
DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
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2020
·Vol. 167
·期 6
·067502
·
IF 3.721
Junhao Chen,Xiaoyuan Zheng,Yulin Li,Haitao Zheng,Yanjun Liu,Shin-ichiro Suye
Implantable glucose biosensor based on poly (3,4-ethylenedioxythiophene) (PEDOT) modified carbon fiber (CF) microelectrode was fabricated for the first time. Main appeal of this work was the immobilization of glucose oxidase (GOx) on PEDOT film by electrostatic interaction, which resulted in the direct electron transfer (DET) between the active sites of enzyme and electrode in the help of PEDOT. The sensor exhibited selective response to glucose in the presence of some common interference. The biosensor operates at a low potential of −0.65 V vs Ag/AgCl, and the amperometric response was linear over range from 0.5 mM to 15 mM for glucose with a sensitivity of 8.5 μA·cm−2·mM−1. The Michaelis-Menten constant was calculated as 6.5 mM, and the sensor retain 81.3% sensitivity after 60 d storage. Moreover, the extremely simple process of DET construction could be potentially extended for the mass production.
研究领域:
生物传感器
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电化学
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生物医学工程
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材料科学
✓ 已验证
DOI
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
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2023
·
IF 3.8
Minghui Chen,Chen Wang,Fei Chen,Hongjie Li,Yunpeng Wang,Haitao Ma
A highly sensitive and precise electrochemical biosensor for glucose detection was developed by coupling synergetic catalysis of Cu-Zn-Ni oxide films. To enhance the catalytic performance of alloy, potentials between −0.5 V and 1.5 V are selected for potentiostatic oxidation. Notably, the oxidation process reveals the influence of Cl − in the electrolyte, leading to electrode surface etching, with increasing etching and grain boundary collapse at higher potentials. The electrochemical performance of the electrode was comprehensively evaluated using cyclic voltammetry (CV) and amperometric response in alkaline conditions. The catalytic electrode has an impressive linear response in the concentration range of 0.1 ∼ 1.0 mM glucose, with a sensitivity of 3775.11 μA·mM −1 . Furthermore, the electrode exhibits remarkable stability and anti-interference properties, rendering it suitable for non-enzymatic glucose sensing applications.
研究领域:
材料科学
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电化学
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传感器
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生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Zhenning Yu,Lei Zou,Yanli Chen,Jianzhuang Jiang
A facile approach was developed for preparing the multilayer hybrid films of mixed (phthalocyaninato) (porphyrinato) europium(III) triple-decker compound (Pc)Eu(Pc)Eu[trans-T(COOCH3)2PP] (1) and graphene oxide (GO) using the solution-processing QLS method. The combination of the nature of relatively high conductivity and great surface area for GO with the electroactive and semiconductive triple-decker compound in ITO electrode renders the hybrid film excellent sensing property for H2O2, due to the optimized triple-decker molecular packing in the uniform-sized nanoparticles (ca. 70 nm) formed on the GO surface. The amperometric responses are linearly proportional to the concentration of H2O2 in the range of 0.05–1800 μM with a fast response time of 0.03 s μM–1, a low detection limit of 0.017 μM, and good sensitivity of 7.4 μA mM–1. The present work represents the best result of tetrapyrrole-based nonenzymatic electrochemical sensor for H2O2. Nevertheless, the triple-decker/GO/ITO also shows excellent stability, reproducibility, and selectivity, indicating the great potential of electroactive tetrapyrrole rare earth sandwich compounds in combination with GO in the field of nonenzymatic electrochemical sensors.
研究领域:
材料科学
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电化学
·
传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 43
·5613-5622
·
IF 2.378
Xiaojiao Wang,Yang Li,Yuanzhen Zhou,Yijia He
Bisphenol A (BPA) is one of the most prevalent environmental endocrine disrupting chemicals. In this present paper, a facile BPA sensor was successfully fabricated based on a La-doped Yb2O3 nanorod modified carbon paste electrode (La–Yb2O3/CPE). La-doped Yb2O3 nanorods were synthesized using a one step hydrothermal synthesis method and further investigated by X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and amperometric response analysis were applied to characterize the electrochemical properties of the sensor. Under the optimal conditions, the oxidation current increased linearly with increasing the concentration of BPA in the range of 0.04 to 15 μM with a detection limit of 0.013 μM. The prepared La-doped Yb2O3 nanomaterial modified electrode showed good reproducibility, stability and anti-interference ability. Furthermore, this newly developed sensor was further adopted to measure BPA in tap water and milk samples. The possible electrocatalytic sensing mechanism is discussed.
研究领域:
环境科学
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分析化学
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纳米技术
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2015
·Vol. 183
·期 2
·633-642
·
IF 3.741
Fang Shaoming,Dong Xiaodong,Ji Hongfei,Liu Shunli,Yan Fufeng,Peng Donglai,He Linghao,Wang Minghua,Zhang Zhihong
This article describes an aptasensor for lysozyme that is based on a gold electrode modified with an aptamer-wrapped composite consisting of three-dimensional reduced graphene oxide, cuprous oxide, and plasma-polymerized propargylamine (Cu 2 O@rGO@PpPG) as the sensing layer. The nanocomposite consisting of Cu 2 O@rGO was synthesized by simultaneously reducing GO and Cu(II) ions with glucose and then modified by plasma-enhanced chemical vapor deposition using propargylamine as the monomer gas. The resulting amine-rich nanofilms of Cu 2 O@rGO@PpPG nanocomposite were deposited on a gold electrode. Differential pulse voltammetry and electrochemical impedance spectroscopy show these layers to exhibit a good amperometric response and variation of the charge transfer resistance to lysozyme after aptamer strands had been immobilized on the films via electrostatic interaction between the negatively charged phosphate groups of the aptamer and the positively charged amino groups on the electrode. The sensor, when operated at 0.22 V (vs. Ag/AgCl), can detect lysozyme in the 0.1 nM to 200 nM concentration range with a 0.06 nM limit of detection. In addition, the sensor displays excellent selectivity and repeatability. In our perception, this strategy for preparing aptasensors holds a great potential with respect to the use of plasma-modified nanocomposites in clinical analysis.
An effective aptasensor was constructed using a nanocomposite consisting of three-dimensional reduced graphene oxide, cuprous oxide, and plasma-polymerized propargylamine as the sensitive layer. It exhibits a detection limit of 0.06 nM and an analytical range of 0.1 to 200 nM.
研究领域:
分析化学
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纳米技术
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生物传感器
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电化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）